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The map label for this gene is gatB [H]

Identifier: 167039200

GI number: 167039200

Start: 560503

End: 561930

Strand: Direct

Name: gatB [H]

Synonym: Teth514_0541

Alternate gene names: 167039200

Gene position: 560503-561930 (Clockwise)

Preceding gene: 167039199

Following gene: 167039201

Centisome position: 22.81

GC content: 37.82

Gene sequence:

>1428_bases
ATGAAGTACGAAGCAGTGATTGGCTTAGAGGTCCATGCCGAACTTTTGACAGAGAGCAAAATCTTCTGTGGTTGTACTAC
CAAATTTGGCGGTGAACCTAATACCCACGTATGTCCGGTATGTCTTGGACTTCCCGGAACTTTACCAGTTTTAAATAAAA
AAGTTGTAGAATATGCTGTAAGAGCGGGACTTGCTTTAAACTGCACAATTGCAAACTTCAGCAAGATGGACAGAAAAAAC
TACTTTTATCCTGACTTGCCCAAAGCTTATCAGATTTCTCAATATGACCTTCCCTTGTGCAGCAACGGATATGTAGAAAT
TGAAGTAGATGGTAAGGTGAAGAAAATAGGAATAAAGAGGATACACATTGAAGAAGATGCTGGAAAGTTGCTACATGAAA
ATACAGATGGTTCTTTAGTAGATTACAACCGTGCAGGGGTGCCTCTCATTGAAATAGTTTCTGAACCAGATATGTCTACA
CCTGAAGAAGCTTATCAATACTTAACAAAATTAAAGAGCATTTTAGAGTACACCGAAGTTTCTGACTGCAAAATGCAGGA
AGGTTCTTTAAGAGTTGATACAAATGTGTCTGTGAGGCCTGTGGGAAGTACTGAATTAGGGACAAAAATAGAGCTTAAAA
ATTTAAACTCCTTTAGAGCCGTTCAAAAGGCGTTGGAATATGAAATAAAAAGGCAGATAAAAGTCTTAGAAGAAGGCGGA
ACAATAGTACAAGAGACAAGAAGATGGAATGAGGCAAAAGGAATAACCGAGCCTATGAGGACAAAAGAGGAAGCTCATGA
TTACAGGTATTTCCCTGAACCAGATTTAGTTCCAATAATTGTAACAGATGAGTGGAAAGAAGAGATTAGAAAGTCTTTGC
CTGAAATGCCACACCGCAAGAGAGAGAGATTTATTTCCGAGTACGGTTTACCTGAATATGATGCTAAAATTATAACTTCT
TCAAAGAAAATAGCGGACTTCTTTGAAAAGTGCGCTCTCGAATATGATTCTCCAAAGGCTGTAAGCAATTGGCTTATGGG
AGAATTTTCTCGCCTCATGAATGAGACAGGGAAAGAAATAGACGAAGTACCCGTAACACCGCAAATGTTAGTAAAATTGC
TTAAATTAATTGATAACGGTGTGATAAGCGGATCCATTGCTAAAACTGTTTTTGAAGAGATGTTTGGTACAGGGAAAGAG
CCAGAGGTAATAGTAGAAGAAAAAGGATTGAAACAGATAGCTAATGAGGACGAATTAAGAGAAATTATCAAAAAAGTCAT
AGCTGAAAATCCTAAATCAGTAGAGGATTACAAAAATGGCAAAGAAAAGGCAATGGGCTTTTTGGTAGGGCAAGTTATGA
AGGCGACCAAAGGAAAAGCAAATCCGCAGCTTACTAATCAAATTTTGAAAGAAGAATTGTCGAAATAA

Upstream 100 bases:

>100_bases
GCAGACATTTTGACGAAGGGAAAATATTAAATGTCGCTTATGCCTTTGAACAAGCCAATAAATTTAGTGCAAAACCACAG
GCCATAGGAGGTGCAAGATA

Downstream 100 bases:

>100_bases
ATAGCCTACGCAAATTGTAGGCTACAGATTGATAACAAGCTCCACTTTTTATACATGTAAAAGTGGAGCTTGATTTATAT
TTGTATATAATTGTATATAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKYEAVIGLEVHAELLTESKIFCGCTTKFGGEPNTHVCPVCLGLPGTLPVLNKKVVEYAVRAGLALNCTIANFSKMDRKN
YFYPDLPKAYQISQYDLPLCSNGYVEIEVDGKVKKIGIKRIHIEEDAGKLLHENTDGSLVDYNRAGVPLIEIVSEPDMST
PEEAYQYLTKLKSILEYTEVSDCKMQEGSLRVDTNVSVRPVGSTELGTKIELKNLNSFRAVQKALEYEIKRQIKVLEEGG
TIVQETRRWNEAKGITEPMRTKEEAHDYRYFPEPDLVPIIVTDEWKEEIRKSLPEMPHRKRERFISEYGLPEYDAKIITS
SKKIADFFEKCALEYDSPKAVSNWLMGEFSRLMNETGKEIDEVPVTPQMLVKLLKLIDNGVISGSIAKTVFEEMFGTGKE
PEVIVEEKGLKQIANEDELREIIKKVIAENPKSVEDYKNGKEKAMGFLVGQVMKATKGKANPQLTNQILKEELSK

Sequences:

>Translated_475_residues
MKYEAVIGLEVHAELLTESKIFCGCTTKFGGEPNTHVCPVCLGLPGTLPVLNKKVVEYAVRAGLALNCTIANFSKMDRKN
YFYPDLPKAYQISQYDLPLCSNGYVEIEVDGKVKKIGIKRIHIEEDAGKLLHENTDGSLVDYNRAGVPLIEIVSEPDMST
PEEAYQYLTKLKSILEYTEVSDCKMQEGSLRVDTNVSVRPVGSTELGTKIELKNLNSFRAVQKALEYEIKRQIKVLEEGG
TIVQETRRWNEAKGITEPMRTKEEAHDYRYFPEPDLVPIIVTDEWKEEIRKSLPEMPHRKRERFISEYGLPEYDAKIITS
SKKIADFFEKCALEYDSPKAVSNWLMGEFSRLMNETGKEIDEVPVTPQMLVKLLKLIDNGVISGSIAKTVFEEMFGTGKE
PEVIVEEKGLKQIANEDELREIIKKVIAENPKSVEDYKNGKEKAMGFLVGQVMKATKGKANPQLTNQILKEELSK
>Mature_475_residues
MKYEAVIGLEVHAELLTESKIFCGCTTKFGGEPNTHVCPVCLGLPGTLPVLNKKVVEYAVRAGLALNCTIANFSKMDRKN
YFYPDLPKAYQISQYDLPLCSNGYVEIEVDGKVKKIGIKRIHIEEDAGKLLHENTDGSLVDYNRAGVPLIEIVSEPDMST
PEEAYQYLTKLKSILEYTEVSDCKMQEGSLRVDTNVSVRPVGSTELGTKIELKNLNSFRAVQKALEYEIKRQIKVLEEGG
TIVQETRRWNEAKGITEPMRTKEEAHDYRYFPEPDLVPIIVTDEWKEEIRKSLPEMPHRKRERFISEYGLPEYDAKIITS
SKKIADFFEKCALEYDSPKAVSNWLMGEFSRLMNETGKEIDEVPVTPQMLVKLLKLIDNGVISGSIAKTVFEEMFGTGKE
PEVIVEEKGLKQIANEDELREIIKKVIAENPKSVEDYKNGKEKAMGFLVGQVMKATKGKANPQLTNQILKEELSK

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=496, Percent_Identity=41.5322580645161, Blast_Score=352, Evalue=4e-97,
Organism=Caenorhabditis elegans, GI115534094, Length=476, Percent_Identity=30.0420168067227, Blast_Score=213, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6319391, Length=509, Percent_Identity=29.8624754420432, Blast_Score=222, Evalue=1e-58,
Organism=Drosophila melanogaster, GI45550808, Length=491, Percent_Identity=35.234215885947, Blast_Score=295, Evalue=4e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53701; Mature: 53701

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYEAVIGLEVHAELLTESKIFCGCTTKFGGEPNTHVCPVCLGLPGTLPVLNKKVVEYAV
CCCEEEEEHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHH
RAGLALNCTIANFSKMDRKNYFYPDLPKAYQISQYDLPLCSNGYVEIEVDGKVKKIGIKR
HCCCEEEEEEHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEECCCEEEECEEE
IHIEEDAGKLLHENTDGSLVDYNRAGVPLIEIVSEPDMSTPEEAYQYLTKLKSILEYTEV
EEEECHHHHHHHCCCCCCEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHC
SDCKMQEGSLRVDTNVSVRPVGSTELGTKIELKNLNSFRAVQKALEYEIKRQIKVLEEGG
CCCEECCCCEEECCCCEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
TIVQETRRWNEAKGITEPMRTKEEAHDYRYFPEPDLVPIIVTDEWKEEIRKSLPEMPHRK
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCHHH
RERFISEYGLPEYDAKIITSSKKIADFFEKCALEYDSPKAVSNWLMGEFSRLMNETGKEI
HHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHH
DEVPVTPQMLVKLLKLIDNGVISGSIAKTVFEEMFGTGKEPEVIVEEKGLKQIANEDELR
HCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEECHHHHHHHCCHHHHH
EIIKKVIAENPKSVEDYKNGKEKAMGFLVGQVMKATKGKANPQLTNQILKEELSK
HHHHHHHHCCCCCHHHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYEAVIGLEVHAELLTESKIFCGCTTKFGGEPNTHVCPVCLGLPGTLPVLNKKVVEYAV
CCCEEEEEHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHH
RAGLALNCTIANFSKMDRKNYFYPDLPKAYQISQYDLPLCSNGYVEIEVDGKVKKIGIKR
HCCCEEEEEEHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEECCCEEEECEEE
IHIEEDAGKLLHENTDGSLVDYNRAGVPLIEIVSEPDMSTPEEAYQYLTKLKSILEYTEV
EEEECHHHHHHHCCCCCCEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHC
SDCKMQEGSLRVDTNVSVRPVGSTELGTKIELKNLNSFRAVQKALEYEIKRQIKVLEEGG
CCCEECCCCEEECCCCEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
TIVQETRRWNEAKGITEPMRTKEEAHDYRYFPEPDLVPIIVTDEWKEEIRKSLPEMPHRK
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCHHH
RERFISEYGLPEYDAKIITSSKKIADFFEKCALEYDSPKAVSNWLMGEFSRLMNETGKEI
HHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHH
DEVPVTPQMLVKLLKLIDNGVISGSIAKTVFEEMFGTGKEPEVIVEEKGLKQIANEDELR
HCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEECHHHHHHHCCHHHHH
EIIKKVIAENPKSVEDYKNGKEKAMGFLVGQVMKATKGKANPQLTNQILKEELSK
HHHHHHHHCCCCCHHHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA