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The map label for this gene is prfB [H]

Identifier: 167039139

GI number: 167039139

Start: 490780

End: 491772

Strand: Direct

Name: prfB [H]

Synonym: Teth514_0478

Alternate gene names: 167039139

Gene position: 490780-491772 (Clockwise)

Preceding gene: 167039138

Following gene: 167039140

Centisome position: 19.97

GC content: 38.47

Gene sequence:

>993_bases
ATGTCTGATCCTGCTTTTTGGAGCGATATAAAAAAGTCACAGGAATTAGCTAAAAAACAAAAAGCCTTAAAAGAACTTTT
AGAAGAGTACAATTCTCTTGTGTCAAAATGGGAAGACTTGAGTACTCTTATTGAATTGGGATTAGAAGAAGGGGATGAAT
CAATAACTGACGAAGTAGACAAAGAGTACAAAGAGTTAAAGAAAAAGTTAGAAGACTTAAAGATAAAAACACTATTAAAT
GGCCTATATGATAAAAACAATGCCATACTTTCTATTCATGCAGGCTCAGGTGGTACAGAGGCTCAAGATTGGGCTGAAAT
GCTACTTCGCATGTATACAAGATGGGCTAATGACAAAGGTTTTGAAGTAGAAACCCTTGACTATTTGCAAGGAGAAGAAG
CAGGAATAAAAAGTGTCACTCTAATGATAAAAGGGCCTTTTGCCTATGGGTATCTTAAATCAGAGGCAGGTGTTCACCGC
CTTGTGAGGATATCTCCTTTTGATGCCGCCGGTAGAAGGCATACTTCATTTGCCCTTGTGGAAGTACTTCCTGAATTAGA
AAATGATATTGAAGTGGAAATAAGGCCTGAGGATTTAAAAATTGACACTTATAGGGCTTCGGGTGCGGGAGGACAGTATG
TAAATAAGACGGAGTCTGCTGTAAGGATTACTCACCTTCCAACAGGCATAGTGGTTTCCTGTCAAAGTGAAAGGTCTCAG
ATTCAAAACAGAGAAACTGCCATGAAAATGCTTAAAGCTAAACTTTTGGACCTCATGATGAAAGAGAAGAAAGAAAAAAT
CGAAGACTTAAAAGGAGAGCATAGAGAGGCAGCATGGGGCAACCAAATAAGGTCTTATGTCTTCCAGCCTTATACCCTTG
TTAAAGACCACAGGACAAATTATGAAGTGGGGAATGTTCAGGCAGTGATGGATGGGGAAATAGATGGATTTATAAACGCT
TATCTAAAGCAAAAATCACAAGGGATATCTTAA

Upstream 100 bases:

>100_bases
AAAGCAGAAGTTTCAAACATGATTGAAGAAATAAAAGAAATGGGGGTTTCTCTTTGACATAGAAAGTGTCAAAAGAGAAA
TAGAAGAAATAGACAAAAAG

Downstream 100 bases:

>100_bases
AAAGGTATCCCTTTAAATTTAATCTATGTATTTCCTAAGAGAAAGGAGTAAGTCATGAATAGGATAAAAGATATATTAAA
GAAAGAATTTGGATTAAAAG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSDPAFWSDIKKSQELAKKQKALKELLEEYNSLVSKWEDLSTLIELGLEEGDESITDEVDKEYKELKKKLEDLKIKTLLN
GLYDKNNAILSIHAGSGGTEAQDWAEMLLRMYTRWANDKGFEVETLDYLQGEEAGIKSVTLMIKGPFAYGYLKSEAGVHR
LVRISPFDAAGRRHTSFALVEVLPELENDIEVEIRPEDLKIDTYRASGAGGQYVNKTESAVRITHLPTGIVVSCQSERSQ
IQNRETAMKMLKAKLLDLMMKEKKEKIEDLKGEHREAAWGNQIRSYVFQPYTLVKDHRTNYEVGNVQAVMDGEIDGFINA
YLKQKSQGIS

Sequences:

>Translated_330_residues
MSDPAFWSDIKKSQELAKKQKALKELLEEYNSLVSKWEDLSTLIELGLEEGDESITDEVDKEYKELKKKLEDLKIKTLLN
GLYDKNNAILSIHAGSGGTEAQDWAEMLLRMYTRWANDKGFEVETLDYLQGEEAGIKSVTLMIKGPFAYGYLKSEAGVHR
LVRISPFDAAGRRHTSFALVEVLPELENDIEVEIRPEDLKIDTYRASGAGGQYVNKTESAVRITHLPTGIVVSCQSERSQ
IQNRETAMKMLKAKLLDLMMKEKKEKIEDLKGEHREAAWGNQIRSYVFQPYTLVKDHRTNYEVGNVQAVMDGEIDGFINA
YLKQKSQGIS
>Mature_329_residues
SDPAFWSDIKKSQELAKKQKALKELLEEYNSLVSKWEDLSTLIELGLEEGDESITDEVDKEYKELKKKLEDLKIKTLLNG
LYDKNNAILSIHAGSGGTEAQDWAEMLLRMYTRWANDKGFEVETLDYLQGEEAGIKSVTLMIKGPFAYGYLKSEAGVHRL
VRISPFDAAGRRHTSFALVEVLPELENDIEVEIRPEDLKIDTYRASGAGGQYVNKTESAVRITHLPTGIVVSCQSERSQI
QNRETAMKMLKAKLLDLMMKEKKEKIEDLKGEHREAAWGNQIRSYVFQPYTLVKDHRTNYEVGNVQAVMDGEIDGFINAY
LKQKSQGIS

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=231, Percent_Identity=42.4242424242424, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI34577120, Length=305, Percent_Identity=36.7213114754098, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI166795305, Length=145, Percent_Identity=46.2068965517241, Blast_Score=129, Evalue=5e-30,
Organism=Escherichia coli, GI2367172, Length=323, Percent_Identity=49.5356037151703, Blast_Score=332, Evalue=2e-92,
Organism=Escherichia coli, GI1787462, Length=246, Percent_Identity=41.0569105691057, Blast_Score=186, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17542784, Length=253, Percent_Identity=36.3636363636364, Blast_Score=118, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6321295, Length=333, Percent_Identity=33.9339339339339, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI19921226, Length=230, Percent_Identity=40.8695652173913, Blast_Score=166, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 37461; Mature: 37330

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPAFWSDIKKSQELAKKQKALKELLEEYNSLVSKWEDLSTLIELGLEEGDESITDEVD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KEYKELKKKLEDLKIKTLLNGLYDKNNAILSIHAGSGGTEAQDWAEMLLRMYTRWANDKG
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCC
FEVETLDYLQGEEAGIKSVTLMIKGPFAYGYLKSEAGVHRLVRISPFDAAGRRHTSFALV
CEEEHHHHHCCCCCCCCEEEEEEECCHHHHHHHCCCCHHHEEEECCCCCCCCCCHHHHHH
EVLPELENDIEVEIRPEDLKIDTYRASGAGGQYVNKTESAVRITHLPTGIVVSCQSERSQ
HHHHHCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECHHHHH
IQNRETAMKMLKAKLLDLMMKEKKEKIEDLKGEHREAAWGNQIRSYVFQPYTLVKDHRTN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHEECCCCCC
YEVGNVQAVMDGEIDGFINAYLKQKSQGIS
CCCCCEEEEECCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SDPAFWSDIKKSQELAKKQKALKELLEEYNSLVSKWEDLSTLIELGLEEGDESITDEVD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KEYKELKKKLEDLKIKTLLNGLYDKNNAILSIHAGSGGTEAQDWAEMLLRMYTRWANDKG
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCC
FEVETLDYLQGEEAGIKSVTLMIKGPFAYGYLKSEAGVHRLVRISPFDAAGRRHTSFALV
CEEEHHHHHCCCCCCCCEEEEEEECCHHHHHHHCCCCHHHEEEECCCCCCCCCCHHHHHH
EVLPELENDIEVEIRPEDLKIDTYRASGAGGQYVNKTESAVRITHLPTGIVVSCQSERSQ
HHHHHCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECHHHHH
IQNRETAMKMLKAKLLDLMMKEKKEKIEDLKGEHREAAWGNQIRSYVFQPYTLVKDHRTN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHEECCCCCC
YEVGNVQAVMDGEIDGFINAYLKQKSQGIS
CCCCCEEEEECCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9696765; 9384377; 1901557; 1408743 [H]