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The map label for this gene is tufA [H]

Identifier: 167039505

GI number: 167039505

Start: 887786

End: 888988

Strand: Direct

Name: tufA [H]

Synonym: Teth514_0851

Alternate gene names: 167039505

Gene position: 887786-888988 (Clockwise)

Preceding gene: 167039504

Following gene: 167039506

Centisome position: 36.13

GC content: 45.55

Gene sequence:

>1203_bases
ATGGCGAAGCAAAAATTTGAGAGGAAGAAGCCGCACGTAAACGTAGGGACGATAGGTCACGTAGACCATGGTAAGACGAC
ATTGACAGCAGCGATAACGATGGTATTATCGAAGGCAGGAATGGCAGAGGCGAAGGGATATGACGAGATAGACAAGGCAC
CAGAGGAGAAAGCAAGGGGAATAACGATAAACACGACTCACGTAGAATATGAGACAGAGAAGAGGCACTATGCGCACGTA
GACTGTCCAGGTCACGCAGACTATGTAAAGAACATGATAACAGGGGCAGCGCAGATGGATGGAGCGATATTGGTAGTATC
AGCAGCAGACGGTCCGATGCCACAGACGAGGGAGCACATATTATTGGCGAGGCAGGTAGGAGTGCCATATATAGTAGTAT
TTTTAAACAAGGCAGACATGGTAGACGATCCAGAATTAATAGAATTAGTAGAGATGGAAGTAAGGGAATTATTAAACGAA
TATGAATTTCCTGGGGATGACACACCGATAGTAGTAGGATCAGCATTAAAGGCATTAGAATGTGGATGTGGCAAGAGAGA
GTGTGAATGGTGCGGTAAGATATGGCAGTTAATGGATATAGTAGACGAATATATACCGACACCGGAGAGAGACATAGACA
AGCCATTTTTGATGCCAGTAGAGGACGTATTTACGATAACAGGAAGGGGAACTGTTGCGACAGGAAGAGTAGAGAGAGGG
AAGGTAAAGGTAGGAGACGAAGTAGAGATAATAGGATTGACGACAGAATCGAGGAAGACAGTAGTAACAGGAGTAGAGAT
GTTCAGGAAGACGATGGATGAAGCGCAAGCAGGGGACAACATAGGAGTATTATTAAGAGGAGTACAGAGGGACGAAGTAG
AGAGAGGGCAAGTATTAGCGAAGCCAGGGACGATAAAGCCGCACACGAAATTTGAGGCTCAAGTTTACGTATTGACGAAG
GAAGAGGGAGGAAGGCACACACCATTTTTCAATGGATACAGGCCACAATTTTACTTTAGGACGACAGACGTGACAGGGGT
AATAAACCTGCCGGATGGAGTAGAGATGGTGATGCCAGGTGACCACGTAACGATAAAAGTAGAGTTAATAACACCGATAG
CGATGGAAGAAGGTTTGAAGTTTGCTATAAGAGAAGGCGGCCGTACAGTAGGTGCTGGCGTCGTGTCAGCTATCATAGAA
TAA

Upstream 100 bases:

>100_bases
TTTTTTAAAGCACACGCACAAAGATGTATAAATTGTCACTTTTGTGACATAATTTTAAGAAACACCAGGATAAGTTTATT
GTGTAAAGGAGGAAAAGAGG

Downstream 100 bases:

>100_bases
AGAGAAGCCGGGTTCAAGCCTGGCTTCTTTTGGGTTAAATCAATGCTTTAGCAAACTGTGCAAAATGTCAACCAATGAGC
TACCTATAAATCTTTGACAC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu-A [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MAKQKFERKKPHVNVGTIGHVDHGKTTLTAAITMVLSKAGMAEAKGYDEIDKAPEEKARGITINTTHVEYETEKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDPELIELVEMEVRELLNE
YEFPGDDTPIVVGSALKALECGCGKRECEWCGKIWQLMDIVDEYIPTPERDIDKPFLMPVEDVFTITGRGTVATGRVERG
KVKVGDEVEIIGLTTESRKTVVTGVEMFRKTMDEAQAGDNIGVLLRGVQRDEVERGQVLAKPGTIKPHTKFEAQVYVLTK
EEGGRHTPFFNGYRPQFYFRTTDVTGVINLPDGVEMVMPGDHVTIKVELITPIAMEEGLKFAIREGGRTVGAGVVSAIIE

Sequences:

>Translated_400_residues
MAKQKFERKKPHVNVGTIGHVDHGKTTLTAAITMVLSKAGMAEAKGYDEIDKAPEEKARGITINTTHVEYETEKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDPELIELVEMEVRELLNE
YEFPGDDTPIVVGSALKALECGCGKRECEWCGKIWQLMDIVDEYIPTPERDIDKPFLMPVEDVFTITGRGTVATGRVERG
KVKVGDEVEIIGLTTESRKTVVTGVEMFRKTMDEAQAGDNIGVLLRGVQRDEVERGQVLAKPGTIKPHTKFEAQVYVLTK
EEGGRHTPFFNGYRPQFYFRTTDVTGVINLPDGVEMVMPGDHVTIKVELITPIAMEEGLKFAIREGGRTVGAGVVSAIIE
>Mature_399_residues
AKQKFERKKPHVNVGTIGHVDHGKTTLTAAITMVLSKAGMAEAKGYDEIDKAPEEKARGITINTTHVEYETEKRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDPELIELVEMEVRELLNEY
EFPGDDTPIVVGSALKALECGCGKRECEWCGKIWQLMDIVDEYIPTPERDIDKPFLMPVEDVFTITGRGTVATGRVERGK
VKVGDEVEIIGLTTESRKTVVTGVEMFRKTMDEAQAGDNIGVLLRGVQRDEVERGQVLAKPGTIKPHTKFEAQVYVLTKE
EGGRHTPFFNGYRPQFYFRTTDVTGVINLPDGVEMVMPGDHVTIKVELITPIAMEEGLKFAIREGGRTVGAGVVSAIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=57.9746835443038, Blast_Score=479, Evalue=1e-135,
Organism=Homo sapiens, GI223555963, Length=437, Percent_Identity=29.5194508009153, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI5729864, Length=437, Percent_Identity=29.5194508009153, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI4503471, Length=446, Percent_Identity=29.5964125560538, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI4503475, Length=447, Percent_Identity=28.4116331096197, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI194018522, Length=349, Percent_Identity=28.3667621776504, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI194097354, Length=349, Percent_Identity=28.3667621776504, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI194018520, Length=349, Percent_Identity=28.3667621776504, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI46094014, Length=387, Percent_Identity=27.390180878553, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI54607086, Length=352, Percent_Identity=28.6931818181818, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI4503507, Length=279, Percent_Identity=27.9569892473118, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI157426893, Length=242, Percent_Identity=26.4462809917355, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1790412, Length=399, Percent_Identity=75.187969924812, Blast_Score=599, Evalue=1e-173,
Organism=Escherichia coli, GI1789737, Length=399, Percent_Identity=75.187969924812, Blast_Score=599, Evalue=1e-172,
Organism=Escherichia coli, GI2367247, Length=341, Percent_Identity=30.791788856305, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI48994988, Length=280, Percent_Identity=31.7857142857143, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789108, Length=371, Percent_Identity=27.7628032345013, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1790835, Length=176, Percent_Identity=28.9772727272727, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788922, Length=141, Percent_Identity=35.4609929078014, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=52.2388059701493, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25141371, Length=394, Percent_Identity=44.6700507614213, Blast_Score=337, Evalue=6e-93,
Organism=Caenorhabditis elegans, GI17552884, Length=454, Percent_Identity=29.0748898678414, Blast_Score=161, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=454, Percent_Identity=29.0748898678414, Blast_Score=161, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI32566303, Length=428, Percent_Identity=28.5046728971963, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=439, Percent_Identity=27.1070615034169, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI115532067, Length=442, Percent_Identity=27.1493212669683, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI32566629, Length=443, Percent_Identity=27.0880361173815, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=304, Percent_Identity=30.2631578947368, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=36.9127516778523, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=279, Percent_Identity=26.5232974910394, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=237, Percent_Identity=27.8481012658228, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=399, Percent_Identity=65.9147869674186, Blast_Score=540, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6325337, Length=445, Percent_Identity=30.1123595505618, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=445, Percent_Identity=30.1123595505618, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6320377, Length=346, Percent_Identity=28.0346820809249, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322937, Length=354, Percent_Identity=24.0112994350282, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=299, Percent_Identity=26.7558528428094, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=285, Percent_Identity=28.0701754385965, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=171, Percent_Identity=30.4093567251462, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI281363316, Length=399, Percent_Identity=57.8947368421053, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI17864358, Length=399, Percent_Identity=57.8947368421053, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI19921738, Length=390, Percent_Identity=50, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI45553807, Length=454, Percent_Identity=29.9559471365639, Blast_Score=157, Evalue=8e-39,
Organism=Drosophila melanogaster, GI45553816, Length=454, Percent_Identity=29.9559471365639, Blast_Score=157, Evalue=8e-39,
Organism=Drosophila melanogaster, GI24651721, Length=454, Percent_Identity=29.9559471365639, Blast_Score=157, Evalue=8e-39,
Organism=Drosophila melanogaster, GI17864154, Length=454, Percent_Identity=29.9559471365639, Blast_Score=157, Evalue=8e-39,
Organism=Drosophila melanogaster, GI24652838, Length=444, Percent_Identity=29.0540540540541, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI17137572, Length=444, Percent_Identity=29.0540540540541, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI19922690, Length=309, Percent_Identity=32.0388349514563, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI45550900, Length=450, Percent_Identity=29.1111111111111, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17137380, Length=347, Percent_Identity=30.2593659942363, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24647048, Length=428, Percent_Identity=26.1682242990654, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24647046, Length=428, Percent_Identity=26.1682242990654, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI28572034, Length=166, Percent_Identity=37.9518072289157, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 44120; Mature: 43989

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQKFERKKPHVNVGTIGHVDHGKTTLTAAITMVLSKAGMAEAKGYDEIDKAPEEKARG
CCCCHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCHHCCCCCHHHCCHHHHHCC
ITINTTHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIVVFLNKADMVDDPELIELVEMEVRELLNEYEFPGDDTPIVVGSALKALE
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHH
CGCGKRECEWCGKIWQLMDIVDEYIPTPERDIDKPFLMPVEDVFTITGRGTVATGRVERG
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEECCCEEEECCEECC
KVKVGDEVEIIGLTTESRKTVVTGVEMFRKTMDEAQAGDNIGVLLRGVQRDEVERGQVLA
EEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCCEEE
KPGTIKPHTKFEAQVYVLTKEEGGRHTPFFNGYRPQFYFRTTDVTGVINLPDGVEMVMPG
CCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECC
DHVTIKVELITPIAMEEGLKFAIREGGRTVGAGVVSAIIE
CEEEEEEEEECCHHHHCCCEEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AKQKFERKKPHVNVGTIGHVDHGKTTLTAAITMVLSKAGMAEAKGYDEIDKAPEEKARG
CCCHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCHHCCCCCHHHCCHHHHHCC
ITINTTHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIVVFLNKADMVDDPELIELVEMEVRELLNEYEFPGDDTPIVVGSALKALE
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHH
CGCGKRECEWCGKIWQLMDIVDEYIPTPERDIDKPFLMPVEDVFTITGRGTVATGRVERG
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEECCCEEEECCEECC
KVKVGDEVEIIGLTTESRKTVVTGVEMFRKTMDEAQAGDNIGVLLRGVQRDEVERGQVLA
EEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCCEEE
KPGTIKPHTKFEAQVYVLTKEEGGRHTPFFNGYRPQFYFRTTDVTGVINLPDGVEMVMPG
CCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECC
DHVTIKVELITPIAMEEGLKFAIREGGRTVGAGVVSAIIE
CEEEEEEEEECCHHHHCCCEEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]