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The map label for this gene is glgB [H]

Identifier: 162447391

GI number: 162447391

Start: 561317

End: 563179

Strand: Direct

Name: glgB [H]

Synonym: ACL_0529

Alternate gene names: 162447391

Gene position: 561317-563179 (Clockwise)

Preceding gene: 162447390

Following gene: 162447393

Centisome position: 37.5

GC content: 34.19

Gene sequence:

>1863_bases
TTGGGGAGGTACATAAAATTGGCATTTACAGAATCTCATGCAAATTTATATCTATTAGGCAAGAACTATTTAGCACATCA
GTTTTTAGGTGCACACCTGAATAAGGATGATGATGGCAACATCATATCTACTACATTTACTGTTTATGCACCCAACGCAA
AAGAAGTTAGACTTATTGCAGATTTCAACAATTACGAAGGCTGGAAGCATGTTCTAACTAAAATTCATCCACAAGGATTT
TGGTCCATTACAATACCCAAAGACCTTGAATGGGCAACTTATAAATATGAGATTCATCTAAAGAATGGAAAGATCTCACA
TAAATCAGATCCGTTTGGATACTTTTCTGAAGTAAGACCATCAAACGCATCTAAAGTTTATGATATTGATGGGTATCATT
GGAAGGATCATGACTGGTTTAAAAACAAGAAAAAAGTCTATGATCAACCAATGCTTATCTATGAAGTGCATCTTGGCTCT
TGGCAAAGAAATAATGGTCAATTTAGACCGTATAATGAAGTGGTTAAAGATTTAATTGACCATGTAAAATACAACGGATT
TACCCATATTGAACTCATGCCAGTCTATGAACATCCACTGGATGACTCATGGGGGTATCAAGGTACTGGTTTTTTTGCGG
CAACTTCTAGATTTGGGTCACCAAAAGATTTAATGTATTTAATCGATCAGTGTCATCAACATGGTATTGGTATCATCATG
GACTGGGTTTTAGGACATATTAATAAAGATGACTTTGGTTTATTTAATTTTGATGGCACTTATCTTTATGAATATGAGGA
TACCTTTAAAAGAGAAAACATAACATGGGGTACTGCGAATCTAGACTTCAATAAAGGTATTACTAAAAGTTTTATGATGT
CTGCCCTTACTTTTTGGATGGATTACTTCCATGTTGATGGATTTAGAATTGATGCTGTATCTCACCTGTTTTATTATCTA
GGTGATGAGTCCAATGGTATCAATGAAGAAGCTATTGCATTTTTAAAAGAAATTTCAGCACATGTATTTAGTAAGGATGA
AACCTTCATCCTATCTGCAGAAGACTCAACGGCATTTCCACTCGTTACCAAACCAACCTTTCTTGGTGGTATTGGGTTCA
ACTATAAATGGAATATGGGCTTTATGAATGATGTCTTATCTTACTTTAAACTTGATCCGATATACAGGAAACATAACCAT
GATAAACTAACTTTTGGCTTAGTTTACGCATTTAATGAACAATTTATTTTACCCTTTAGCCATGATGAGGTCGTACACCT
AAAAGGTTCCTTAGTCAGTAAAATGCCGGGAAGTTATGAACAAAAGTTTGCGCATTATAGAAACCTTATGACATTCTATA
TGACACACCCGGGTAAAAAGTTACTATTTATGGGTCAGGAATTTGCACAAATTGCTGAATGGGATTTTAAAAAGGAACTT
GACTGGAACTTATTAAAGTTTCCGATACATGACGCTTTTAATAGATACTTTAGGGATTTAGCACATGTGTATAAACACCA
TGATGCACTTTATCAAAAAGATCATGATCCGTCAGGATTTAAATGGTTAATCGTGGATCATAAAGACACATCTGTCTTTG
CTTATGCAAGATACTCGGATAACGAAGTACTTGTCATGGTTCATAATCTAACTCCAAACTATTATCAAAGCTATGAAGTT
GGTGTACCTTTTGAAGGAACATATGAGGAAATATTAAATTCCAACAAAGACATTTATAATGGAACAAACTTATACAACGG
CTTACCACTTAAAACCTACAAAGAACCAAGAAATGGTCAACCATATCATCTCAAGGTACAACTTTCAGGGTTAACGAGTA
TTATCTTTAAATATAAAAAATGA

Upstream 100 bases:

>100_bases
AATCAAAAAATAATCAACTTATAAAGAAAATGAGCCTTATTTTAATAATAAGGCCATTTTTGTAGCATATTTATTGTAAA
ATAGGATTAAAGGACAAACA

Downstream 100 bases:

>100_bases
GAATCCTGTGTTAGGATTCTCATCTTGTTGATTTACGTTCTACTAATTCAACATTCATGACTTCATGAAGGGTGGATAAC
TCATCACCACGAATTAATCT

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE [H]

Number of amino acids: Translated: 620; Mature: 619

Protein sequence:

>620_residues
MGRYIKLAFTESHANLYLLGKNYLAHQFLGAHLNKDDDGNIISTTFTVYAPNAKEVRLIADFNNYEGWKHVLTKIHPQGF
WSITIPKDLEWATYKYEIHLKNGKISHKSDPFGYFSEVRPSNASKVYDIDGYHWKDHDWFKNKKKVYDQPMLIYEVHLGS
WQRNNGQFRPYNEVVKDLIDHVKYNGFTHIELMPVYEHPLDDSWGYQGTGFFAATSRFGSPKDLMYLIDQCHQHGIGIIM
DWVLGHINKDDFGLFNFDGTYLYEYEDTFKRENITWGTANLDFNKGITKSFMMSALTFWMDYFHVDGFRIDAVSHLFYYL
GDESNGINEEAIAFLKEISAHVFSKDETFILSAEDSTAFPLVTKPTFLGGIGFNYKWNMGFMNDVLSYFKLDPIYRKHNH
DKLTFGLVYAFNEQFILPFSHDEVVHLKGSLVSKMPGSYEQKFAHYRNLMTFYMTHPGKKLLFMGQEFAQIAEWDFKKEL
DWNLLKFPIHDAFNRYFRDLAHVYKHHDALYQKDHDPSGFKWLIVDHKDTSVFAYARYSDNEVLVMVHNLTPNYYQSYEV
GVPFEGTYEEILNSNKDIYNGTNLYNGLPLKTYKEPRNGQPYHLKVQLSGLTSIIFKYKK

Sequences:

>Translated_620_residues
MGRYIKLAFTESHANLYLLGKNYLAHQFLGAHLNKDDDGNIISTTFTVYAPNAKEVRLIADFNNYEGWKHVLTKIHPQGF
WSITIPKDLEWATYKYEIHLKNGKISHKSDPFGYFSEVRPSNASKVYDIDGYHWKDHDWFKNKKKVYDQPMLIYEVHLGS
WQRNNGQFRPYNEVVKDLIDHVKYNGFTHIELMPVYEHPLDDSWGYQGTGFFAATSRFGSPKDLMYLIDQCHQHGIGIIM
DWVLGHINKDDFGLFNFDGTYLYEYEDTFKRENITWGTANLDFNKGITKSFMMSALTFWMDYFHVDGFRIDAVSHLFYYL
GDESNGINEEAIAFLKEISAHVFSKDETFILSAEDSTAFPLVTKPTFLGGIGFNYKWNMGFMNDVLSYFKLDPIYRKHNH
DKLTFGLVYAFNEQFILPFSHDEVVHLKGSLVSKMPGSYEQKFAHYRNLMTFYMTHPGKKLLFMGQEFAQIAEWDFKKEL
DWNLLKFPIHDAFNRYFRDLAHVYKHHDALYQKDHDPSGFKWLIVDHKDTSVFAYARYSDNEVLVMVHNLTPNYYQSYEV
GVPFEGTYEEILNSNKDIYNGTNLYNGLPLKTYKEPRNGQPYHLKVQLSGLTSIIFKYKK
>Mature_619_residues
GRYIKLAFTESHANLYLLGKNYLAHQFLGAHLNKDDDGNIISTTFTVYAPNAKEVRLIADFNNYEGWKHVLTKIHPQGFW
SITIPKDLEWATYKYEIHLKNGKISHKSDPFGYFSEVRPSNASKVYDIDGYHWKDHDWFKNKKKVYDQPMLIYEVHLGSW
QRNNGQFRPYNEVVKDLIDHVKYNGFTHIELMPVYEHPLDDSWGYQGTGFFAATSRFGSPKDLMYLIDQCHQHGIGIIMD
WVLGHINKDDFGLFNFDGTYLYEYEDTFKRENITWGTANLDFNKGITKSFMMSALTFWMDYFHVDGFRIDAVSHLFYYLG
DESNGINEEAIAFLKEISAHVFSKDETFILSAEDSTAFPLVTKPTFLGGIGFNYKWNMGFMNDVLSYFKLDPIYRKHNHD
KLTFGLVYAFNEQFILPFSHDEVVHLKGSLVSKMPGSYEQKFAHYRNLMTFYMTHPGKKLLFMGQEFAQIAEWDFKKELD
WNLLKFPIHDAFNRYFRDLAHVYKHHDALYQKDHDPSGFKWLIVDHKDTSVFAYARYSDNEVLVMVHNLTPNYYQSYEVG
VPFEGTYEEILNSNKDIYNGTNLYNGLPLKTYKEPRNGQPYHLKVQLSGLTSIIFKYKK

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position [H]

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=625, Percent_Identity=23.84, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI1789839, Length=620, Percent_Identity=39.3548387096774, Blast_Score=450, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17554896, Length=623, Percent_Identity=23.5955056179775, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI32564391, Length=598, Percent_Identity=22.9096989966555, Blast_Score=135, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6320826, Length=619, Percent_Identity=25.8481421647819, Blast_Score=144, Evalue=4e-35,
Organism=Drosophila melanogaster, GI28573410, Length=638, Percent_Identity=23.8244514106583, Blast_Score=165, Evalue=7e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48 [H]

EC number: =2.4.1.18 [H]

Molecular weight: Translated: 72751; Mature: 72620

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRYIKLAFTESHANLYLLGKNYLAHQFLGAHLNKDDDGNIISTTFTVYAPNAKEVRLIA
CCCEEEEEEEECCCEEEEEECCHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCEEEEEE
DFNNYEGWKHVLTKIHPQGFWSITIPKDLEWATYKYEIHLKNGKISHKSDPFGYFSEVRP
ECCCCCHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCCCHHHHCCC
SNASKVYDIDGYHWKDHDWFKNKKKVYDQPMLIYEVHLGSWQRNNGQFRPYNEVVKDLID
CCCCEEEECCCEEECCCHHHCCCHHHCCCCEEEEEEECCCEECCCCCCCCHHHHHHHHHH
HVKYNGFTHIELMPVYEHPLDDSWGYQGTGFFAATSRFGSPKDLMYLIDQCHQHGIGIIM
HHHCCCEEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEE
DWVLGHINKDDFGLFNFDGTYLYEYEDTFKRENITWGTANLDFNKGITKSFMMSALTFWM
HHHHHCCCCCCCEEEECCCCEEEEECHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
DYFHVDGFRIDAVSHLFYYLGDESNGINEEAIAFLKEISAHVFSKDETFILSAEDSTAFP
HHHHCCCEEEHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCE
LVTKPTFLGGIGFNYKWNMGFMNDVLSYFKLDPIYRKHNHDKLTFGLVYAFNEQFILPFS
EEECCCEEECCCCCEEECCCHHHHHHHHHHCCCHHHCCCCCEEEEEEEEEECCCEEEEEC
HDEVVHLKGSLVSKMPGSYEQKFAHYRNLMTFYMTHPGKKLLFMGQEFAQIAEWDFKKEL
CCCEEEECCHHHHHCCCCHHHHHHHHHHEEEEEEECCCCEEEEECHHHHHHHHCCCCCCC
DWNLLKFPIHDAFNRYFRDLAHVYKHHDALYQKDHDPSGFKWLIVDHKDTSVFAYARYSD
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEEEECC
NEVLVMVHNLTPNYYQSYEVGVPFEGTYEEILNSNKDIYNGTNLYNGLPLKTYKEPRNGQ
CEEEEEEEECCCCHHCCEECCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PYHLKVQLSGLTSIIFKYKK
CEEEEEEECCCEEEEEEECC
>Mature Secondary Structure 
GRYIKLAFTESHANLYLLGKNYLAHQFLGAHLNKDDDGNIISTTFTVYAPNAKEVRLIA
CCEEEEEEEECCCEEEEEECCHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCEEEEEE
DFNNYEGWKHVLTKIHPQGFWSITIPKDLEWATYKYEIHLKNGKISHKSDPFGYFSEVRP
ECCCCCHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCCCHHHHCCC
SNASKVYDIDGYHWKDHDWFKNKKKVYDQPMLIYEVHLGSWQRNNGQFRPYNEVVKDLID
CCCCEEEECCCEEECCCHHHCCCHHHCCCCEEEEEEECCCEECCCCCCCCHHHHHHHHHH
HVKYNGFTHIELMPVYEHPLDDSWGYQGTGFFAATSRFGSPKDLMYLIDQCHQHGIGIIM
HHHCCCEEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEE
DWVLGHINKDDFGLFNFDGTYLYEYEDTFKRENITWGTANLDFNKGITKSFMMSALTFWM
HHHHHCCCCCCCEEEECCCCEEEEECHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
DYFHVDGFRIDAVSHLFYYLGDESNGINEEAIAFLKEISAHVFSKDETFILSAEDSTAFP
HHHHCCCEEEHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCE
LVTKPTFLGGIGFNYKWNMGFMNDVLSYFKLDPIYRKHNHDKLTFGLVYAFNEQFILPFS
EEECCCEEECCCCCEEECCCHHHHHHHHHHCCCHHHCCCCCEEEEEEEEEECCCEEEEEC
HDEVVHLKGSLVSKMPGSYEQKFAHYRNLMTFYMTHPGKKLLFMGQEFAQIAEWDFKKEL
CCCEEEECCHHHHHCCCCHHHHHHHHHHEEEEEEECCCCEEEEECHHHHHHHHCCCCCCC
DWNLLKFPIHDAFNRYFRDLAHVYKHHDALYQKDHDPSGFKWLIVDHKDTSVFAYARYSD
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEEEECC
NEVLVMVHNLTPNYYQSYEVGVPFEGTYEEILNSNKDIYNGTNLYNGLPLKTYKEPRNGQ
CEEEEEEEECCCCHHCCEECCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PYHLKVQLSGLTSIIFKYKK
CEEEEEEECCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1296817 [H]