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The map label for this gene is def [C]

Identifier: 162448037

GI number: 162448037

Start: 1245120

End: 1245698

Strand: Direct

Name: def [C]

Synonym: ACL_1194

Alternate gene names: 162448037

Gene position: 1245120-1245698 (Clockwise)

Preceding gene: 162448025

Following gene: 162448042

Centisome position: 83.17

GC content: 36.79

Gene sequence:

>579_bases
ATGAACCATATCATTAGGGAAGGACACCCTAGTTTAACAACCCCATCAAAACCCGTCCTATTACCATTATCTCAAAAAGA
TATAGATTTAGGTAAATCTCTAATAGAATATGTGAAAAATAGTCAAGATGATGCAATGGTTAAAAAACATCGCCTTCGCC
CATCTGTTGGACTAGCTGCACCACAAATTAACGAATTAAAAAGAATGTTTGCTATGCATGTAGAAGATATGGACGGTACC
CTTTATTCTTACGTGATGATTAACCCTGAAATTGTTGGTCATACTCACGAAGAAACCTACCTACCTGGTGGTGAAGGATG
TCTTTCAGTAGACCGTGCAACAAGTGGTTTAACACCAAGATACTACGGTATCATGGTAAGAGGCTATCATCTTGATTTTG
AAAGCTTAACGGCTAAACCAATTGAGTTAACCTTAGAAGGTTATCCTGCAATTGTATTCCAACACGAATATGATCATTTA
GACGGTATTATGTTTACATCTAAATTATATCCTAACTTACCAAATGCATTCCCGTTATTCGAAACATCTGAGGAAGAATC
AGAACCGATTGCTGAATAA

Upstream 100 bases:

>100_bases
TTTTCTACTCCCATAATTTAAGTTAGAGAGGAATTTGTCTAGTCTCATCATAATCTCCTTATGATATAATATGTCTTAGA
GGTGAATACATATGATTAAA

Downstream 100 bases:

>100_bases
ACTTAAGGCTTAGAACCCAAATTGATATGAGTTCTAAGCCTTTTATTTTTATTGATTATTTTAAATTAAACTGTAACACC
AACAGAGACAACTGGTTTAT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MNHIIREGHPSLTTPSKPVLLPLSQKDIDLGKSLIEYVKNSQDDAMVKKHRLRPSVGLAAPQINELKRMFAMHVEDMDGT
LYSYVMINPEIVGHTHEETYLPGGEGCLSVDRATSGLTPRYYGIMVRGYHLDFESLTAKPIELTLEGYPAIVFQHEYDHL
DGIMFTSKLYPNLPNAFPLFETSEEESEPIAE

Sequences:

>Translated_192_residues
MNHIIREGHPSLTTPSKPVLLPLSQKDIDLGKSLIEYVKNSQDDAMVKKHRLRPSVGLAAPQINELKRMFAMHVEDMDGT
LYSYVMINPEIVGHTHEETYLPGGEGCLSVDRATSGLTPRYYGIMVRGYHLDFESLTAKPIELTLEGYPAIVFQHEYDHL
DGIMFTSKLYPNLPNAFPLFETSEEESEPIAE
>Mature_192_residues
MNHIIREGHPSLTTPSKPVLLPLSQKDIDLGKSLIEYVKNSQDDAMVKKHRLRPSVGLAAPQINELKRMFAMHVEDMDGT
LYSYVMINPEIVGHTHEETYLPGGEGCLSVDRATSGLTPRYYGIMVRGYHLDFESLTAKPIELTLEGYPAIVFQHEYDHL
DGIMFTSKLYPNLPNAFPLFETSEEESEPIAE

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 21647; Mature: 21647

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHIIREGHPSLTTPSKPVLLPLSQKDIDLGKSLIEYVKNSQDDAMVKKHRLRPSVGLAA
CCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
PQINELKRMFAMHVEDMDGTLYSYVMINPEIVGHTHEETYLPGGEGCLSVDRATSGLTPR
CCHHHHHHHHHHHHHCCCCCEEEEEEECCEEECCCCCCCCCCCCCCEEEECCCCCCCCCC
YYGIMVRGYHLDFESLTAKPIELTLEGYPAIVFQHEYDHLDGIMFTSKLYPNLPNAFPLF
EEEEEEEEEEECHHHHCCCCEEEEECCCEEEEEEECCCCCCCEEEEHHHCCCCCCCCCCC
ETSEEESEPIAE
CCCCCCCCCCCC
>Mature Secondary Structure
MNHIIREGHPSLTTPSKPVLLPLSQKDIDLGKSLIEYVKNSQDDAMVKKHRLRPSVGLAA
CCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
PQINELKRMFAMHVEDMDGTLYSYVMINPEIVGHTHEETYLPGGEGCLSVDRATSGLTPR
CCHHHHHHHHHHHHHCCCCCEEEEEEECCEEECCCCCCCCCCCCCCEEEECCCCCCCCCC
YYGIMVRGYHLDFESLTAKPIELTLEGYPAIVFQHEYDHLDGIMFTSKLYPNLPNAFPLF
EEEEEEEEEEECHHHHCCCCEEEEECCCEEEEEEECCCCCCCEEEEHHHCCCCCCCCCCC
ETSEEESEPIAE
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9126850 [H]