Click here to switch to the map view.

The map label for this gene is mucB [H]

Identifier: 162447343

GI number: 162447343

Start: 503790

End: 504941

Strand: Direct

Name: mucB [H]

Synonym: ACL_0480

Alternate gene names: 162447343

Gene position: 503790-504941 (Clockwise)

Preceding gene: 162447342

Following gene: 162447344

Centisome position: 33.65

GC content: 31.08

Gene sequence:

>1152_bases
ATGATATTATACTATATAATATGTATTAAAGGAAGTTTTAATTTGAAAAAATCATATAAAGTTATATTTCATATTGATTT
AAACGCTTTCTACGCTACATGTGCCATGATTAAAGAGCCACATTTGAAGCATAGGGTTTTTGTTGTTGGTGGACAATCCT
CTCAACCAAGAGGTGTTGTATCAACAGCAAGCTATAAAGCTAGAAAATATGGTATACATGCTGGCATGAGCATGTTAGAC
GCTTTAAATAAATTTCCCAGACTACTTATAGTACCTGTCGACTTTAAATTTTACAGAGAAATGTCTAACAAATTTATGGA
AGTTTTGGATGCTTATTCAGATTTAGTCCTTCAAGCATCCATTGATGAAGCGTTTTTAGATATGACCAAAGCATCTGAGG
AGATACACCCAATTAAGTTGGCTAAAAAGATTCAAAAGGAACTGGTTGATAAATACCAGTTACCAACATCTATTGGTATC
GCACCAACATTATTTTTAGCCAAAATGGCAAGTGATATGAAAAAACCATTAGGTATTACGGTGTTAAGAAAAAGAGATGT
AGAAAAGTTACTATATCCTTTAAGTGTTACTGACATTTATGGTATCGGTAAACAAACATACCCAAAACTCATGGCACTTG
GTATCAATACAATTAAAGACTTCATGGACTTATCCAACTTACCAAAGATTACACGTGTAATGAAAGAAGAAACTTATTAT
AGTTTTAGAGAATATATTTTAGGTAATAGTTCAAATGTTGTTGATCCGGATAAATACCGCTTACCAAAATCTATTTCTGC
TGAAACCACATTTAATACAAATATGGATGAACCGTCCGTTATATTAGACATCATCATGTCACAACTAGATGAATCCTTAG
TTAGACTAAAAAAATATAACATGCTTACAAAAACAGTTGGATTTAAACTCAAATCCAGTGATTTTAAATCCATTACCCGT
TCTAAAACACTAGAAGATTATACAAATGATCCTGAAATTATCAAATATGAACTGACATCACTTTTTGAAAATGAATTTGA
AGGTGGGATTTATCGTTTAGCTGGTGCATCATTAAGTAACTTAATTTTACACAGTGATAATGAAATTCCTTTTAATCTGT
TCACGTATGAAAAGTTTATCACTAAATTATGA

Upstream 100 bases:

>100_bases
GGATCTTTAAATACGTATGTATCTTTCTTTGCAACTGGTTGTTGTTTTTTAGAAACAGAATTCTTTTTTGCCATGTTATC
ACTCCAAAATAATTTGTCTT

Downstream 100 bases:

>100_bases
TATAATTATGGAGGTGAAAATATGAAAATAAAAGAAATGCTTGAGAAATTAAAGTATACTGAATTATCCCCAATACAAAC
TGAAGTCATTAAAAACTTTA

Product: DNA polymerase IV

Products: NA

Alternate protein names: Pol IV [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MILYYIICIKGSFNLKKSYKVIFHIDLNAFYATCAMIKEPHLKHRVFVVGGQSSQPRGVVSTASYKARKYGIHAGMSMLD
ALNKFPRLLIVPVDFKFYREMSNKFMEVLDAYSDLVLQASIDEAFLDMTKASEEIHPIKLAKKIQKELVDKYQLPTSIGI
APTLFLAKMASDMKKPLGITVLRKRDVEKLLYPLSVTDIYGIGKQTYPKLMALGINTIKDFMDLSNLPKITRVMKEETYY
SFREYILGNSSNVVDPDKYRLPKSISAETTFNTNMDEPSVILDIIMSQLDESLVRLKKYNMLTKTVGFKLKSSDFKSITR
SKTLEDYTNDPEIIKYELTSLFENEFEGGIYRLAGASLSNLILHSDNEIPFNLFTYEKFITKL

Sequences:

>Translated_383_residues
MILYYIICIKGSFNLKKSYKVIFHIDLNAFYATCAMIKEPHLKHRVFVVGGQSSQPRGVVSTASYKARKYGIHAGMSMLD
ALNKFPRLLIVPVDFKFYREMSNKFMEVLDAYSDLVLQASIDEAFLDMTKASEEIHPIKLAKKIQKELVDKYQLPTSIGI
APTLFLAKMASDMKKPLGITVLRKRDVEKLLYPLSVTDIYGIGKQTYPKLMALGINTIKDFMDLSNLPKITRVMKEETYY
SFREYILGNSSNVVDPDKYRLPKSISAETTFNTNMDEPSVILDIIMSQLDESLVRLKKYNMLTKTVGFKLKSSDFKSITR
SKTLEDYTNDPEIIKYELTSLFENEFEGGIYRLAGASLSNLILHSDNEIPFNLFTYEKFITKL
>Mature_383_residues
MILYYIICIKGSFNLKKSYKVIFHIDLNAFYATCAMIKEPHLKHRVFVVGGQSSQPRGVVSTASYKARKYGIHAGMSMLD
ALNKFPRLLIVPVDFKFYREMSNKFMEVLDAYSDLVLQASIDEAFLDMTKASEEIHPIKLAKKIQKELVDKYQLPTSIGI
APTLFLAKMASDMKKPLGITVLRKRDVEKLLYPLSVTDIYGIGKQTYPKLMALGINTIKDFMDLSNLPKITRVMKEETYY
SFREYILGNSSNVVDPDKYRLPKSISAETTFNTNMDEPSVILDIIMSQLDESLVRLKKYNMLTKTVGFKLKSSDFKSITR
SKTLEDYTNDPEIIKYELTSLFENEFEGGIYRLAGASLSNLILHSDNEIPFNLFTYEKFITKL

Specific function: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits

COG id: COG0389

COG function: function code L; Nucleotidyltransferase/DNA polymerase involved in DNA repair

Gene ontology:
GO:0000287: DNA polymerase IV
GO:0003677: DNA polymerase IV
GO:0003684: DNA polymerase IV
GO:0003887: DNA polymerase IV
GO:0005737: DNA polymerase IV
GO:0006260: DNA polymerase IV
GO:0006261: DNA polymerase IV
GO:0006281: DNA polymerase IV
GO:0006974: DNA polymerase IV
GO:0016740: DNA polymerase IV
GO:0016779: DNA polymerase IV
GO:0046872: DNA polymerase IV

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 umuC domain [H]

Homologues:

Organism=Homo sapiens, GI84043967, Length=341, Percent_Identity=26.3929618768328, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI7706681, Length=341, Percent_Identity=26.3929618768328, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI154350220, Length=250, Percent_Identity=31.6, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI7705344, Length=114, Percent_Identity=39.4736842105263, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1786425, Length=306, Percent_Identity=33.3333333333333, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1787432, Length=210, Percent_Identity=31.9047619047619, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17537959, Length=199, Percent_Identity=32.1608040201005, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI193205700, Length=408, Percent_Identity=28.921568627451, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI193205702, Length=347, Percent_Identity=28.5302593659942, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324921, Length=214, Percent_Identity=27.5700934579439, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19923006, Length=257, Percent_Identity=26.8482490272374, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017962
- InterPro:   IPR017961
- InterPro:   IPR001126
- InterPro:   IPR017963
- InterPro:   IPR022880 [H]

Pfam domain/function: PF00817 IMS [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 43834; Mature: 43834

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS50173 UMUC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILYYIICIKGSFNLKKSYKVIFHIDLNAFYATCAMIKEPHLKHRVFVVGGQSSQPRGVV
CEEEEEEEEECCCCCCCCEEEEEEEECHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEE
STASYKARKYGIHAGMSMLDALNKFPRLLIVPVDFKFYREMSNKFMEVLDAYSDLVLQAS
ECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
IDEAFLDMTKASEEIHPIKLAKKIQKELVDKYQLPTSIGIAPTLFLAKMASDMKKPLGIT
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEE
VLRKRDVEKLLYPLSVTDIYGIGKQTYPKLMALGINTIKDFMDLSNLPKITRVMKEETYY
EEHHHCHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
SFREYILGNSSNVVDPDKYRLPKSISAETTFNTNMDEPSVILDIIMSQLDESLVRLKKYN
HHHHHHCCCCCCCCCCHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MLTKTVGFKLKSSDFKSITRSKTLEDYTNDPEIIKYELTSLFENEFEGGIYRLAGASLSN
HHHHHHCCEECCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCEEEECCCCHHH
LILHSDNEIPFNLFTYEKFITKL
EEEECCCCCCEEHHHHHHHHHCC
>Mature Secondary Structure
MILYYIICIKGSFNLKKSYKVIFHIDLNAFYATCAMIKEPHLKHRVFVVGGQSSQPRGVV
CEEEEEEEEECCCCCCCCEEEEEEEECHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEE
STASYKARKYGIHAGMSMLDALNKFPRLLIVPVDFKFYREMSNKFMEVLDAYSDLVLQAS
ECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
IDEAFLDMTKASEEIHPIKLAKKIQKELVDKYQLPTSIGIAPTLFLAKMASDMKKPLGIT
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEE
VLRKRDVEKLLYPLSVTDIYGIGKQTYPKLMALGINTIKDFMDLSNLPKITRVMKEETYY
EEHHHCHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
SFREYILGNSSNVVDPDKYRLPKSISAETTFNTNMDEPSVILDIIMSQLDESLVRLKKYN
HHHHHHCCCCCCCCCCHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MLTKTVGFKLKSSDFKSITRSKTLEDYTNDPEIIKYELTSLFENEFEGGIYRLAGASLSN
HHHHHHCCEECCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCEEEECCCCHHH
LILHSDNEIPFNLFTYEKFITKL
EEEECCCCCCEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721630 [H]