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The map label for this gene is acoA [H]

Identifier: 167039404

GI number: 167039404

Start: 773683

End: 774471

Strand: Reverse

Name: acoA [H]

Synonym: Teth514_0748

Alternate gene names: 167039404

Gene position: 774471-773683 (Counterclockwise)

Preceding gene: 167039406

Following gene: 167039403

Centisome position: 31.52

GC content: 40.05

Gene sequence:

>789_bases
ATGAACATTCCTAAAGAAACTCTTATACGTATGTATCTTGAGATGGTTACAATTCGATTATATGAGGAAACTATGGCTGA
AGCCTATCAGGAGGGGAAATACCCAGTATTCAACATTGCATCAGGCCCAGTCCCTGGTGAGATGCATTTAGCTGCGGGAC
AGGAGCCGGTAGCTGTAGGAGTATGCATGCACCTTAAAAAGGAGGATGCTGTAGTCGGTACTCACCGGCCTCACCACTTT
GCTATTGCAAAAGGAGTAGATCTCAAACGTATGACAGCAGAAATATTTGGTAAAGTTACCGGTTTAGGACGCGGCAAAGG
TGGTCACATGCACCTCTTTGACCCTGATGTACACTTCAGCTGTAGCGGCATCGTCGGTGCTAGCATTCCCCAAGCAGTAG
GAGCAGCCCTAACTTTCAAAATGCGCAAGGAAAAACGTGTAGCTGTGGCCTTTTTTGGTGAAATTTATGCTATGAAGGAC
GTAATTATAACCTCAGAAGCAGGCTGTTATAACACAAAAATTTCTACAAATGGTTCAGTTTCCATTAATGGTAAGGTTAA
AATGAGCACGATCGAATTTGACAAAAATATCTTTGTAAAAGAAGCCGGATCGCACGGCGTCGGCAGTCACGTATTATTAA
AGGGAAGTAAAAATTCAATAGTAAAAATCCTTTACGGCTATGAAGGAGTCGAATTATACTTTGATAAAATAGGCTACAAA
TTAAAAAATGGTGAAAAAATAAAATTATATTTAGACAAAGATGAAAAAGTCGTTGAAGACATTATTTAA

Upstream 100 bases:

>100_bases
ACAAAAAAAATTAAAACATATTCACCTTCTTTGACATTATAAAGCAGAGTAGTATATTGTGAAAAATTGAAAAATTGTAA
ATGGATAGGAGTGAAAAGGT

Downstream 100 bases:

>100_bases
TTTGTTTCAGTTGGCTTTTGAAAAATTCACCGTGGGAATGGGAGCCTTTCCCATGAATCTGTTTGATTTTGAAGAATATA
TCATTTCTGTCCGGCATATC

Product: dehydrogenase, E1 component

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MNIPKETLIRMYLEMVTIRLYEETMAEAYQEGKYPVFNIASGPVPGEMHLAAGQEPVAVGVCMHLKKEDAVVGTHRPHHF
AIAKGVDLKRMTAEIFGKVTGLGRGKGGHMHLFDPDVHFSCSGIVGASIPQAVGAALTFKMRKEKRVAVAFFGEIYAMKD
VIITSEAGCYNTKISTNGSVSINGKVKMSTIEFDKNIFVKEAGSHGVGSHVLLKGSKNSIVKILYGYEGVELYFDKIGYK
LKNGEKIKLYLDKDEKVVEDII

Sequences:

>Translated_262_residues
MNIPKETLIRMYLEMVTIRLYEETMAEAYQEGKYPVFNIASGPVPGEMHLAAGQEPVAVGVCMHLKKEDAVVGTHRPHHF
AIAKGVDLKRMTAEIFGKVTGLGRGKGGHMHLFDPDVHFSCSGIVGASIPQAVGAALTFKMRKEKRVAVAFFGEIYAMKD
VIITSEAGCYNTKISTNGSVSINGKVKMSTIEFDKNIFVKEAGSHGVGSHVLLKGSKNSIVKILYGYEGVELYFDKIGYK
LKNGEKIKLYLDKDEKVVEDII
>Mature_262_residues
MNIPKETLIRMYLEMVTIRLYEETMAEAYQEGKYPVFNIASGPVPGEMHLAAGQEPVAVGVCMHLKKEDAVVGTHRPHHF
AIAKGVDLKRMTAEIFGKVTGLGRGKGGHMHLFDPDVHFSCSGIVGASIPQAVGAALTFKMRKEKRVAVAFFGEIYAMKD
VIITSEAGCYNTKISTNGSVSINGKVKMSTIEFDKNIFVKEAGSHGVGSHVLLKGSKNSIVKILYGYEGVELYFDKIGYK
LKNGEKIKLYLDKDEKVVEDII

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=146, Percent_Identity=28.0821917808219, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17536047, Length=154, Percent_Identity=32.4675324675325, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI32564172, Length=154, Percent_Identity=32.4675324675325, Blast_Score=84, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6321026, Length=150, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24639748, Length=211, Percent_Identity=31.2796208530806, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24639740, Length=152, Percent_Identity=30.2631578947368, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24639744, Length=152, Percent_Identity=30.2631578947368, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI28571106, Length=152, Percent_Identity=30.2631578947368, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24639746, Length=140, Percent_Identity=31.4285714285714, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 28775; Mature: 28775

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIPKETLIRMYLEMVTIRLYEETMAEAYQEGKYPVFNIASGPVPGEMHLAAGQEPVAVG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEECCCCCEEEE
VCMHLKKEDAVVGTHRPHHFAIAKGVDLKRMTAEIFGKVTGLGRGKGGHMHLFDPDVHFS
EEEEECCCCCEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEE
CSGIVGASIPQAVGAALTFKMRKEKRVAVAFFGEIYAMKDVIITSEAGCYNTKISTNGSV
ECCCCCCCCHHHHHHHEEEEECCCCEEEEEEHHHHHEEEEEEEECCCCCEEEEECCCCEE
SINGKVKMSTIEFDKNIFVKEAGSHGVGSHVLLKGSKNSIVKILYGYEGVELYFDKIGYK
EECCEEEEEEEEECCCEEEEECCCCCCCCEEEEECCCCCEEEEEECCCCCEEEEEHHCEE
LKNGEKIKLYLDKDEKVVEDII
ECCCCEEEEEECCCHHHHHHCC
>Mature Secondary Structure
MNIPKETLIRMYLEMVTIRLYEETMAEAYQEGKYPVFNIASGPVPGEMHLAAGQEPVAVG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEECCCCCEEEE
VCMHLKKEDAVVGTHRPHHFAIAKGVDLKRMTAEIFGKVTGLGRGKGGHMHLFDPDVHFS
EEEEECCCCCEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEE
CSGIVGASIPQAVGAALTFKMRKEKRVAVAFFGEIYAMKDVIITSEAGCYNTKISTNGSV
ECCCCCCCCHHHHHHHEEEEECCCCEEEEEEHHHHHEEEEEEEECCCCCEEEEECCCCEE
SINGKVKMSTIEFDKNIFVKEAGSHGVGSHVLLKGSKNSIVKILYGYEGVELYFDKIGYK
EECCEEEEEEEEECCCEEEEECCCCCCCCEEEEECCCCCEEEEEECCCCCEEEEEHHCEE
LKNGEKIKLYLDKDEKVVEDII
ECCCCEEEEEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]