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The map label for this gene is leuC

Identifier: 167038690

GI number: 167038690

Start: 17831

End: 19087

Strand: Direct

Name: leuC

Synonym: Teth514_0015

Alternate gene names: 167038690

Gene position: 17831-19087 (Clockwise)

Preceding gene: 167038689

Following gene: 167038691

Centisome position: 0.73

GC content: 39.62

Gene sequence:

>1257_bases
TTGGGCTTAACATTGACACAAAAGATATTGTCAGCAAAAATTGGTAGAGAGGTCAAAGCAGGAGAGTTAATAGAAGTAGA
TGTAGATATGGTGTTGGGCAATGACGTCACTGCTCCTGTTGCTATAAAGGAATTTGAGAAAATAGGAAAAGAAGAGGTGT
TTGACAAGACTAAAATTGCCCTTGTTCCTGACCATTTTGTTCCTAATAAGGACATAAAATCGGCAGAGCAAGTGAATATA
ATGAGAAAATTTGCAAAAAAACATGGAATAGTGAATTTCTTTGAAGTAGGGCAAATGGGGATAGAGCACGCTCTTTTACC
CGAGAAAGGACTTGTACTTCCCGGGGATGTGGTGATAGGTGCCGATTCTCATACATGCACTTATGGCGCTCTTACCTGTT
TTTCTACAGGTGTAGGAAGTACTGACATGGCGGCAGCAATGGCTACTGGCAAGGCATGGTTTAAAGTACCGGAAGCTATA
AAATTTGTATTAAAAGGCAATTTACAAAAATGGGTAAGTGGAAAAGATGTTATCTTGTATATAATCGGAAAAATAGGAGT
TGACGGAGCTTTATACAAATCTATGGAATTTACAGGGAATATAAAAGCTTTGTCAATCGATGATAGATTCACAATTGCCA
ATATGGCAATAGAAGCAGGGGCTAAAAACGGTATTTTCGATTTTGACGAAATTACCGAGGCATATGTTAAGGGGAGAGCA
AAAAGAGAATATAAAGTGTTTGAAAGAGATGAAGACGCCGAGTACAGTGAAGTTTATGAGATAAATTTAGATGAGATAAG
ACCTCAAGTAGCATTTCCTCATTTGCCTGAAAACACTAGAAGCATTGATGAGGTTGGAAAAGTGAAAATAGATCAGGTAG
TTATTGGCTCTTGTACGAATGGACGTCTCTCAGATATGGAGATAGCGTATAAGATACTAAAAGGCAAAAAGGTTCATCCT
GATGTAAGACTTTTAATTTTCCCTGCCACACAGGAAATATACCTTGAATGTGTCAAAAGAGGTTACATTGAAGAGTTTAT
AAAAGCAGGCGCCGCAGTTTCTACTCCTACTTGTGGACCTTGCCTTGGCGGGCACATGGGAATTTTGGCTAGAGGAGAAA
GGGCGTTGGCAACTACTAACAGAAATTTTGTAGGAAGAATGGGACATCCTGAAAGCGAAGTGTATCTTTCAAGTCCAGCT
GTTGCGGCTGCTTCGGCTATTGCAGGATATATCGTAAGTCCAGAGGAGGTGGAATGA

Upstream 100 bases:

>100_bases
AAGATTTGGCAGACAGAAAAAAGGTCATTATAGATGAGGATATAAGATTTGTGGTAGAGGAGACATTAGAAGAATTTAGA
AGTTTTAAGGAGGGAGAAGC

Downstream 100 bases:

>100_bases
TGCAAGGGAAAGCCATAAAATATGGGGACAATATAGATACAGACGTTATAATACCAGCGAGGTTTTTAAACACTTCAGAT
CCACAGGAATTGGCACAGCA

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MGLTLTQKILSAKIGREVKAGELIEVDVDMVLGNDVTAPVAIKEFEKIGKEEVFDKTKIALVPDHFVPNKDIKSAEQVNI
MRKFAKKHGIVNFFEVGQMGIEHALLPEKGLVLPGDVVIGADSHTCTYGALTCFSTGVGSTDMAAAMATGKAWFKVPEAI
KFVLKGNLQKWVSGKDVILYIIGKIGVDGALYKSMEFTGNIKALSIDDRFTIANMAIEAGAKNGIFDFDEITEAYVKGRA
KREYKVFERDEDAEYSEVYEINLDEIRPQVAFPHLPENTRSIDEVGKVKIDQVVIGSCTNGRLSDMEIAYKILKGKKVHP
DVRLLIFPATQEIYLECVKRGYIEEFIKAGAAVSTPTCGPCLGGHMGILARGERALATTNRNFVGRMGHPESEVYLSSPA
VAAASAIAGYIVSPEEVE

Sequences:

>Translated_418_residues
MGLTLTQKILSAKIGREVKAGELIEVDVDMVLGNDVTAPVAIKEFEKIGKEEVFDKTKIALVPDHFVPNKDIKSAEQVNI
MRKFAKKHGIVNFFEVGQMGIEHALLPEKGLVLPGDVVIGADSHTCTYGALTCFSTGVGSTDMAAAMATGKAWFKVPEAI
KFVLKGNLQKWVSGKDVILYIIGKIGVDGALYKSMEFTGNIKALSIDDRFTIANMAIEAGAKNGIFDFDEITEAYVKGRA
KREYKVFERDEDAEYSEVYEINLDEIRPQVAFPHLPENTRSIDEVGKVKIDQVVIGSCTNGRLSDMEIAYKILKGKKVHP
DVRLLIFPATQEIYLECVKRGYIEEFIKAGAAVSTPTCGPCLGGHMGILARGERALATTNRNFVGRMGHPESEVYLSSPA
VAAASAIAGYIVSPEEVE
>Mature_417_residues
GLTLTQKILSAKIGREVKAGELIEVDVDMVLGNDVTAPVAIKEFEKIGKEEVFDKTKIALVPDHFVPNKDIKSAEQVNIM
RKFAKKHGIVNFFEVGQMGIEHALLPEKGLVLPGDVVIGADSHTCTYGALTCFSTGVGSTDMAAAMATGKAWFKVPEAIK
FVLKGNLQKWVSGKDVILYIIGKIGVDGALYKSMEFTGNIKALSIDDRFTIANMAIEAGAKNGIFDFDEITEAYVKGRAK
REYKVFERDEDAEYSEVYEINLDEIRPQVAFPHLPENTRSIDEVGKVKIDQVVIGSCTNGRLSDMEIAYKILKGKKVHPD
VRLLIFPATQEIYLECVKRGYIEEFIKAGAAVSTPTCGPCLGGHMGILARGERALATTNRNFVGRMGHPESEVYLSSPAV
AAASAIAGYIVSPEEVE

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=454, Percent_Identity=30.3964757709251, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI41352693, Length=383, Percent_Identity=26.6318537859008, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI8659555, Length=404, Percent_Identity=24.2574257425743, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1786259, Length=463, Percent_Identity=31.9654427645788, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI87081781, Length=348, Percent_Identity=26.7241379310345, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI2367097, Length=462, Percent_Identity=26.8398268398268, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787531, Length=361, Percent_Identity=26.0387811634349, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25149337, Length=448, Percent_Identity=29.4642857142857, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI32564738, Length=366, Percent_Identity=31.4207650273224, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI25149342, Length=298, Percent_Identity=30.2013422818792, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=395, Percent_Identity=24.8101265822785, Blast_Score=78, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6320440, Length=450, Percent_Identity=34.2222222222222, Blast_Score=229, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6321429, Length=475, Percent_Identity=31.5789473684211, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6323335, Length=448, Percent_Identity=29.6875, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6322261, Length=458, Percent_Identity=27.292576419214, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI281365315, Length=449, Percent_Identity=29.6213808463252, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI17864292, Length=449, Percent_Identity=29.6213808463252, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI161076999, Length=412, Percent_Identity=30.3398058252427, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI28571643, Length=449, Percent_Identity=30.0668151447661, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI17137564, Length=386, Percent_Identity=25.1295336787565, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24645686, Length=377, Percent_Identity=24.9336870026525, Blast_Score=82, Evalue=7e-16,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_THEPX (B0K0Y2)

Other databases:

- EMBL:   CP000923
- RefSeq:   YP_001661675.1
- ProteinModelPortal:   B0K0Y2
- SMR:   B0K0Y2
- GeneID:   5876698
- GenomeReviews:   CP000923_GR
- KEGG:   tex:Teth514_0015
- HOGENOM:   HBG330745
- OMA:   PENTKTI
- ProtClustDB:   PRK00402
- BioCyc:   TSP1755:TETH514_0015-MONOMER
- HAMAP:   MF_01027
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- InterPro:   IPR011823
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR01343
- TIGRFAMs:   TIGR02086
- TIGRFAMs:   TIGR02083

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 45597; Mature: 45466

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLTLTQKILSAKIGREVKAGELIEVDVDMVLGNDVTAPVAIKEFEKIGKEEVFDKTKIA
CCCHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHCCHHHHHCCCEEE
LVPDHFVPNKDIKSAEQVNIMRKFAKKHGIVNFFEVGQMGIEHALLPEKGLVLPGDVVIG
EECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHHCHHHHCCCCCCEECCCCEEEE
ADSHTCTYGALTCFSTGVGSTDMAAAMATGKAWFKVPEAIKFVLKGNLQKWVSGKDVILY
CCCCCEEHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHCCCCCEEEE
IIGKIGVDGALYKSMEFTGNIKALSIDDRFTIANMAIEAGAKNGIFDFDEITEAYVKGRA
EEEHHCCCHHHHHCEEECCCEEEEEECCCEEEEHHHHCCCCCCCCCCHHHHHHHHHCCCC
KREYKVFERDEDAEYSEVYEINLDEIRPQVAFPHLPENTRSIDEVGKVKIDQVVIGSCTN
CCCCCHHCCCCCCCCHHEEEECHHHCCCCCCCCCCCCCCCCHHHHCCEEEEEEEEECCCC
GRLSDMEIAYKILKGKKVHPDVRLLIFPATQEIYLECVKRGYIEEFIKAGAAVSTPTCGP
CCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
CLGGHMGILARGERALATTNRNFVGRMGHPESEVYLSSPAVAAASAIAGYIVSPEEVE
CCCCCCCEEECCCEEEEECCCCCEECCCCCCCCEEECCCHHHHHHHHHHEECCHHCCC
>Mature Secondary Structure 
GLTLTQKILSAKIGREVKAGELIEVDVDMVLGNDVTAPVAIKEFEKIGKEEVFDKTKIA
CCHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHCCHHHHHCCCEEE
LVPDHFVPNKDIKSAEQVNIMRKFAKKHGIVNFFEVGQMGIEHALLPEKGLVLPGDVVIG
EECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHHCHHHHCCCCCCEECCCCEEEE
ADSHTCTYGALTCFSTGVGSTDMAAAMATGKAWFKVPEAIKFVLKGNLQKWVSGKDVILY
CCCCCEEHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHCCCCCEEEE
IIGKIGVDGALYKSMEFTGNIKALSIDDRFTIANMAIEAGAKNGIFDFDEITEAYVKGRA
EEEHHCCCHHHHHCEEECCCEEEEEECCCEEEEHHHHCCCCCCCCCCHHHHHHHHHCCCC
KREYKVFERDEDAEYSEVYEINLDEIRPQVAFPHLPENTRSIDEVGKVKIDQVVIGSCTN
CCCCCHHCCCCCCCCHHEEEECHHHCCCCCCCCCCCCCCCCHHHHCCEEEEEEEEECCCC
GRLSDMEIAYKILKGKKVHPDVRLLIFPATQEIYLECVKRGYIEEFIKAGAAVSTPTCGP
CCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
CLGGHMGILARGERALATTNRNFVGRMGHPESEVYLSSPAVAAASAIAGYIVSPEEVE
CCCCCCCEEECCCEEEEECCCCCEECCCCCCCCEEECCCHHHHHHHHHHEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA