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The map label for this gene is iscS [H]

Identifier: 167039810

GI number: 167039810

Start: 1201145

End: 1202299

Strand: Direct

Name: iscS [H]

Synonym: Teth514_1164

Alternate gene names: 167039810

Gene position: 1201145-1202299 (Clockwise)

Preceding gene: 167039809

Following gene: 167039812

Centisome position: 48.88

GC content: 35.5

Gene sequence:

>1155_bases
GTGGATAGAATTTATTTAGACAATGCTGCGACTACACCAGTTGATAAAAGGGTATTAGAGGCGATGTTACCATATTATAG
TGATGTTTTTGGTAATCCATCTTCTCCGTATTCATATGGTCAAGAAGCGAAAAAAGCTATTGAAGAAGCGAGGGAAAAAG
TAGCAAAAGCACTAGGGGCAGATTCTGAGGAAATATATTTTACCAGTGGAGGGTCTGAGTCAGATAATTGGGCATTAAAA
GGTGTAGCATATGCGTTGAAAGAAAAAGGAAATCATATTATTACAACAGAAATTGAACATCATGCAGTGCTCCATACTTG
CCGATATCTTGAAAAAGAAGGATTCAAAGTAACATATCTTCCAGTTGACGAATATGGACTTGTAAAACCTGAAGATTTAA
AAAAGGCGATTACAGATAAAACAATCCTTGTTTCTATAATGTTTGCTAATAATGAGATAGGTACGATAGAGCCAATTGAG
GAGCTTGTCAAAATAGCTCATGAGAAAAATGTATATTTTCATACTGATGCTGTGCAAGCAGTTGGAAATGTACCGATAGA
TGTAAAAAAATTAGATGTTGATTTGTTATCTTTGTCTGCTCATAAGATATATGGACCCAAAGGTGTAGGAGCATTATATA
TAAAAAAGGGTGTAAAGATTCATTCGCTTATACAAGGTGGAACACAGGAAAGGAATAGAAGAGCGGGCACTGAAAATGTT
CCAGGAATAGTAGGACTGGGGGAAGCGATAGAGCTTATCACGAAAAATTTGGATTCTCATATAAATAAACTCAAATTTTT
AAGAGATAAACTTATAAATGGAATATTAGAAAAAATACCATATGCGAGGTTAAACGGGCATCCAACAAAAAGGCTTCCAG
GCAATGTTAATGTATCATTTGAATTTATAGATGGCGAATCCCTTATTTTAAACTTAGATATGGCAGGAATATGTGCTTCA
AGTGGTTCAGCATGCACTTCTGGTTCACTTGAGCCATCTCATGTGCTTCTGGCAATTGGGCTTTCTCGCGAATTAGCTCG
AGGGTCATTAAGACTTACAATAGGTAAAGAAAACACGGAAGAAGATATAGATAAGGTTTTAGAAGTACTTCCACAAATTG
TTAAAAGATTAAGGTCAATATCACAGATTGTATGA

Upstream 100 bases:

>100_bases
TGGATTTATTCCTGACACATTAAATCTTGACATTATCGATGAAATTATAAAGGTAAGAGAGATACTAAGAAGTAAAAAAT
AATAGAGAGAGGCGATTTTT

Downstream 100 bases:

>100_bases
GAGAATCTACAAGTATTTCGGTAATATGTCAAGAGGGTGGTAAAAAAAATTCAAAAAAAATTTAATAAAATAACCTAAAA
AAAGGGTATAGCAAAGCTAA

Product: cysteine desulfurase

Products: NA

Alternate protein names: NifS protein homolog [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MDRIYLDNAATTPVDKRVLEAMLPYYSDVFGNPSSPYSYGQEAKKAIEEAREKVAKALGADSEEIYFTSGGSESDNWALK
GVAYALKEKGNHIITTEIEHHAVLHTCRYLEKEGFKVTYLPVDEYGLVKPEDLKKAITDKTILVSIMFANNEIGTIEPIE
ELVKIAHEKNVYFHTDAVQAVGNVPIDVKKLDVDLLSLSAHKIYGPKGVGALYIKKGVKIHSLIQGGTQERNRRAGTENV
PGIVGLGEAIELITKNLDSHINKLKFLRDKLINGILEKIPYARLNGHPTKRLPGNVNVSFEFIDGESLILNLDMAGICAS
SGSACTSGSLEPSHVLLAIGLSRELARGSLRLTIGKENTEEDIDKVLEVLPQIVKRLRSISQIV

Sequences:

>Translated_384_residues
MDRIYLDNAATTPVDKRVLEAMLPYYSDVFGNPSSPYSYGQEAKKAIEEAREKVAKALGADSEEIYFTSGGSESDNWALK
GVAYALKEKGNHIITTEIEHHAVLHTCRYLEKEGFKVTYLPVDEYGLVKPEDLKKAITDKTILVSIMFANNEIGTIEPIE
ELVKIAHEKNVYFHTDAVQAVGNVPIDVKKLDVDLLSLSAHKIYGPKGVGALYIKKGVKIHSLIQGGTQERNRRAGTENV
PGIVGLGEAIELITKNLDSHINKLKFLRDKLINGILEKIPYARLNGHPTKRLPGNVNVSFEFIDGESLILNLDMAGICAS
SGSACTSGSLEPSHVLLAIGLSRELARGSLRLTIGKENTEEDIDKVLEVLPQIVKRLRSISQIV
>Mature_384_residues
MDRIYLDNAATTPVDKRVLEAMLPYYSDVFGNPSSPYSYGQEAKKAIEEAREKVAKALGADSEEIYFTSGGSESDNWALK
GVAYALKEKGNHIITTEIEHHAVLHTCRYLEKEGFKVTYLPVDEYGLVKPEDLKKAITDKTILVSIMFANNEIGTIEPIE
ELVKIAHEKNVYFHTDAVQAVGNVPIDVKKLDVDLLSLSAHKIYGPKGVGALYIKKGVKIHSLIQGGTQERNRRAGTENV
PGIVGLGEAIELITKNLDSHINKLKFLRDKLINGILEKIPYARLNGHPTKRLPGNVNVSFEFIDGESLILNLDMAGICAS
SGSACTSGSLEPSHVLLAIGLSRELARGSLRLTIGKENTEEDIDKVLEVLPQIVKRLRSISQIV

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=383, Percent_Identity=48.5639686684073, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI156713448, Length=412, Percent_Identity=35.1941747572816, Blast_Score=231, Evalue=6e-61,
Organism=Escherichia coli, GI48994898, Length=385, Percent_Identity=47.012987012987, Blast_Score=348, Evalue=3e-97,
Organism=Escherichia coli, GI1789175, Length=393, Percent_Identity=28.7531806615776, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1787970, Length=396, Percent_Identity=27.2727272727273, Blast_Score=125, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI25143064, Length=384, Percent_Identity=50.2604166666667, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17533177, Length=330, Percent_Identity=32.7272727272727, Blast_Score=172, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6319831, Length=383, Percent_Identity=49.3472584856397, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI20129463, Length=383, Percent_Identity=48.5639686684073, Blast_Score=363, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42199; Mature: 42199

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRIYLDNAATTPVDKRVLEAMLPYYSDVFGNPSSPYSYGQEAKKAIEEAREKVAKALGA
CCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
DSEEIYFTSGGSESDNWALKGVAYALKEKGNHIITTEIEHHAVLHTCRYLEKEGFKVTYL
CCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCEEEEE
PVDEYGLVKPEDLKKAITDKTILVSIMFANNEIGTIEPIEELVKIAHEKNVYFHTDAVQA
ECCCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCEEEECHHHHH
VGNVPIDVKKLDVDLLSLSAHKIYGPKGVGALYIKKGVKIHSLIQGGTQERNRRAGTENV
HCCCCCEEEEECHHHEEHHHHHCCCCCCCCEEEEECCCCHHHHHCCCCHHHHHCCCCCCC
PGIVGLGEAIELITKNLDSHINKLKFLRDKLINGILEKIPYARLNGHPTKRLPGNVNVSF
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEE
EFIDGESLILNLDMAGICASSGSACTSGSLEPSHVLLAIGLSRELARGSLRLTIGKENTE
EEECCCEEEEEECHHHEECCCCCCCCCCCCCCCEEEEEECCCHHHCCCCEEEEECCCCCH
EDIDKVLEVLPQIVKRLRSISQIV
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDRIYLDNAATTPVDKRVLEAMLPYYSDVFGNPSSPYSYGQEAKKAIEEAREKVAKALGA
CCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
DSEEIYFTSGGSESDNWALKGVAYALKEKGNHIITTEIEHHAVLHTCRYLEKEGFKVTYL
CCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCEEEEE
PVDEYGLVKPEDLKKAITDKTILVSIMFANNEIGTIEPIEELVKIAHEKNVYFHTDAVQA
ECCCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCEEEECHHHHH
VGNVPIDVKKLDVDLLSLSAHKIYGPKGVGALYIKKGVKIHSLIQGGTQERNRRAGTENV
HCCCCCEEEEECHHHEEHHHHHCCCCCCCCEEEEECCCCHHHHHCCCCHHHHHCCCCCCC
PGIVGLGEAIELITKNLDSHINKLKFLRDKLINGILEKIPYARLNGHPTKRLPGNVNVSF
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEE
EFIDGESLILNLDMAGICASSGSACTSGSLEPSHVLLAIGLSRELARGSLRLTIGKENTE
EEECCCEEEEEECHHHEECCCCCCCCCCCCCCCEEEEEECCCHHHCCCCEEEEECCCCCH
EDIDKVLEVLPQIVKRLRSISQIV
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10930739 [H]