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The map label for this gene is ptsI [H]

Identifier: 167039481

GI number: 167039481

Start: 861130

End: 862851

Strand: Direct

Name: ptsI [H]

Synonym: Teth514_0827

Alternate gene names: 167039481

Gene position: 861130-862851 (Clockwise)

Preceding gene: 167039480

Following gene: 167039482

Centisome position: 35.04

GC content: 35.42

Gene sequence:

>1722_bases
ATGCTAAAAGGAGTTGCGGCATCCCCAGGGATAGCGATTGGGAAGGTTTTCTTATATACTAAAGAAAAAGCAGAAATCAA
CATGAAAAACATAGATGAATCAAAAGTAGAGTACGAAATTGAAAGGTTTAAGAAAGCTTTAGAAGTGACGAAGGAGCAAA
TACAAAAAATTAAAGAAGATGCATTGAAAGAATTTGGGAAGGATAAGGCAGAAATTTTTGAGGCACATTTAATGCTAGCA
AGTGACCCCGAACTTATTGAAGGGGTAGAAAACATGATAAAGACTGAACTTATAACTGCCGATAATGCGGTAAATAAAGT
AATTGAACAAAATGCTTCTGTAATGGAAAGTTTAAATGATGAATATTTAAAAGAAAGAGCTGTAGATTTAAGGGATGTAG
GAAGCCGTATAATAAATAATCTTTTAGGAGTAAAAAGCGTGAATCTTTCTGAACTTGATGAACAAGTGGTAGTTATAGCC
AAAGACTTAACCCCTTCAGATACAGCTACCATGAAAAAAGAAATGGTTTTAGGATTTGCTACAGATGTAGGCGGAAGAAC
TTCTCATACAGCTATAATGGCACGTTCCTTAGAAATTCCTGCTGTAGTAGGATTGGGTAATGTTACCTCTCAAGTTAATA
ATGGAGATACCATAATAGTAGATGGGTTGGAAGGGGTTGTCATTGTAAATCCTGACGAAAATACAATAAACGAATATAAG
GCTAAAAAAGAGAATTATGATAAAAAGGTGGAGAAATTAAAGAAATTAAAAGATTTGCCTGCAATAACTCCCGATGGCAA
AAAAGTCATGTTAGCGGCAAATATAGGAACTCCAAAAGATGTAAAAAGTGCGCTGGCCAATGGAGCAGAAGGGGTTGGAC
TTTTTAGAACTGAATTCTTATACATGGATAGAACTACTCTTCCTACAGAAGAAGAGCAATTTGAAGCCTACAAAGAAGTT
GCAGAAAAGATGGAAGGCCGGCCTGTTACTATAAGAACCTTAGATATAGGCGGAGATAAAGAGCTTCCTTATCTTGATAT
GCCTAAAGAGATGAATCCTTTTTTGGGATATAGGGCTATAAGGCTTTGCTTGGATAGGACAGACATATTTAAGACCCAAT
TACGAGCAATTTTAAGAGCAAGTGCTTATGGAAATATTCACATAATGTATCCTATGATATCTTCTATAGAAGATGTAAGA
AAAGCAAATATCGTTTTAAATGAAGTAAAAGCTGAGCTTGATAAAGAAGGCATAAAGTATGACAAAAACATAAAGGTAGG
TATAATGGTGGAAATACCTTCAGCTGCAGTGACTGCAGATATATTAGCTAAAGAAGTGGACTTCTTTAGCATAGGAACTA
ATGACCTGACGCAGTATACCCTTGCTGTTGACAGAATGAATGAACACGTAAAAGATTATTATCAGCCATTTAATCCAGCT
ATTTTAAGGCTTATAAAGTTTGTGATTGATGCGGCTCACAAAGAAGGAAAATTTGCAGCCATGTGCGGTGAAATGGCAGG
TGACCCTTTAGCTACAGTTATATTGTTAGGACTTGGCTTAGACGAATTTTCTATGAGTGCAACTTCTATACCTACAGTTA
AAAATATCATCAGAAATGTGGAATATGAAAGAACAAAAGAAATAGCGGAAAAGGTGCTAAATATTGCTGAGGCAGAAAAG
ATACAAAAAATGATGGAGGACATAATTAAAGACATTGAGTAA

Upstream 100 bases:

>100_bases
TTGCTTTATTTTTTTATTTTATTATTGTGCAAAAGTTTCACACTGGGACGAAAACTATGTTATAATAGACGTTAGAACTA
ATTTTAAGGAGGGTTAACAA

Downstream 100 bases:

>100_bases
ATCTCTGGCTTTTGCCAGAGATTTTTTATTAAAAAAATATCAAAAAAATAAAGGAAAATTTCAAAATGTATAGAATAATA
ATATTAAAGATTATTTGCGA

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MLKGVAASPGIAIGKVFLYTKEKAEINMKNIDESKVEYEIERFKKALEVTKEQIQKIKEDALKEFGKDKAEIFEAHLMLA
SDPELIEGVENMIKTELITADNAVNKVIEQNASVMESLNDEYLKERAVDLRDVGSRIINNLLGVKSVNLSELDEQVVVIA
KDLTPSDTATMKKEMVLGFATDVGGRTSHTAIMARSLEIPAVVGLGNVTSQVNNGDTIIVDGLEGVVIVNPDENTINEYK
AKKENYDKKVEKLKKLKDLPAITPDGKKVMLAANIGTPKDVKSALANGAEGVGLFRTEFLYMDRTTLPTEEEQFEAYKEV
AEKMEGRPVTIRTLDIGGDKELPYLDMPKEMNPFLGYRAIRLCLDRTDIFKTQLRAILRASAYGNIHIMYPMISSIEDVR
KANIVLNEVKAELDKEGIKYDKNIKVGIMVEIPSAAVTADILAKEVDFFSIGTNDLTQYTLAVDRMNEHVKDYYQPFNPA
ILRLIKFVIDAAHKEGKFAAMCGEMAGDPLATVILLGLGLDEFSMSATSIPTVKNIIRNVEYERTKEIAEKVLNIAEAEK
IQKMMEDIIKDIE

Sequences:

>Translated_573_residues
MLKGVAASPGIAIGKVFLYTKEKAEINMKNIDESKVEYEIERFKKALEVTKEQIQKIKEDALKEFGKDKAEIFEAHLMLA
SDPELIEGVENMIKTELITADNAVNKVIEQNASVMESLNDEYLKERAVDLRDVGSRIINNLLGVKSVNLSELDEQVVVIA
KDLTPSDTATMKKEMVLGFATDVGGRTSHTAIMARSLEIPAVVGLGNVTSQVNNGDTIIVDGLEGVVIVNPDENTINEYK
AKKENYDKKVEKLKKLKDLPAITPDGKKVMLAANIGTPKDVKSALANGAEGVGLFRTEFLYMDRTTLPTEEEQFEAYKEV
AEKMEGRPVTIRTLDIGGDKELPYLDMPKEMNPFLGYRAIRLCLDRTDIFKTQLRAILRASAYGNIHIMYPMISSIEDVR
KANIVLNEVKAELDKEGIKYDKNIKVGIMVEIPSAAVTADILAKEVDFFSIGTNDLTQYTLAVDRMNEHVKDYYQPFNPA
ILRLIKFVIDAAHKEGKFAAMCGEMAGDPLATVILLGLGLDEFSMSATSIPTVKNIIRNVEYERTKEIAEKVLNIAEAEK
IQKMMEDIIKDIE
>Mature_573_residues
MLKGVAASPGIAIGKVFLYTKEKAEINMKNIDESKVEYEIERFKKALEVTKEQIQKIKEDALKEFGKDKAEIFEAHLMLA
SDPELIEGVENMIKTELITADNAVNKVIEQNASVMESLNDEYLKERAVDLRDVGSRIINNLLGVKSVNLSELDEQVVVIA
KDLTPSDTATMKKEMVLGFATDVGGRTSHTAIMARSLEIPAVVGLGNVTSQVNNGDTIIVDGLEGVVIVNPDENTINEYK
AKKENYDKKVEKLKKLKDLPAITPDGKKVMLAANIGTPKDVKSALANGAEGVGLFRTEFLYMDRTTLPTEEEQFEAYKEV
AEKMEGRPVTIRTLDIGGDKELPYLDMPKEMNPFLGYRAIRLCLDRTDIFKTQLRAILRASAYGNIHIMYPMISSIEDVR
KANIVLNEVKAELDKEGIKYDKNIKVGIMVEIPSAAVTADILAKEVDFFSIGTNDLTQYTLAVDRMNEHVKDYYQPFNPA
ILRLIKFVIDAAHKEGKFAAMCGEMAGDPLATVILLGLGLDEFSMSATSIPTVKNIIRNVEYERTKEIAEKVLNIAEAEK
IQKMMEDIIKDIE

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=553, Percent_Identity=55.3345388788427, Blast_Score=615, Evalue=1e-177,
Organism=Escherichia coli, GI1788726, Length=566, Percent_Identity=35.8657243816254, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI48994992, Length=502, Percent_Identity=40.0398406374502, Blast_Score=355, Evalue=3e-99,
Organism=Escherichia coli, GI1789193, Length=577, Percent_Identity=33.6221837088388, Blast_Score=318, Evalue=7e-88,
Organism=Escherichia coli, GI1787994, Length=450, Percent_Identity=28.8888888888889, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI226510935, Length=208, Percent_Identity=26.4423076923077, Blast_Score=87, Evalue=4e-18,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63766; Mature: 63766

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKGVAASPGIAIGKVFLYTKEKAEINMKNIDESKVEYEIERFKKALEVTKEQIQKIKED
CCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALKEFGKDKAEIFEAHLMLASDPELIEGVENMIKTELITADNAVNKVIEQNASVMESLND
HHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYLKERAVDLRDVGSRIINNLLGVKSVNLSELDEQVVVIAKDLTPSDTATMKKEMVLGFA
HHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHCCEEEEEEECCCCCHHHHHHHHHHHEEE
TDVGGRTSHTAIMARSLEIPAVVGLGNVTSQVNNGDTIIVDGLEGVVIVNPDENTINEYK
CCCCCCCCHHHHHHHCCCCCEEEECCCHHHCCCCCCEEEEECCCEEEEECCCCCHHHHHH
AKKENYDKKVEKLKKLKDLPAITPDGKKVMLAANIGTPKDVKSALANGAEGVGLFRTEFL
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHEE
YMDRTTLPTEEEQFEAYKEVAEKMEGRPVTIRTLDIGGDKELPYLDMPKEMNPFLGYRAI
EECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHH
RLCLDRTDIFKTQLRAILRASAYGNIHIMYPMISSIEDVRKANIVLNEVKAELDKEGIKY
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DKNIKVGIMVEIPSAAVTADILAKEVDFFSIGTNDLTQYTLAVDRMNEHVKDYYQPFNPA
CCCCEEEEEEECCCHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
ILRLIKFVIDAAHKEGKFAAMCGEMAGDPLATVILLGLGLDEFSMSATSIPTVKNIIRNV
HHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHCC
EYERTKEIAEKVLNIAEAEKIQKMMEDIIKDIE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKGVAASPGIAIGKVFLYTKEKAEINMKNIDESKVEYEIERFKKALEVTKEQIQKIKED
CCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALKEFGKDKAEIFEAHLMLASDPELIEGVENMIKTELITADNAVNKVIEQNASVMESLND
HHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYLKERAVDLRDVGSRIINNLLGVKSVNLSELDEQVVVIAKDLTPSDTATMKKEMVLGFA
HHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHCCEEEEEEECCCCCHHHHHHHHHHHEEE
TDVGGRTSHTAIMARSLEIPAVVGLGNVTSQVNNGDTIIVDGLEGVVIVNPDENTINEYK
CCCCCCCCHHHHHHHCCCCCEEEECCCHHHCCCCCCEEEEECCCEEEEECCCCCHHHHHH
AKKENYDKKVEKLKKLKDLPAITPDGKKVMLAANIGTPKDVKSALANGAEGVGLFRTEFL
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHEE
YMDRTTLPTEEEQFEAYKEVAEKMEGRPVTIRTLDIGGDKELPYLDMPKEMNPFLGYRAI
EECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHH
RLCLDRTDIFKTQLRAILRASAYGNIHIMYPMISSIEDVRKANIVLNEVKAELDKEGIKY
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DKNIKVGIMVEIPSAAVTADILAKEVDFFSIGTNDLTQYTLAVDRMNEHVKDYYQPFNPA
CCCCEEEEEEECCCHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
ILRLIKFVIDAAHKEGKFAAMCGEMAGDPLATVILLGLGLDEFSMSATSIPTVKNIIRNV
HHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHCC
EYERTKEIAEKVLNIAEAEKIQKMMEDIIKDIE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]