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The map label for this gene is prfA [H]

Identifier: 167038767

GI number: 167038767

Start: 92311

End: 93381

Strand: Direct

Name: prfA [H]

Synonym: Teth514_0094

Alternate gene names: 167038767

Gene position: 92311-93381 (Clockwise)

Preceding gene: 167038766

Following gene: 167038768

Centisome position: 3.76

GC content: 35.85

Gene sequence:

>1071_bases
ATGATTGATAAATTGCAAGCAATAGAAGATAGATATGTAGAATTAAGTCAAAAAATAAGTGACCCTAATATTATTTCAAA
TGTAAATGAATGGAGAAAGTATGTAAAAGAGCATGCGGCTATTGAAGATATTGTGTTAAAATACAGAGAATACAAAAAAG
TTTTGGAAGATATAGAGGCTACAAAAGAGTTGTTGTCTTCAAATGATGAAGAACTTAAAGAGATGGCAGAGGAAGAACTT
TCTCAATTAGAAGAAAAAAAAGAAAAATTATTAGAGGAGATAAAAATTCTTTTAATACCAAAAGACCCAAATGATGAGAA
AAACGTCATAATGGAGATTAGGGCAGGAGCAGGAGGAGAAGAGGCAGCTCTTTTTGCCCATGACCTTTTTAGGATGTATT
CCATGTATGCAGAGAAAAAAGGTTGGAAAGTAGAAATAATGAGCTCCAATGAAACAGATATCGGAGGATTTAAAGAAGTA
ATTTTGAATATTTCTGGAAAAGGATCTTACAGCAGGTTAAAATACGAAAGTGGTGTTCATAGGGTTCAAAGGGTTCCAAC
CACTGAAGCCGGGGGAAGGATTCACACTTCTACCGCTACAGTGGCAGTTTTGCCTGAAGTCGAGGAAGTTGATGTAGAGA
TAAATCCAAATGATATAAAGGTAGATGTATTTAGGTCAGGCGGTCATGGAGGGCAGAGTGTAAATACGACTGATTCCGCA
GTCAGAGTTACCCATATTCCAACAGGAATTGTCGTAACTTGCCAAGATGAAAGGTCACAGATTCAAAATAGAGAAAGAGC
TCTTAAAATTTTGCGAGCAAAGCTTTATGAAATGGCTCTACAAGAACAACAAAGAGAAATTGCTGAGACACGGAAATCCC
AAGTAGGTACTGGGGAAAGGAGCGAGAGGATAAGAACTTACAATTATCCACAAGGTAGAGTCACTGACCACAGAATAGGT
TTAACTTTATATAGATTACAGGAAGTTCTTGATGGGGACTTGGATGAAATTATAGATGCTTTAATTTTAAATGACCAGGC
AGAAAAACTTAAGAATATGAATCTAAATTAA

Upstream 100 bases:

>100_bases
AAAGGATTTATCGGGAATTGATAGAGTTGTAATAGCAAGGTATAAGTTATAGTTTTCACAAATAGCAATATATGATATAA
TCAATCTTTGAGGTGAGATA

Downstream 100 bases:

>100_bases
AGGAACAGGGAAACCTGTTCTTTTTTATTCCTTAAAAGGATATGAAGTGTGTATTATCAAAAAATATAGGTATAATTTAA
ATTTTTGTGCTTGACTAAAT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MIDKLQAIEDRYVELSQKISDPNIISNVNEWRKYVKEHAAIEDIVLKYREYKKVLEDIEATKELLSSNDEELKEMAEEEL
SQLEEKKEKLLEEIKILLIPKDPNDEKNVIMEIRAGAGGEEAALFAHDLFRMYSMYAEKKGWKVEIMSSNETDIGGFKEV
ILNISGKGSYSRLKYESGVHRVQRVPTTEAGGRIHTSTATVAVLPEVEEVDVEINPNDIKVDVFRSGGHGGQSVNTTDSA
VRVTHIPTGIVVTCQDERSQIQNRERALKILRAKLYEMALQEQQREIAETRKSQVGTGERSERIRTYNYPQGRVTDHRIG
LTLYRLQEVLDGDLDEIIDALILNDQAEKLKNMNLN

Sequences:

>Translated_356_residues
MIDKLQAIEDRYVELSQKISDPNIISNVNEWRKYVKEHAAIEDIVLKYREYKKVLEDIEATKELLSSNDEELKEMAEEEL
SQLEEKKEKLLEEIKILLIPKDPNDEKNVIMEIRAGAGGEEAALFAHDLFRMYSMYAEKKGWKVEIMSSNETDIGGFKEV
ILNISGKGSYSRLKYESGVHRVQRVPTTEAGGRIHTSTATVAVLPEVEEVDVEINPNDIKVDVFRSGGHGGQSVNTTDSA
VRVTHIPTGIVVTCQDERSQIQNRERALKILRAKLYEMALQEQQREIAETRKSQVGTGERSERIRTYNYPQGRVTDHRIG
LTLYRLQEVLDGDLDEIIDALILNDQAEKLKNMNLN
>Mature_356_residues
MIDKLQAIEDRYVELSQKISDPNIISNVNEWRKYVKEHAAIEDIVLKYREYKKVLEDIEATKELLSSNDEELKEMAEEEL
SQLEEKKEKLLEEIKILLIPKDPNDEKNVIMEIRAGAGGEEAALFAHDLFRMYSMYAEKKGWKVEIMSSNETDIGGFKEV
ILNISGKGSYSRLKYESGVHRVQRVPTTEAGGRIHTSTATVAVLPEVEEVDVEINPNDIKVDVFRSGGHGGQSVNTTDSA
VRVTHIPTGIVVTCQDERSQIQNRERALKILRAKLYEMALQEQQREIAETRKSQVGTGERSERIRTYNYPQGRVTDHRIG
LTLYRLQEVLDGDLDEIIDALILNDQAEKLKNMNLN

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=287, Percent_Identity=47.0383275261324, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI34577120, Length=322, Percent_Identity=42.5465838509317, Blast_Score=262, Evalue=3e-70,
Organism=Homo sapiens, GI166795305, Length=192, Percent_Identity=44.2708333333333, Blast_Score=189, Evalue=4e-48,
Organism=Escherichia coli, GI1787462, Length=355, Percent_Identity=51.2676056338028, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=39.0532544378698, Blast_Score=234, Evalue=7e-63,
Organism=Caenorhabditis elegans, GI17542784, Length=306, Percent_Identity=36.2745098039216, Blast_Score=198, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6321295, Length=337, Percent_Identity=44.213649851632, Blast_Score=268, Evalue=9e-73,
Organism=Drosophila melanogaster, GI19921226, Length=317, Percent_Identity=43.5331230283912, Blast_Score=252, Evalue=3e-67,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40601; Mature: 40601

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDKLQAIEDRYVELSQKISDPNIISNVNEWRKYVKEHAAIEDIVLKYREYKKVLEDIEA
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TKELLSSNDEELKEMAEEELSQLEEKKEKLLEEIKILLIPKDPNDEKNVIMEIRAGAGGE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCEEEEEECCCCCC
EAALFAHDLFRMYSMYAEKKGWKVEIMSSNETDIGGFKEVILNISGKGSYSRLKYESGVH
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHEECCCCCCHHHHHHHHHHH
RVQRVPTTEAGGRIHTSTATVAVLPEVEEVDVEINPNDIKVDVFRSGGHGGQSVNTTDSA
HHHHCCCCCCCCEEEECCEEEEECCCHHHEEEEECCCEEEEEEEECCCCCCCCCCCCCCE
VRVTHIPTGIVVTCQDERSQIQNRERALKILRAKLYEMALQEQQREIAETRKSQVGTGER
EEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
SERIRTYNYPQGRVTDHRIGLTLYRLQEVLDGDLDEIIDALILNDQAEKLKNMNLN
HCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHCCCCC
>Mature Secondary Structure
MIDKLQAIEDRYVELSQKISDPNIISNVNEWRKYVKEHAAIEDIVLKYREYKKVLEDIEA
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TKELLSSNDEELKEMAEEELSQLEEKKEKLLEEIKILLIPKDPNDEKNVIMEIRAGAGGE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCEEEEEECCCCCC
EAALFAHDLFRMYSMYAEKKGWKVEIMSSNETDIGGFKEVILNISGKGSYSRLKYESGVH
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHEECCCCCCHHHHHHHHHHH
RVQRVPTTEAGGRIHTSTATVAVLPEVEEVDVEINPNDIKVDVFRSGGHGGQSVNTTDSA
HHHHCCCCCCCCEEEECCEEEEECCCHHHEEEEECCCEEEEEEEECCCCCCCCCCCCCCE
VRVTHIPTGIVVTCQDERSQIQNRERALKILRAKLYEMALQEQQREIAETRKSQVGTGER
EEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
SERIRTYNYPQGRVTDHRIGLTLYRLQEVLDGDLDEIIDALILNDQAEKLKNMNLN
HCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA