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The map label for this gene is leuA1 [H]

Identifier: 167038689

GI number: 167038689

Start: 16644

End: 17840

Strand: Direct

Name: leuA1 [H]

Synonym: Teth514_0014

Alternate gene names: 167038689

Gene position: 16644-17840 (Clockwise)

Preceding gene: 167038688

Following gene: 167038690

Centisome position: 0.68

GC content: 36.93

Gene sequence:

>1197_bases
ATGGGGGTCAAAAGAGTCATTGTTTTTGACACTACGTTAAGGGATGGTGAGCAGACTCCTGGTGTGAATTTTAACAGCAA
TGACAAGTTTGAAATAGCAAAGCAATTGGTAAATTTAGGGGTAGATGTCATAGAAGCTGGATTTCCCGCGGCTTCAAATG
GAGATTTTGAAGCAGTCAAAAATATTGCTGACAAATTAAGAGGGGTTACTATTGCTGCAATGGCAAGGTCTGTAAAAGAG
GATATAGACAGAGCTAGCAGCGCTTTGAAAAATGCTGAAAGGTCGAGATTACACGTTTTTATCGCAACTTCTGATATACA
TCTTAAATACAAACTTAAGATGTCAAGAGAAGAAATGCTTGAAAAAGCCGTTGAAATGGTAAAATACGCAAAAGGAAAAT
TTGATGAGATTCAATTTTCAGCAGAAGACGCTTCAAGGACTGATTGGGATTTCTTAGTAAAAGTTTTTAGTGAGGTAATT
GATGCAGGAGCAAATGTAATAAATGTGCCCGATACTGTGGGATATGCGATGCCAAGAGAATTTGGAGAACTCATAAAATA
CATCAGAAATAATGTTCCCAACATCGATGGAGTAATGATAAGTGCTCATTGTCACAATGATTTAGGAATGGCTGTAGCGA
ATTCTTTGTCTGCCATAGAAAACGGAGCAACTCAAGTAGAAGTGACTGTAAATGGAATAGGAGAGAGGGCAGGAAATGCA
GCCATGGAAGAAGTAGTAATGGCTTTAAATACTAGAAAGGATTATTTTGGCCTTGTTCACGGTATAAACACCAAAGAGAT
ATACAATACAAGCAAATTGGTAAGCGAACTTACAGGAATTAAACTCCAACCTAATAAAGCAATTGTAGGTGCTAATGCTT
TTAGACATCAAGCGGGAATTCACCAACATGGAGTTATAAACAACAGAGCTACTTATGAAATAATGAAACCAGAGGACATT
GGAATAATTCCAGATACTTTTGCATTAGGTAAGCTTTCAGGAAGAAATGCTTTTGAATTGAAAGTCAGACAATTGGGGTA
CAATAATCTTTCACCAGGAGAATTAAGTGATGCTTTTAGAAGGTTTAAAGATTTGGCAGACAGAAAAAAGGTCATTATAG
ATGAGGATATAAGATTTGTGGTAGAGGAGACATTAGAAGAATTTAGAAGTTTTAAGGAGGGAGAAGCTTGGGCTTAA

Upstream 100 bases:

>100_bases
AAAAGATAAAGAGGCAAATCATCTTATTGAAAAAGTCGGCAAAGGTTTGAGAGAAATGATGGCTTGGATTAAAAAAGAAT
GAATAAGGAGGTAGAAGACA

Downstream 100 bases:

>100_bases
CATTGACACAAAAGATATTGTCAGCAAAAATTGGTAGAGAGGTCAAAGCAGGAGAGTTAATAGAAGTAGATGTAGATATG
GTGTTGGGCAATGACGTCAC

Product: pyruvate carboxyltransferase

Products: NA

Alternate protein names: Alpha-IPM synthase 1; Alpha-isopropylmalate synthase 1 [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MGVKRVIVFDTTLRDGEQTPGVNFNSNDKFEIAKQLVNLGVDVIEAGFPAASNGDFEAVKNIADKLRGVTIAAMARSVKE
DIDRASSALKNAERSRLHVFIATSDIHLKYKLKMSREEMLEKAVEMVKYAKGKFDEIQFSAEDASRTDWDFLVKVFSEVI
DAGANVINVPDTVGYAMPREFGELIKYIRNNVPNIDGVMISAHCHNDLGMAVANSLSAIENGATQVEVTVNGIGERAGNA
AMEEVVMALNTRKDYFGLVHGINTKEIYNTSKLVSELTGIKLQPNKAIVGANAFRHQAGIHQHGVINNRATYEIMKPEDI
GIIPDTFALGKLSGRNAFELKVRQLGYNNLSPGELSDAFRRFKDLADRKKVIIDEDIRFVVEETLEEFRSFKEGEAWA

Sequences:

>Translated_398_residues
MGVKRVIVFDTTLRDGEQTPGVNFNSNDKFEIAKQLVNLGVDVIEAGFPAASNGDFEAVKNIADKLRGVTIAAMARSVKE
DIDRASSALKNAERSRLHVFIATSDIHLKYKLKMSREEMLEKAVEMVKYAKGKFDEIQFSAEDASRTDWDFLVKVFSEVI
DAGANVINVPDTVGYAMPREFGELIKYIRNNVPNIDGVMISAHCHNDLGMAVANSLSAIENGATQVEVTVNGIGERAGNA
AMEEVVMALNTRKDYFGLVHGINTKEIYNTSKLVSELTGIKLQPNKAIVGANAFRHQAGIHQHGVINNRATYEIMKPEDI
GIIPDTFALGKLSGRNAFELKVRQLGYNNLSPGELSDAFRRFKDLADRKKVIIDEDIRFVVEETLEEFRSFKEGEAWA
>Mature_397_residues
GVKRVIVFDTTLRDGEQTPGVNFNSNDKFEIAKQLVNLGVDVIEAGFPAASNGDFEAVKNIADKLRGVTIAAMARSVKED
IDRASSALKNAERSRLHVFIATSDIHLKYKLKMSREEMLEKAVEMVKYAKGKFDEIQFSAEDASRTDWDFLVKVFSEVID
AGANVINVPDTVGYAMPREFGELIKYIRNNVPNIDGVMISAHCHNDLGMAVANSLSAIENGATQVEVTVNGIGERAGNAA
MEEVVMALNTRKDYFGLVHGINTKEIYNTSKLVSELTGIKLQPNKAIVGANAFRHQAGIHQHGVINNRATYEIMKPEDIG
IIPDTFALGKLSGRNAFELKVRQLGYNNLSPGELSDAFRRFKDLADRKKVIIDEDIRFVVEETLEEFRSFKEGEAWA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=391, Percent_Identity=46.5473145780051, Blast_Score=355, Evalue=4e-99,
Organism=Saccharomyces cerevisiae, GI6320071, Length=395, Percent_Identity=31.8987341772152, Blast_Score=173, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6320019, Length=376, Percent_Identity=32.4468085106383, Blast_Score=173, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6324682, Length=348, Percent_Identity=31.3218390804598, Blast_Score=136, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6324225, Length=348, Percent_Identity=30.4597701149425, Blast_Score=127, Evalue=3e-30,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 44090; Mature: 43958

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVKRVIVFDTTLRDGEQTPGVNFNSNDKFEIAKQLVNLGVDVIEAGFPAASNGDFEAVK
CCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHH
NIADKLRGVTIAAMARSVKEDIDRASSALKNAERSRLHVFIATSDIHLKYKLKMSREEML
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEEEECCCHHHHH
EKAVEMVKYAKGKFDEIQFSAEDASRTDWDFLVKVFSEVIDAGANVINVPDTVGYAMPRE
HHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEECCCHHHHHCHHH
FGELIKYIRNNVPNIDGVMISAHCHNDLGMAVANSLSAIENGATQVEVTVNGIGERAGNA
HHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHH
AMEEVVMALNTRKDYFGLVHGINTKEIYNTSKLVSELTGIKLQPNKAIVGANAFRHQAGI
HHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCEECCCCEEECCHHHHHHCCC
HQHGVINNRATYEIMKPEDIGIIPDTFALGKLSGRNAFELKVRQLGYNNLSPGELSDAFR
CCCCCCCCCCEEEEECCCCCCCCCCCHHHCCCCCCCCEEEEHHHCCCCCCCCHHHHHHHH
RFKDLADRKKVIIDEDIRFVVEETLEEFRSFKEGEAWA
HHHHHHHHCCEEECHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GVKRVIVFDTTLRDGEQTPGVNFNSNDKFEIAKQLVNLGVDVIEAGFPAASNGDFEAVK
CCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHH
NIADKLRGVTIAAMARSVKEDIDRASSALKNAERSRLHVFIATSDIHLKYKLKMSREEML
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEEEECCCHHHHH
EKAVEMVKYAKGKFDEIQFSAEDASRTDWDFLVKVFSEVIDAGANVINVPDTVGYAMPRE
HHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEECCCHHHHHCHHH
FGELIKYIRNNVPNIDGVMISAHCHNDLGMAVANSLSAIENGATQVEVTVNGIGERAGNA
HHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHH
AMEEVVMALNTRKDYFGLVHGINTKEIYNTSKLVSELTGIKLQPNKAIVGANAFRHQAGI
HHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCEECCCCEEECCHHHHHHCCC
HQHGVINNRATYEIMKPEDIGIIPDTFALGKLSGRNAFELKVRQLGYNNLSPGELSDAFR
CCCCCCCCCCEEEEECCCCCCCCCCCHHHCCCCCCCCEEEEHHHCCCCCCCCHHHHHHHH
RFKDLADRKKVIIDEDIRFVVEETLEEFRSFKEGEAWA
HHHHHHHHCCEEECHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]