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The map label for this gene is recN [H]

Identifier: 162447342

GI number: 162447342

Start: 501885

End: 503528

Strand: Direct

Name: recN [H]

Synonym: ACL_0479

Alternate gene names: 162447342

Gene position: 501885-503528 (Clockwise)

Preceding gene: 162447341

Following gene: 162447343

Centisome position: 33.53

GC content: 30.54

Gene sequence:

>1644_bases
ATGCTAAATTATCTAGAAGTTAAAAATTTCGCTTTAATTGATGATTTAAATATCGAGTTTAAGCATGGTTTAACATCATT
AACTGGAGAAACTGGTAGTGGTAAATCGATTCTTTTAGAGTCTTTACAATTGTTATTTGGAAAGCGTTCAGATGCTGAGT
ATATTAGAACTGGTACAACTAAAGCAACCGTTCTAGGTAGATTTAGTCTAACACCAAATCAAGCAAGAGATTTAGACTTA
CCCAATGATATTACACTGAGTAGAGAAATAGACCAATCAGGTAGACATCTAGTAAAACTAAATGATGAAACAATCACCCT
TGCTAGACTTAGACAAATAACGAATAAGATTGGCTTAATTCATGGACAAAATGATACCTATCAATTACTAGATAAATCTA
CCTATGTATCTTTTATTGACTTATTAAATCAAGTAGAAATCAACAAATTACTACAACATTATTTGCTCAAAAGAAGTACT
TATTTAGATGCTTTAAAGGCATTTGAAAACCTAAAAAATAAGAAACAAGAAACCCAAGAACGCGCTGAATTTTTAAAGTT
TCAAATCAACGAGTTAAAATCTTATAATCTAGTCTTAGGAGAAAAACAGGAATTAGAGGAAAAAGTTCAAAGATTAAAAA
ACTTTGATAGTATTCAGCAAGCACTAAAGCTAAGTTATGACACCTTTAATGGGCAATTTTTTCAAACAGATCTTTTATAT
GATGCATACAAACAAATGTCTAAAATCTCTGTATATGATAATGAATTTAAAGAAATATCAAAACTTCTTGAAGAAAGTTA
TTATAACTTAGAAGAAGCTAAAAAAAGTGTTGAATCTTCTTTAGATAGTCTAGATTTTGATAGTGATGCATACAATAATT
ATCAAGAACGTTTGTATGAACTATCTAAAATAGAGACGAAATATGCAAAATCAAATGATGACTTAGTGAAGTACTTAGAA
GAAATGACCGATGAGTTATTAATGAGTCAAGACTTTGATGGCTATTTAAAAATAGCTAAATCAAAACTGGATACACATTA
TGATGAAGCTTACAAACTTGGTGAAAAACTAAGTAGTTATAGAAAAAAACTAGCACTTAAGCTAGAAAAAGAATTAACTC
AGTCCTTACATGAACTTGATTTAGAAAAGGCAGTTTTTAAAATTGAGTTTGAAGCCTTAAAACCTCAGATGGTATTAGGT
GAGGATGGACTAGATTCAGTAGAGTTTATGATATCACTCAATGAAGGTGAACCCCTCAAAGCACTTGCTAAAGTAGCTAG
TGGTGGTGAAAAAGCTAGATTTATGTTTGCATTAAAATCTCTTTATGCCATAAATAGTGACTTAAGTCTCTTAGTTTTTG
ATGAGATTGACATTGGTATATCCGGAAAAACCGCAACAAAAATGGCAAATGTGATGAAACGCCATGCTAAAACGATGCAA
ATACTCGTGATAACACACTTACCACAAGTAGCTGCAAAGGCAGATTATCATTATGCAATCTATAAAGAATTAGTCGATAA
ACGTATGCAAACTCAAATTAATATCCTAAACTTTGAAGAACGTACTTTAGCGATCGCTAGTATGTTATCTGATGATGAAA
TTACACCGTTTGCGATTGAACAAGCTAAAAAATTATTATCATAA

Upstream 100 bases:

>100_bases
AAAGTCATTATCATTACAAATCTTAGGTTTTACACCAAGTCCAATAACCGGCAAAGATGGAAACCAAGAGTATTTAATTT
ACTGTACAAGGAGTTAATAC

Downstream 100 bases:

>100_bases
TAAAAAAATGAACTTATAAAGTAAGTTCATTTTATTTATTAAAAGGTATTGTTAATTAACTAAAAAAGTTAACGACTGCG
ATGATTGCACCAATTAAAAT

Product: DNA repair protein ATPase

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MLNYLEVKNFALIDDLNIEFKHGLTSLTGETGSGKSILLESLQLLFGKRSDAEYIRTGTTKATVLGRFSLTPNQARDLDL
PNDITLSREIDQSGRHLVKLNDETITLARLRQITNKIGLIHGQNDTYQLLDKSTYVSFIDLLNQVEINKLLQHYLLKRST
YLDALKAFENLKNKKQETQERAEFLKFQINELKSYNLVLGEKQELEEKVQRLKNFDSIQQALKLSYDTFNGQFFQTDLLY
DAYKQMSKISVYDNEFKEISKLLEESYYNLEEAKKSVESSLDSLDFDSDAYNNYQERLYELSKIETKYAKSNDDLVKYLE
EMTDELLMSQDFDGYLKIAKSKLDTHYDEAYKLGEKLSSYRKKLALKLEKELTQSLHELDLEKAVFKIEFEALKPQMVLG
EDGLDSVEFMISLNEGEPLKALAKVASGGEKARFMFALKSLYAINSDLSLLVFDEIDIGISGKTATKMANVMKRHAKTMQ
ILVITHLPQVAAKADYHYAIYKELVDKRMQTQINILNFEERTLAIASMLSDDEITPFAIEQAKKLLS

Sequences:

>Translated_547_residues
MLNYLEVKNFALIDDLNIEFKHGLTSLTGETGSGKSILLESLQLLFGKRSDAEYIRTGTTKATVLGRFSLTPNQARDLDL
PNDITLSREIDQSGRHLVKLNDETITLARLRQITNKIGLIHGQNDTYQLLDKSTYVSFIDLLNQVEINKLLQHYLLKRST
YLDALKAFENLKNKKQETQERAEFLKFQINELKSYNLVLGEKQELEEKVQRLKNFDSIQQALKLSYDTFNGQFFQTDLLY
DAYKQMSKISVYDNEFKEISKLLEESYYNLEEAKKSVESSLDSLDFDSDAYNNYQERLYELSKIETKYAKSNDDLVKYLE
EMTDELLMSQDFDGYLKIAKSKLDTHYDEAYKLGEKLSSYRKKLALKLEKELTQSLHELDLEKAVFKIEFEALKPQMVLG
EDGLDSVEFMISLNEGEPLKALAKVASGGEKARFMFALKSLYAINSDLSLLVFDEIDIGISGKTATKMANVMKRHAKTMQ
ILVITHLPQVAAKADYHYAIYKELVDKRMQTQINILNFEERTLAIASMLSDDEITPFAIEQAKKLLS
>Mature_547_residues
MLNYLEVKNFALIDDLNIEFKHGLTSLTGETGSGKSILLESLQLLFGKRSDAEYIRTGTTKATVLGRFSLTPNQARDLDL
PNDITLSREIDQSGRHLVKLNDETITLARLRQITNKIGLIHGQNDTYQLLDKSTYVSFIDLLNQVEINKLLQHYLLKRST
YLDALKAFENLKNKKQETQERAEFLKFQINELKSYNLVLGEKQELEEKVQRLKNFDSIQQALKLSYDTFNGQFFQTDLLY
DAYKQMSKISVYDNEFKEISKLLEESYYNLEEAKKSVESSLDSLDFDSDAYNNYQERLYELSKIETKYAKSNDDLVKYLE
EMTDELLMSQDFDGYLKIAKSKLDTHYDEAYKLGEKLSSYRKKLALKLEKELTQSLHELDLEKAVFKIEFEALKPQMVLG
EDGLDSVEFMISLNEGEPLKALAKVASGGEKARFMFALKSLYAINSDLSLLVFDEIDIGISGKTATKMANVMKRHAKTMQ
ILVITHLPQVAAKADYHYAIYKELVDKRMQTQINILNFEERTLAIASMLSDDEITPFAIEQAKKLLS

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=561, Percent_Identity=30.8377896613191, Blast_Score=266, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 62867; Mature: 62867

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNYLEVKNFALIDDLNIEFKHGLTSLTGETGSGKSILLESLQLLFGKRSDAEYIRTGTT
CCCCEEECCEEEEECCCCHHHCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCC
KATVLGRFSLTPNQARDLDLPNDITLSREIDQSGRHLVKLNDETITLARLRQITNKIGLI
CEEEEEEECCCCCCCCCCCCCCCCEEEHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCEE
HGQNDTYQLLDKSTYVSFIDLLNQVEINKLLQHYLLKRSTYLDALKAFENLKNKKQETQE
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAEFLKFQINELKSYNLVLGEKQELEEKVQRLKNFDSIQQALKLSYDTFNGQFFQTDLLY
HHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEHHHHHH
DAYKQMSKISVYDNEFKEISKLLEESYYNLEEAKKSVESSLDSLDFDSDAYNNYQERLYE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LSKIETKYAKSNDDLVKYLEEMTDELLMSQDFDGYLKIAKSKLDTHYDEAYKLGEKLSSY
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKKLALKLEKELTQSLHELDLEKAVFKIEFEALKPQMVLGEDGLDSVEFMISLNEGEPLK
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHCCCEEEECCCCCCCEEEEEEECCCCHHH
ALAKVASGGEKARFMFALKSLYAINSDLSLLVFDEIDIGISGKTATKMANVMKRHAKTMQ
HHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHE
ILVITHLPQVAAKADYHYAIYKELVDKRMQTQINILNFEERTLAIASMLSDDEITPFAIE
EEEEECCHHHHHHCCCHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHCCCCCCHHHHH
QAKKLLS
HHHHHCC
>Mature Secondary Structure
MLNYLEVKNFALIDDLNIEFKHGLTSLTGETGSGKSILLESLQLLFGKRSDAEYIRTGTT
CCCCEEECCEEEEECCCCHHHCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCC
KATVLGRFSLTPNQARDLDLPNDITLSREIDQSGRHLVKLNDETITLARLRQITNKIGLI
CEEEEEEECCCCCCCCCCCCCCCCEEEHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCEE
HGQNDTYQLLDKSTYVSFIDLLNQVEINKLLQHYLLKRSTYLDALKAFENLKNKKQETQE
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAEFLKFQINELKSYNLVLGEKQELEEKVQRLKNFDSIQQALKLSYDTFNGQFFQTDLLY
HHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEHHHHHH
DAYKQMSKISVYDNEFKEISKLLEESYYNLEEAKKSVESSLDSLDFDSDAYNNYQERLYE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LSKIETKYAKSNDDLVKYLEEMTDELLMSQDFDGYLKIAKSKLDTHYDEAYKLGEKLSSY
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKKLALKLEKELTQSLHELDLEKAVFKIEFEALKPQMVLGEDGLDSVEFMISLNEGEPLK
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHCCCEEEECCCCCCCEEEEEEECCCCHHH
ALAKVASGGEKARFMFALKSLYAINSDLSLLVFDEIDIGISGKTATKMANVMKRHAKTMQ
HHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHE
ILVITHLPQVAAKADYHYAIYKELVDKRMQTQINILNFEERTLAIASMLSDDEITPFAIE
EEEEECCHHHHHHCCCHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHCCCCCCHHHHH
QAKKLLS
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]