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The map label for this gene is pcrA [H]

Identifier: 167039196

GI number: 167039196

Start: 554521

End: 556656

Strand: Direct

Name: pcrA [H]

Synonym: Teth514_0537

Alternate gene names: 167039196

Gene position: 554521-556656 (Clockwise)

Preceding gene: 167039195

Following gene: 167039197

Centisome position: 22.57

GC content: 33.94

Gene sequence:

>2136_bases
ATGAACAGCATATTAAATAACCTTAATGACAAACAAAAAGAAGCAGTTATGACTACAGAAGGACCTCTTTTGATATTAGC
GGGAGCTGGAAGCGGAAAAACTCGTGTTTTGACTCATAGAATTGCTTATCTTATAAAAGAGAAAAAAATCTCTCCTTCTA
ACATACTGGCTATCACTTTTACAAATAAAGCGGCAGAAGAAATGAAAACAAGAGTAGAAGATTTATTAGGTTATATAGGT
GACCTGTGGGTATCTACTTTTCATTCTGCCTGTGTGAGAATTTTAAGGCGAGATATTGACAAAATAGGCTATGACAGAAA
TTTCGTCATATTTGATACTACTGACCAAAAGGCTTTAATACAGGAATGCCTTAAAGAGCTAAATTTAAGTGAAAAACAGT
ATCCTGTGAAAACTGTCTTAAATGCTATTTCTTCTGCAAAGGATAAAATGGTAACTCCAGAAGAGTATATTTATGTTTTT
GGAAATGAGTATAGAAGCAAGAAAATAAGTGAAATATACAAGCTATATCAAAAAAAGTTAAAGAAAAACAATGCCCTCGA
TTTTGATGACATAATTATAAAAACTATTGAACTTTTTAAGGAAAGCCCCGAAGTTTTAGACTTTTACCAGAGGAAATTTA
AATACATAATGGTGGACGAATATCAAGACACAAATACGCCTCAATACCATTTTGTGAACATGTTAGCCCAGAAATACAGA
AACTTATGTGTAGTTGGTGATGATGACCAAAGCATATATGGTTGGAGAGGTGCAGATGTAAAAAACATATTAAACTTTGA
GAAGGATTATCCTGACGCTAAAGTTATAAAATTAGAGCAAAACTATCGTTCTACCAAAACAATATTGGAAGCAGCCAATT
ATGTCATAGATAATAATATAAGAAGGAAGAAAAAGACTTTATGGACAAATAATGAACAAGGAGAAAAGATAATTCTCTGC
GAATTAGACAATGAGAGGGAAGAAGCGGAATTTGTAATACAGGAAATAATCAGCTTAAAAGAAAGAGAAAATAGAAGCTT
TAGGGATTTTGCTATTTTATACAGGACAAATGCCCAGTCCCGTGCTTTTGAGGAAGCCTTAATGAGGGTGCGAATTCCTT
ACAAAGTGGTCGGAGCTTTAAGGTTTTACGACAGAAAAGAAATAAAAGACATTATTGCCTATTTGAGAATACTTGTAAAT
CCTTATGATGACATATCTTTTAAAAGGATAATAAACGTTCCTAAAAGAGGTATTGGAGCTGCAACCATTGAGGCATTAGA
GGCTACAGCTTTGGAGAAAGACACCAGTCTTTTTTTTGCAATAGATGATGCAAAAGTAAGCCAAAGGGCTAAAAATAGCC
TTTTAGAATTTAAGGAATTTATTTTAGAATTAATTGATAAAAAAGATACAATGACTGTCAGTGAAGTAATAAATTACATT
TTAGAAGAGACTGGATATGTAGAGGAGTTAGAAAAAGAAGAATCAGAACAAGCAGAAGGAAGGATAGAAAACCTAAAAGA
GTTTTTAAATGCGGCTTATGAATTTGAAGAGTCTTCAGAGGACAAATCTTTAGAAGCATTTTTGGCCGGGATAACATTGG
TATCTGATATTGACTTGGCTGGAGAGATTGGAGAAAGCGTTGTTCTCATGACGTTGCATTCGGCGAAGGGATTAGAGTTT
CCTGTTGTCTTTATGGTAGGCATGGAAGAAGGAATATTTCCTTCTTTTAAGTCATTTACAGATGAATATGAATTAGAAGA
GGAAAGACGGCTCTGTTATGTTGGCATTACGAGGTCTAAAGAAAAACTCTATATGACCTATGCGAGGAGGAGAAATTTAT
ACGGCAAATCTCAGTACAGCTCCTATTCCCGTTTTATAAGCGAAATACCTGAGAGGTTTTTAGTAAGATACCATGAGTTA
TCAAAGCCAAGGGAAGAGTATAGGTCTGTATCTAGCTATGTAGAAAGGAAAACTTATGAAAAGGCTCAATATAATTTAGG
AGATAAAGTAGAGCATAAAATATGGGGGATAGGTACTGTAGTAAAAGTAGAAGGAGAAGAGATTACAGTTGCCTTTCCAA
ATGTGGGCATAAAAAAGTTAGACTTAAAGTTTGCTCCTATTAAAGCTATTTCTTAA

Upstream 100 bases:

>100_bases
AATTATGGAGCAAATGGATATAAAATTATATTAGACAGGTTAGAATCAGAATCCCGGGAATAAAACCGGGCTTTTTTAAA
ACTAAATTGGAGTGAGAAAA

Downstream 100 bases:

>100_bases
TTTATGGAGGTTGGATTATGGATCCAAAAGAAAGGATAAAGGAATTGAGAGAAAAAATAAATTATCACAATTACAGATAT
TACGTTTTGGACCAACCAGA

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 711; Mature: 711

Protein sequence:

>711_residues
MNSILNNLNDKQKEAVMTTEGPLLILAGAGSGKTRVLTHRIAYLIKEKKISPSNILAITFTNKAAEEMKTRVEDLLGYIG
DLWVSTFHSACVRILRRDIDKIGYDRNFVIFDTTDQKALIQECLKELNLSEKQYPVKTVLNAISSAKDKMVTPEEYIYVF
GNEYRSKKISEIYKLYQKKLKKNNALDFDDIIIKTIELFKESPEVLDFYQRKFKYIMVDEYQDTNTPQYHFVNMLAQKYR
NLCVVGDDDQSIYGWRGADVKNILNFEKDYPDAKVIKLEQNYRSTKTILEAANYVIDNNIRRKKKTLWTNNEQGEKIILC
ELDNEREEAEFVIQEIISLKERENRSFRDFAILYRTNAQSRAFEEALMRVRIPYKVVGALRFYDRKEIKDIIAYLRILVN
PYDDISFKRIINVPKRGIGAATIEALEATALEKDTSLFFAIDDAKVSQRAKNSLLEFKEFILELIDKKDTMTVSEVINYI
LEETGYVEELEKEESEQAEGRIENLKEFLNAAYEFEESSEDKSLEAFLAGITLVSDIDLAGEIGESVVLMTLHSAKGLEF
PVVFMVGMEEGIFPSFKSFTDEYELEEERRLCYVGITRSKEKLYMTYARRRNLYGKSQYSSYSRFISEIPERFLVRYHEL
SKPREEYRSVSSYVERKTYEKAQYNLGDKVEHKIWGIGTVVKVEGEEITVAFPNVGIKKLDLKFAPIKAIS

Sequences:

>Translated_711_residues
MNSILNNLNDKQKEAVMTTEGPLLILAGAGSGKTRVLTHRIAYLIKEKKISPSNILAITFTNKAAEEMKTRVEDLLGYIG
DLWVSTFHSACVRILRRDIDKIGYDRNFVIFDTTDQKALIQECLKELNLSEKQYPVKTVLNAISSAKDKMVTPEEYIYVF
GNEYRSKKISEIYKLYQKKLKKNNALDFDDIIIKTIELFKESPEVLDFYQRKFKYIMVDEYQDTNTPQYHFVNMLAQKYR
NLCVVGDDDQSIYGWRGADVKNILNFEKDYPDAKVIKLEQNYRSTKTILEAANYVIDNNIRRKKKTLWTNNEQGEKIILC
ELDNEREEAEFVIQEIISLKERENRSFRDFAILYRTNAQSRAFEEALMRVRIPYKVVGALRFYDRKEIKDIIAYLRILVN
PYDDISFKRIINVPKRGIGAATIEALEATALEKDTSLFFAIDDAKVSQRAKNSLLEFKEFILELIDKKDTMTVSEVINYI
LEETGYVEELEKEESEQAEGRIENLKEFLNAAYEFEESSEDKSLEAFLAGITLVSDIDLAGEIGESVVLMTLHSAKGLEF
PVVFMVGMEEGIFPSFKSFTDEYELEEERRLCYVGITRSKEKLYMTYARRRNLYGKSQYSSYSRFISEIPERFLVRYHEL
SKPREEYRSVSSYVERKTYEKAQYNLGDKVEHKIWGIGTVVKVEGEEITVAFPNVGIKKLDLKFAPIKAIS
>Mature_711_residues
MNSILNNLNDKQKEAVMTTEGPLLILAGAGSGKTRVLTHRIAYLIKEKKISPSNILAITFTNKAAEEMKTRVEDLLGYIG
DLWVSTFHSACVRILRRDIDKIGYDRNFVIFDTTDQKALIQECLKELNLSEKQYPVKTVLNAISSAKDKMVTPEEYIYVF
GNEYRSKKISEIYKLYQKKLKKNNALDFDDIIIKTIELFKESPEVLDFYQRKFKYIMVDEYQDTNTPQYHFVNMLAQKYR
NLCVVGDDDQSIYGWRGADVKNILNFEKDYPDAKVIKLEQNYRSTKTILEAANYVIDNNIRRKKKTLWTNNEQGEKIILC
ELDNEREEAEFVIQEIISLKERENRSFRDFAILYRTNAQSRAFEEALMRVRIPYKVVGALRFYDRKEIKDIIAYLRILVN
PYDDISFKRIINVPKRGIGAATIEALEATALEKDTSLFFAIDDAKVSQRAKNSLLEFKEFILELIDKKDTMTVSEVINYI
LEETGYVEELEKEESEQAEGRIENLKEFLNAAYEFEESSEDKSLEAFLAGITLVSDIDLAGEIGESVVLMTLHSAKGLEF
PVVFMVGMEEGIFPSFKSFTDEYELEEERRLCYVGITRSKEKLYMTYARRRNLYGKSQYSSYSRFISEIPERFLVRYHEL
SKPREEYRSVSSYVERKTYEKAQYNLGDKVEHKIWGIGTVVKVEGEEITVAFPNVGIKKLDLKFAPIKAIS

Specific function: DNA helicase used for plasmid rolling-circle replication and also involved in UV repair [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=722, Percent_Identity=41.9667590027701, Blast_Score=565, Evalue=1e-162,
Organism=Escherichia coli, GI48994965, Length=676, Percent_Identity=40.9763313609467, Blast_Score=447, Evalue=1e-126,
Organism=Escherichia coli, GI1787196, Length=361, Percent_Identity=27.4238227146814, Blast_Score=116, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6322369, Length=731, Percent_Identity=28.4541723666211, Blast_Score=239, Evalue=8e-64,
Organism=Saccharomyces cerevisiae, GI6324477, Length=689, Percent_Identity=24.3831640058055, Blast_Score=121, Evalue=3e-28,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 82564; Mature: 82564

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSILNNLNDKQKEAVMTTEGPLLILAGAGSGKTRVLTHRIAYLIKEKKISPSNILAITF
CCHHHHCCCHHHHHHEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEE
TNKAAEEMKTRVEDLLGYIGDLWVSTFHSACVRILRRDIDKIGYDRNFVIFDTTDQKALI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHH
QECLKELNLSEKQYPVKTVLNAISSAKDKMVTPEEYIYVFGNEYRSKKISEIYKLYQKKL
HHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
KKNNALDFDDIIIKTIELFKESPEVLDFYQRKFKYIMVDEYQDTNTPQYHFVNMLAQKYR
HCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHHHHHHC
NLCVVGDDDQSIYGWRGADVKNILNFEKDYPDAKVIKLEQNYRSTKTILEAANYVIDNNI
CEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCH
RRKKKTLWTNNEQGEKIILCELDNEREEAEFVIQEIISLKERENRSFRDFAILYRTNAQS
HHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCHH
RAFEEALMRVRIPYKVVGALRFYDRKEIKDIIAYLRILVNPYDDISFKRIINVPKRGIGA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCH
ATIEALEATALEKDTSLFFAIDDAKVSQRAKNSLLEFKEFILELIDKKDTMTVSEVINYI
HHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHH
LEETGYVEELEKEESEQAEGRIENLKEFLNAAYEFEESSEDKSLEAFLAGITLVSDIDLA
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHH
GEIGESVVLMTLHSAKGLEFPVVFMVGMEEGIFPSFKSFTDEYELEEERRLCYVGITRSK
HHCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHCHHHCCCCCCCEEEEEEECCC
EKLYMTYARRRNLYGKSQYSSYSRFISEIPERFLVRYHELSKPREEYRSVSSYVERKTYE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
KAQYNLGDKVEHKIWGIGTVVKVEGEEITVAFPNVGIKKLDLKFAPIKAIS
HHHCCCCCHHHHHHCCCEEEEEEECCEEEEECCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MNSILNNLNDKQKEAVMTTEGPLLILAGAGSGKTRVLTHRIAYLIKEKKISPSNILAITF
CCHHHHCCCHHHHHHEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEE
TNKAAEEMKTRVEDLLGYIGDLWVSTFHSACVRILRRDIDKIGYDRNFVIFDTTDQKALI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHH
QECLKELNLSEKQYPVKTVLNAISSAKDKMVTPEEYIYVFGNEYRSKKISEIYKLYQKKL
HHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
KKNNALDFDDIIIKTIELFKESPEVLDFYQRKFKYIMVDEYQDTNTPQYHFVNMLAQKYR
HCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHHHHHHC
NLCVVGDDDQSIYGWRGADVKNILNFEKDYPDAKVIKLEQNYRSTKTILEAANYVIDNNI
CEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCH
RRKKKTLWTNNEQGEKIILCELDNEREEAEFVIQEIISLKERENRSFRDFAILYRTNAQS
HHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCHH
RAFEEALMRVRIPYKVVGALRFYDRKEIKDIIAYLRILVNPYDDISFKRIINVPKRGIGA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCH
ATIEALEATALEKDTSLFFAIDDAKVSQRAKNSLLEFKEFILELIDKKDTMTVSEVINYI
HHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHH
LEETGYVEELEKEESEQAEGRIENLKEFLNAAYEFEESSEDKSLEAFLAGITLVSDIDLA
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHH
GEIGESVVLMTLHSAKGLEFPVVFMVGMEEGIFPSFKSFTDEYELEEERRLCYVGITRSK
HHCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHCHHHCCCCCCCEEEEEEECCC
EKLYMTYARRRNLYGKSQYSSYSRFISEIPERFLVRYHELSKPREEYRSVSSYVERKTYE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
KAQYNLGDKVEHKIWGIGTVVKVEGEEITVAFPNVGIKKLDLKFAPIKAIS
HHHCCCCCHHHHHHCCCEEEEEEECCEEEEECCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377 [H]