Click here to switch to the map view.

The map label for this gene is hisJ [H]

Identifier: 163844780

GI number: 163844780

Start: 612612

End: 613373

Strand: Reverse

Name: hisJ [H]

Synonym: BSUIS_B0627

Alternate gene names: 163844780

Gene position: 613373-612612 (Counterclockwise)

Preceding gene: 163844781

Following gene: 163844779

Centisome position: 43.79

GC content: 55.12

Gene sequence:

>762_bases
ATGCTAAAGGCCATAGTATTCGCCGGCATTGCATCCGTCATTGCCGTCCTTCCGGCACAGGCCAGGGAGTGGAAGGAAAT
CCGCATCGCGACCGAAGGTGCTTACCCGCCCTTCAATTTCGTTGCTTCCGATGGCTCGCTTCAGGGGCTGGATGTCGATA
TCGCCAAGGCGCTTTGCAAGGCGATGGACGCGAAATGTACGATTGTCGCCAATGACTGGGACGGCATGATTCCGGGCCTG
AAGTCGAACAAGTTCGATGCGGTCATCGCTTCCATGAGCGTCACGGAAGAGCGCAAGAAGGAAGTCGCGTTCACCGATAA
ATATTATTCCACCCCGCTATCGGTCGCCGTACCGAAGGATAGCGACATTACATCCGTCGCGCCGGAATCCTTCAAGGGCA
AGACCGTGGGCGCCCAAGGCTCCACCACGCAGGGCAATTACGCCGAAGATGTCTATGGCAAGGCCGGTGCGGAGGTGAAA
CTTTACCCGACTGCCGACGAGGCCAATGCCGACCTGAAAAGCGGCCGTCTTGACGCTGTCGTTTCCGATAAATTTCCGAT
TTCCGACTGGATCAAGGGTGATGGCGCGGATTGCTGCAAGCTGATCGGCGATATTCCGGGCACGCAGACCGATACCGCAG
TTGCTTTGCGTCTGGGCGATGATGATCTGCGCGAACAGTTTAATGCGGCGATAAAGAAAATCCGCGAAGACGGCACCTAT
GCCACAATCGTGAAGAAATATTTCGATTTCGATATCTATTGA

Upstream 100 bases:

>100_bases
GGTGCTCTCTTAAAAAGGGGACGCGCAAAATGGGGCGCTGTTCCTTGGCAGGCATTAAGCCTGAACAATACAACGATAAT
AAACAATAAAGGGAACTATC

Downstream 100 bases:

>100_bases
TCGCTTGTAAAACTATCAACTCTTTGCTGAAAACAGCAACGCGCCGGTCTTCTTCGGCGCGTTTATTTCTTTGTTCTAGG
AATTGGAAATGCCAATGCAG

Product: hypothetical protein

Products: ADP; phosphate; L-histidine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MLKAIVFAGIASVIAVLPAQAREWKEIRIATEGAYPPFNFVASDGSLQGLDVDIAKALCKAMDAKCTIVANDWDGMIPGL
KSNKFDAVIASMSVTEERKKEVAFTDKYYSTPLSVAVPKDSDITSVAPESFKGKTVGAQGSTTQGNYAEDVYGKAGAEVK
LYPTADEANADLKSGRLDAVVSDKFPISDWIKGDGADCCKLIGDIPGTQTDTAVALRLGDDDLREQFNAAIKKIREDGTY
ATIVKKYFDFDIY

Sequences:

>Translated_253_residues
MLKAIVFAGIASVIAVLPAQAREWKEIRIATEGAYPPFNFVASDGSLQGLDVDIAKALCKAMDAKCTIVANDWDGMIPGL
KSNKFDAVIASMSVTEERKKEVAFTDKYYSTPLSVAVPKDSDITSVAPESFKGKTVGAQGSTTQGNYAEDVYGKAGAEVK
LYPTADEANADLKSGRLDAVVSDKFPISDWIKGDGADCCKLIGDIPGTQTDTAVALRLGDDDLREQFNAAIKKIREDGTY
ATIVKKYFDFDIY
>Mature_253_residues
MLKAIVFAGIASVIAVLPAQAREWKEIRIATEGAYPPFNFVASDGSLQGLDVDIAKALCKAMDAKCTIVANDWDGMIPGL
KSNKFDAVIASMSVTEERKKEVAFTDKYYSTPLSVAVPKDSDITSVAPESFKGKTVGAQGSTTQGNYAEDVYGKAGAEVK
LYPTADEANADLKSGRLDAVVSDKFPISDWIKGDGADCCKLIGDIPGTQTDTAVALRLGDDDLREQFNAAIKKIREDGTY
ATIVKKYFDFDIY

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788648, Length=236, Percent_Identity=40.2542372881356, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1788649, Length=248, Percent_Identity=37.9032258064516, Blast_Score=158, Evalue=3e-40,
Organism=Escherichia coli, GI1787088, Length=247, Percent_Identity=37.246963562753, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1787085, Length=243, Percent_Identity=34.156378600823, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1788228, Length=237, Percent_Identity=31.6455696202532, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1787031, Length=226, Percent_Identity=34.070796460177, Blast_Score=117, Evalue=8e-28,

Paralogues:

None

Copy number: 3400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27219; Mature: 27219

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKAIVFAGIASVIAVLPAQAREWKEIRIATEGAYPPFNFVASDGSLQGLDVDIAKALCK
CCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCHHHHHHHHH
AMDAKCTIVANDWDGMIPGLKSNKFDAVIASMSVTEERKKEVAFTDKYYSTPLSVAVPKD
HCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCEECHHHHCCCEEEEECCC
SDITSVAPESFKGKTVGAQGSTTQGNYAEDVYGKAGAEVKLYPTADEANADLKSGRLDAV
CCCCCCCCCCCCCCEECCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEE
VSDKFPISDWIKGDGADCCKLIGDIPGTQTDTAVALRLGDDDLREQFNAAIKKIREDGTY
ECCCCCCHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCH
ATIVKKYFDFDIY
HHHHHHHHCCCCC
>Mature Secondary Structure
MLKAIVFAGIASVIAVLPAQAREWKEIRIATEGAYPPFNFVASDGSLQGLDVDIAKALCK
CCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCHHHHHHHHH
AMDAKCTIVANDWDGMIPGLKSNKFDAVIASMSVTEERKKEVAFTDKYYSTPLSVAVPKD
HCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCEECHHHHCCCEEEEECCC
SDITSVAPESFKGKTVGAQGSTTQGNYAEDVYGKAGAEVKLYPTADEANADLKSGRLDAV
CCCCCCCCCCCCCCEECCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEE
VSDKFPISDWIKGDGADCCKLIGDIPGTQTDTAVALRLGDDDLREQFNAAIKKIREDGTY
ECCCCCCHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCH
ATIVKKYFDFDIY
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]