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The map label for this gene is cwlD [H]

Identifier: 167039578

GI number: 167039578

Start: 947919

End: 948578

Strand: Direct

Name: cwlD [H]

Synonym: Teth514_0926

Alternate gene names: 167039578

Gene position: 947919-948578 (Clockwise)

Preceding gene: 167039576

Following gene: 167039579

Centisome position: 38.58

GC content: 31.21

Gene sequence:

>660_bases
ATGTTTTTACTAATTGTAAGTTTAATAGTAGGGTTATATAGTTTTAAAAAAGGGTATTATATAGCTGCTTTTAAAACTGT
TCCAATAATGAATAAAGTTATTGTCATAGATGCGGGACATGGAGGACCGGACCCTGGGAAACCTGGAAAATATGGAAAAG
ATGAGGATGAGTTAAATCTTGAGATTGCTCAAAAATTAAGAGAACTTATTGAAGAAAGTGGTGGGATTGTAGTGATGACA
AGGGAAGACGACACTTTGTCAGATAGCTCTCTTTCAAAGGATTTAAAAAATAGAGTGGTAAAAGCAAATGAAGTAATAGC
AGATGTTTTAATTAGTATTCATTTAAATAGCTTTAGTCAATCTAAATACAAAGGGGCACAGGTATTTTATCAAAACAATT
CTGAAAAAGGTAAACTATTAGCAGAACTTATTCAACAAGAGTTAAGAAATACGTTAGATCCGAATAACGACAGGATGGCT
AAAAGTTCTAATAGTTATTACCTTTTGCGAAACGCTAAAATGCCGGCAGTTATTGTGGAATGTGGCTTTATGTCAAATCC
TGAAGAAGAAAAACTTTTAAATGACGAAAATTATCAATATAAAATTGCTTGGGCAATATATAAAGGTTTAATACATTATT
TTCAAAAAGTTTCAGAATAG

Upstream 100 bases:

>100_bases
ATATGTGGATTTTATTATTAAGTGACATATAAACTTTTTTAGATGAATAATTTTTACTAAAAGATAAGGGGATGGGGACA
TGGTATTTAAAAGGTGGATG

Downstream 100 bases:

>100_bases
AAAAATTTTTCACTTCATGTCTTGTTATGAAGTGATTTTTTTTGTATACTAAAAGAAGAAGAGAATTTATGAAGGGAGAT
AATATGGGGATAAAAACAGT

Product: cell wall hydrolase/autolysin

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MFLLIVSLIVGLYSFKKGYYIAAFKTVPIMNKVIVIDAGHGGPDPGKPGKYGKDEDELNLEIAQKLRELIEESGGIVVMT
REDDTLSDSSLSKDLKNRVVKANEVIADVLISIHLNSFSQSKYKGAQVFYQNNSEKGKLLAELIQQELRNTLDPNNDRMA
KSSNSYYLLRNAKMPAVIVECGFMSNPEEEKLLNDENYQYKIAWAIYKGLIHYFQKVSE

Sequences:

>Translated_219_residues
MFLLIVSLIVGLYSFKKGYYIAAFKTVPIMNKVIVIDAGHGGPDPGKPGKYGKDEDELNLEIAQKLRELIEESGGIVVMT
REDDTLSDSSLSKDLKNRVVKANEVIADVLISIHLNSFSQSKYKGAQVFYQNNSEKGKLLAELIQQELRNTLDPNNDRMA
KSSNSYYLLRNAKMPAVIVECGFMSNPEEEKLLNDENYQYKIAWAIYKGLIHYFQKVSE
>Mature_219_residues
MFLLIVSLIVGLYSFKKGYYIAAFKTVPIMNKVIVIDAGHGGPDPGKPGKYGKDEDELNLEIAQKLRELIEESGGIVVMT
REDDTLSDSSLSKDLKNRVVKANEVIADVLISIHLNSFSQSKYKGAQVFYQNNSEKGKLLAELIQQELRNTLDPNNDRMA
KSSNSYYLLRNAKMPAVIVECGFMSNPEEEKLLNDENYQYKIAWAIYKGLIHYFQKVSE

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=227, Percent_Identity=31.7180616740088, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI87082163, Length=225, Percent_Identity=28.8888888888889, Blast_Score=91, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR014234 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 24737; Mature: 24737

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLLIVSLIVGLYSFKKGYYIAAFKTVPIMNKVIVIDAGHGGPDPGKPGKYGKDEDELNL
CHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCHHHCCH
EIAQKLRELIEESGGIVVMTREDDTLSDSSLSKDLKNRVVKANEVIADVLISIHLNSFSQ
HHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SKYKGAQVFYQNNSEKGKLLAELIQQELRNTLDPNNDRMAKSSNSYYLLRNAKMPAVIVE
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCEEEEE
CGFMSNPEEEKLLNDENYQYKIAWAIYKGLIHYFQKVSE
ECCCCCCCHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFLLIVSLIVGLYSFKKGYYIAAFKTVPIMNKVIVIDAGHGGPDPGKPGKYGKDEDELNL
CHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCHHHCCH
EIAQKLRELIEESGGIVVMTREDDTLSDSSLSKDLKNRVVKANEVIADVLISIHLNSFSQ
HHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SKYKGAQVFYQNNSEKGKLLAELIQQELRNTLDPNNDRMAKSSNSYYLLRNAKMPAVIVE
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCEEEEE
CGFMSNPEEEKLLNDENYQYKIAWAIYKGLIHYFQKVSE
ECCCCCCCHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7559346; 8969501; 9384377 [H]