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The map label for this gene is aprX [H]

Identifier: 167039168

GI number: 167039168

Start: 522558

End: 523796

Strand: Reverse

Name: aprX [H]

Synonym: Teth514_0509

Alternate gene names: 167039168

Gene position: 523796-522558 (Counterclockwise)

Preceding gene: 167039222

Following gene: 167039154

Centisome position: 21.32

GC content: 36.32

Gene sequence:

>1239_bases
ATGAATCTTATTCCAATGTTTGCTTTTTCTAAAATAGCAAAGGAAATTTATGTATCAAATAAAATCGATAAAAGGCTTTT
AAAAAAAGCATTATATTTAAGAAGTGAATGTGTTCCTGTAATTTTGTATTCCCATTTATCTTATGACATTTTAAAAGAAA
AAATCGAAAAAATGGGAGGAAGTATAAAATTTGAACTACCAATCATAAAAGCCTGGTCAGTAAATTTACCTTGTGATAAA
TTGAAAAATTTCGCTACATTAAAAGGAATTCATTTTATAGCAGAAGACTCCGCTGTTAAGCTGCAGCTTTATATTGCCAC
TCAAGAAATCGCCTCTCGAAATGCGAACGATTTAGGCTACACCGGTAAGGGAGTAACAATTGCCTTTTTAGACACTGGAA
TATATCCTCACCCTGATTTTACCAAGCCTAAAAACCGCATAGTGGCATTTCACGATGTAGTAAATGGAAAAAAACAACCT
TACGATGACAATGGCCACGGCACTCATGTAGCTGGTGACGCTGCTGGAAATGGATATGCTTCTAATGGCAAATACAAAGG
CGTAGCCCCAGAAGCTAATATAGTAGCAGTAAAAGTATTAGATGCCTATGGCAGAGGTTTATCCTCTGACATATTAACAG
GAATGCAATGGATTTTAGACAACAAAGACAAATACAATATACGGGTAGTTTCTCTGTCAATAGGTGAAACTCCTTCTCTT
CCTGCTTTTTTAGACCCTTTGGTGAGAGGAGTAGATACTCTTTGGAGAAATGGATTAGTAGTAACTGTTGCAGCCGGAAA
CTCAGGTCCTAATTACAACACAATAACTTCCCCTGGCACCAGTAAAAATGCCATAACTGTAGGAGCAGTAGATGACAAAA
GAACCTCCGACATAAGTGATGACGAAATAGCTCAATTTTCTGGAAGAGGTTCGCCTTATCTATACAAACCAGACATAGTA
GCACCAGGTGTAAAAATAGTCTCTACTGCTTCAGAAAATATACCTTTTGGTGCAGATGAAATTACGATAAATAAAGCATA
TAGAACAGCAACAGGGACTTCTATGGCGACTCCAATGGCAGCAGGAGCAGCAGCTCTACTCCTTGAAAAAAATCCTAATT
TGACTAATGTACAAATAAAAAATATTTTAAAATCGACAGCAATAAAAATTGACGATGCAGGTCTATGGACACAAGGCAGT
GGAATGATAAATATAGAAGAGGCATTAAAAAAAGTATGA

Upstream 100 bases:

>100_bases
ATAATATGATATAACTTCATACGGTGACACTTTTTAAATTTCCACTAACAAATTTTTATATTTCACATATTGTGTTAATA
GCATGAAAGGAGGTACGCTA

Downstream 100 bases:

>100_bases
GGGGGTTAACCCCTCGTTTCAGTTTGTTCACAAAGCTAAAAATATTCAAAGGAGATATTTTGCATCGTCACTCCGATGTT
CCAAAGCGACAAAACTAAAC

Product: peptidase S8/S53 subtilisin kexin sedolisin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MNLIPMFAFSKIAKEIYVSNKIDKRLLKKALYLRSECVPVILYSHLSYDILKEKIEKMGGSIKFELPIIKAWSVNLPCDK
LKNFATLKGIHFIAEDSAVKLQLYIATQEIASRNANDLGYTGKGVTIAFLDTGIYPHPDFTKPKNRIVAFHDVVNGKKQP
YDDNGHGTHVAGDAAGNGYASNGKYKGVAPEANIVAVKVLDAYGRGLSSDILTGMQWILDNKDKYNIRVVSLSIGETPSL
PAFLDPLVRGVDTLWRNGLVVTVAAGNSGPNYNTITSPGTSKNAITVGAVDDKRTSDISDDEIAQFSGRGSPYLYKPDIV
APGVKIVSTASENIPFGADEITINKAYRTATGTSMATPMAAGAAALLLEKNPNLTNVQIKNILKSTAIKIDDAGLWTQGS
GMINIEEALKKV

Sequences:

>Translated_412_residues
MNLIPMFAFSKIAKEIYVSNKIDKRLLKKALYLRSECVPVILYSHLSYDILKEKIEKMGGSIKFELPIIKAWSVNLPCDK
LKNFATLKGIHFIAEDSAVKLQLYIATQEIASRNANDLGYTGKGVTIAFLDTGIYPHPDFTKPKNRIVAFHDVVNGKKQP
YDDNGHGTHVAGDAAGNGYASNGKYKGVAPEANIVAVKVLDAYGRGLSSDILTGMQWILDNKDKYNIRVVSLSIGETPSL
PAFLDPLVRGVDTLWRNGLVVTVAAGNSGPNYNTITSPGTSKNAITVGAVDDKRTSDISDDEIAQFSGRGSPYLYKPDIV
APGVKIVSTASENIPFGADEITINKAYRTATGTSMATPMAAGAAALLLEKNPNLTNVQIKNILKSTAIKIDDAGLWTQGS
GMINIEEALKKV
>Mature_412_residues
MNLIPMFAFSKIAKEIYVSNKIDKRLLKKALYLRSECVPVILYSHLSYDILKEKIEKMGGSIKFELPIIKAWSVNLPCDK
LKNFATLKGIHFIAEDSAVKLQLYIATQEIASRNANDLGYTGKGVTIAFLDTGIYPHPDFTKPKNRIVAFHDVVNGKKQP
YDDNGHGTHVAGDAAGNGYASNGKYKGVAPEANIVAVKVLDAYGRGLSSDILTGMQWILDNKDKYNIRVVSLSIGETPSL
PAFLDPLVRGVDTLWRNGLVVTVAAGNSGPNYNTITSPGTSKNAITVGAVDDKRTSDISDDEIAQFSGRGSPYLYKPDIV
APGVKIVSTASENIPFGADEITINKAYRTATGTSMATPMAAGAAALLLEKNPNLTNVQIKNILKSTAIKIDDAGLWTQGS
GMINIEEALKKV

Specific function: Displays serine protease activity. Seems to have a broad substrate specificity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Cytoplasm (Probable). Note=Is leaked to the culture medium during the late stationary phase owing to cell lysis [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI4506775, Length=337, Percent_Identity=29.3768545994065, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI186972143, Length=265, Percent_Identity=27.5471698113208, Blast_Score=78, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17533391, Length=260, Percent_Identity=28.0769230769231, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71983555, Length=301, Percent_Identity=30.2325581395349, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25141268, Length=301, Percent_Identity=30.2325581395349, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319893, Length=253, Percent_Identity=35.1778656126482, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6320775, Length=265, Percent_Identity=35.0943396226415, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324576, Length=308, Percent_Identity=30.8441558441558, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI45550681, Length=300, Percent_Identity=32.3333333333333, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24642488, Length=334, Percent_Identity=27.5449101796407, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24642484, Length=334, Percent_Identity=27.5449101796407, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24642486, Length=334, Percent_Identity=27.5449101796407, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI17137360, Length=260, Percent_Identity=27.6923076923077, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24653270, Length=260, Percent_Identity=27.6923076923077, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500 [H]

Pfam domain/function: PF00082 Peptidase_S8 [H]

EC number: NA

Molecular weight: Translated: 44480; Mature: 44480

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLIPMFAFSKIAKEIYVSNKIDKRLLKKALYLRSECVPVILYSHLSYDILKEKIEKMGG
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEHHHHCCHHHHHHHHHHHCC
SIKFELPIIKAWSVNLPCDKLKNFATLKGIHFIAEDSAVKLQLYIATQEIASRNANDLGY
CEEEEEEEEEEEECCCCHHHHHCHHHHCCEEEEECCCEEEEEEEEEEHHHHCCCCCCCCC
TGKGVTIAFLDTGIYPHPDFTKPKNRIVAFHDVVNGKKQPYDDNGHGTHVAGDAAGNGYA
CCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEHHCCCCCCCCCCCCCCEEECCCCCCCCC
SNGKYKGVAPEANIVAVKVLDAYGRGLSSDILTGMQWILDNKDKYNIRVVSLSIGETPSL
CCCEECCCCCCCCEEEEEEEHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCC
PAFLDPLVRGVDTLWRNGLVVTVAAGNSGPNYNTITSPGTSKNAITVGAVDDKRTSDISD
CHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCH
DEIAQFSGRGSPYLYKPDIVAPGVKIVSTASENIPFGADEITINKAYRTATGTSMATPMA
HHHHHHCCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCEEEEECCEEECCCCCCCCCHH
AGAAALLLEKNPNLTNVQIKNILKSTAIKIDDAGLWTQGSGMINIEEALKKV
CCCEEEEEECCCCCCCHHHHHHHHHHCEEEECCCCEECCCCEEEHHHHHHCC
>Mature Secondary Structure
MNLIPMFAFSKIAKEIYVSNKIDKRLLKKALYLRSECVPVILYSHLSYDILKEKIEKMGG
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEHHHHCCHHHHHHHHHHHCC
SIKFELPIIKAWSVNLPCDKLKNFATLKGIHFIAEDSAVKLQLYIATQEIASRNANDLGY
CEEEEEEEEEEEECCCCHHHHHCHHHHCCEEEEECCCEEEEEEEEEEHHHHCCCCCCCCC
TGKGVTIAFLDTGIYPHPDFTKPKNRIVAFHDVVNGKKQPYDDNGHGTHVAGDAAGNGYA
CCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEHHCCCCCCCCCCCCCCEEECCCCCCCCC
SNGKYKGVAPEANIVAVKVLDAYGRGLSSDILTGMQWILDNKDKYNIRVVSLSIGETPSL
CCCEECCCCCCCCEEEEEEEHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCC
PAFLDPLVRGVDTLWRNGLVVTVAAGNSGPNYNTITSPGTSKNAITVGAVDDKRTSDISD
CHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCH
DEIAQFSGRGSPYLYKPDIVAPGVKIVSTASENIPFGADEITINKAYRTATGTSMATPMA
HHHHHHCCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCEEEEECCEEECCCCCCCCCHH
AGAAALLLEKNPNLTNVQIKNILKSTAIKIDDAGLWTQGSGMINIEEALKKV
CCCEEEEEECCCCCCCHHHHHHHHHHCEEEECCCCEECCCCEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]