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The map label for this gene is argT [H]

Identifier: 163844781

GI number: 163844781

Start: 613529

End: 614302

Strand: Reverse

Name: argT [H]

Synonym: BSUIS_B0628

Alternate gene names: 163844781

Gene position: 614302-613529 (Counterclockwise)

Preceding gene: 163844782

Following gene: 163844780

Centisome position: 43.85

GC content: 56.2

Gene sequence:

>774_bases
ATGTTGAAATCACTCGTATTGGCCGGTCTGGCCTCTGTCGTTCTCACCGTGTCCGTTCAGGCTCCGGTTCAGGCAAAGGA
ATGGAAAGAAATCCGCATTGCCAGCGAAGGCGCTTATCCGCCCTTCAACTATATGTCGCCGGACGGCAAGCTGGTGGGCT
TCGATCTCGATATCGCCAATGCGCTGTGCGAAGCAATGGAAGCCAAGTGCACGATCGTCGCCAATGACTGGGACGGTATG
ATCCCCAGCCTTCAGGCCAACAAGTTCGACGCCGTGATTGCCTCGATGGCGATTACCGATGAACGCAAGAAGCAGGTTGC
CTTCACCGACCGTTATTACACGACGCCGCTTGCCGTCGTGGTTCCGAAGGATTCGGATATCGTCTCGCTTGAGCCTTCCG
CATTTGCAGGCAAGATGGTCGGTGCGCAGGCTGGCACCACGCAGGGCAATTATGCCGATGACGTTTACGGCAAGGCGGGC
GCGGACGTGAAGCTCTACCCGACCGCTGACGAAGCCAATGCCGACCTCGAAAGCGGCCGCCTCGACGCCCTTGCCGCCGA
CAAGTTCATTGCCGCTGACTGGCTGAAAAAGCAGGGTGCAGATTGCTGCAAGTTCCTTGGTGATATTCCGGGTTCTGAAA
CGCAGATTGCGGTCGCATTGCGTCAGGGCGACGATGATCTGCGCGAGCAGTTCAATGCGGCGATCAAGAAAATCCGCGAA
AACGGCACCTACGAAACGATCCGCAAGAAGTATTTCGATTTCGATATCTATTGA

Upstream 100 bases:

>100_bases
TACCAAAGTCGCAATTGAAACGGGCAGGGTTTGAGCGCATTGCCCGATCAAACCAGCGGTTGGGGGCATCCCCGCTGACT
CTAATGCAAACAGGGAAATC

Downstream 100 bases:

>100_bases
TGCAATAAAATTGGCATCGGGCGCCCGCAAAACTTCCTTGAAGTCCGGGGCGTCCGGTGCTCTCTTAAAAAGGGGACGCG
CAAAATGGGGCGCTGTTCCT

Product: hypothetical protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLKSLVLAGLASVVLTVSVQAPVQAKEWKEIRIASEGAYPPFNYMSPDGKLVGFDLDIANALCEAMEAKCTIVANDWDGM
IPSLQANKFDAVIASMAITDERKKQVAFTDRYYTTPLAVVVPKDSDIVSLEPSAFAGKMVGAQAGTTQGNYADDVYGKAG
ADVKLYPTADEANADLESGRLDALAADKFIAADWLKKQGADCCKFLGDIPGSETQIAVALRQGDDDLREQFNAAIKKIRE
NGTYETIRKKYFDFDIY

Sequences:

>Translated_257_residues
MLKSLVLAGLASVVLTVSVQAPVQAKEWKEIRIASEGAYPPFNYMSPDGKLVGFDLDIANALCEAMEAKCTIVANDWDGM
IPSLQANKFDAVIASMAITDERKKQVAFTDRYYTTPLAVVVPKDSDIVSLEPSAFAGKMVGAQAGTTQGNYADDVYGKAG
ADVKLYPTADEANADLESGRLDALAADKFIAADWLKKQGADCCKFLGDIPGSETQIAVALRQGDDDLREQFNAAIKKIRE
NGTYETIRKKYFDFDIY
>Mature_257_residues
MLKSLVLAGLASVVLTVSVQAPVQAKEWKEIRIASEGAYPPFNYMSPDGKLVGFDLDIANALCEAMEAKCTIVANDWDGM
IPSLQANKFDAVIASMAITDERKKQVAFTDRYYTTPLAVVVPKDSDIVSLEPSAFAGKMVGAQAGTTQGNYADDVYGKAG
ADVKLYPTADEANADLESGRLDALAADKFIAADWLKKQGADCCKFLGDIPGSETQIAVALRQGDDDLREQFNAAIKKIRE
NGTYETIRKKYFDFDIY

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=269, Percent_Identity=37.1747211895911, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1788648, Length=237, Percent_Identity=39.662447257384, Blast_Score=170, Evalue=9e-44,
Organism=Escherichia coli, GI1787088, Length=228, Percent_Identity=36.8421052631579, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1787085, Length=256, Percent_Identity=34.375, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1788228, Length=234, Percent_Identity=32.4786324786325, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1787031, Length=253, Percent_Identity=33.201581027668, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1786876, Length=232, Percent_Identity=24.5689655172414, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27904; Mature: 27904

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKSLVLAGLASVVLTVSVQAPVQAKEWKEIRIASEGAYPPFNYMSPDGKLVGFDLDIAN
CHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEHHHHH
ALCEAMEAKCTIVANDWDGMIPSLQANKFDAVIASMAITDERKKQVAFTDRYYTTPLAVV
HHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHEEEECCEECCCEEEE
VPKDSDIVSLEPSAFAGKMVGAQAGTTQGNYADDVYGKAGADVKLYPTADEANADLESGR
EECCCCEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCC
LDALAADKFIAADWLKKQGADCCKFLGDIPGSETQIAVALRQGDDDLREQFNAAIKKIRE
CCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHH
NGTYETIRKKYFDFDIY
CCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MLKSLVLAGLASVVLTVSVQAPVQAKEWKEIRIASEGAYPPFNYMSPDGKLVGFDLDIAN
CHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEHHHHH
ALCEAMEAKCTIVANDWDGMIPSLQANKFDAVIASMAITDERKKQVAFTDRYYTTPLAVV
HHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHEEEECCEECCCEEEE
VPKDSDIVSLEPSAFAGKMVGAQAGTTQGNYADDVYGKAGADVKLYPTADEANADLESGR
EECCCCEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCC
LDALAADKFIAADWLKKQGADCCKFLGDIPGSETQIAVALRQGDDDLREQFNAAIKKIRE
CCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHH
NGTYETIRKKYFDFDIY
CCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]