| Definition | Acholeplasma laidlawii PG-8A chromosome, complete genome. |
|---|---|
| Accession | NC_010163 |
| Length | 1,496,992 |
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The map label for this gene is amyX [H]
Identifier: 162447393
GI number: 162447393
Start: 564245
End: 566197
Strand: Direct
Name: amyX [H]
Synonym: ACL_0531
Alternate gene names: 162447393
Gene position: 564245-566197 (Clockwise)
Preceding gene: 162447391
Following gene: 162447394
Centisome position: 37.69
GC content: 29.75
Gene sequence:
>1953_bases ATGATTAAAGCTTTTATTGATGATTTACAATTAATTAGATTAGAATCTACAGATTATATTTATGATATTCATATCCAAGG ATATGAAATTACTTGGTATAAAAATGATGGTGACAATCAATATTTTAAGTCGAATATCGATTTAAAATTACATGAAGTCG ACCATATTTATATCAATCATCATAGATATCCATTACATATAGGATTAGTAACACTTCATAAGTCGTTTGATAAAAAATAT AGATACGATGGTCCACTTGGTGCAGTTTATACCAAGACACACACTGACTTTTATGTATATAGTCCGGTTGCTAAAGAAAT ATATTGTATGGTAGATGGTGCTACATATGAAATGACCTACTTAGAAGGTGGTGCATACCATGTTAGAGTTCAAGGAGATC TTGAAGGGAAAAGCTATGCATATAATGTAAGATTAGTGGATACATTCCAAATAGCAAATGATCCATACACTATAGCAAGT AACTTAGATTCATCTGTTATTATCGATATGGACAAATTAGAACCACAATTTCATGATCATGTCACATTAAAAAAATATAC AGATGCAGTCATTTATGAAGGTATGATAAGAGATTTAACCATTGATCTTGATGTACCAAATAAGGGTACTTTCTTAGGCG TAACAGAATACAGTGAAACTTTAAAATCATCTGTACTAGAGTATATCAAAAACTTAGGTGTTACACATTTTCAAGTGTTA CCAGTTTATGACTTTTATGGTGTAGATGATGTGAATAAAGATGAACTTTATAACTGGGGCTATAACCCGATGCAATACTT TGCAATAGAGGGTTGGCTATCAAATAATCCAAGTGATCCATATAGCAGAATAAACGAATTTAGAAAAGTAGTTGATAAAG CTCATGAAATTGGTTTAGCTGTAAATATGGACGTTGTTTACAATCATGTTTATGAACGTGCATTATTCCCTTATGATTAC TTTGTTCCAGGTTATTTTTATAGACACGATAAAAACTTTCAACAAACACACTCTGCATTTTTAGAAAATGACGTTGAAAC AACCAATTATATGGTTAGACGTTTAATTGTTGATTCATTAGTTCATTTTGTTAAACACTATAAAGTAGATGGATTTAGAT TTGATTTAATGGGATTAATGGATGTGGATACCATGAATCAAATAGCTATAGAACTTAAGAAGTTAAATCCAAACATCATG CTTTACGGTGAAGGTTGGAATATGGACAATGCACTACCTAAGTCAAAACGTAGTAACATGAACAACCAAGCAATGATGCC CGCTATTGGACACTTTAATGATTTTTATAGAAATTTATTAAAGGGTGAACTACATACAAAAGAACTTGGCTATGCTACTG GATCTAAAGAAAAATTAGAAGATGTATTAAAAGCATTAAAAGGTTCCCCTCATATATTTAATACACCAAATAAGTCATTA AACTATGTTGAATGTCATGACAATATGACACTTTTCGATACGTTACACTTTAACTATAAAGATAAGAAAATTAAGCATGT CTATCAAGATTTAGCAAATCACATCATCGCGATATCACAAGGTATACCTTTCTATCATGCTGGTCAGGAAATGTATCGTT CTAAAGATGGTGTTGAAAACTCTTATAAATCACCAGATAGTATTAATATGGTCAAATGGGAAAATTTTTCAAGTATTAAG AAATTAAGACAACTCTTAAAGATAAGAAAATCATACAAAGTATATCGTCATTCAGATTATAATGAAGATGTTCGTGCTGA AATTCATAGTGGTTACATACTTTATTCATTAAAATCTAATACGTATACACTAGATCATTACATTAAAAATGATTTTAATG AAATAAAAATACCATTAGATGGTAAACTAATATTTGGATCTAAGAATGTAAAAACACTAGATAATGAGATCTTAATCAAC AAACCAGGTGTTTATATTATCAAAAGGAAATAA
Upstream 100 bases:
>100_bases GCAACATCTTTAATGGTTGGTTTCATAGTTCACCTCGTATGTATTATACTTATTTTATCACAATAAACTAAAAAAAATGA AAGTGTAGGAAATATTACAA
Downstream 100 bases:
>100_bases CTCATATGAAAAAATATTTTATTGGAATTGACTGTGGTGGTACAAATATTAGAATCGGTTTAGTCGATGATGATTTTGTT ATTCATCATGAAGAAAAGCA
Product: alpha-amylase
Products: NA
Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]
Number of amino acids: Translated: 650; Mature: 650
Protein sequence:
>650_residues MIKAFIDDLQLIRLESTDYIYDIHIQGYEITWYKNDGDNQYFKSNIDLKLHEVDHIYINHHRYPLHIGLVTLHKSFDKKY RYDGPLGAVYTKTHTDFYVYSPVAKEIYCMVDGATYEMTYLEGGAYHVRVQGDLEGKSYAYNVRLVDTFQIANDPYTIAS NLDSSVIIDMDKLEPQFHDHVTLKKYTDAVIYEGMIRDLTIDLDVPNKGTFLGVTEYSETLKSSVLEYIKNLGVTHFQVL PVYDFYGVDDVNKDELYNWGYNPMQYFAIEGWLSNNPSDPYSRINEFRKVVDKAHEIGLAVNMDVVYNHVYERALFPYDY FVPGYFYRHDKNFQQTHSAFLENDVETTNYMVRRLIVDSLVHFVKHYKVDGFRFDLMGLMDVDTMNQIAIELKKLNPNIM LYGEGWNMDNALPKSKRSNMNNQAMMPAIGHFNDFYRNLLKGELHTKELGYATGSKEKLEDVLKALKGSPHIFNTPNKSL NYVECHDNMTLFDTLHFNYKDKKIKHVYQDLANHIIAISQGIPFYHAGQEMYRSKDGVENSYKSPDSINMVKWENFSSIK KLRQLLKIRKSYKVYRHSDYNEDVRAEIHSGYILYSLKSNTYTLDHYIKNDFNEIKIPLDGKLIFGSKNVKTLDNEILIN KPGVYIIKRK
Sequences:
>Translated_650_residues MIKAFIDDLQLIRLESTDYIYDIHIQGYEITWYKNDGDNQYFKSNIDLKLHEVDHIYINHHRYPLHIGLVTLHKSFDKKY RYDGPLGAVYTKTHTDFYVYSPVAKEIYCMVDGATYEMTYLEGGAYHVRVQGDLEGKSYAYNVRLVDTFQIANDPYTIAS NLDSSVIIDMDKLEPQFHDHVTLKKYTDAVIYEGMIRDLTIDLDVPNKGTFLGVTEYSETLKSSVLEYIKNLGVTHFQVL PVYDFYGVDDVNKDELYNWGYNPMQYFAIEGWLSNNPSDPYSRINEFRKVVDKAHEIGLAVNMDVVYNHVYERALFPYDY FVPGYFYRHDKNFQQTHSAFLENDVETTNYMVRRLIVDSLVHFVKHYKVDGFRFDLMGLMDVDTMNQIAIELKKLNPNIM LYGEGWNMDNALPKSKRSNMNNQAMMPAIGHFNDFYRNLLKGELHTKELGYATGSKEKLEDVLKALKGSPHIFNTPNKSL NYVECHDNMTLFDTLHFNYKDKKIKHVYQDLANHIIAISQGIPFYHAGQEMYRSKDGVENSYKSPDSINMVKWENFSSIK KLRQLLKIRKSYKVYRHSDYNEDVRAEIHSGYILYSLKSNTYTLDHYIKNDFNEIKIPLDGKLIFGSKNVKTLDNEILIN KPGVYIIKRK >Mature_650_residues MIKAFIDDLQLIRLESTDYIYDIHIQGYEITWYKNDGDNQYFKSNIDLKLHEVDHIYINHHRYPLHIGLVTLHKSFDKKY RYDGPLGAVYTKTHTDFYVYSPVAKEIYCMVDGATYEMTYLEGGAYHVRVQGDLEGKSYAYNVRLVDTFQIANDPYTIAS NLDSSVIIDMDKLEPQFHDHVTLKKYTDAVIYEGMIRDLTIDLDVPNKGTFLGVTEYSETLKSSVLEYIKNLGVTHFQVL PVYDFYGVDDVNKDELYNWGYNPMQYFAIEGWLSNNPSDPYSRINEFRKVVDKAHEIGLAVNMDVVYNHVYERALFPYDY FVPGYFYRHDKNFQQTHSAFLENDVETTNYMVRRLIVDSLVHFVKHYKVDGFRFDLMGLMDVDTMNQIAIELKKLNPNIM LYGEGWNMDNALPKSKRSNMNNQAMMPAIGHFNDFYRNLLKGELHTKELGYATGSKEKLEDVLKALKGSPHIFNTPNKSL NYVECHDNMTLFDTLHFNYKDKKIKHVYQDLANHIIAISQGIPFYHAGQEMYRSKDGVENSYKSPDSINMVKWENFSSIK KLRQLLKIRKSYKVYRHSDYNEDVRAEIHSGYILYSLKSNTYTLDHYIKNDFNEIKIPLDGKLIFGSKNVKTLDNEILIN KPGVYIIKRK
Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]
COG id: COG1523
COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glycosyl hydrolase 13 family [H]
Homologues:
Organism=Escherichia coli, GI2367229, Length=562, Percent_Identity=22.7758007117438, Blast_Score=100, Evalue=5e-22,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006047 - InterPro: IPR004193 - InterPro: IPR017853 - InterPro: IPR013781 - InterPro: IPR013783 - InterPro: IPR011840 [H]
Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]
EC number: =3.2.1.41 [H]
Molecular weight: Translated: 76071; Mature: 76071
Theoretical pI: Translated: 6.72; Mature: 6.72
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.3 %Cys (Translated Protein) 2.9 %Met (Translated Protein) 3.2 %Cys+Met (Translated Protein) 0.3 %Cys (Mature Protein) 2.9 %Met (Mature Protein) 3.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MIKAFIDDLQLIRLESTDYIYDIHIQGYEITWYKNDGDNQYFKSNIDLKLHEVDHIYINH CCCHHHCCEEEEEEECCCEEEEEEEEEEEEEEEECCCCCCEEECCCCEEEEEEEEEEEEC HRYPLHIGLVTLHKSFDKKYRYDGPLGAVYTKTHTDFYVYSPVAKEIYCMVDGATYEMTY CCCEEEEEEEEEEHHHCCCCCCCCCCCEEEEECCCCEEEECCCCCEEEEEECCCEEEEEE LEGGAYHVRVQGDLEGKSYAYNVRLVDTFQIANDPYTIASNLDSSVIIDMDKLEPQFHDH EECCEEEEEEEECCCCCEEEEEEEEEEEEEECCCCEEEECCCCCEEEEEHHHCCCCHHCC VTLKKYTDAVIYEGMIRDLTIDLDVPNKGTFLGVTEYSETLKSSVLEYIKNLGVTHFQVL EEHHHHHHHHHHCCCEEEEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEEE PVYDFYGVDDVNKDELYNWGYNPMQYFAIEGWLSNNPSDPYSRINEFRKVVDKAHEIGLA EEEEECCCCCCCHHHHCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEE VNMDVVYNHVYERALFPYDYFVPGYFYRHDKNFQQTHSAFLENDVETTNYMVRRLIVDSL EEHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH VHFVKHYKVDGFRFDLMGLMDVDTMNQIAIELKKLNPNIMLYGEGWNMDNALPKSKRSNM HHHHHHHCCCCEEEEEEEEECCCCHHHHEEEEEECCCCEEEEECCCCCCCCCCCHHHCCC NNQAMMPAIGHFNDFYRNLLKGELHTKELGYATGSKEKLEDVLKALKGSPHIFNTPNKSL CCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCEECCCCCCC NYVECHDNMTLFDTLHFNYKDKKIKHVYQDLANHIIAISQGIPFYHAGQEMYRSKDGVEN CEEEECCCEEEEEEEEECCCHHHHHHHHHHHHHHEEEEECCCCCEECCHHHHHCCCCCCC SYKSPDSINMVKWENFSSIKKLRQLLKIRKSYKVYRHSDYNEDVRAEIHSGYILYSLKSN CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHEECCCEEEEEECCC TYTLDHYIKNDFNEIKIPLDGKLIFGSKNVKTLDNEILINKPGVYIIKRK CEEEHHHHCCCCCEEEEEECCEEEECCCCCEECCCEEEEECCCEEEEEEC >Mature Secondary Structure MIKAFIDDLQLIRLESTDYIYDIHIQGYEITWYKNDGDNQYFKSNIDLKLHEVDHIYINH CCCHHHCCEEEEEEECCCEEEEEEEEEEEEEEEECCCCCCEEECCCCEEEEEEEEEEEEC HRYPLHIGLVTLHKSFDKKYRYDGPLGAVYTKTHTDFYVYSPVAKEIYCMVDGATYEMTY CCCEEEEEEEEEEHHHCCCCCCCCCCCEEEEECCCCEEEECCCCCEEEEEECCCEEEEEE LEGGAYHVRVQGDLEGKSYAYNVRLVDTFQIANDPYTIASNLDSSVIIDMDKLEPQFHDH EECCEEEEEEEECCCCCEEEEEEEEEEEEEECCCCEEEECCCCCEEEEEHHHCCCCHHCC VTLKKYTDAVIYEGMIRDLTIDLDVPNKGTFLGVTEYSETLKSSVLEYIKNLGVTHFQVL EEHHHHHHHHHHCCCEEEEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEEE PVYDFYGVDDVNKDELYNWGYNPMQYFAIEGWLSNNPSDPYSRINEFRKVVDKAHEIGLA EEEEECCCCCCCHHHHCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEE VNMDVVYNHVYERALFPYDYFVPGYFYRHDKNFQQTHSAFLENDVETTNYMVRRLIVDSL EEHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH VHFVKHYKVDGFRFDLMGLMDVDTMNQIAIELKKLNPNIMLYGEGWNMDNALPKSKRSNM HHHHHHHCCCCEEEEEEEEECCCCHHHHEEEEEECCCCEEEEECCCCCCCCCCCHHHCCC NNQAMMPAIGHFNDFYRNLLKGELHTKELGYATGSKEKLEDVLKALKGSPHIFNTPNKSL CCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCEECCCCCCC NYVECHDNMTLFDTLHFNYKDKKIKHVYQDLANHIIAISQGIPFYHAGQEMYRSKDGVEN CEEEECCCEEEEEEEEECCCHHHHHHHHHHHHHHEEEEECCCCCEECCHHHHHCCCCCCC SYKSPDSINMVKWENFSSIKKLRQLLKIRKSYKVYRHSDYNEDVRAEIHSGYILYSLKSN CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHEECCCEEEEEECCC TYTLDHYIKNDFNEIKIPLDGKLIFGSKNVKTLDNEILINKPGVYIIKRK CEEEHHHHCCCCCEEEEEECCEEEECCCCCEECCCEEEEECCCEEEEEEC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9387221; 9384377 [H]