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The map label for this gene is gatA [H]

Identifier: 167039199

GI number: 167039199

Start: 559037

End: 560503

Strand: Direct

Name: gatA [H]

Synonym: Teth514_0540

Alternate gene names: 167039199

Gene position: 559037-560503 (Clockwise)

Preceding gene: 167039198

Following gene: 167039200

Centisome position: 22.75

GC content: 37.9

Gene sequence:

>1467_bases
ATGGAATTATATAGTTTGACAATTCACGAACTTAGAGAACTTCTCAAAAAGAGAGAAGTCAGCGCTTTGGAAGTGACAAA
ATCCTATCTTGAAAGAATAAAAGAAGTAGAACCTAAAATAGATGCTCTTGTCACAATTACAGAGGATTTTGCCTTGCAAA
AAGCAAAAGAGGCAGATGAGAAAATCAAAAAGGGAGAGGACACGGCCCTTACAGGCATTCCTGTCATAATAAAGGACAAC
ATTTCTACTGAGGGAATAAAAACCACTTGTTCTTCTAAGATGCTAGAAAACTATATTCCTCCCTATAATGCTACAGTAGT
GGAAAAATTACTAGAAGAAGGAGTGATAATTTTAGGAAAATCCAATTTAGACGAATTTGCAATGGGGTCTTCTACAGAAA
ATTCCGCTTTTAAAACTACAAAAAATCCTTGGGACTTATCCCGTGTTCCAGGAGGGTCTTCTGGTGGTTCTGCAGCAGCT
ATAGCGGCAGACGAGGCGGCTTTTGCTTTGGGTTCAGATACAGGTGGTTCTATAAGACAACCAGCTTCTTTATGCGGTGT
AGTAGGGATGAAACCTACTTATGGATTGGTGTCAAGATATGGCCTTGTAGCTTTTGCCTCTTCACTTGACCAGATTGGAC
CCTTTACAAAAGATGTTACAGACTGTGCTATTGTATTAAATACAATTATAGGGCATGACCCAAAAGACTCTACTTCTTTA
AAAATAGACAAGCCTGATTACACTTCTTATCTCAAAGAAGATATTAAAGGTTTGAGGATAGGAGTGGCTAAAGAATTTTT
TGGCGAGGGAATAGAAGAAGGGGTAAAAGAAACTGTACAAGAATCCATAAAAGTTTTACAGGATTTAGGAGCAGAAATCA
TAGATATATCTATTCCTTATGTAGAATATGCTCTTCCAGCTTATTATATAATCGCTTCGGCAGAGGCTAGTTCCAACCTT
GCAAGGTATGATGGCATAAGATACGGCCATATTGCAGAAAAATATGAAGATTTGATTGACATGTACATGGTTACGAGAAG
CGAAGGATTTGGCAAAGAAGTAAAAAGAAGGATAATGCTAGGGACTTATGCTTTAAGCTCTGGCTATTATGATGCTTATT
ACAAAAAAGCATTAAAGGTCAGAACTCTCATTAAAAATGACTTTGAAAAAGCTTTTGAAAAATGCGATGTAATAATAGGT
CCAACAAGTCCTACTGTAGCTTTTAAAATTGGAGAAAGGGCAAACGACCCGTTAGCCATGTACTTAGCAGATATATACAC
TGTATCGGTTAACATAGCGGGGCTCCCTGGCATATCCATACCTTGTGGTCTTTCAGATGGCTTACCTGTTGGACTTCAGA
TAATTGGCAGACATTTTGACGAAGGGAAAATATTAAATGTCGCTTATGCCTTTGAACAAGCCAATAAATTTAGTGCAAAA
CCACAGGCCATAGGAGGTGCAAGATAA

Upstream 100 bases:

>100_bases
AAAGCCTTCAATGGACAGAGACAAAATATTGATGAATGCACCTTATAAAGAGAACGGCTGTTTTAAAGTGCCAAAGATTA
TAGAATGAGGAGGTACATAA

Downstream 100 bases:

>100_bases
TGAAGTACGAAGCAGTGATTGGCTTAGAGGTCCATGCCGAACTTTTGACAGAGAGCAAAATCTTCTGTGGTTGTACTACC
AAATTTGGCGGTGAACCTAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MELYSLTIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALVTITEDFALQKAKEADEKIKKGEDTALTGIPVIIKDN
ISTEGIKTTCSSKMLENYIPPYNATVVEKLLEEGVIILGKSNLDEFAMGSSTENSAFKTTKNPWDLSRVPGGSSGGSAAA
IAADEAAFALGSDTGGSIRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIGHDPKDSTSL
KIDKPDYTSYLKEDIKGLRIGVAKEFFGEGIEEGVKETVQESIKVLQDLGAEIIDISIPYVEYALPAYYIIASAEASSNL
ARYDGIRYGHIAEKYEDLIDMYMVTRSEGFGKEVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFEKAFEKCDVIIG
PTSPTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIPCGLSDGLPVGLQIIGRHFDEGKILNVAYAFEQANKFSAK
PQAIGGAR

Sequences:

>Translated_488_residues
MELYSLTIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALVTITEDFALQKAKEADEKIKKGEDTALTGIPVIIKDN
ISTEGIKTTCSSKMLENYIPPYNATVVEKLLEEGVIILGKSNLDEFAMGSSTENSAFKTTKNPWDLSRVPGGSSGGSAAA
IAADEAAFALGSDTGGSIRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIGHDPKDSTSL
KIDKPDYTSYLKEDIKGLRIGVAKEFFGEGIEEGVKETVQESIKVLQDLGAEIIDISIPYVEYALPAYYIIASAEASSNL
ARYDGIRYGHIAEKYEDLIDMYMVTRSEGFGKEVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFEKAFEKCDVIIG
PTSPTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIPCGLSDGLPVGLQIIGRHFDEGKILNVAYAFEQANKFSAK
PQAIGGAR
>Mature_488_residues
MELYSLTIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALVTITEDFALQKAKEADEKIKKGEDTALTGIPVIIKDN
ISTEGIKTTCSSKMLENYIPPYNATVVEKLLEEGVIILGKSNLDEFAMGSSTENSAFKTTKNPWDLSRVPGGSSGGSAAA
IAADEAAFALGSDTGGSIRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIGHDPKDSTSL
KIDKPDYTSYLKEDIKGLRIGVAKEFFGEGIEEGVKETVQESIKVLQDLGAEIIDISIPYVEYALPAYYIIASAEASSNL
ARYDGIRYGHIAEKYEDLIDMYMVTRSEGFGKEVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFEKAFEKCDVIIG
PTSPTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIPCGLSDGLPVGLQIIGRHFDEGKILNVAYAFEQANKFSAK
PQAIGGAR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=498, Percent_Identity=39.9598393574297, Blast_Score=361, Evalue=1e-100,
Organism=Homo sapiens, GI195972892, Length=496, Percent_Identity=26.6129032258064, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI166795287, Length=209, Percent_Identity=29.1866028708134, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=440, Percent_Identity=38.6363636363636, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17537465, Length=488, Percent_Identity=25, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17556264, Length=304, Percent_Identity=29.2763157894737, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71990152, Length=329, Percent_Identity=26.7477203647416, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17556276, Length=320, Percent_Identity=29.375, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17556278, Length=320, Percent_Identity=29.375, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=318, Percent_Identity=26.7295597484277, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17538254, Length=330, Percent_Identity=22.4242424242424, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=426, Percent_Identity=40.1408450704225, Blast_Score=270, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6319685, Length=468, Percent_Identity=28.4188034188034, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320448, Length=512, Percent_Identity=23.046875, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24648113, Length=460, Percent_Identity=40.8695652173913, Blast_Score=323, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24652985, Length=492, Percent_Identity=27.4390243902439, Blast_Score=158, Evalue=9e-39,
Organism=Drosophila melanogaster, GI19922090, Length=492, Percent_Identity=27.4390243902439, Blast_Score=158, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24652981, Length=492, Percent_Identity=27.4390243902439, Blast_Score=158, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24652983, Length=492, Percent_Identity=27.4390243902439, Blast_Score=158, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24644968, Length=499, Percent_Identity=27.8557114228457, Blast_Score=141, Evalue=9e-34,
Organism=Drosophila melanogaster, GI161078093, Length=516, Percent_Identity=27.7131782945736, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI45550774, Length=485, Percent_Identity=27.8350515463918, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24648435, Length=485, Percent_Identity=27.8350515463918, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24648437, Length=485, Percent_Identity=27.8350515463918, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI21356731, Length=489, Percent_Identity=25.9713701431493, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24648441, Length=421, Percent_Identity=27.5534441805226, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648439, Length=421, Percent_Identity=27.5534441805226, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53071; Mature: 53071

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELYSLTIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALVTITEDFALQKAKEADE
CCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHEEEEEHHHHHHHHHHHHHH
KIKKGEDTALTGIPVIIKDNISTEGIKTTCSSKMLENYIPPYNATVVEKLLEEGVIILGK
HHHCCCCCEECCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEEC
SNLDEFAMGSSTENSAFKTTKNPWDLSRVPGGSSGGSAAAIAADEAAFALGSDTGGSIRQ
CCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHEEECCCCCCCCCC
PASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIGHDPKDSTSL
CHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEE
KIDKPDYTSYLKEDIKGLRIGVAKEFFGEGIEEGVKETVQESIKVLQDLGAEIIDISIPY
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCH
VEYALPAYYIIASAEASSNLARYDGIRYGHIAEKYEDLIDMYMVTRSEGFGKEVKRRIML
HHHHCCCEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
GTYALSSGYYDAYYKKALKVRTLIKNDFEKAFEKCDVIIGPTSPTVAFKIGERANDPLAM
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEEEECCCCCCCHHH
YLADIYTVSVNIAGLPGISIPCGLSDGLPVGLQIIGRHFDEGKILNVAYAFEQANKFSAK
HHHHHEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEEHHHHHHHCCCC
PQAIGGAR
CCCCCCCC
>Mature Secondary Structure
MELYSLTIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALVTITEDFALQKAKEADE
CCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHEEEEEHHHHHHHHHHHHHH
KIKKGEDTALTGIPVIIKDNISTEGIKTTCSSKMLENYIPPYNATVVEKLLEEGVIILGK
HHHCCCCCEECCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEEC
SNLDEFAMGSSTENSAFKTTKNPWDLSRVPGGSSGGSAAAIAADEAAFALGSDTGGSIRQ
CCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHEEECCCCCCCCCC
PASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIGHDPKDSTSL
CHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEE
KIDKPDYTSYLKEDIKGLRIGVAKEFFGEGIEEGVKETVQESIKVLQDLGAEIIDISIPY
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCH
VEYALPAYYIIASAEASSNLARYDGIRYGHIAEKYEDLIDMYMVTRSEGFGKEVKRRIML
HHHHCCCEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
GTYALSSGYYDAYYKKALKVRTLIKNDFEKAFEKCDVIIGPTSPTVAFKIGERANDPLAM
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEEEECCCCCCCHHH
YLADIYTVSVNIAGLPGISIPCGLSDGLPVGLQIIGRHFDEGKILNVAYAFEQANKFSAK
HHHHHEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEEHHHHHHHCCCC
PQAIGGAR
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA