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The map label for this gene is lplA2 [H]

Identifier: 162448146

GI number: 162448146

Start: 1365058

End: 1365975

Strand: Direct

Name: lplA2 [H]

Synonym: ACL_1307

Alternate gene names: 162448146

Gene position: 1365058-1365975 (Clockwise)

Preceding gene: 162448125

Following gene: 162448157

Centisome position: 91.19

GC content: 31.59

Gene sequence:

>918_bases
ATGAAAACTATACATGCAATCAGTCAAGATCCTTACTTTAATTTAGCTTGGGAAGAATATATATTTAAACATATCCATAA
AGATGAAGACATCTTTTTGTTGTGGCAAAATGCCCCTTCTATCATAATTGGAAGAAATCAAAATGTATTTGAAGAAGTCT
ATTTAGATTATATTGTTAAACATCATATACCCCTGATACGTAGAAACTCAGGTGGTGGTACAGTCTATCATGATCTAGGT
AATATTAATTTTAGTTTTATGACAAGCGCTAAAGGCACAATCAATAATTATAAGTTGATGACAGAAAAGATCAGGGAAGC
TTTAAAAAAGTTAGGTATACCAGTTGAATTTATCGAAAAGTCAGACTTAAAAGTGAATGGTTTAAAGGTTAGTGGTAATT
CTCAGTTCGTCTTTAAAGATAAACTACTGCATCACGGCACACTACTTTTCGACTCTAATTTAGATACACTAAATAGCGTG
ATTAAACCTAAACATGATGCAATCGATAGTATCGGTGTGAAGTCCAATCGTTCTTTAGTGACAAACTTAAAAGAATATAC
TTCCCTTTCTATTGAAGAATTTAAGGCTTATTTACTCAAAGAAATAAGTGGTGAAAGTGAAGTATTAGAATTATCTACCA
AAGATATTAAGAAAATTGAAATGCTTAAAGAAGAACGTTACTTATCATTTAACTGGAATTATGGACAATCTCCAAAATCC
ACAATTATTAAGTCTTTGGGGGACTACAGTATTAAATTGACTGTGAGTTTTGGTCATGTTGAGGACTGTTTTATTATGCA
TGAAGGTACGGTTGCACTTTCTTTATCATCTAGTTTAGTTGGGGAAAGACTTTTCCCAACCGACTTAAGTTTCTTAAAGA
AAAAAGCACCTGAAGTATATCAGATGCTTTTTGAATAA

Upstream 100 bases:

>100_bases
GTTGTATATCAGCAATATGTTCATAATCTTTTCCCCTGAGTACCTTATAGTTCCATTATATAATATTATTATGATAAGAT
TAATTAAACGAGGTAATAAA

Downstream 100 bases:

>100_bases
GTTATTATGATAAATCGCCTAATACAGGCACTACAACTTTTTCAAATTGGCCAATAATTGGTCCTAATAAGAATGCAGAT
ACTAATGTAAACCAACCAAT

Product: lipoate-protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKTIHAISQDPYFNLAWEEYIFKHIHKDEDIFLLWQNAPSIIIGRNQNVFEEVYLDYIVKHHIPLIRRNSGGGTVYHDLG
NINFSFMTSAKGTINNYKLMTEKIREALKKLGIPVEFIEKSDLKVNGLKVSGNSQFVFKDKLLHHGTLLFDSNLDTLNSV
IKPKHDAIDSIGVKSNRSLVTNLKEYTSLSIEEFKAYLLKEISGESEVLELSTKDIKKIEMLKEERYLSFNWNYGQSPKS
TIIKSLGDYSIKLTVSFGHVEDCFIMHEGTVALSLSSSLVGERLFPTDLSFLKKKAPEVYQMLFE

Sequences:

>Translated_305_residues
MKTIHAISQDPYFNLAWEEYIFKHIHKDEDIFLLWQNAPSIIIGRNQNVFEEVYLDYIVKHHIPLIRRNSGGGTVYHDLG
NINFSFMTSAKGTINNYKLMTEKIREALKKLGIPVEFIEKSDLKVNGLKVSGNSQFVFKDKLLHHGTLLFDSNLDTLNSV
IKPKHDAIDSIGVKSNRSLVTNLKEYTSLSIEEFKAYLLKEISGESEVLELSTKDIKKIEMLKEERYLSFNWNYGQSPKS
TIIKSLGDYSIKLTVSFGHVEDCFIMHEGTVALSLSSSLVGERLFPTDLSFLKKKAPEVYQMLFE
>Mature_305_residues
MKTIHAISQDPYFNLAWEEYIFKHIHKDEDIFLLWQNAPSIIIGRNQNVFEEVYLDYIVKHHIPLIRRNSGGGTVYHDLG
NINFSFMTSAKGTINNYKLMTEKIREALKKLGIPVEFIEKSDLKVNGLKVSGNSQFVFKDKLLHHGTLLFDSNLDTLNSV
IKPKHDAIDSIGVKSNRSLVTNLKEYTSLSIEEFKAYLLKEISGESEVLELSTKDIKKIEMLKEERYLSFNWNYGQSPKS
TIIKSLGDYSIKLTVSFGHVEDCFIMHEGTVALSLSSSLVGERLFPTDLSFLKKKAPEVYQMLFE

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=315, Percent_Identity=30.7936507936508, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI21729884, Length=315, Percent_Identity=30.7936507936508, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI21729882, Length=315, Percent_Identity=30.7936507936508, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI21729880, Length=315, Percent_Identity=30.7936507936508, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI21729878, Length=315, Percent_Identity=30.7936507936508, Blast_Score=132, Evalue=4e-31,
Organism=Escherichia coli, GI1790846, Length=246, Percent_Identity=34.5528455284553, Blast_Score=136, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71981909, Length=241, Percent_Identity=29.8755186721992, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6322415, Length=281, Percent_Identity=28.1138790035587, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28573464, Length=308, Percent_Identity=28.2467532467532, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 34937; Mature: 34937

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIHAISQDPYFNLAWEEYIFKHIHKDEDIFLLWQNAPSIIIGRNQNVFEEVYLDYIVK
CCCCCCCCCCCCEEEHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHH
HHIPLIRRNSGGGTVYHDLGNINFSFMTSAKGTINNYKLMTEKIREALKKLGIPVEFIEK
HCCCEEEECCCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEECCC
SDLKVNGLKVSGNSQFVFKDKLLHHGTLLFDSNLDTLNSVIKPKHDAIDSIGVKSNRSLV
CCCEEEEEEECCCCEEEEECHHHHCCEEEEECCHHHHHHHHCCCHHHHHHCCCCCCCHHH
TNLKEYTSLSIEEFKAYLLKEISGESEVLELSTKDIKKIEMLKEERYLSFNWNYGQSPKS
HHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHCCEEEEECCCCCCHHH
TIIKSLGDYSIKLTVSFGHVEDCFIMHEGTVALSLSSSLVGERLFPTDLSFLKKKAPEVY
HHHHHCCCEEEEEEEEECCCEEEEEEECCEEEEEECHHHHCCCCCCCCHHHHHHCCHHHH
QMLFE
HHHCC
>Mature Secondary Structure
MKTIHAISQDPYFNLAWEEYIFKHIHKDEDIFLLWQNAPSIIIGRNQNVFEEVYLDYIVK
CCCCCCCCCCCCEEEHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHH
HHIPLIRRNSGGGTVYHDLGNINFSFMTSAKGTINNYKLMTEKIREALKKLGIPVEFIEK
HCCCEEEECCCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEECCC
SDLKVNGLKVSGNSQFVFKDKLLHHGTLLFDSNLDTLNSVIKPKHDAIDSIGVKSNRSLV
CCCEEEEEEECCCCEEEEECHHHHCCEEEEECCHHHHHHHHCCCHHHHHHCCCCCCCHHH
TNLKEYTSLSIEEFKAYLLKEISGESEVLELSTKDIKKIEMLKEERYLSFNWNYGQSPKS
HHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHCCEEEEECCCCCCHHH
TIIKSLGDYSIKLTVSFGHVEDCFIMHEGTVALSLSSSLVGERLFPTDLSFLKKKAPEVY
HHHHHCCCEEEEEEEEECCCEEEEEEECCEEEEEECHHHHCCCCCCCCHHHHHHCCHHHH
QMLFE
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]