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The map label for this gene is purN [H]

Identifier: 167039183

GI number: 167039183

Start: 540081

End: 540695

Strand: Direct

Name: purN [H]

Synonym: Teth514_0524

Alternate gene names: 167039183

Gene position: 540081-540695 (Clockwise)

Preceding gene: 167039182

Following gene: 167039184

Centisome position: 21.98

GC content: 36.42

Gene sequence:

>615_bases
ATGAACCTTGTGGTGATGGCATCAGGTAACGGGACTGATTTGCAGTCAATAATTGATGCCATTGAAGAAGGATATATAAA
TGCCAGGATAATTGCCGTCATTAGCGATAAAAAAGGAGCTTATGCTCTTGAAAGGGCAAAAAAACACGGAATTGCTACTT
ACTGCCTTCCCAAAAAAGAATTGAAAGAAAATTTCCAAAGAGAGTTATTAAAACTTCTTGAAAAATTGAATCCGGATGGA
ATAATTCTTGCAGGTTTTTTGACAATTCTCAGTGGAGAAATTGTAGAAAGATTTGAAAACAAGATAATAAATATACATCC
CTCACTTATCCCTGCCTTTTGTGGCAAAGGATTTTATGGAATGAAGGTACATCAGGCGGTCTATGAATACGGAGTAAAAT
ACACCGGCTGTACTGTTCACTTTGTGGACAGTGGTGCTGATACCGGACCTATTATTCTTCAAGAAGTTGTGAAAATTGAT
GAAGAGGATACGCCAGAGGCCATTGCAAAAAAAGTACTGGAAGTAGAACACAAAGTTTTACCTTATGCAGTTAAGCTTTT
TACAGAGGGGAAGTTAAAAGTCGAAGGTAGAAAAGTGAAAATATTAAAGTTTTAA

Upstream 100 bases:

>100_bases
ACCCTTCTGATGTAGATAAGGCTTTAGAAAAATTAAATGGCATAGGTGAAAAAGCTTATGTAATAGGGGAGATAGTAGAA
AGCGAAGGCGGTGTAATTTT

Downstream 100 bases:

>100_bases
AAGGGATTATAAAACTGTAAAAAGGAGGCAATTCTATGGCCAAAAAAGCGTTAATAAGTGTTTCAAAAAAAGAAGGCATA
GTTGAATTTGCCAAAAAGCT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MNLVVMASGNGTDLQSIIDAIEEGYINARIIAVISDKKGAYALERAKKHGIATYCLPKKELKENFQRELLKLLEKLNPDG
IILAGFLTILSGEIVERFENKIINIHPSLIPAFCGKGFYGMKVHQAVYEYGVKYTGCTVHFVDSGADTGPIILQEVVKID
EEDTPEAIAKKVLEVEHKVLPYAVKLFTEGKLKVEGRKVKILKF

Sequences:

>Translated_204_residues
MNLVVMASGNGTDLQSIIDAIEEGYINARIIAVISDKKGAYALERAKKHGIATYCLPKKELKENFQRELLKLLEKLNPDG
IILAGFLTILSGEIVERFENKIINIHPSLIPAFCGKGFYGMKVHQAVYEYGVKYTGCTVHFVDSGADTGPIILQEVVKID
EEDTPEAIAKKVLEVEHKVLPYAVKLFTEGKLKVEGRKVKILKF
>Mature_204_residues
MNLVVMASGNGTDLQSIIDAIEEGYINARIIAVISDKKGAYALERAKKHGIATYCLPKKELKENFQRELLKLLEKLNPDG
IILAGFLTILSGEIVERFENKIINIHPSLIPAFCGKGFYGMKVHQAVYEYGVKYTGCTVHFVDSGADTGPIILQEVVKID
EEDTPEAIAKKVLEVEHKVLPYAVKLFTEGKLKVEGRKVKILKF

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=194, Percent_Identity=38.659793814433, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI209869995, Length=194, Percent_Identity=38.659793814433, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI209869993, Length=194, Percent_Identity=38.659793814433, Blast_Score=139, Evalue=2e-33,
Organism=Escherichia coli, GI1788846, Length=197, Percent_Identity=38.5786802030457, Blast_Score=147, Evalue=5e-37,
Organism=Escherichia coli, GI1787483, Length=141, Percent_Identity=37.5886524822695, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17567511, Length=184, Percent_Identity=34.7826086956522, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320616, Length=196, Percent_Identity=33.1632653061224, Blast_Score=86, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24582400, Length=192, Percent_Identity=36.4583333333333, Blast_Score=131, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22676; Mature: 22676

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLVVMASGNGTDLQSIIDAIEEGYINARIIAVISDKKGAYALERAKKHGIATYCLPKKE
CCEEEEECCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHCCCEEEECCHHH
LKENFQRELLKLLEKLNPDGIILAGFLTILSGEIVERFENKIINIHPSLIPAFCGKGFYG
HHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHCCCCCC
MKVHQAVYEYGVKYTGCTVHFVDSGADTGPIILQEVVKIDEEDTPEAIAKKVLEVEHKVL
HHHHHHHHHCCCEECCEEEEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
PYAVKLFTEGKLKVEGRKVKILKF
HHHHEEEECCEEEECCCEEEEEEC
>Mature Secondary Structure
MNLVVMASGNGTDLQSIIDAIEEGYINARIIAVISDKKGAYALERAKKHGIATYCLPKKE
CCEEEEECCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHCCCEEEECCHHH
LKENFQRELLKLLEKLNPDGIILAGFLTILSGEIVERFENKIINIHPSLIPAFCGKGFYG
HHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHCCCCCC
MKVHQAVYEYGVKYTGCTVHFVDSGADTGPIILQEVVKIDEEDTPEAIAKKVLEVEHKVL
HHHHHHHHHCCCEECCEEEEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
PYAVKLFTEGKLKVEGRKVKILKF
HHHHEEEECCEEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]