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The map label for this gene is trpS [H]

Identifier: 162448025

GI number: 162448025

Start: 1235584

End: 1236588

Strand: Direct

Name: trpS [H]

Synonym: ACL_1182

Alternate gene names: 162448025

Gene position: 1235584-1236588 (Clockwise)

Preceding gene: 162448024

Following gene: 162448037

Centisome position: 82.54

GC content: 33.93

Gene sequence:

>1005_bases
ATGAAAAGACTTGTATCAGGCATTAAACCAACCGGTGAGTTAACACTTGGTAACTACATTGGTGCAATCAAACAATTTGT
TAAACTACAAAATGAATTACCAGATACCGAATTCTTTATTTTTATTGCTGACCTACATGCAATAACAACCCCACAAGATA
AGCAATTATTAAAAAAGAGAGTACGCGATATTGCAGCATACTATATCGCATGTGGACTGGATCCAAACAGAGTGAACTTA
TTTGTTCAATCTGAGGTGATGGAACACGCTATGCTTGGTTACATTATGGAATCTACTGCTTATATTAATGAAATGGAGCG
TATGATTCAGTTTAAAGAAAAGCGTACCTCACAAACTGAAGGCGTTCGAACTTCCCTTTTAACTTATCCAGCACTCATGG
CTGCAGATATTTTACTATATGATGCAAGTATCGTACCAGTTGGGGAAGACCAAACTCAGCACTTAGAACTTACAAGAACA
CTAGCTCAAAGATTTAATCACTTATATGGTGATACATTCGTCATACCTGAAGGTCATGTTACTAAAGTTGGTGCTAAGAT
TAAAGACCTTCAAGAACCAACCAAAAAAATGAGTAAGTCTGAGGGCTTATCTCAAAAATCTTATATTTTATTAGAAGATA
ATTTAAATCAAATTAAAAATAAGATCAAGTCTGCTGTAACAGATTCAGATACTAAGATATATTTTGATGAAGTCAATAAA
CCAGGTATCTCTAACTTGCTTACAATTTATTCAGCAATTACAGATACACCAATGAGTGATATCGTGGATAAATACAAAGA
TGAAACATCTTATGCCGGCTTTAAATCTGATTTAGCTGAAATCGTTGCAGAACATATTGGTCAAATCCAAGAAAATTATA
AAACTTTAATGGCTTCAAATACCTTAGACGATATATTAGATACGGGTGCTCAAAAAGCGTCCATGTATGCTCAAAGAAAA
ATTTTGAAAATCCTCTCGAAAATGGGCTTGAAAAGAAAAAAGTGA

Upstream 100 bases:

>100_bases
ATTTTTATCTAGAACTAAGGTGGTACCGCGAAATTACATTTCGTCCTTAGAAGTTTTATACTTTTAAGGACGATTTTTAT
TTATAGAAAGGAATTATATG

Downstream 100 bases:

>100_bases
TTTTATAATCACTTTTTTTTATACCATATTTTTATAAAACGCTTCAAGGTCTTCTCTTTCTAGAGGTTTAGATAGGTAAT
AGCCTTGTACAATGTCACAG

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKRLVSGIKPTGELTLGNYIGAIKQFVKLQNELPDTEFFIFIADLHAITTPQDKQLLKKRVRDIAAYYIACGLDPNRVNL
FVQSEVMEHAMLGYIMESTAYINEMERMIQFKEKRTSQTEGVRTSLLTYPALMAADILLYDASIVPVGEDQTQHLELTRT
LAQRFNHLYGDTFVIPEGHVTKVGAKIKDLQEPTKKMSKSEGLSQKSYILLEDNLNQIKNKIKSAVTDSDTKIYFDEVNK
PGISNLLTIYSAITDTPMSDIVDKYKDETSYAGFKSDLAEIVAEHIGQIQENYKTLMASNTLDDILDTGAQKASMYAQRK
ILKILSKMGLKRKK

Sequences:

>Translated_334_residues
MKRLVSGIKPTGELTLGNYIGAIKQFVKLQNELPDTEFFIFIADLHAITTPQDKQLLKKRVRDIAAYYIACGLDPNRVNL
FVQSEVMEHAMLGYIMESTAYINEMERMIQFKEKRTSQTEGVRTSLLTYPALMAADILLYDASIVPVGEDQTQHLELTRT
LAQRFNHLYGDTFVIPEGHVTKVGAKIKDLQEPTKKMSKSEGLSQKSYILLEDNLNQIKNKIKSAVTDSDTKIYFDEVNK
PGISNLLTIYSAITDTPMSDIVDKYKDETSYAGFKSDLAEIVAEHIGQIQENYKTLMASNTLDDILDTGAQKASMYAQRK
ILKILSKMGLKRKK
>Mature_334_residues
MKRLVSGIKPTGELTLGNYIGAIKQFVKLQNELPDTEFFIFIADLHAITTPQDKQLLKKRVRDIAAYYIACGLDPNRVNL
FVQSEVMEHAMLGYIMESTAYINEMERMIQFKEKRTSQTEGVRTSLLTYPALMAADILLYDASIVPVGEDQTQHLELTRT
LAQRFNHLYGDTFVIPEGHVTKVGAKIKDLQEPTKKMSKSEGLSQKSYILLEDNLNQIKNKIKSAVTDSDTKIYFDEVNK
PGISNLLTIYSAITDTPMSDIVDKYKDETSYAGFKSDLAEIVAEHIGQIQENYKTLMASNTLDDILDTGAQKASMYAQRK
ILKILSKMGLKRKK

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:
GO:0000166: Tryptophanyl-tRNA synthetase
GO:0004812: Tryptophanyl-tRNA synthetase
GO:0004830: Tryptophanyl-tRNA synthetase
GO:0005524: Tryptophanyl-tRNA synthetase
GO:0005737: Tryptophanyl-tRNA synthetase
GO:0006412: Tryptophanyl-tRNA synthetase
GO:0006418: Tryptophanyl-tRNA synthetase
GO:0006436: Tryptophanyl-tRNA synthetase
GO:0016874: Tryptophanyl-tRNA synthetase

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7710154, Length=317, Percent_Identity=38.4858044164038, Blast_Score=225, Evalue=4e-59,
Organism=Homo sapiens, GI41352700, Length=176, Percent_Identity=48.2954545454545, Blast_Score=174, Evalue=8e-44,
Organism=Escherichia coli, GI1789786, Length=333, Percent_Identity=43.8438438438438, Blast_Score=273, Evalue=9e-75,
Organism=Caenorhabditis elegans, GI71982800, Length=329, Percent_Identity=36.4741641337386, Blast_Score=198, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI71982793, Length=329, Percent_Identity=36.4741641337386, Blast_Score=197, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6320474, Length=344, Percent_Identity=35.1744186046512, Blast_Score=200, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24666151, Length=335, Percent_Identity=37.6119402985075, Blast_Score=220, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 37723; Mature: 37723

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLVSGIKPTGELTLGNYIGAIKQFVKLQNELPDTEFFIFIADLHAITTPQDKQLLKKR
CCHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHCCCHHHHHHHHH
VRDIAAYYIACGLDPNRVNLFVQSEVMEHAMLGYIMESTAYINEMERMIQFKEKRTSQTE
HHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVRTSLLTYPALMAADILLYDASIVPVGEDQTQHLELTRTLAQRFNHLYGDTFVIPEGHV
HHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCH
TKVGAKIKDLQEPTKKMSKSEGLSQKSYILLEDNLNQIKNKIKSAVTDSDTKIYFDEVNK
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCC
PGISNLLTIYSAITDTPMSDIVDKYKDETSYAGFKSDLAEIVAEHIGQIQENYKTLMASN
CCHHHHHHHHHHHHCCCHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TLDDILDTGAQKASMYAQRKILKILSKMGLKRKK
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKRLVSGIKPTGELTLGNYIGAIKQFVKLQNELPDTEFFIFIADLHAITTPQDKQLLKKR
CCHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHCCCHHHHHHHHH
VRDIAAYYIACGLDPNRVNLFVQSEVMEHAMLGYIMESTAYINEMERMIQFKEKRTSQTE
HHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVRTSLLTYPALMAADILLYDASIVPVGEDQTQHLELTRTLAQRFNHLYGDTFVIPEGHV
HHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCH
TKVGAKIKDLQEPTKKMSKSEGLSQKSYILLEDNLNQIKNKIKSAVTDSDTKIYFDEVNK
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCC
PGISNLLTIYSAITDTPMSDIVDKYKDETSYAGFKSDLAEIVAEHIGQIQENYKTLMASN
CCHHHHHHHHHHHHCCCHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TLDDILDTGAQKASMYAQRKILKILSKMGLKRKK
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]