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The map label for this gene is rsmF [H]

Identifier: 167039631

GI number: 167039631

Start: 1000723

End: 1002105

Strand: Direct

Name: rsmF [H]

Synonym: Teth514_0979

Alternate gene names: 167039631

Gene position: 1000723-1002105 (Clockwise)

Preceding gene: 167039630

Following gene: 167039632

Centisome position: 40.73

GC content: 34.85

Gene sequence:

>1383_bases
ATGAAAATTACAGTAAAAAAAGAGTTTTTGAAGAGAATGGAGCAATTACTTAAAGAAGAGTATGAAGATTTTTTAAAGGA
ATACGACAAAAAGCCTCAAAAAGCCTTAAGAGTGAATACATTAAAAATCGGTGTAGAAGAGTTTAAAAAAATTGTTCCTT
TTGAGATAACCCCAATTCCTTGGTGCCCAACTGGGTTTTATTATGATAAAGAAGAAAAGGCGGGCAACCATTTGTACCAT
ATTTTAGGCCTTTACTATATTCAAGAACCGACAGCGATGGCAGTTGTGGAGGTATTAGACCCTAGGCCGGGGGAAAAGAT
TCTCGATGTAAGTGCAGCGCCGGGAGGTAAAACCACCCACATAGCTACGAAAATTGGCGATGAAGGAATAATTGTTGCTA
ATGAAATAGATAAAAAAAGAATAAAAGCACTGGTGGAAAATGTAGAGAGAATGGGAATAAGAAATATTGTCATAACAAAT
GAAACTCCTGAAAAGTTGGCTACCGCATTTGAAGGATATTTTGATAAAATTTTGGTAGATGCTCCTTGCTCAGGAGAGGG
AATGTTTAGAAAAGACCCTACAGCAAGGAAAATTTGGTCTTTAAATAATGTCATGAGTTGTTCTATTACACAGAAAAATA
TACTTAGAAATGTAGTATCCTTATTAAAGCCAGGAGGTATTTTAGTCTATTCTACTTGCACTTTTTCGCCAGAAGAAAAC
GAAGGGGTTATAAAAAATTTTTTAGAAGAATATCCGGAGTTTGAAGTTGAAGAAAGTGAAATGAGCAAATTCTTTGACAG
AGGGCATCCCGAATGGGTTGAAGGTCCTGAAGATTTAAAAAAATGCATGAGATTATGGCCACATAAGGTGAGAGGAGAAG
GACATTTTATTGCGAAACTGAGAAAAAAAGAGGAAGAAGAGCCAGACTCTAGATTTAAAAAAACAAAAGGCAGAAAAAAG
CAAAAAGAATTACAACTATTTTACGATTTTGCTGATAAATATCTTAATTTAGACTTAGATAATCTAAATATTGAAGTGCA
TAATGATTTTGTTTACCATGTACCAGAGGGTTTACCGGATTTGAGTAATATAAAGGTATACAGATACGGTTGGCAGTTAG
GAGAGCTTAAGAAAAACAGGTTTGAGCCTTCTCACTGGTTGGCAATGGGAATTAAAACTTTCCAAGCTAAAAGACTTTTG
GAACTTGAAAGAGAACAAAGGGAAAAATATTTAAAAGGTGAAACTTTTGAATTGGACTTAGAAGACGGATGGATTTTTTT
TACTATAGAAGGGTTTCCTGCGGGATGGGGCAGAATTGCAAAAGGGGTAGTTAAAAATTATTATCCTAAGTGGCTTAGAG
ATATAAAAGTTTGGGAAGAATAG

Upstream 100 bases:

>100_bases
AAATATTATTATCAGCTGAAAATAATGGGAAAGTTATTAGTAATCTAAAAGATTAGTTGTCAATATATTATTCAATCAAT
AAGATAGGGGTAGTAATAAG

Downstream 100 bases:

>100_bases
TTGTGTAATGCTCGCGTATGCGAGCTTTTATTTTTATGCTTTAAAATCTCAATTTTGGGGGAATAATATTTTTAGCTGAA
ATTGCAGTGGAGAAGGAGTT

Product: NOL1/NOP2/sun family RNA methylase

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MKITVKKEFLKRMEQLLKEEYEDFLKEYDKKPQKALRVNTLKIGVEEFKKIVPFEITPIPWCPTGFYYDKEEKAGNHLYH
ILGLYYIQEPTAMAVVEVLDPRPGEKILDVSAAPGGKTTHIATKIGDEGIIVANEIDKKRIKALVENVERMGIRNIVITN
ETPEKLATAFEGYFDKILVDAPCSGEGMFRKDPTARKIWSLNNVMSCSITQKNILRNVVSLLKPGGILVYSTCTFSPEEN
EGVIKNFLEEYPEFEVEESEMSKFFDRGHPEWVEGPEDLKKCMRLWPHKVRGEGHFIAKLRKKEEEEPDSRFKKTKGRKK
QKELQLFYDFADKYLNLDLDNLNIEVHNDFVYHVPEGLPDLSNIKVYRYGWQLGELKKNRFEPSHWLAMGIKTFQAKRLL
ELEREQREKYLKGETFELDLEDGWIFFTIEGFPAGWGRIAKGVVKNYYPKWLRDIKVWEE

Sequences:

>Translated_460_residues
MKITVKKEFLKRMEQLLKEEYEDFLKEYDKKPQKALRVNTLKIGVEEFKKIVPFEITPIPWCPTGFYYDKEEKAGNHLYH
ILGLYYIQEPTAMAVVEVLDPRPGEKILDVSAAPGGKTTHIATKIGDEGIIVANEIDKKRIKALVENVERMGIRNIVITN
ETPEKLATAFEGYFDKILVDAPCSGEGMFRKDPTARKIWSLNNVMSCSITQKNILRNVVSLLKPGGILVYSTCTFSPEEN
EGVIKNFLEEYPEFEVEESEMSKFFDRGHPEWVEGPEDLKKCMRLWPHKVRGEGHFIAKLRKKEEEEPDSRFKKTKGRKK
QKELQLFYDFADKYLNLDLDNLNIEVHNDFVYHVPEGLPDLSNIKVYRYGWQLGELKKNRFEPSHWLAMGIKTFQAKRLL
ELEREQREKYLKGETFELDLEDGWIFFTIEGFPAGWGRIAKGVVKNYYPKWLRDIKVWEE
>Mature_460_residues
MKITVKKEFLKRMEQLLKEEYEDFLKEYDKKPQKALRVNTLKIGVEEFKKIVPFEITPIPWCPTGFYYDKEEKAGNHLYH
ILGLYYIQEPTAMAVVEVLDPRPGEKILDVSAAPGGKTTHIATKIGDEGIIVANEIDKKRIKALVENVERMGIRNIVITN
ETPEKLATAFEGYFDKILVDAPCSGEGMFRKDPTARKIWSLNNVMSCSITQKNILRNVVSLLKPGGILVYSTCTFSPEEN
EGVIKNFLEEYPEFEVEESEMSKFFDRGHPEWVEGPEDLKKCMRLWPHKVRGEGHFIAKLRKKEEEEPDSRFKKTKGRKK
QKELQLFYDFADKYLNLDLDNLNIEVHNDFVYHVPEGLPDLSNIKVYRYGWQLGELKKNRFEPSHWLAMGIKTFQAKRLL
ELEREQREKYLKGETFELDLEDGWIFFTIEGFPAGWGRIAKGVVKNYYPKWLRDIKVWEE

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=292, Percent_Identity=31.1643835616438, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI76150623, Length=292, Percent_Identity=31.1643835616438, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI32698918, Length=236, Percent_Identity=33.8983050847458, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI39995082, Length=290, Percent_Identity=28.2758620689655, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI301336155, Length=290, Percent_Identity=28.2758620689655, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI11545785, Length=179, Percent_Identity=34.6368715083799, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI8922322, Length=259, Percent_Identity=25.0965250965251, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI270288818, Length=252, Percent_Identity=26.1904761904762, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI23199998, Length=252, Percent_Identity=26.1904761904762, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI270288816, Length=252, Percent_Identity=26.1904761904762, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI87081985, Length=472, Percent_Identity=31.1440677966102, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI2367212, Length=300, Percent_Identity=30.6666666666667, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17536757, Length=300, Percent_Identity=31.6666666666667, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI25143471, Length=307, Percent_Identity=28.0130293159609, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324268, Length=336, Percent_Identity=30.0595238095238, Blast_Score=146, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6319447, Length=272, Percent_Identity=29.0441176470588, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI22024126, Length=332, Percent_Identity=31.9277108433735, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI21355201, Length=317, Percent_Identity=27.7602523659306, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24668781, Length=233, Percent_Identity=33.0472103004292, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21356513, Length=233, Percent_Identity=33.0472103004292, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19920494, Length=162, Percent_Identity=31.4814814814815, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356579, Length=234, Percent_Identity=27.3504273504274, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 53763; Mature: 53763

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITVKKEFLKRMEQLLKEEYEDFLKEYDKKPQKALRVNTLKIGVEEFKKIVPFEITPIP
CEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEECHHHHHHCCCEEECCCC
WCPTGFYYDKEEKAGNHLYHILGLYYIQEPTAMAVVEVLDPRPGEKILDVSAAPGGKTTH
CCCCCCEECCHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCEE
IATKIGDEGIIVANEIDKKRIKALVENVERMGIRNIVITNETPEKLATAFEGYFDKILVD
EEEEECCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHEEEE
APCSGEGMFRKDPTARKIWSLNNVMSCSITQKNILRNVVSLLKPGGILVYSTCTFSPEEN
CCCCCCCCCCCCCCHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCC
EGVIKNFLEEYPEFEVEESEMSKFFDRGHPEWVEGPEDLKKCMRLWPHKVRGEGHFIAKL
CCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHCCCCHHHHHH
RKKEEEEPDSRFKKTKGRKKQKELQLFYDFADKYLNLDLDNLNIEVHNDFVYHVPEGLPD
HHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEECCEEEEEECCEEEECCCCCCC
LSNIKVYRYGWQLGELKKNRFEPSHWLAMGIKTFQAKRLLELEREQREKYLKGETFELDL
CCCEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
EDGWIFFTIEGFPAGWGRIAKGVVKNYYPKWLRDIKVWEE
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKITVKKEFLKRMEQLLKEEYEDFLKEYDKKPQKALRVNTLKIGVEEFKKIVPFEITPIP
CEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEECHHHHHHCCCEEECCCC
WCPTGFYYDKEEKAGNHLYHILGLYYIQEPTAMAVVEVLDPRPGEKILDVSAAPGGKTTH
CCCCCCEECCHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCEE
IATKIGDEGIIVANEIDKKRIKALVENVERMGIRNIVITNETPEKLATAFEGYFDKILVD
EEEEECCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHEEEE
APCSGEGMFRKDPTARKIWSLNNVMSCSITQKNILRNVVSLLKPGGILVYSTCTFSPEEN
CCCCCCCCCCCCCCHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCC
EGVIKNFLEEYPEFEVEESEMSKFFDRGHPEWVEGPEDLKKCMRLWPHKVRGEGHFIAKL
CCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHCCCCHHHHHH
RKKEEEEPDSRFKKTKGRKKQKELQLFYDFADKYLNLDLDNLNIEVHNDFVYHVPEGLPD
HHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEECCEEEEEECCEEEECCCCCCC
LSNIKVYRYGWQLGELKKNRFEPSHWLAMGIKTFQAKRLLELEREQREKYLKGETFELDL
CCCEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
EDGWIFFTIEGFPAGWGRIAKGVVKNYYPKWLRDIKVWEE
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA