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The map label for this gene is ywkE [H]

Identifier: 167038766

GI number: 167038766

Start: 91423

End: 92262

Strand: Direct

Name: ywkE [H]

Synonym: Teth514_0093

Alternate gene names: 167038766

Gene position: 91423-92262 (Clockwise)

Preceding gene: 167038765

Following gene: 167038767

Centisome position: 3.72

GC content: 33.21

Gene sequence:

>840_bases
TTGAAAGTATATGAAGTGATAAATTTAGGGGCAAAAGAACTTCAAGGCATTTGCGAAAATCCTAAACTTGAAGCGGAACT
GCTCCTTGCCTACTGTTTGGGGATGGATAGGATAAATCTAATAATTAAAAGAGAAGATGAAGTGGAGGAAGGGGAGTTAG
CAAAGTTTTTAGGACTTTTAAATATGAGAAAATCTCACATTCCTTATCAGTACATTGTAAAAAAACATTATTTTATGGGT
TTGGAGTTTTTCGTGGATGAAAATGTATTGATTCCAAGGCCTGAGACGGAAATACTTGTGGAAGAAGTATTAAAAAGGCT
TAAGAAAGGTAATACACTAATTGACATAGGGACAGGCAGTGGTGCTATAGCTGTAAGTGTAGTCAAATATTTTCCTGATT
GCTTTGTATATGCTGTCGATATTAGTAGAAAAGCTCTTGAGGTGGCAAAATATAATGCAAAAAAACACAACGTTTTAGAT
AAAATTGTTTTTATAGAAAGTGATGTTTTTTCACAGGTTCCTAAGAATATAAAGTTTGATTTTATTGTATCAAATCCACC
CTATATTAAAAGAGGCGAACTTGAAACCCTTCAAGAAGAAGTAAAAAAAGAACCTATTATAGCGTTGGATGGAGGAGAAG
ATGGGCTTTTCTTTTATAAAAAGATTATAGAGGGAGCTCCTTTTTATCTAAATCCTGAAGGGGCAATTTGCTTTGAAATG
GGATATGACCAAAAAGAGGAGGTAACTGATTTATTGACAAAAGGTGGTTTTAAAAATATTGAAGTGATAAAGGATTTATC
GGGAATTGATAGAGTTGTAATAGCAAGGTATAAGTTATAG

Upstream 100 bases:

>100_bases
CTGTTACAAAAACTTACTACAAGAGAGCCTGATGACAATCAATTAGAAGTTGCTATTGCATCACTAAAAAGTGTTTTGGA
GGACGAGGGAGGACAAGAAT

Downstream 100 bases:

>100_bases
TTTTCACAAATAGCAATATATGATATAATCAATCTTTGAGGTGAGATAATGATTGATAAATTGCAAGCAATAGAAGATAG
ATATGTAGAATTAAGTCAAA

Product: HemK family modification methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKVYEVINLGAKELQGICENPKLEAELLLAYCLGMDRINLIIKREDEVEEGELAKFLGLLNMRKSHIPYQYIVKKHYFMG
LEFFVDENVLIPRPETEILVEEVLKRLKKGNTLIDIGTGSGAIAVSVVKYFPDCFVYAVDISRKALEVAKYNAKKHNVLD
KIVFIESDVFSQVPKNIKFDFIVSNPPYIKRGELETLQEEVKKEPIIALDGGEDGLFFYKKIIEGAPFYLNPEGAICFEM
GYDQKEEVTDLLTKGGFKNIEVIKDLSGIDRVVIARYKL

Sequences:

>Translated_279_residues
MKVYEVINLGAKELQGICENPKLEAELLLAYCLGMDRINLIIKREDEVEEGELAKFLGLLNMRKSHIPYQYIVKKHYFMG
LEFFVDENVLIPRPETEILVEEVLKRLKKGNTLIDIGTGSGAIAVSVVKYFPDCFVYAVDISRKALEVAKYNAKKHNVLD
KIVFIESDVFSQVPKNIKFDFIVSNPPYIKRGELETLQEEVKKEPIIALDGGEDGLFFYKKIIEGAPFYLNPEGAICFEM
GYDQKEEVTDLLTKGGFKNIEVIKDLSGIDRVVIARYKL
>Mature_279_residues
MKVYEVINLGAKELQGICENPKLEAELLLAYCLGMDRINLIIKREDEVEEGELAKFLGLLNMRKSHIPYQYIVKKHYFMG
LEFFVDENVLIPRPETEILVEEVLKRLKKGNTLIDIGTGSGAIAVSVVKYFPDCFVYAVDISRKALEVAKYNAKKHNVLD
KIVFIESDVFSQVPKNIKFDFIVSNPPYIKRGELETLQEEVKKEPIIALDGGEDGLFFYKKIIEGAPFYLNPEGAICFEM
GYDQKEEVTDLLTKGGFKNIEVIKDLSGIDRVVIARYKL

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=209, Percent_Identity=35.4066985645933, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1787463, Length=260, Percent_Identity=33.4615384615385, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI87082085, Length=177, Percent_Identity=38.9830508474576, Blast_Score=130, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6324266, Length=201, Percent_Identity=28.8557213930348, Blast_Score=72, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24582226, Length=215, Percent_Identity=32.093023255814, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 31784; Mature: 31784

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVYEVINLGAKELQGICENPKLEAELLLAYCLGMDRINLIIKREDEVEEGELAKFLGLL
CCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHH
NMRKSHIPYQYIVKKHYFMGLEFFVDENVLIPRPETEILVEEVLKRLKKGNTLIDIGTGS
HHHHHCCCHHHHHHHHHHHCEEEEECCCEECCCCCHHHHHHHHHHHHHCCCEEEEECCCC
GAIAVSVVKYFPDCFVYAVDISRKALEVAKYNAKKHNVLDKIVFIESDVFSQVPKNIKFD
CHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCHHHHHHHHHHEEECHHHHHCCCCCEEE
FIVSNPPYIKRGELETLQEEVKKEPIIALDGGEDGLFFYKKIIEGAPFYLNPEGAICFEM
EEECCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCCEEECCCCCEEEEE
GYDQKEEVTDLLTKGGFKNIEVIKDLSGIDRVVIARYKL
CCCCHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHEEECC
>Mature Secondary Structure
MKVYEVINLGAKELQGICENPKLEAELLLAYCLGMDRINLIIKREDEVEEGELAKFLGLL
CCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHH
NMRKSHIPYQYIVKKHYFMGLEFFVDENVLIPRPETEILVEEVLKRLKKGNTLIDIGTGS
HHHHHCCCHHHHHHHHHHHCEEEEECCCEECCCCCHHHHHHHHHHHHHCCCEEEEECCCC
GAIAVSVVKYFPDCFVYAVDISRKALEVAKYNAKKHNVLDKIVFIESDVFSQVPKNIKFD
CHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCHHHHHHHHHHEEECHHHHHCCCCCEEE
FIVSNPPYIKRGELETLQEEVKKEPIIALDGGEDGLFFYKKIIEGAPFYLNPEGAICFEM
EEECCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCCEEECCCCCEEEEE
GYDQKEEVTDLLTKGGFKNIEVIKDLSGIDRVVIARYKL
CCCCHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]