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The map label for this gene is bioB [H]

Identifier: 167039222

GI number: 167039222

Start: 586169

End: 587206

Strand: Reverse

Name: bioB [H]

Synonym: Teth514_0563

Alternate gene names: 167039222

Gene position: 587206-586169 (Counterclockwise)

Preceding gene: 167039230

Following gene: 167039168

Centisome position: 23.9

GC content: 32.66

Gene sequence:

>1038_bases
TTGTTAGAACTAATAAAAAAAGCTGAAAAAACACACTCTCTCACAAAGAAAGAAATAGTTGAGCTTTTAAAGGATGATCA
ATATAACGATGAATTATTTAAAGCCGCGGATAGAGTAAGAAAAAAATATGTGGGAGATGAAGTTCATTTAAGAGGGCTTA
TTGAATTTTCTAATATATGTAAGCAAAATTGCTTATATTGTGGTCTAAGAAGAGATAACAAAAATATAAAACGTTATAGA
TTAAAGCCAGAACAGATAATTAATTTTGCCAAAAATGCCCGCAATTTAGGATATAGAACTGTAGTATTGCAGTCTGGCGA
AGATGATTTTTTCAATGTTGAAAGGATGACTAAAATCATAAAATCCATAAAACAACTCGATATTGCAATAACTCTAAGTA
TAGGCGAAAAAACAAGAGAAGAATATAAGGCATATAAAGAAGCTGGAGCAGATAGATATTTGTTAAGAATAGAAACAACG
GATAAAGAACTTTATGAAAAACTCGATCCTAATATGAGTCATGAAAATAGAAAAAGGTGTCTTAAAGATTTAAAAGAATT
AGGTTACGAAGTTGGAACTGGATGTCTCATAGGACTTCCTGGACAAACTATAGAATCCATTGCAGATGACATTCTATTTT
TTAAAGAAATTGATGCTGATATGATAGGGGTTGGTCCATTTATCCCGAATCCCGATACACCATTAAAAAATGAAAAGGGA
GGCACATTTGAACTCAGTTTAAAAGTTATGGCCATAACAAGGCTTTTGATGCCTGATATTAATATTCCGGCAACTACAGC
AATGGAAACCCTAAATGTTAATGGAAGGTTGATAGCACTTCGTAGTGGAGCAAATGTTGTAATGCCAAATGTTACAGAAG
GGGAATACAGAAAATTGTACGCATTATATCCAGGCAAAATCTGTATAAATGACACTCCCGCCCATTGCTTTAGCTGTATT
ACTGGTAAAATAAATAGCATAGGCAGACCAATTGCAAAAGATTATGGCTATAGGAAAAATGTCATGTCAAAAAAGTAA

Upstream 100 bases:

>100_bases
AAAATTACTCATTTTTATTTAGCCACAAAAAACTTTGCTATCTTTTTAATAATTTTATTTGCCAATTTGAAGTTTAAATT
TATTGAGGTAGGTGAACTTT

Downstream 100 bases:

>100_bases
GGGACGGGGTCTTCCCCGTCTTTTATGCATACATATTTCCATAGGGTCTTTTGCCTATCGGATGCAATTTGACAAAAGAA
TGATTCATAATCTTTTGAAA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MLELIKKAEKTHSLTKKEIVELLKDDQYNDELFKAADRVRKKYVGDEVHLRGLIEFSNICKQNCLYCGLRRDNKNIKRYR
LKPEQIINFAKNARNLGYRTVVLQSGEDDFFNVERMTKIIKSIKQLDIAITLSIGEKTREEYKAYKEAGADRYLLRIETT
DKELYEKLDPNMSHENRKRCLKDLKELGYEVGTGCLIGLPGQTIESIADDILFFKEIDADMIGVGPFIPNPDTPLKNEKG
GTFELSLKVMAITRLLMPDINIPATTAMETLNVNGRLIALRSGANVVMPNVTEGEYRKLYALYPGKICINDTPAHCFSCI
TGKINSIGRPIAKDYGYRKNVMSKK

Sequences:

>Translated_345_residues
MLELIKKAEKTHSLTKKEIVELLKDDQYNDELFKAADRVRKKYVGDEVHLRGLIEFSNICKQNCLYCGLRRDNKNIKRYR
LKPEQIINFAKNARNLGYRTVVLQSGEDDFFNVERMTKIIKSIKQLDIAITLSIGEKTREEYKAYKEAGADRYLLRIETT
DKELYEKLDPNMSHENRKRCLKDLKELGYEVGTGCLIGLPGQTIESIADDILFFKEIDADMIGVGPFIPNPDTPLKNEKG
GTFELSLKVMAITRLLMPDINIPATTAMETLNVNGRLIALRSGANVVMPNVTEGEYRKLYALYPGKICINDTPAHCFSCI
TGKINSIGRPIAKDYGYRKNVMSKK
>Mature_345_residues
MLELIKKAEKTHSLTKKEIVELLKDDQYNDELFKAADRVRKKYVGDEVHLRGLIEFSNICKQNCLYCGLRRDNKNIKRYR
LKPEQIINFAKNARNLGYRTVVLQSGEDDFFNVERMTKIIKSIKQLDIAITLSIGEKTREEYKAYKEAGADRYLLRIETT
DKELYEKLDPNMSHENRKRCLKDLKELGYEVGTGCLIGLPGQTIESIADDILFFKEIDADMIGVGPFIPNPDTPLKNEKG
GTFELSLKVMAITRLLMPDINIPATTAMETLNVNGRLIALRSGANVVMPNVTEGEYRKLYALYPGKICINDTPAHCFSCI
TGKINSIGRPIAKDYGYRKNVMSKK

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6321725, Length=298, Percent_Identity=22.8187919463087, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 39301; Mature: 39301

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLELIKKAEKTHSLTKKEIVELLKDDQYNDELFKAADRVRKKYVGDEVHLRGLIEFSNIC
CHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
KQNCLYCGLRRDNKNIKRYRLKPEQIINFAKNARNLGYRTVVLQSGEDDFFNVERMTKII
HHHHEEEEEECCCCCHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHH
KSIKQLDIAITLSIGEKTREEYKAYKEAGADRYLLRIETTDKELYEKLDPNMSHENRKRC
HHHHHCCEEEEEECCHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHCCCCCHHHHHHH
LKDLKELGYEVGTGCLIGLPGQTIESIADDILFFKEIDADMIGVGPFIPNPDTPLKNEKG
HHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCC
GTFELSLKVMAITRLLMPDINIPATTAMETLNVNGRLIALRSGANVVMPNVTEGEYRKLY
CEEEEEEHHHHHHHHHCCCCCCCCHHHHHEECCCCEEEEEECCCCEEECCCCCCCCEEEE
ALYPGKICINDTPAHCFSCITGKINSIGRPIAKDYGYRKNVMSKK
EECCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHCCCC
>Mature Secondary Structure
MLELIKKAEKTHSLTKKEIVELLKDDQYNDELFKAADRVRKKYVGDEVHLRGLIEFSNIC
CHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
KQNCLYCGLRRDNKNIKRYRLKPEQIINFAKNARNLGYRTVVLQSGEDDFFNVERMTKII
HHHHEEEEEECCCCCHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHH
KSIKQLDIAITLSIGEKTREEYKAYKEAGADRYLLRIETTDKELYEKLDPNMSHENRKRC
HHHHHCCEEEEEECCHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHCCCCCHHHHHHH
LKDLKELGYEVGTGCLIGLPGQTIESIADDILFFKEIDADMIGVGPFIPNPDTPLKNEKG
HHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCC
GTFELSLKVMAITRLLMPDINIPATTAMETLNVNGRLIALRSGANVVMPNVTEGEYRKLY
CEEEEEEHHHHHHHHHCCCCCCCCHHHHHEECCCCEEEEEECCCCEEECCCCCCCCEEEE
ALYPGKICINDTPAHCFSCITGKINSIGRPIAKDYGYRKNVMSKK
EECCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA