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The map label for this gene is pldB [H]

Identifier: 162447405

GI number: 162447405

Start: 579553

End: 580332

Strand: Direct

Name: pldB [H]

Synonym: ACL_0543

Alternate gene names: 162447405

Gene position: 579553-580332 (Clockwise)

Preceding gene: 162447404

Following gene: 162447410

Centisome position: 38.71

GC content: 30.77

Gene sequence:

>780_bases
ATGTTAATTAACCAGGTATTAATCTATTCAGAAAATCATAATGTTAATAACCCCAAAGGTACTGTCATTTTTACACATGG
CATTGCTTTACACTCTATCTACTATAGAAAAATGGCTGAACTACTTAATGAAGCTGGTTTTTCTGTTGTTTTGTATGATG
TTAGAGGTCATGGTAAATCTCAAGGTAAAAGAGGAGATATTAAAAGTATTTACCAGTTTACTTCAGATTTATATGAACTT
ATAGAACAGACAAAAAAAGTCTATGATGCACCTATATACTTATTAGGCCACTCCATGGGTGGTATTATTACAAAAGTATA
TGCAACACTGTATGATAACTTTGAGGGAACTATTATTATGTCTAGTCCTACAAGTGCACAAAGACTAGGTCTTGTGAGTT
TACTCCCAAGTTTTTTATTTGGATCATTCAAAATTAATACAGATTTTACGGATACAAGATTATCTCACTTTCCTCCATCA
GATAATGTAGATCCGTATGCTTTAAAACATTTTACTTTTAGATTGATTATTCAAACCCTTAAAGTAGGTACTAAGCATAT
TGAAAAGCGAATTCAAGACTATAAGAAGCCTGTACTAATCTTACATGGTTCAGAAGATAAATTAGTAAGTCCTGAGATGT
CAAAACATTTCTTTGATAGTATTAAGCACAACGATAAAAAGTTAGCTATCATTGAAGGTGGTTATCATAATCTAAATTAT
GATACTGTGACTGAAAAAACGGTAGAAGAAATAGTTTCTTGGCTTAATCATCAAATTTAA

Upstream 100 bases:

>100_bases
AACAAAAAAGAAAATGACGTTATTTTTATAACTGGTAGTTTGCACTTTATTGGGTATATGAAAAAGGAAATTATTCCGTA
CTTTAAATAAGGTGATATAT

Downstream 100 bases:

>100_bases
TTACACTGTTCGTGAAAAAGGTTCCGCATAAATGCGGAACCTTTTTCATATATTAGAATTTATTGAATTTTGAATAAAAT
CTTTTTTTCTATAGTGGTTA

Product: lysophospholipase

Products: NA

Alternate protein names: Bacilysocin biosynthesis protein ytpA [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MLINQVLIYSENHNVNNPKGTVIFTHGIALHSIYYRKMAELLNEAGFSVVLYDVRGHGKSQGKRGDIKSIYQFTSDLYEL
IEQTKKVYDAPIYLLGHSMGGIITKVYATLYDNFEGTIIMSSPTSAQRLGLVSLLPSFLFGSFKINTDFTDTRLSHFPPS
DNVDPYALKHFTFRLIIQTLKVGTKHIEKRIQDYKKPVLILHGSEDKLVSPEMSKHFFDSIKHNDKKLAIIEGGYHNLNY
DTVTEKTVEEIVSWLNHQI

Sequences:

>Translated_259_residues
MLINQVLIYSENHNVNNPKGTVIFTHGIALHSIYYRKMAELLNEAGFSVVLYDVRGHGKSQGKRGDIKSIYQFTSDLYEL
IEQTKKVYDAPIYLLGHSMGGIITKVYATLYDNFEGTIIMSSPTSAQRLGLVSLLPSFLFGSFKINTDFTDTRLSHFPPS
DNVDPYALKHFTFRLIIQTLKVGTKHIEKRIQDYKKPVLILHGSEDKLVSPEMSKHFFDSIKHNDKKLAIIEGGYHNLNY
DTVTEKTVEEIVSWLNHQI
>Mature_259_residues
MLINQVLIYSENHNVNNPKGTVIFTHGIALHSIYYRKMAELLNEAGFSVVLYDVRGHGKSQGKRGDIKSIYQFTSDLYEL
IEQTKKVYDAPIYLLGHSMGGIITKVYATLYDNFEGTIIMSSPTSAQRLGLVSLLPSFLFGSFKINTDFTDTRLSHFPPS
DNVDPYALKHFTFRLIIQTLKVGTKHIEKRIQDYKKPVLILHGSEDKLVSPEMSKHFFDSIKHNDKKLAIIEGGYHNLNY
DTVTEKTVEEIVSWLNHQI

Specific function: Phospholipase involved in the biosynthesis of the antibiotic bacilysocin. It probably catalyzes the hydrolysis of the 2-sn-acyl moiety of phosphatidylglycerol to produce bacilysocin (lysophosphatidylglycerol). Is also able to catalyze the hydrolysis react

COG id: COG2267

COG function: function code I; Lysophospholipase

Gene ontology:
GO:0004622: Lysophospholipase
GO:0016787: Lysophospholipase

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily [H]

Homologues:

Organism=Homo sapiens, GI51242953, Length=262, Percent_Identity=27.4809160305344, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI6005786, Length=262, Percent_Identity=27.4809160305344, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR022742 [H]

Pfam domain/function: PF12146 Hydrolase_4 [H]

EC number: NA

Molecular weight: Translated: 29541; Mature: 29541

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLINQVLIYSENHNVNNPKGTVIFTHGIALHSIYYRKMAELLNEAGFSVVLYDVRGHGKS
CCCCEEEEEECCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
QGKRGDIKSIYQFTSDLYELIEQTKKVYDAPIYLLGHSMGGIITKVYATLYDNFEGTIIM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCCEEEE
SSPTSAQRLGLVSLLPSFLFGSFKINTDFTDTRLSHFPPSDNVDPYALKHFTFRLIIQTL
ECCCCHHHHHHHHHHHHHHHCCEEECCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHH
KVGTKHIEKRIQDYKKPVLILHGSEDKLVSPEMSKHFFDSIKHNDKKLAIIEGGYHNLNY
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCC
DTVTEKTVEEIVSWLNHQI
CHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLINQVLIYSENHNVNNPKGTVIFTHGIALHSIYYRKMAELLNEAGFSVVLYDVRGHGKS
CCCCEEEEEECCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
QGKRGDIKSIYQFTSDLYELIEQTKKVYDAPIYLLGHSMGGIITKVYATLYDNFEGTIIM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCCEEEE
SSPTSAQRLGLVSLLPSFLFGSFKINTDFTDTRLSHFPPSDNVDPYALKHFTFRLIIQTL
ECCCCHHHHHHHHHHHHHHHCCEEECCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHH
KVGTKHIEKRIQDYKKPVLILHGSEDKLVSPEMSKHFFDSIKHNDKKLAIIEGGYHNLNY
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCC
DTVTEKTVEEIVSWLNHQI
CHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]