Click here to switch to the map view.

The map label for this gene is mnmE [H]

Identifier: 167039341

GI number: 167039341

Start: 716560

End: 717786

Strand: Direct

Name: mnmE [H]

Synonym: Teth514_0683

Alternate gene names: 167039341

Gene position: 716560-717786 (Clockwise)

Preceding gene: 167039340

Following gene: 167039344

Centisome position: 29.16

GC content: 36.19

Gene sequence:

>1227_bases
ATGAACACGACACCTATTTCTTCCAGGCTCCACATAGCTATTTTTGGCAGGAGAAATGCAGGTAAATCAAGCCTTATAAA
TGCTCTTACAAACCAGGAAGTAGCCCTTGTATCTGATGTTGCAGGTACTACTACTGATCCTGTGTCGAAGGCAATGGAAA
TACTTCCAATAGGACCAGTTGTAATCATCGATACTGCTGGACTTGATGATACGGGAGAGTTAGGGGAACTTAGGGTAAAA
AAAACCTATGAAGTTTTAAACAGAACAGACCTTGCAATTCTTGTCATAGATGGCACTATAGGGCTTTCAGAGTTCGAAGA
AAATGTCTTGAAAATCATTAGAGATAAAAATATTCCAGTTGTAGGGGTAATAAATAAAAAAGATTTGTCACAATATTCTG
AGGAAGATAAGAGAAAATGGGAAGAAAAATTAAAACTAGAGCTTATTGAAGTTTCTGCTTTAAAAAAACATGGAATTGAA
GCCCTAAAAATGATGTTAATAAAGAAAGCACCATATGACGACAGGGAACTTTCGATTGTAGGTGATCTAATAAAACCGGG
AGACTTTGTAGTTCTTGTTATTCCTATAGATAAAGCTGCTCCAAAAGGAAGACTTATACTTCCGCAGCAACAGACAATAA
GAGATATTCTTGACAATGATGCGATGGCTATTGTTACAAAAGAGCATGAGCTAAAAGAGACACTTCAAAATCTAGGGAAA
AAGCCATCATTGGTTATTACAGATTCTCAGGCTTTTTTGAAAGTTAGCGCTGATACGCCCAAAGATATACCGCTTACATC
TTTTTCTATTCTTTTTGCACGATATAAAGGTGATTTGGAGGAACTTGTAAGAGGTGTAAAAGCAATAAAAAAACTAAAAC
CTGGTGATAAAGTCCTCATTGCTGAAGGATGTACACATCACAGGCAGTCTGATGATATAGGAAAAGTAAAAATACCCAGA
TGGATACGCCAGATTGTTGGAGGAGACATACAATTTGACTGGTCAAGTGGCATAACTTTTCCAGACAATCTCGAAAAATA
TAGCTTGATTGTACATTGTGGAGCATGTATGTTGAACAGAAGGGAAATGATGTATAGAATTTCATATGCAAAAAGCAAAA
ACATTCCAATAGTAAACTATGGGGTATTAATAGCTTATGTTCAAGGCTTAATGCCGAGAGCTATAGAAATGTTTCCACTG
GCAAAAATGATTTATGAAGAAGAATAG

Upstream 100 bases:

>100_bases
GACTCTTTACAGAGGAAGAACTTGATATAATATTTAACCCTTATGAAATGACAAAACCAGGAATACCAGGGAAAAGAAGA
TTGAAAGGAGAAAAATAATT

Downstream 100 bases:

>100_bases
AATGGGACAATTATACCTTTGTCCCATAAACTTTTTTATATTGAAACACTAAAACATAAAGTGTATACGATAGTGCGATT
ATTCCACTTACTATAAAAGT

Product: small GTP-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MNTTPISSRLHIAIFGRRNAGKSSLINALTNQEVALVSDVAGTTTDPVSKAMEILPIGPVVIIDTAGLDDTGELGELRVK
KTYEVLNRTDLAILVIDGTIGLSEFEENVLKIIRDKNIPVVGVINKKDLSQYSEEDKRKWEEKLKLELIEVSALKKHGIE
ALKMMLIKKAPYDDRELSIVGDLIKPGDFVVLVIPIDKAAPKGRLILPQQQTIRDILDNDAMAIVTKEHELKETLQNLGK
KPSLVITDSQAFLKVSADTPKDIPLTSFSILFARYKGDLEELVRGVKAIKKLKPGDKVLIAEGCTHHRQSDDIGKVKIPR
WIRQIVGGDIQFDWSSGITFPDNLEKYSLIVHCGACMLNRREMMYRISYAKSKNIPIVNYGVLIAYVQGLMPRAIEMFPL
AKMIYEEE

Sequences:

>Translated_408_residues
MNTTPISSRLHIAIFGRRNAGKSSLINALTNQEVALVSDVAGTTTDPVSKAMEILPIGPVVIIDTAGLDDTGELGELRVK
KTYEVLNRTDLAILVIDGTIGLSEFEENVLKIIRDKNIPVVGVINKKDLSQYSEEDKRKWEEKLKLELIEVSALKKHGIE
ALKMMLIKKAPYDDRELSIVGDLIKPGDFVVLVIPIDKAAPKGRLILPQQQTIRDILDNDAMAIVTKEHELKETLQNLGK
KPSLVITDSQAFLKVSADTPKDIPLTSFSILFARYKGDLEELVRGVKAIKKLKPGDKVLIAEGCTHHRQSDDIGKVKIPR
WIRQIVGGDIQFDWSSGITFPDNLEKYSLIVHCGACMLNRREMMYRISYAKSKNIPIVNYGVLIAYVQGLMPRAIEMFPL
AKMIYEEE
>Mature_408_residues
MNTTPISSRLHIAIFGRRNAGKSSLINALTNQEVALVSDVAGTTTDPVSKAMEILPIGPVVIIDTAGLDDTGELGELRVK
KTYEVLNRTDLAILVIDGTIGLSEFEENVLKIIRDKNIPVVGVINKKDLSQYSEEDKRKWEEKLKLELIEVSALKKHGIE
ALKMMLIKKAPYDDRELSIVGDLIKPGDFVVLVIPIDKAAPKGRLILPQQQTIRDILDNDAMAIVTKEHELKETLQNLGK
KPSLVITDSQAFLKVSADTPKDIPLTSFSILFARYKGDLEELVRGVKAIKKLKPGDKVLIAEGCTHHRQSDDIGKVKIPR
WIRQIVGGDIQFDWSSGITFPDNLEKYSLIVHCGACMLNRREMMYRISYAKSKNIPIVNYGVLIAYVQGLMPRAIEMFPL
AKMIYEEE

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG1160

COG function: function code R; Predicted GTPases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Escherichia coli, GI2367268, Length=169, Percent_Identity=30.7692307692308, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082120, Length=162, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323665, Length=172, Percent_Identity=33.7209302325581, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 45553; Mature: 45553

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTPISSRLHIAIFGRRNAGKSSLINALTNQEVALVSDVAGTTTDPVSKAMEILPIGPV
CCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCCCE
VIIDTAGLDDTGELGELRVKKTYEVLNRTDLAILVIDGTIGLSEFEENVLKIIRDKNIPV
EEEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCE
VGVINKKDLSQYSEEDKRKWEEKLKLELIEVSALKKHGIEALKMMLIKKAPYDDRELSIV
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
GDLIKPGDFVVLVIPIDKAAPKGRLILPQQQTIRDILDNDAMAIVTKEHELKETLQNLGK
ECCCCCCCEEEEEEEECCCCCCCCEECCCHHHHHHHHCCCCEEEEECHHHHHHHHHHHCC
KPSLVITDSQAFLKVSADTPKDIPLTSFSILFARYKGDLEELVRGVKAIKKLKPGDKVLI
CCCEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEE
AEGCTHHRQSDDIGKVKIPRWIRQIVGGDIQFDWSSGITFPDNLEKYSLIVHCGACMLNR
ECCCCCCCCCCCCCCEEHHHHHHHHHCCCEEEECCCCCCCCCCCCCEEEEEEHHHHHHHH
REMMYRISYAKSKNIPIVNYGVLIAYVQGLMPRAIEMFPLAKMIYEEE
HHHHHEEHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNTTPISSRLHIAIFGRRNAGKSSLINALTNQEVALVSDVAGTTTDPVSKAMEILPIGPV
CCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCCCE
VIIDTAGLDDTGELGELRVKKTYEVLNRTDLAILVIDGTIGLSEFEENVLKIIRDKNIPV
EEEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCE
VGVINKKDLSQYSEEDKRKWEEKLKLELIEVSALKKHGIEALKMMLIKKAPYDDRELSIV
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
GDLIKPGDFVVLVIPIDKAAPKGRLILPQQQTIRDILDNDAMAIVTKEHELKETLQNLGK
ECCCCCCCEEEEEEEECCCCCCCCEECCCHHHHHHHHCCCCEEEEECHHHHHHHHHHHCC
KPSLVITDSQAFLKVSADTPKDIPLTSFSILFARYKGDLEELVRGVKAIKKLKPGDKVLI
CCCEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEE
AEGCTHHRQSDDIGKVKIPRWIRQIVGGDIQFDWSSGITFPDNLEKYSLIVHCGACMLNR
ECCCCCCCCCCCCCCEEHHHHHHHHHCCCEEEECCCCCCCCCCCCCEEEEEEHHHHHHHH
REMMYRISYAKSKNIPIVNYGVLIAYVQGLMPRAIEMFPLAKMIYEEE
HHHHHEEHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]