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The map label for this gene is ligA [H]

Identifier: 167039197

GI number: 167039197

Start: 556674

End: 558662

Strand: Direct

Name: ligA [H]

Synonym: Teth514_0538

Alternate gene names: 167039197

Gene position: 556674-558662 (Clockwise)

Preceding gene: 167039196

Following gene: 167039198

Centisome position: 22.65

GC content: 35.7

Gene sequence:

>1989_bases
ATGGATCCAAAAGAAAGGATAAAGGAATTGAGAGAAAAAATAAATTATCACAATTACAGATATTACGTTTTGGACCAACC
AGAGATTTCAGACTATGAGTACGACATGCTGATGAGAGAACTTATAGAATTAGAAGAAAAATATCCAGAGCTTAAAACTC
CAGACTCTCCATCCCAAAGAGTAGGTGGGGAGCCTTTAAAAGAATTTGAGCCTTTTACCCATGTAGTTCCAATGTTAAGT
TTGGCTAATGCTTTTTCTGAGGGAGAACTTAGAGACTTTGACAGAAGAGTAAGAGAGGCAGTGGGAGATGTAGAGTACGT
AGTAGAATTAAAAATTGACGGCTTATCTGTGGAACTAATTTATGAAAAAGGCATATTTACTGTGGGTTCTACAAGAGGAG
ACGGGATCGTAGGAGAAAATGTCACACAAAATTTAAAGACTATAAAGTCAATTCCTTTAAGGCTTAAGGATGATGTAAGC
CTTGTTGTAAGAGGAGAGGTCTTCATGCCCCGCGCTTCTTTTGAAAAATTAAATGAGGAGAGAGAAAAATTAGGGGAAAG
CCTTTTTGCAAATCCCAGAAATGCTGCAGCAGGTTCTTTAAGGCAATTAGATCCTAAAGTGACGGCGAAAAGAGATTTAG
ACATTTTTATTTTTAACCTTCAGAAGATTGAGGGTAGAAAATTTAAAACCCACATAGAGACATTAGAATTTTTGAATGAA
CAGGGATTTAAAATAATACCAATTCATAAAAAATGTAGCAATATAGATGAAGTAATAAAAGAAATAGAAGAGATAAGAAA
CTTACGAGATAAGCTGCCTTACGACATTGATGGAGCAGTTGTAAAAGTAAATGACCTTGAAAAGAGAGAAATTTTAGGCC
AGACTGCCAAAGACCCCAGGTGGGCTATTGCTTTTAAATATCCTGCAGAGAGGAAAAAGACAAAAGTCTTGGATATAATA
GTTCAAGTAGGAAGGACTGGAGCCCTTACTCCTACAGCTATATTAGAGCCTGTAGCTATTTCAGGTTCAGTTGTAAGCCG
AGCTACACTTCACAATGAGGACTACATAAAAGAGAAAGACATAAGGATAGGAGACACGGTAATAGTTCAAAAAGCGGGAG
AAATCATTCCTGAAGTAGTAGAAGTGGTAAAAGAAGAGAGGACTGGACAAGAAAGAGAATTTGTAATGCCAGATAGATGC
CCTGAATGCGGTGCTTTAGCTGTCAGGCTTCTGGGTGAGGCTATAAGGCGTTGCACAGGTCTAAATTGTCCTGCACAGCT
TCTAAGAGGCATTATCCACTTTGCTTCAAAAGATGCAATGGATATAGAAGGATTGGGGCCAGCTATAATAAATCAGCTTT
TGTCAAAAGGTCTAATTCACAACATAGCAGATTTGTATTATCTTAAATACGAAGATTTAATACAATTAGAAAGAATGGGG
GACAAATCTGTTAAAAATTTATTAAATGCTATTGAAGAAAGCAAAACAAGAGATTTAGATAGATTGCTCTTTGGCTTGGG
TATAAACTTAATAGGCAGTAAAGCTGCCCAAGTGATTGCTGAGCATTTTAAGACAATGGACAATATCATGAAGGCAAAAT
TTGAAGACTTTACACAGTTGCCTGACATAGGACCTAAGATGGCAAGAAGCATAGTTTCCTTTTTTGCAGAAAAACAAAAT
GTAGAGATAATTGAAAAACTTAAAAATGCTGGAGTAAACATGAAAAAACTTTCAAAAGGAAAAGTAAGCAACATATTTGA
AGGGAAAACTTTTGTTTTGACAGGAGCGTTGGAAAGTTACACGAGAGAAGAAGCTACCAGAATGATTGAAGAAAGAGGTG
GAAAAGTTACAAATTCAGTAAGTAAAAAAACAGACTATGTATTAGTAGGTAAAGATCCTGGTTCTAAACTTAAAAAAGCC
CAAGAATTAGGAATAAAGATAATTGATGAAAAACAATTTGAAGAAATGCTAAAAGGTGAAAATATTTAA

Upstream 100 bases:

>100_bases
GAGAAGAGATTACAGTTGCCTTTCCAAATGTGGGCATAAAAAAGTTAGACTTAAAGTTTGCTCCTATTAAAGCTATTTCT
TAATTTATGGAGGTTGGATT

Downstream 100 bases:

>100_bases
AAACTCTATCCTTCAAAAAATATGTTTTTGTGGTAAACTATAATAGGTGAATAATAAAGGAAAGGTGTGGTAACATGGCT
ATTAGCAGGAGCGAAGTTGA

Product: NAD-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 662; Mature: 662

Protein sequence:

>662_residues
MDPKERIKELREKINYHNYRYYVLDQPEISDYEYDMLMRELIELEEKYPELKTPDSPSQRVGGEPLKEFEPFTHVVPMLS
LANAFSEGELRDFDRRVREAVGDVEYVVELKIDGLSVELIYEKGIFTVGSTRGDGIVGENVTQNLKTIKSIPLRLKDDVS
LVVRGEVFMPRASFEKLNEEREKLGESLFANPRNAAAGSLRQLDPKVTAKRDLDIFIFNLQKIEGRKFKTHIETLEFLNE
QGFKIIPIHKKCSNIDEVIKEIEEIRNLRDKLPYDIDGAVVKVNDLEKREILGQTAKDPRWAIAFKYPAERKKTKVLDII
VQVGRTGALTPTAILEPVAISGSVVSRATLHNEDYIKEKDIRIGDTVIVQKAGEIIPEVVEVVKEERTGQEREFVMPDRC
PECGALAVRLLGEAIRRCTGLNCPAQLLRGIIHFASKDAMDIEGLGPAIINQLLSKGLIHNIADLYYLKYEDLIQLERMG
DKSVKNLLNAIEESKTRDLDRLLFGLGINLIGSKAAQVIAEHFKTMDNIMKAKFEDFTQLPDIGPKMARSIVSFFAEKQN
VEIIEKLKNAGVNMKKLSKGKVSNIFEGKTFVLTGALESYTREEATRMIEERGGKVTNSVSKKTDYVLVGKDPGSKLKKA
QELGIKIIDEKQFEEMLKGENI

Sequences:

>Translated_662_residues
MDPKERIKELREKINYHNYRYYVLDQPEISDYEYDMLMRELIELEEKYPELKTPDSPSQRVGGEPLKEFEPFTHVVPMLS
LANAFSEGELRDFDRRVREAVGDVEYVVELKIDGLSVELIYEKGIFTVGSTRGDGIVGENVTQNLKTIKSIPLRLKDDVS
LVVRGEVFMPRASFEKLNEEREKLGESLFANPRNAAAGSLRQLDPKVTAKRDLDIFIFNLQKIEGRKFKTHIETLEFLNE
QGFKIIPIHKKCSNIDEVIKEIEEIRNLRDKLPYDIDGAVVKVNDLEKREILGQTAKDPRWAIAFKYPAERKKTKVLDII
VQVGRTGALTPTAILEPVAISGSVVSRATLHNEDYIKEKDIRIGDTVIVQKAGEIIPEVVEVVKEERTGQEREFVMPDRC
PECGALAVRLLGEAIRRCTGLNCPAQLLRGIIHFASKDAMDIEGLGPAIINQLLSKGLIHNIADLYYLKYEDLIQLERMG
DKSVKNLLNAIEESKTRDLDRLLFGLGINLIGSKAAQVIAEHFKTMDNIMKAKFEDFTQLPDIGPKMARSIVSFFAEKQN
VEIIEKLKNAGVNMKKLSKGKVSNIFEGKTFVLTGALESYTREEATRMIEERGGKVTNSVSKKTDYVLVGKDPGSKLKKA
QELGIKIIDEKQFEEMLKGENI
>Mature_662_residues
MDPKERIKELREKINYHNYRYYVLDQPEISDYEYDMLMRELIELEEKYPELKTPDSPSQRVGGEPLKEFEPFTHVVPMLS
LANAFSEGELRDFDRRVREAVGDVEYVVELKIDGLSVELIYEKGIFTVGSTRGDGIVGENVTQNLKTIKSIPLRLKDDVS
LVVRGEVFMPRASFEKLNEEREKLGESLFANPRNAAAGSLRQLDPKVTAKRDLDIFIFNLQKIEGRKFKTHIETLEFLNE
QGFKIIPIHKKCSNIDEVIKEIEEIRNLRDKLPYDIDGAVVKVNDLEKREILGQTAKDPRWAIAFKYPAERKKTKVLDII
VQVGRTGALTPTAILEPVAISGSVVSRATLHNEDYIKEKDIRIGDTVIVQKAGEIIPEVVEVVKEERTGQEREFVMPDRC
PECGALAVRLLGEAIRRCTGLNCPAQLLRGIIHFASKDAMDIEGLGPAIINQLLSKGLIHNIADLYYLKYEDLIQLERMG
DKSVKNLLNAIEESKTRDLDRLLFGLGINLIGSKAAQVIAEHFKTMDNIMKAKFEDFTQLPDIGPKMARSIVSFFAEKQN
VEIIEKLKNAGVNMKKLSKGKVSNIFEGKTFVLTGALESYTREEATRMIEERGGKVTNSVSKKTDYVLVGKDPGSKLKKA
QELGIKIIDEKQFEEMLKGENI

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Homo sapiens, GI32528306, Length=81, Percent_Identity=53.0864197530864, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788750, Length=665, Percent_Identity=46.015037593985, Blast_Score=594, Evalue=1e-171,
Organism=Escherichia coli, GI87082305, Length=572, Percent_Identity=23.4265734265734, Blast_Score=116, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; PF00633 HHH [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 74987; Mature: 74987

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPKERIKELREKINYHNYRYYVLDQPEISDYEYDMLMRELIELEEKYPELKTPDSPSQR
CCHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
VGGEPLKEFEPFTHVVPMLSLANAFSEGELRDFDRRVREAVGDVEYVVELKIDGLSVELI
CCCCCHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEEEEEECCEEEEEE
YEKGIFTVGSTRGDGIVGENVTQNLKTIKSIPLRLKDDVSLVVRGEVFMPRASFEKLNEE
EECCEEEECCCCCCCCCCCHHHHHHHHHHHCCCEECCCCEEEEECCEECCCHHHHHHHHH
REKLGESLFANPRNAAAGSLRQLDPKVTAKRDLDIFIFNLQKIEGRKFKTHIETLEFLNE
HHHHHHHHHCCCCCHHHCCHHHCCCCCCCCCCCEEEEEEEEHHCCCHHHHHHHHHHHHHC
QGFKIIPIHKKCSNIDEVIKEIEEIRNLRDKLPYDIDGAVVKVNDLEKREILGQTAKDPR
CCCEEEECHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCCCCCC
WAIAFKYPAERKKTKVLDIIVQVGRTGALTPTAILEPVAISGSVVSRATLHNEDYIKEKD
EEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHCCCHHHHHHHHCCCCCCCCCC
IRIGDTVIVQKAGEIIPEVVEVVKEERTGQEREFVMPDRCPECGALAVRLLGEAIRRCTG
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
LNCPAQLLRGIIHFASKDAMDIEGLGPAIINQLLSKGLIHNIADLYYLKYEDLIQLERMG
CCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DKSVKNLLNAIEESKTRDLDRLLFGLGINLIGSKAAQVIAEHFKTMDNIMKAKFEDFTQL
CHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PDIGPKMARSIVSFFAEKQNVEIIEKLKNAGVNMKKLSKGKVSNIFEGKTFVLTGALESY
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHCCCCHHHHCCCCEEEEECCHHHH
TREEATRMIEERGGKVTNSVSKKTDYVLVGKDPGSKLKKAQELGIKIIDEKQFEEMLKGE
HHHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCCHHHHHHHHCCCEEECHHHHHHHHCCC
NI
CC
>Mature Secondary Structure
MDPKERIKELREKINYHNYRYYVLDQPEISDYEYDMLMRELIELEEKYPELKTPDSPSQR
CCHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
VGGEPLKEFEPFTHVVPMLSLANAFSEGELRDFDRRVREAVGDVEYVVELKIDGLSVELI
CCCCCHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEEEEEECCEEEEEE
YEKGIFTVGSTRGDGIVGENVTQNLKTIKSIPLRLKDDVSLVVRGEVFMPRASFEKLNEE
EECCEEEECCCCCCCCCCCHHHHHHHHHHHCCCEECCCCEEEEECCEECCCHHHHHHHHH
REKLGESLFANPRNAAAGSLRQLDPKVTAKRDLDIFIFNLQKIEGRKFKTHIETLEFLNE
HHHHHHHHHCCCCCHHHCCHHHCCCCCCCCCCCEEEEEEEEHHCCCHHHHHHHHHHHHHC
QGFKIIPIHKKCSNIDEVIKEIEEIRNLRDKLPYDIDGAVVKVNDLEKREILGQTAKDPR
CCCEEEECHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCCCCCC
WAIAFKYPAERKKTKVLDIIVQVGRTGALTPTAILEPVAISGSVVSRATLHNEDYIKEKD
EEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHCCCHHHHHHHHCCCCCCCCCC
IRIGDTVIVQKAGEIIPEVVEVVKEERTGQEREFVMPDRCPECGALAVRLLGEAIRRCTG
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
LNCPAQLLRGIIHFASKDAMDIEGLGPAIINQLLSKGLIHNIADLYYLKYEDLIQLERMG
CCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DKSVKNLLNAIEESKTRDLDRLLFGLGINLIGSKAAQVIAEHFKTMDNIMKAKFEDFTQL
CHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PDIGPKMARSIVSFFAEKQNVEIIEKLKNAGVNMKKLSKGKVSNIFEGKTFVLTGALESY
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHCCCCHHHHCCCCEEEEECCHHHH
TREEATRMIEERGGKVTNSVSKKTDYVLVGKDPGSKLKKAQELGIKIIDEKQFEEMLKGE
HHHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCCHHHHHHHHCCCEEECHHHHHHHHCCC
NI
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA