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The map label for this gene is potD-A [H]

Identifier: 162448125

GI number: 162448125

Start: 1344015

End: 1345154

Strand: Direct

Name: potD-A [H]

Synonym: ACL_1286

Alternate gene names: 162448125

Gene position: 1344015-1345154 (Clockwise)

Preceding gene: 162448124

Following gene: 162448146

Centisome position: 89.78

GC content: 33.77

Gene sequence:

>1140_bases
ATGAAAAAGTTATATATATTACTTGCATCAGTACTTACCATTTTTATTCTAAATGGTTGTACGATAAACAATAAATTATT
AATCCTTAACTGGGGTGAATATATCAACGATGATCTTGTCTTATTATTTGAAGAGACATATGGTGTTGAAGTATCCATCT
CAATTGCAGATAGTAACGAACTATTCTATTCTAAAATCAAATCTGGTACAACAGCATATGACTTAGTACTACCAGGAGAT
TACATGGTAGAAAAATTAATCAAAGAAGATTTGATTCAACAAATCCAATTTGATAAACTCTCTAACTATGACCCAGTAAA
TAACCCTTATATGCATGGTCTACAAAGTCTAATGGCTACCATGTCTGAAGGTAGTGAAAATTATTTTGTACCATACTTTT
GGGGGACATTTGGACTTATGTATAACAACCGAGTTCCCGGCTTAAAAGAAGCCTTAGAAACACATGGTTGGAATGCTTAC
TTCGAACCCAATTTAAGACCTACAAAACGTGTTGGTATGTATGATGTTGCACAATATGCTTATGCATCCAGTTTACTATA
CTTAGGACTAGATCCAAACGCACTACCTGATACAACACCAGATGGTTTTAGTAAAACACACTTAGAACTATCTAGACAAG
CACTCAATCAAGCAGAGTTTACTGTATTTGCTGATGATGCGTTAAAAAAGGATATACAAGCTAACAACTTAGACTTAGCA
TTTGTTTGGACAGGAGACTTCCTTGATATGCTTTATGTGGATTTAGATGAAGGTCTAGATTATGACCAAAAAACATATGA
CATTTACATACCTGACCAAACGATGGCTTTTGTAGATTCATTTGTTATTCCTAAAAAATCAAGACACGTAGATTTAGCTC
ATGAGTTCATCAACTTCTTCTTAAATCCTGACAATGCCTATGAAAATGCTAGTGTTGTAGGGTACTCCACACCGCTTGCG
AATGTATATGATCGTATTGCAAACTATACAGGTGATGATGTTTGGTTAACCAATTGGTCTAGAGCTTATAGTGATTACTA
CCCAAATACAGAAAACTTTAAAGGTATTCCTTATGCAAGTTTTAATCAAACGATTATGGCTCAACTACTCTTAATGTTTA
ACGACGTTAAAACAAATTAA

Upstream 100 bases:

>100_bases
CTTCTGTATATGCCTTTTCTACATTGCTTACATTAATGACTTTATTAAGTCTTACACTCTATCAAGTGTTTATTAAAAAA
GATAAAAAAGGTAAAATAAA

Downstream 100 bases:

>100_bases
AAATAATACCAGTCACTTCAAAATGACTGGTATTTTTATTTATGTTGTAAGATTTGATTTTCTCCAATAAGTTTTATATC
CGGCTTTATCCTTCATATTA

Product: ABC transporter substrate-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MKKLYILLASVLTIFILNGCTINNKLLILNWGEYINDDLVLLFEETYGVEVSISIADSNELFYSKIKSGTTAYDLVLPGD
YMVEKLIKEDLIQQIQFDKLSNYDPVNNPYMHGLQSLMATMSEGSENYFVPYFWGTFGLMYNNRVPGLKEALETHGWNAY
FEPNLRPTKRVGMYDVAQYAYASSLLYLGLDPNALPDTTPDGFSKTHLELSRQALNQAEFTVFADDALKKDIQANNLDLA
FVWTGDFLDMLYVDLDEGLDYDQKTYDIYIPDQTMAFVDSFVIPKKSRHVDLAHEFINFFLNPDNAYENASVVGYSTPLA
NVYDRIANYTGDDVWLTNWSRAYSDYYPNTENFKGIPYASFNQTIMAQLLLMFNDVKTN

Sequences:

>Translated_379_residues
MKKLYILLASVLTIFILNGCTINNKLLILNWGEYINDDLVLLFEETYGVEVSISIADSNELFYSKIKSGTTAYDLVLPGD
YMVEKLIKEDLIQQIQFDKLSNYDPVNNPYMHGLQSLMATMSEGSENYFVPYFWGTFGLMYNNRVPGLKEALETHGWNAY
FEPNLRPTKRVGMYDVAQYAYASSLLYLGLDPNALPDTTPDGFSKTHLELSRQALNQAEFTVFADDALKKDIQANNLDLA
FVWTGDFLDMLYVDLDEGLDYDQKTYDIYIPDQTMAFVDSFVIPKKSRHVDLAHEFINFFLNPDNAYENASVVGYSTPLA
NVYDRIANYTGDDVWLTNWSRAYSDYYPNTENFKGIPYASFNQTIMAQLLLMFNDVKTN
>Mature_379_residues
MKKLYILLASVLTIFILNGCTINNKLLILNWGEYINDDLVLLFEETYGVEVSISIADSNELFYSKIKSGTTAYDLVLPGD
YMVEKLIKEDLIQQIQFDKLSNYDPVNNPYMHGLQSLMATMSEGSENYFVPYFWGTFGLMYNNRVPGLKEALETHGWNAY
FEPNLRPTKRVGMYDVAQYAYASSLLYLGLDPNALPDTTPDGFSKTHLELSRQALNQAEFTVFADDALKKDIQANNLDLA
FVWTGDFLDMLYVDLDEGLDYDQKTYDIYIPDQTMAFVDSFVIPKKSRHVDLAHEFINFFLNPDNAYENASVVGYSTPLA
NVYDRIANYTGDDVWLTNWSRAYSDYYPNTENFKGIPYASFNQTIMAQLLLMFNDVKTN

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=302, Percent_Identity=28.1456953642384, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1787078, Length=316, Percent_Identity=28.1645569620253, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 43399; Mature: 43399

Theoretical pI: Translated: 4.16; Mature: 4.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLYILLASVLTIFILNGCTINNKLLILNWGEYINDDLVLLFEETYGVEVSISIADSNE
CCHHHHHHHHHHHHHHHCCCEECCEEEEEECCCCCCCCEEEEEEECCCEEEEEEEECCCH
LFYSKIKSGTTAYDLVLPGDYMVEKLIKEDLIQQIQFDKLSNYDPVNNPYMHGLQSLMAT
HHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
MSEGSENYFVPYFWGTFGLMYNNRVPGLKEALETHGWNAYFEPNLRPTKRVGMYDVAQYA
HHCCCCCEEEEEEHHCEEEEECCCCCCHHHHHHHCCCCEEECCCCCCCHHCCHHHHHHHH
YASSLLYLGLDPNALPDTTPDGFSKTHLELSRQALNQAEFTVFADDALKKDIQANNLDLA
HHHCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHCCCCCCCEEE
FVWTGDFLDMLYVDLDEGLDYDQKTYDIYIPDQTMAFVDSFVIPKKSRHVDLAHEFINFF
EEECCCCHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHE
LNPDNAYENASVVGYSTPLANVYDRIANYTGDDVWLTNWSRAYSDYYPNTENFKGIPYAS
ECCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCEEEECHHHHHHHCCCCCCCCCCCCCCC
FNQTIMAQLLLMFNDVKTN
CCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKLYILLASVLTIFILNGCTINNKLLILNWGEYINDDLVLLFEETYGVEVSISIADSNE
CCHHHHHHHHHHHHHHHCCCEECCEEEEEECCCCCCCCEEEEEEECCCEEEEEEEECCCH
LFYSKIKSGTTAYDLVLPGDYMVEKLIKEDLIQQIQFDKLSNYDPVNNPYMHGLQSLMAT
HHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
MSEGSENYFVPYFWGTFGLMYNNRVPGLKEALETHGWNAYFEPNLRPTKRVGMYDVAQYA
HHCCCCCEEEEEEHHCEEEEECCCCCCHHHHHHHCCCCEEECCCCCCCHHCCHHHHHHHH
YASSLLYLGLDPNALPDTTPDGFSKTHLELSRQALNQAEFTVFADDALKKDIQANNLDLA
HHHCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHCCCCCCCEEE
FVWTGDFLDMLYVDLDEGLDYDQKTYDIYIPDQTMAFVDSFVIPKKSRHVDLAHEFINFF
EEECCCCHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHE
LNPDNAYENASVVGYSTPLANVYDRIANYTGDDVWLTNWSRAYSDYYPNTENFKGIPYAS
ECCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCEEEECHHHHHHHCCCCCCCCCCCCCCC
FNQTIMAQLLLMFNDVKTN
CCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]