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The map label for this gene is metK [H]

Identifier: 167039106

GI number: 167039106

Start: 459426

End: 460613

Strand: Direct

Name: metK [H]

Synonym: Teth514_0445

Alternate gene names: 167039106

Gene position: 459426-460613 (Clockwise)

Preceding gene: 167039105

Following gene: 167039107

Centisome position: 18.7

GC content: 38.38

Gene sequence:

>1188_bases
ATGCGTAGATTGTTTACATCTGAATCAGTCACTGAGGGGCATCCTGATAAAATTTGTGATCAGATTTCTGATGCGATATT
AGATGAAATTTTGAAAAAAGATCCTTATGCCAGAGTTGCTTGTGAGACTGCTGTAACTACCGGTTTGGTTTTAGTAATGG
GCGAAATTACTACAGATTGTTATGTAGACATACCGAAAGTTGTAAGGAAAGTAGTAGAAGAAATTGGCTATACTAGAGCA
AAATTCGGTTTTGACGCTGATACTTGTGCGGTTATCACTTCTATTGATGAGCAATCACCGGATATTGCCCTCGGTGTGGA
CAAGGCATTAGAAGCTAAAAGAGGAGAACTTTCAGATTCGGAAATTGAGGCTATTGGCGCTGGAGACCAGGGTTTAATGT
TTGGTTTTGCTTGCGATGAAACAGAAGAATTAATGCCTATGCCTATAATGCTAGCTCACAAGCTTGCAAGAAGACTGGCG
GAAGTTAGAAAGAACGGGACATTAAGCTATTTAAGACCAGATGGTAAAACACAAGTAACAGTAGAGTATGAAGATGACAG
GCCTGTGAGGGTAGATTCTGTTGTAGTATCAGCTCAACATGCACCTGAGATTGATCACGATACTATTGAAAGAGATATTA
TTGAACATGTAATAAAACCTATAATTCCTGAAAATATGATAGATGATAAGACTAAAATTTTTGTAAATCCCACAGGAAGG
TTTGTAATTGGTGGACCTCAAGGTGATAGCGGACTTACAGGAAGAAAAATTATTGTAGACACCTATGGCGGATATGCAAG
GCACGGGGGCGGAGCTTTTTCTGGTAAAGACCCTACAAAAGTTGATAGATCTGCAAGTTATGCTGCAAGATATGTGGCAA
AAAATATTGTGGCAGCAGGACTTGCTAAGAAGTGTGAAGTGCAATTAGCTTATGCGATAGGTGTTGCTACACCTCTTGAA
ATAGGTATTGATACTTTTGGCACAGGTAAAATATCAGATGAAAAGATTTCGGAAATTGTAAAACAAGTTTTTGATTTAAG
ACCTGCAGCTATAATTAGGGATTTAGATTTAAGAAGACCTATTTATAGACAAGTTGCAGCTTATGGCCACTTTGGAAGAC
ACGACTTAGACCTCCCATGGGAGAAAACAGACAAAGTAGATATATTAAGAAAATTGGCAGGAATGTAA

Upstream 100 bases:

>100_bases
AAGGTGCTAATTCTCTCAGAAGCATGCTTCTGAAAGATGAGGGTATGTGCCTCTTCTTTTGGAAGGGGTTTTATTTTGCT
TTTAAGGAGGAATAGAAAAA

Downstream 100 bases:

>100_bases
ATTTGAAAGCCCGGGTAAACCCGGGCTTCTGGCATTTTTTATATATCTTTTTTAAATGCCATAGAATTTATGTTTTGTAA
ACGCTTCGCTACCTTAATTA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MRRLFTSESVTEGHPDKICDQISDAILDEILKKDPYARVACETAVTTGLVLVMGEITTDCYVDIPKVVRKVVEEIGYTRA
KFGFDADTCAVITSIDEQSPDIALGVDKALEAKRGELSDSEIEAIGAGDQGLMFGFACDETEELMPMPIMLAHKLARRLA
EVRKNGTLSYLRPDGKTQVTVEYEDDRPVRVDSVVVSAQHAPEIDHDTIERDIIEHVIKPIIPENMIDDKTKIFVNPTGR
FVIGGPQGDSGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSASYAARYVAKNIVAAGLAKKCEVQLAYAIGVATPLE
IGIDTFGTGKISDEKISEIVKQVFDLRPAAIIRDLDLRRPIYRQVAAYGHFGRHDLDLPWEKTDKVDILRKLAGM

Sequences:

>Translated_395_residues
MRRLFTSESVTEGHPDKICDQISDAILDEILKKDPYARVACETAVTTGLVLVMGEITTDCYVDIPKVVRKVVEEIGYTRA
KFGFDADTCAVITSIDEQSPDIALGVDKALEAKRGELSDSEIEAIGAGDQGLMFGFACDETEELMPMPIMLAHKLARRLA
EVRKNGTLSYLRPDGKTQVTVEYEDDRPVRVDSVVVSAQHAPEIDHDTIERDIIEHVIKPIIPENMIDDKTKIFVNPTGR
FVIGGPQGDSGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSASYAARYVAKNIVAAGLAKKCEVQLAYAIGVATPLE
IGIDTFGTGKISDEKISEIVKQVFDLRPAAIIRDLDLRRPIYRQVAAYGHFGRHDLDLPWEKTDKVDILRKLAGM
>Mature_395_residues
MRRLFTSESVTEGHPDKICDQISDAILDEILKKDPYARVACETAVTTGLVLVMGEITTDCYVDIPKVVRKVVEEIGYTRA
KFGFDADTCAVITSIDEQSPDIALGVDKALEAKRGELSDSEIEAIGAGDQGLMFGFACDETEELMPMPIMLAHKLARRLA
EVRKNGTLSYLRPDGKTQVTVEYEDDRPVRVDSVVVSAQHAPEIDHDTIERDIIEHVIKPIIPENMIDDKTKIFVNPTGR
FVIGGPQGDSGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSASYAARYVAKNIVAAGLAKKCEVQLAYAIGVATPLE
IGIDTFGTGKISDEKISEIVKQVFDLRPAAIIRDLDLRRPIYRQVAAYGHFGRHDLDLPWEKTDKVDILRKLAGM

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=387, Percent_Identity=57.8811369509044, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI4557737, Length=382, Percent_Identity=55.4973821989529, Blast_Score=414, Evalue=1e-116,
Organism=Escherichia coli, GI1789311, Length=394, Percent_Identity=61.1675126903553, Blast_Score=505, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17538498, Length=391, Percent_Identity=54.7314578005115, Blast_Score=430, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17538494, Length=401, Percent_Identity=53.3665835411471, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25145633, Length=395, Percent_Identity=53.4177215189873, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17551082, Length=391, Percent_Identity=52.6854219948849, Blast_Score=417, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI32565905, Length=401, Percent_Identity=49.1271820448878, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI32565911, Length=344, Percent_Identity=52.6162790697674, Blast_Score=358, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI25145635, Length=348, Percent_Identity=51.1494252873563, Blast_Score=350, Evalue=5e-97,
Organism=Caenorhabditis elegans, GI32565903, Length=327, Percent_Identity=51.9877675840979, Blast_Score=332, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI32565909, Length=76, Percent_Identity=59.2105263157895, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI32565907, Length=38, Percent_Identity=76.3157894736842, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=383, Percent_Identity=56.3968668407311, Blast_Score=432, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6320710, Length=389, Percent_Identity=54.7557840616967, Blast_Score=430, Evalue=1e-121,
Organism=Drosophila melanogaster, GI45552159, Length=389, Percent_Identity=54.4987146529563, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580529, Length=389, Percent_Identity=54.4987146529563, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580537, Length=389, Percent_Identity=54.4987146529563, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580533, Length=389, Percent_Identity=54.4987146529563, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580535, Length=389, Percent_Identity=54.4987146529563, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580531, Length=389, Percent_Identity=54.4987146529563, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580527, Length=389, Percent_Identity=53.4704370179949, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580525, Length=389, Percent_Identity=53.4704370179949, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580541, Length=377, Percent_Identity=52.2546419098143, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580539, Length=127, Percent_Identity=54.3307086614173, Blast_Score=132, Evalue=6e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43364; Mature: 43364

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLFTSESVTEGHPDKICDQISDAILDEILKKDPYARVACETAVTTGLVLVMGEITTDC
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEHHCCHH
YVDIPKVVRKVVEEIGYTRAKFGFDADTCAVITSIDEQSPDIALGVDKALEAKRGELSDS
EECHHHHHHHHHHHCCCCHHHCCCCCCHHHEEEECCCCCCCEEECCHHHHHHHCCCCCCC
EIEAIGAGDQGLMFGFACDETEELMPMPIMLAHKLARRLAEVRKNGTLSYLRPDGKTQVT
CEEEECCCCCCEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEE
VEYEDDRPVRVDSVVVSAQHAPEIDHDTIERDIIEHVIKPIIPENMIDDKTKIFVNPTGR
EEECCCCCEEEHHEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCC
FVIGGPQGDSGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSASYAARYVAKNIVAAG
EEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LAKKCEVQLAYAIGVATPLEIGIDTFGTGKISDEKISEIVKQVFDLRPAAIIRDLDLRRP
HHHHHHEEEEEEECCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCH
IYRQVAAYGHFGRHDLDLPWEKTDKVDILRKLAGM
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MRRLFTSESVTEGHPDKICDQISDAILDEILKKDPYARVACETAVTTGLVLVMGEITTDC
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEHHCCHH
YVDIPKVVRKVVEEIGYTRAKFGFDADTCAVITSIDEQSPDIALGVDKALEAKRGELSDS
EECHHHHHHHHHHHCCCCHHHCCCCCCHHHEEEECCCCCCCEEECCHHHHHHHCCCCCCC
EIEAIGAGDQGLMFGFACDETEELMPMPIMLAHKLARRLAEVRKNGTLSYLRPDGKTQVT
CEEEECCCCCCEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEE
VEYEDDRPVRVDSVVVSAQHAPEIDHDTIERDIIEHVIKPIIPENMIDDKTKIFVNPTGR
EEECCCCCEEEHHEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCC
FVIGGPQGDSGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSASYAARYVAKNIVAAG
EEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LAKKCEVQLAYAIGVATPLEIGIDTFGTGKISDEKISEIVKQVFDLRPAAIIRDLDLRRP
HHHHHHEEEEEEECCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCH
IYRQVAAYGHFGRHDLDLPWEKTDKVDILRKLAGM
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA