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The map label for this gene is aspA [H]

Identifier: 167039340

GI number: 167039340

Start: 715139

End: 716557

Strand: Direct

Name: aspA [H]

Synonym: Teth514_0682

Alternate gene names: 167039340

Gene position: 715139-716557 (Clockwise)

Preceding gene: 167039339

Following gene: 167039341

Centisome position: 29.1

GC content: 36.79

Gene sequence:

>1419_bases
ATGAGATATAGAATTGAGCATGATTTGCTAGGGCAAAAAGAGGTTCCAGTTGATGCTTATTATGGCATACATACATTAAG
AGCTTATGAAAATTTTAATATATCAGGACAAAAAATTCATAGAGAACTTATTATATCGCTTGCGATGGTCAAAAAGGCTG
CAGCAATTGCAAATATGAAAGCTGGTTTGCTTGATGAAAAAAGAGCGAAGGCAATAATTTTAGCTTGTGATGAGATAATA
TCAGGTAAATACCATGATCAATTTATTGTTGATGCCCTCCAGGGAGGTGCAGGTACTTCTTCTAATATGAATGCTAACGA
AGTTATTGCAAATAGGGCAATAGAACTACTGGGGGGTAGTAAAGGGGACTATTCTATTGTACACCCACTGGAACATGTAA
ACTTGAGTCAATCTACAAATGATGTTTTCCCGACAGCGGTGAGAATTGCAGCAATACGTCTTTTAAAGCCTTTGAGTGAA
GATTTAGCTGAGCTTCAAAGTGTGCTTCAAGAAAAAGAAGAAGAGTTTTCTGATGTATGTAAAATGGGACGAACCGAGTT
ACAGGATGCTCTTCCTGTTATGCTTGGTCAGGAATTTGGTGCATATGCACAAGCAGTTGCAAGGGACAGATGGAGGATAT
ATAAAGTTGAAGAAAGGTTGAGACAGGTAAACATTGGCGGTACTGCTGTTGGGACAGGAGTAAATGCCGAGCTAAAGTAT
ATATTCTCAGTTATAGAAATATTAAGAGAAATAACAGGACTAGGACTTGCAAGAGCGGAATATATGATGGATCCTACTCA
GAACAATGATGTTTTTGTTGAGGTATCTGGGCTTTTAAAAGCGGCAGCCGTTAACCTTATAAAAATTTCGAATGATATAA
GGCTTATGTCGTCAGGACCTAAATGTGGGCTTATGGAAATTAATATACCAGAGGTACAGTCAGGTTCTTCGATTATGCCT
GGAAAAGTGAATCCTGTTATACCTGAGGCTGTAAAGCAGGCAGCGTTTCAAGTTATAGCATGTGACCATGCAATAACTTT
GTGTGCCCAAGCAGGTGAATTTGAGCTTAATTTCACATTACCTCAAATTGCATATAATCTTTTTATGCAGATAGGTCTAT
TAAGAAATGCTATACGAATATTTATAGATAAATGCATTAAGGGAATAACTGCGAATAAAGATTACTTAAAAGAAACTATT
GAAAAAAGTTATTCGTATGCAACAGCGCTAGTTCCTTACATTGGGTATGAAAAGGCAACGGAAATTGCAAAAATGGCTAA
AGAAAAAGGAAAAACGATAAGGGAAGTTGTGTTAGAGGCTGGACTCTTTACAGAGGAAGAACTTGATATAATATTTAACC
CTTATGAAATGACAAAACCAGGAATACCAGGGAAAAGAAGATTGAAAGGAGAAAAATAA

Upstream 100 bases:

>100_bases
TGTAAGTTTGACTATTAAATATCGATAAATTGTTGACAATCTGACGTTTCTGTATTATTGATTATATTGATGATTTATAT
ATAAAGTGGAGGCAATTTTT

Downstream 100 bases:

>100_bases
TTATGAACACGACACCTATTTCTTCCAGGCTCCACATAGCTATTTTTGGCAGGAGAAATGCAGGTAAATCAAGCCTTATA
AATGCTCTTACAAACCAGGA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MRYRIEHDLLGQKEVPVDAYYGIHTLRAYENFNISGQKIHRELIISLAMVKKAAAIANMKAGLLDEKRAKAIILACDEII
SGKYHDQFIVDALQGGAGTSSNMNANEVIANRAIELLGGSKGDYSIVHPLEHVNLSQSTNDVFPTAVRIAAIRLLKPLSE
DLAELQSVLQEKEEEFSDVCKMGRTELQDALPVMLGQEFGAYAQAVARDRWRIYKVEERLRQVNIGGTAVGTGVNAELKY
IFSVIEILREITGLGLARAEYMMDPTQNNDVFVEVSGLLKAAAVNLIKISNDIRLMSSGPKCGLMEINIPEVQSGSSIMP
GKVNPVIPEAVKQAAFQVIACDHAITLCAQAGEFELNFTLPQIAYNLFMQIGLLRNAIRIFIDKCIKGITANKDYLKETI
EKSYSYATALVPYIGYEKATEIAKMAKEKGKTIREVVLEAGLFTEEELDIIFNPYEMTKPGIPGKRRLKGEK

Sequences:

>Translated_472_residues
MRYRIEHDLLGQKEVPVDAYYGIHTLRAYENFNISGQKIHRELIISLAMVKKAAAIANMKAGLLDEKRAKAIILACDEII
SGKYHDQFIVDALQGGAGTSSNMNANEVIANRAIELLGGSKGDYSIVHPLEHVNLSQSTNDVFPTAVRIAAIRLLKPLSE
DLAELQSVLQEKEEEFSDVCKMGRTELQDALPVMLGQEFGAYAQAVARDRWRIYKVEERLRQVNIGGTAVGTGVNAELKY
IFSVIEILREITGLGLARAEYMMDPTQNNDVFVEVSGLLKAAAVNLIKISNDIRLMSSGPKCGLMEINIPEVQSGSSIMP
GKVNPVIPEAVKQAAFQVIACDHAITLCAQAGEFELNFTLPQIAYNLFMQIGLLRNAIRIFIDKCIKGITANKDYLKETI
EKSYSYATALVPYIGYEKATEIAKMAKEKGKTIREVVLEAGLFTEEELDIIFNPYEMTKPGIPGKRRLKGEK
>Mature_472_residues
MRYRIEHDLLGQKEVPVDAYYGIHTLRAYENFNISGQKIHRELIISLAMVKKAAAIANMKAGLLDEKRAKAIILACDEII
SGKYHDQFIVDALQGGAGTSSNMNANEVIANRAIELLGGSKGDYSIVHPLEHVNLSQSTNDVFPTAVRIAAIRLLKPLSE
DLAELQSVLQEKEEEFSDVCKMGRTELQDALPVMLGQEFGAYAQAVARDRWRIYKVEERLRQVNIGGTAVGTGVNAELKY
IFSVIEILREITGLGLARAEYMMDPTQNNDVFVEVSGLLKAAAVNLIKISNDIRLMSSGPKCGLMEINIPEVQSGSSIMP
GKVNPVIPEAVKQAAFQVIACDHAITLCAQAGEFELNFTLPQIAYNLFMQIGLLRNAIRIFIDKCIKGITANKDYLKETI
EKSYSYATALVPYIGYEKATEIAKMAKEKGKTIREVVLEAGLFTEEELDIIFNPYEMTKPGIPGKRRLKGEK

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=461, Percent_Identity=42.5162689804772, Blast_Score=357, Evalue=2e-98,
Organism=Escherichia coli, GI87082375, Length=466, Percent_Identity=47.8540772532189, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1787896, Length=455, Percent_Identity=41.978021978022, Blast_Score=344, Evalue=6e-96,
Organism=Escherichia coli, GI1790398, Length=298, Percent_Identity=27.8523489932886, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17553882, Length=462, Percent_Identity=43.2900432900433, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=41.5902140672783, Blast_Score=254, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6324993, Length=461, Percent_Identity=43.3839479392625, Blast_Score=362, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24640179, Length=462, Percent_Identity=42.8571428571429, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24640177, Length=462, Percent_Identity=42.8571428571429, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24662684, Length=462, Percent_Identity=43.7229437229437, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=41.2526997840173, Blast_Score=345, Evalue=4e-95,
Organism=Drosophila melanogaster, GI24583245, Length=462, Percent_Identity=39.3939393939394, Blast_Score=328, Evalue=4e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 52217; Mature: 52217

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYRIEHDLLGQKEVPVDAYYGIHTLRAYENFNISGQKIHRELIISLAMVKKAAAIANMK
CCEECCHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AGLLDEKRAKAIILACDEIISGKYHDQFIVDALQGGAGTSSNMNANEVIANRAIELLGGS
HCCCCHHHHCEEEEEEHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCC
KGDYSIVHPLEHVNLSQSTNDVFPTAVRIAAIRLLKPLSEDLAELQSVLQEKEEEFSDVC
CCCCEEECCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMGRTELQDALPVMLGQEFGAYAQAVARDRWRIYKVEERLRQVNIGGTAVGTGVNAELKY
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHH
IFSVIEILREITGLGLARAEYMMDPTQNNDVFVEVSGLLKAAAVNLIKISNDIRLMSSGP
HHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHEEECCCEEEEECCC
KCGLMEINIPEVQSGSSIMPGKVNPVIPEAVKQAAFQVIACDHAITLCAQAGEFELNFTL
CCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEH
PQIAYNLFMQIGLLRNAIRIFIDKCIKGITANKDYLKETIEKSYSYATALVPYIGYEKAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
EIAKMAKEKGKTIREVVLEAGLFTEEELDIIFNPYEMTKPGIPGKRRLKGEK
HHHHHHHHHCHHHHHHHHHHCCCCCCCCCEEECCHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRYRIEHDLLGQKEVPVDAYYGIHTLRAYENFNISGQKIHRELIISLAMVKKAAAIANMK
CCEECCHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AGLLDEKRAKAIILACDEIISGKYHDQFIVDALQGGAGTSSNMNANEVIANRAIELLGGS
HCCCCHHHHCEEEEEEHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCC
KGDYSIVHPLEHVNLSQSTNDVFPTAVRIAAIRLLKPLSEDLAELQSVLQEKEEEFSDVC
CCCCEEECCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMGRTELQDALPVMLGQEFGAYAQAVARDRWRIYKVEERLRQVNIGGTAVGTGVNAELKY
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHH
IFSVIEILREITGLGLARAEYMMDPTQNNDVFVEVSGLLKAAAVNLIKISNDIRLMSSGP
HHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHEEECCCEEEEECCC
KCGLMEINIPEVQSGSSIMPGKVNPVIPEAVKQAAFQVIACDHAITLCAQAGEFELNFTL
CCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEH
PQIAYNLFMQIGLLRNAIRIFIDKCIKGITANKDYLKETIEKSYSYATALVPYIGYEKAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
EIAKMAKEKGKTIREVVLEAGLFTEEELDIIFNPYEMTKPGIPGKRRLKGEK
HHHHHHHHHCHHHHHHHHHHCCCCCCCCCEEECCHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]