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The map label for this gene is gcvH [H]

Identifier: 167038900

GI number: 167038900

Start: 243025

End: 243405

Strand: Reverse

Name: gcvH [H]

Synonym: Teth514_0231

Alternate gene names: 167038900

Gene position: 243405-243025 (Counterclockwise)

Preceding gene: 167038901

Following gene: 167038899

Centisome position: 9.91

GC content: 39.63

Gene sequence:

>381_bases
ATGGAAGTTTTAGAAGGCTTGTATTACTCAAAAGACCACGAATGGGTCAAAGTAGAAGGAGATAAAGCATACATAGGAAT
AACTGACTATGCACAGCATTCCTTAGGAAACATAGTGTACATTGAGCTTCCAGAAGTAGGTGCAGAACTCTCGGCTGGCG
ATGTACTTGGAGTCGTAGAATCTGTAAAAGCTGCATCTGATGTTTACACACCTGTAGATGGAAAAGTACTTGAAGTAAAC
AATGCTATCGTTGACGACCCCTCTTTAGTAAACAATGACCCCTATGGCAGTTGGATGGCATTAGTAGAACTCAAAGACAA
GTCACAGCTTGATAATTTGATGACAGCCGAAGAATATAAAAAATTTTTAGATGAGGAGTGA

Upstream 100 bases:

>100_bases
AAATCAAATTGAAATTGTGATAAGAAATAAGCCACTTAAGGCTTTAATCATAAGCAAAAACTTTTACAAGAAAAATTATA
AAAAATAGGAGGTTTTTGGT

Downstream 100 bases:

>100_bases
GAGCATGTTTCCTTACCTTCCTGCCTCCTCCAATGACGAAAAAGAAATGCTTAAAACAATAGGAAAAAATTCTATAGAAG
AGCTATTTGAAAACATACCT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 126

Protein sequence:

>126_residues
MEVLEGLYYSKDHEWVKVEGDKAYIGITDYAQHSLGNIVYIELPEVGAELSAGDVLGVVESVKAASDVYTPVDGKVLEVN
NAIVDDPSLVNNDPYGSWMALVELKDKSQLDNLMTAEEYKKFLDEE

Sequences:

>Translated_126_residues
MEVLEGLYYSKDHEWVKVEGDKAYIGITDYAQHSLGNIVYIELPEVGAELSAGDVLGVVESVKAASDVYTPVDGKVLEVN
NAIVDDPSLVNNDPYGSWMALVELKDKSQLDNLMTAEEYKKFLDEE
>Mature_126_residues
MEVLEGLYYSKDHEWVKVEGDKAYIGITDYAQHSLGNIVYIELPEVGAELSAGDVLGVVESVKAASDVYTPVDGKVLEVN
NAIVDDPSLVNNDPYGSWMALVELKDKSQLDNLMTAEEYKKFLDEE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=116, Percent_Identity=43.1034482758621, Blast_Score=111, Evalue=1e-25,
Organism=Homo sapiens, GI89057342, Length=116, Percent_Identity=42.2413793103448, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1789271, Length=122, Percent_Identity=50, Blast_Score=127, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17551294, Length=122, Percent_Identity=47.5409836065574, Blast_Score=125, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17507493, Length=122, Percent_Identity=43.4426229508197, Blast_Score=112, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6319272, Length=124, Percent_Identity=45.1612903225806, Blast_Score=114, Evalue=5e-27,
Organism=Drosophila melanogaster, GI17865652, Length=118, Percent_Identity=49.1525423728814, Blast_Score=116, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13959; Mature: 13959

Theoretical pI: Translated: 3.89; Mature: 3.89

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVLEGLYYSKDHEWVKVEGDKAYIGITDYAQHSLGNIVYIELPEVGAELSAGDVLGVVE
CCCCCCCEECCCCCEEEEECCEEEEEEHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHH
SVKAASDVYTPVDGKVLEVNNAIVDDPSLVNNDPYGSWMALVELKDKSQLDNLMTAEEYK
HHHHHHHCCCCCCCEEEEECCCEECCCCCCCCCCCCCEEEEEEECCHHHHHHHCCHHHHH
KFLDEE
HHHCCC
>Mature Secondary Structure
MEVLEGLYYSKDHEWVKVEGDKAYIGITDYAQHSLGNIVYIELPEVGAELSAGDVLGVVE
CCCCCCCEECCCCCEEEEECCEEEEEEHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHH
SVKAASDVYTPVDGKVLEVNNAIVDDPSLVNNDPYGSWMALVELKDKSQLDNLMTAEEYK
HHHHHHHCCCCCCCEEEEECCCEECCCCCCCCCCCCCEEEEEEECCHHHHHHHCCHHHHH
KFLDEE
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA