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The map label for this gene is folC [H]

Identifier: 162447404

GI number: 162447404

Start: 578307

End: 579542

Strand: Direct

Name: folC [H]

Synonym: ACL_0542

Alternate gene names: 162447404

Gene position: 578307-579542 (Clockwise)

Preceding gene: 162447403

Following gene: 162447405

Centisome position: 38.63

GC content: 31.8

Gene sequence:

>1236_bases
ATGTTTAAACAACTTGATGAAGCAGTAAATTGGATAGAAACACAAATAAAGTTTAGACCTAAAACAAGCCTAGATCATAT
GAAGGATGCCTTAAGCATCCTTCAGCTTGATTTAAGCGGTATCAAAAAGATTCATGTCACTGGTACAAATGGTAAGGGCT
CAGTTTGTATGATGACTACTCAAGTACTGGTTGAATCCGGTATCCGCGTAGGAACTTTCATATCACCTTATTTGGTACGC
TTTAACGAACGCATTAAACTCAATGGTAAAGATATAGAAGATAATGATTTATTGGATTCTATTAATAAAGTTTATGAGCT
AAACAATGCATATTTTGAACATAGTGGGCTAACACTATCGTTTTTTGAACTGATGACTCTCATGTCACTGATTTACTTTG
TAGATAATAAGGTCGATGTTATCGTGATGGAAGTTGGTATTGGTGGTCTCTTAGATGCTACAAATATATTAAATTATGAT
GTCTCAGTCATTACCAATGTTGGAATGGATCATATGCGTCAATTAGGTAATACATTAGAATCGATTGCATTAAACAAACT
TGGCATTTTAAAAGAAAAGGGTCACTTAATTACGAGTATCGACAGTGGATTACATGATTTAGTTAAGGATTATGTATCAA
AAATGCATTCAACACTTAAAATTATTCAAAAACATGATGTAACCACTATATCCGAGTTTCCTCACAAAATTGAATATAAT
CACCAAATATATAAACTATCATTGTTAGGCGAGTTTCAAAACACTAATGCAGCGCTTGTTATAGAAGTTATTAAATATTT
ATTTCCAAATATAACTGAGCAAACAATTAGTGAAGGTTTAAGTAAAACTGAACACCCAGGACGCTTAGAAACAGTGTTGC
CTGGAGTGGTTTTAGATGGTGCCCATAATATTCATGCAATTAAGGAAGTCATTTTTTCGCTAAAATCTATTTTTAAAGTG
TATAATATACATGTACTATTCAGTGCACTATCTGATAAAGAGCCTGAAGTGATGCTAAATGAACTCAAAAAATATGCAAC
AACCATCACTTTGACTGCTTTTCCGGATGCTCGTTATAAAAACCTAGATTTTCTTCCCTTTAACTTTGAATTGAATCCTA
AAACAGCTTTAAAAAATTTAATCAAGAACAAAAAAGAAAATGACGTTATTTTTATAACTGGTAGTTTGCACTTTATTGGG
TATATGAAAAAGGAAATTATTCCGTACTTTAAATAA

Upstream 100 bases:

>100_bases
TTTACCTCAAAAGCGCCACAAGCTAAAATCGACATTGAACAAAGAAAGTATGACGACTATAAGAAACAATATGATGATGT
CGTTGAAATGTTAAAGAAAT

Downstream 100 bases:

>100_bases
GGTGATATATATGTTAATTAACCAGGTATTAATCTATTCAGAAAATCATAATGTTAATAACCCCAAAGGTACTGTCATTT
TTACACATGGCATTGCTTTA

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MFKQLDEAVNWIETQIKFRPKTSLDHMKDALSILQLDLSGIKKIHVTGTNGKGSVCMMTTQVLVESGIRVGTFISPYLVR
FNERIKLNGKDIEDNDLLDSINKVYELNNAYFEHSGLTLSFFELMTLMSLIYFVDNKVDVIVMEVGIGGLLDATNILNYD
VSVITNVGMDHMRQLGNTLESIALNKLGILKEKGHLITSIDSGLHDLVKDYVSKMHSTLKIIQKHDVTTISEFPHKIEYN
HQIYKLSLLGEFQNTNAALVIEVIKYLFPNITEQTISEGLSKTEHPGRLETVLPGVVLDGAHNIHAIKEVIFSLKSIFKV
YNIHVLFSALSDKEPEVMLNELKKYATTITLTAFPDARYKNLDFLPFNFELNPKTALKNLIKNKKENDVIFITGSLHFIG
YMKKEIIPYFK

Sequences:

>Translated_411_residues
MFKQLDEAVNWIETQIKFRPKTSLDHMKDALSILQLDLSGIKKIHVTGTNGKGSVCMMTTQVLVESGIRVGTFISPYLVR
FNERIKLNGKDIEDNDLLDSINKVYELNNAYFEHSGLTLSFFELMTLMSLIYFVDNKVDVIVMEVGIGGLLDATNILNYD
VSVITNVGMDHMRQLGNTLESIALNKLGILKEKGHLITSIDSGLHDLVKDYVSKMHSTLKIIQKHDVTTISEFPHKIEYN
HQIYKLSLLGEFQNTNAALVIEVIKYLFPNITEQTISEGLSKTEHPGRLETVLPGVVLDGAHNIHAIKEVIFSLKSIFKV
YNIHVLFSALSDKEPEVMLNELKKYATTITLTAFPDARYKNLDFLPFNFELNPKTALKNLIKNKKENDVIFITGSLHFIG
YMKKEIIPYFK
>Mature_411_residues
MFKQLDEAVNWIETQIKFRPKTSLDHMKDALSILQLDLSGIKKIHVTGTNGKGSVCMMTTQVLVESGIRVGTFISPYLVR
FNERIKLNGKDIEDNDLLDSINKVYELNNAYFEHSGLTLSFFELMTLMSLIYFVDNKVDVIVMEVGIGGLLDATNILNYD
VSVITNVGMDHMRQLGNTLESIALNKLGILKEKGHLITSIDSGLHDLVKDYVSKMHSTLKIIQKHDVTTISEFPHKIEYN
HQIYKLSLLGEFQNTNAALVIEVIKYLFPNITEQTISEGLSKTEHPGRLETVLPGVVLDGAHNIHAIKEVIFSLKSIFKV
YNIHVLFSALSDKEPEVMLNELKKYATTITLTAFPDARYKNLDFLPFNFELNPKTALKNLIKNKKENDVIFITGSLHFIG
YMKKEIIPYFK

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:
GO:0000166: Folylpolyglutamate synthase
GO:0004326: Folylpolyglutamate synthase
GO:0005524: Folylpolyglutamate synthase
GO:0009058: Folylpolyglutamate synthase
GO:0009396: Folylpolyglutamate synthase
GO:0016874: Folylpolyglutamate synthase
GO:0046901: Folylpolyglutamate synthase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=368, Percent_Identity=28.804347826087, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI66932990, Length=368, Percent_Identity=28.804347826087, Blast_Score=141, Evalue=1e-33,
Organism=Escherichia coli, GI1788654, Length=384, Percent_Identity=26.8229166666667, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17553150, Length=364, Percent_Identity=26.9230769230769, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71984923, Length=364, Percent_Identity=26.9230769230769, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17553148, Length=364, Percent_Identity=26.9230769230769, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323760, Length=385, Percent_Identity=28.8311688311688, Blast_Score=132, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6324815, Length=295, Percent_Identity=30.5084745762712, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24641571, Length=283, Percent_Identity=31.4487632508834, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24581568, Length=165, Percent_Identity=32.1212121212121, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 46615; Mature: 46615

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKQLDEAVNWIETQIKFRPKTSLDHMKDALSILQLDLSGIKKIHVTGTNGKGSVCMMTT
CCHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEHHH
QVLVESGIRVGTFISPYLVRFNERIKLNGKDIEDNDLLDSINKVYELNNAYFEHSGLTLS
HHHHHCCCCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCEEECCCCCHH
FFELMTLMSLIYFVDNKVDVIVMEVGIGGLLDATNILNYDVSVITNVGMDHMRQLGNTLE
HHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHH
SIALNKLGILKEKGHLITSIDSGLHDLVKDYVSKMHSTLKIIQKHDVTTISEFPHKIEYN
HHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCC
HQIYKLSLLGEFQNTNAALVIEVIKYLFPNITEQTISEGLSKTEHPGRLETVLPGVVLDG
CEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHCCCEEECC
AHNIHAIKEVIFSLKSIFKVYNIHVLFSALSDKEPEVMLNELKKYATTITLTAFPDARYK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEECCCCCCC
NLDFLPFNFELNPKTALKNLIKNKKENDVIFITGSLHFIGYMKKEIIPYFK
CCCEEEEEEECCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFKQLDEAVNWIETQIKFRPKTSLDHMKDALSILQLDLSGIKKIHVTGTNGKGSVCMMTT
CCHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEHHH
QVLVESGIRVGTFISPYLVRFNERIKLNGKDIEDNDLLDSINKVYELNNAYFEHSGLTLS
HHHHHCCCCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCEEECCCCCHH
FFELMTLMSLIYFVDNKVDVIVMEVGIGGLLDATNILNYDVSVITNVGMDHMRQLGNTLE
HHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHH
SIALNKLGILKEKGHLITSIDSGLHDLVKDYVSKMHSTLKIIQKHDVTTISEFPHKIEYN
HHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCC
HQIYKLSLLGEFQNTNAALVIEVIKYLFPNITEQTISEGLSKTEHPGRLETVLPGVVLDG
CEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHCCCEEECC
AHNIHAIKEVIFSLKSIFKVYNIHVLFSALSDKEPEVMLNELKKYATTITLTAFPDARYK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEECCCCCCC
NLDFLPFNFELNPKTALKNLIKNKKENDVIFITGSLHFIGYMKKEIIPYFK
CCCEEEEEEECCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]