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The map label for this gene is tufA [H]

Identifier: 167039518

GI number: 167039518

Start: 903449

End: 904651

Strand: Direct

Name: tufA [H]

Synonym: Teth514_0865

Alternate gene names: 167039518

Gene position: 903449-904651 (Clockwise)

Preceding gene: 167039517

Following gene: 167039519

Centisome position: 36.77

GC content: 46.05

Gene sequence:

>1203_bases
ATGGCGAAGCAAAAATTTGAGAGGAAGAAGCCGCACGTAAACGTAGGGACGATAGGTCACGTAGACCATGGTAAGACGAC
ATTGACAGCAGCGATAACGATGGTATTATCGAAGGCAGGGATGGCAGAGGCGAAGGGGTATGACGAGATAGACAAGGCGC
CGGAGGAGAGAGCAAGGGGAATAACAATAAACACGACTCACGTAGAATATGAGACAGAGAAGAGGCACTATGCGCACGTA
GACTGTCCAGGTCACGCAGACTATGTAAAGAACATGATAACAGGGGCAGCGCAGATGGATGGAGCGATATTGGTAGTATC
AGCAGCAGACGGTCCGATGCCACAGACGAGGGAGCACATATTATTGGCGAGGCAGGTAGGAGTGCCATATATAGTAGTAT
TTTTAAACAAGGCAGACATGGTAGACGATCCAGAATTAATAGAATTAGTAGAGATGGAAGTAAGGGAATTATTAAACGAA
TATGAATTTCCTGGGGATGACACACCGATAGTAGTAGGATCAGCATTAAAGGCATTAGAATGTGGATGTGGCAAGAGAGA
GTGTGAATGGTGCGGTAAGATATGGCAGTTAATGGATATAGTAGACGAATATATACCGACACCGGAGAGAGACATAGACA
AGCCATTTTTGATGCCAGTAGAGGACGTATTTACGATAACAGGAAGGGGAACTGTTGCGACAGGAAGAGTAGAGAGAGGG
AAGGTAAAGGTAGGAGACGAAGTAGAGATAATAGGATTGACGACAGAATCGAGGAAGACAGTAGTAACAGGAGTAGAGAT
GTTCAGGGAGACGATGGATGAAGCGCAAGCAGGGGACAACATAGGAGTATTATTAAGAGGAGTACAGAGGGACGAAGTAG
AGAGAGGGCAAGTATTAGCGAAGCCAGGGACGATAAAGCCGCACACGAAATTTGAGGCTCAAGTTTACGTATTGACGAAG
GAAGAGGGAGGAAGGCACACACCATTTTTCAATGGATACAGGCCACAATTTTACTTTAGGACGACAGACGTGACAGGGGT
AATAAACCTGCCGGATGGAGTAGAGATGGTGATGCCAGGTGACCACGTAACGATAAAAGTGGAGTTAATAACACCGATAG
CGATGGAAGAAGGTTTGAAGTTTGCTATAAGAGAAGGCGGCCGTACAGTAGGTGCTGGCGTCGTGTCAGCTATCATAGAA
TAA

Upstream 100 bases:

>100_bases
AAGGAAGAGGAACTTACACTATGCAGTTCCATCATTATGAAGAAGTTCCTAAAAATATTGCTGATCAAATATTAGAAAAG
AAGTAAAGGAGGAAAAGAGG

Downstream 100 bases:

>100_bases
CATCATATTTTTGTGCTAATAGCAGTTGCTATTAGCACAAAAATATTGATATGTCAAGTCTTGCAATGATTTAGAACTTG
AGATAAAATATTAAAGCTGG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu-A [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MAKQKFERKKPHVNVGTIGHVDHGKTTLTAAITMVLSKAGMAEAKGYDEIDKAPEERARGITINTTHVEYETEKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDPELIELVEMEVRELLNE
YEFPGDDTPIVVGSALKALECGCGKRECEWCGKIWQLMDIVDEYIPTPERDIDKPFLMPVEDVFTITGRGTVATGRVERG
KVKVGDEVEIIGLTTESRKTVVTGVEMFRETMDEAQAGDNIGVLLRGVQRDEVERGQVLAKPGTIKPHTKFEAQVYVLTK
EEGGRHTPFFNGYRPQFYFRTTDVTGVINLPDGVEMVMPGDHVTIKVELITPIAMEEGLKFAIREGGRTVGAGVVSAIIE

Sequences:

>Translated_400_residues
MAKQKFERKKPHVNVGTIGHVDHGKTTLTAAITMVLSKAGMAEAKGYDEIDKAPEERARGITINTTHVEYETEKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDPELIELVEMEVRELLNE
YEFPGDDTPIVVGSALKALECGCGKRECEWCGKIWQLMDIVDEYIPTPERDIDKPFLMPVEDVFTITGRGTVATGRVERG
KVKVGDEVEIIGLTTESRKTVVTGVEMFRETMDEAQAGDNIGVLLRGVQRDEVERGQVLAKPGTIKPHTKFEAQVYVLTK
EEGGRHTPFFNGYRPQFYFRTTDVTGVINLPDGVEMVMPGDHVTIKVELITPIAMEEGLKFAIREGGRTVGAGVVSAIIE
>Mature_399_residues
AKQKFERKKPHVNVGTIGHVDHGKTTLTAAITMVLSKAGMAEAKGYDEIDKAPEERARGITINTTHVEYETEKRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDPELIELVEMEVRELLNEY
EFPGDDTPIVVGSALKALECGCGKRECEWCGKIWQLMDIVDEYIPTPERDIDKPFLMPVEDVFTITGRGTVATGRVERGK
VKVGDEVEIIGLTTESRKTVVTGVEMFRETMDEAQAGDNIGVLLRGVQRDEVERGQVLAKPGTIKPHTKFEAQVYVLTKE
EGGRHTPFFNGYRPQFYFRTTDVTGVINLPDGVEMVMPGDHVTIKVELITPIAMEEGLKFAIREGGRTVGAGVVSAIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=57.9746835443038, Blast_Score=479, Evalue=1e-135,
Organism=Homo sapiens, GI223555963, Length=437, Percent_Identity=29.9771167048055, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI5729864, Length=437, Percent_Identity=29.9771167048055, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI4503471, Length=446, Percent_Identity=30.0448430493274, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI4503475, Length=447, Percent_Identity=28.8590604026846, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI194018522, Length=349, Percent_Identity=28.6532951289398, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI194097354, Length=349, Percent_Identity=28.6532951289398, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI194018520, Length=349, Percent_Identity=28.6532951289398, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI46094014, Length=387, Percent_Identity=27.6485788113695, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI54607086, Length=352, Percent_Identity=28.9772727272727, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI4503507, Length=279, Percent_Identity=27.9569892473118, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI157426893, Length=242, Percent_Identity=26.8595041322314, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1790412, Length=400, Percent_Identity=74.5, Blast_Score=597, Evalue=1e-172,
Organism=Escherichia coli, GI1789737, Length=399, Percent_Identity=74.6867167919799, Blast_Score=596, Evalue=1e-172,
Organism=Escherichia coli, GI2367247, Length=341, Percent_Identity=30.4985337243402, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI48994988, Length=280, Percent_Identity=31.4285714285714, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1789108, Length=371, Percent_Identity=28.0323450134771, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788922, Length=141, Percent_Identity=36.1702127659575, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790835, Length=176, Percent_Identity=28.4090909090909, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=51.7412935323383, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI25141371, Length=392, Percent_Identity=44.3877551020408, Blast_Score=334, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI17552884, Length=454, Percent_Identity=29.5154185022026, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17569207, Length=454, Percent_Identity=29.5154185022026, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI32566303, Length=428, Percent_Identity=28.9719626168224, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=442, Percent_Identity=27.3755656108597, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=439, Percent_Identity=27.3348519362187, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI32566629, Length=457, Percent_Identity=27.1334792122538, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=304, Percent_Identity=30.2631578947368, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=37.5838926174497, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=282, Percent_Identity=26.9503546099291, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=237, Percent_Identity=28.2700421940928, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=399, Percent_Identity=65.9147869674186, Blast_Score=540, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6325337, Length=445, Percent_Identity=30.561797752809, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6319594, Length=445, Percent_Identity=30.561797752809, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6320377, Length=346, Percent_Identity=28.6127167630058, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322937, Length=354, Percent_Identity=24.5762711864407, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=299, Percent_Identity=26.7558528428094, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=244, Percent_Identity=29.9180327868852, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=171, Percent_Identity=30.9941520467836, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281363316, Length=399, Percent_Identity=57.6441102756892, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI17864358, Length=399, Percent_Identity=57.6441102756892, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI19921738, Length=390, Percent_Identity=49.4871794871795, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI45553807, Length=454, Percent_Identity=30.3964757709251, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI45553816, Length=454, Percent_Identity=30.3964757709251, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24651721, Length=454, Percent_Identity=30.3964757709251, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI17864154, Length=454, Percent_Identity=30.3964757709251, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24652838, Length=444, Percent_Identity=29.5045045045045, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI17137572, Length=444, Percent_Identity=29.5045045045045, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI19922690, Length=309, Percent_Identity=31.7152103559871, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI45550900, Length=450, Percent_Identity=29.3333333333333, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI17137380, Length=347, Percent_Identity=30.5475504322767, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24647048, Length=428, Percent_Identity=26.6355140186916, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24647046, Length=428, Percent_Identity=26.6355140186916, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI28572034, Length=166, Percent_Identity=37.9518072289157, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24582462, Length=143, Percent_Identity=33.5664335664336, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 44149; Mature: 44018

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQKFERKKPHVNVGTIGHVDHGKTTLTAAITMVLSKAGMAEAKGYDEIDKAPEERARG
CCCCHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCHHCCCCCHHHCCHHHHHCC
ITINTTHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIVVFLNKADMVDDPELIELVEMEVRELLNEYEFPGDDTPIVVGSALKALE
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHH
CGCGKRECEWCGKIWQLMDIVDEYIPTPERDIDKPFLMPVEDVFTITGRGTVATGRVERG
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEECCCEEEECCEECC
KVKVGDEVEIIGLTTESRKTVVTGVEMFRETMDEAQAGDNIGVLLRGVQRDEVERGQVLA
EEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCCEEE
KPGTIKPHTKFEAQVYVLTKEEGGRHTPFFNGYRPQFYFRTTDVTGVINLPDGVEMVMPG
CCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECC
DHVTIKVELITPIAMEEGLKFAIREGGRTVGAGVVSAIIE
CEEEEEEEEECCHHHHCCCEEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AKQKFERKKPHVNVGTIGHVDHGKTTLTAAITMVLSKAGMAEAKGYDEIDKAPEERARG
CCCHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCHHCCCCCHHHCCHHHHHCC
ITINTTHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIVVFLNKADMVDDPELIELVEMEVRELLNEYEFPGDDTPIVVGSALKALE
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHH
CGCGKRECEWCGKIWQLMDIVDEYIPTPERDIDKPFLMPVEDVFTITGRGTVATGRVERG
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEECCCEEEECCEECC
KVKVGDEVEIIGLTTESRKTVVTGVEMFRETMDEAQAGDNIGVLLRGVQRDEVERGQVLA
EEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCCEEE
KPGTIKPHTKFEAQVYVLTKEEGGRHTPFFNGYRPQFYFRTTDVTGVINLPDGVEMVMPG
CCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECC
DHVTIKVELITPIAMEEGLKFAIREGGRTVGAGVVSAIIE
CEEEEEEEEECCHHHHCCCEEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]