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The map label for this gene is potD-A [H]

Identifier: 162447337

GI number: 162447337

Start: 498069

End: 499136

Strand: Direct

Name: potD-A [H]

Synonym: ACL_0474

Alternate gene names: 162447337

Gene position: 498069-499136 (Clockwise)

Preceding gene: 162447336

Following gene: 162447338

Centisome position: 33.27

GC content: 30.43

Gene sequence:

>1068_bases
ATGAAGAAAATATTTAGTTTAACATTTATTATGGCAGCATTATTTATATTAAATGCTTGTACATCCAATGCATTAGTAAT
TTATATGCCAAAAGAATATATCTCAGAAGATCTAATTGATGCTTTTGAACAAGAAACAGGCATTCAAGTCGCACTACGTT
ACTTTGATTCAAACGAAGTACTACTAACAAATGCTAAAGTAAACTCATACGATTTAATTATTCCATCAGACTATGCAATT
GAAGAATTAGCAGCTGGGGATTATTTAAAACCACTTGATTGGGACCGTATTGACTTTGATAAAGCTGATTTTTCAGTGAA
TTTATCAAATGCAATTGACCAATTAAATACCGGTGGATTTGATATTTTAGAATATTCACTACCATATTTTTGGGGTACAG
TTGGATTAATCTATAACAATACTAAAGCTGGCTTATATGAAGATCTTGTTGAAGAAGAATGGAATATCCTAAATAATCCA
GACTATTCAAAAATGATTTATGATTCATCAAGAGATGCATTTATGGCGGCTCTATATGCTAATAATTTTGAAATGAAAAA
TGCAACATCAACTGAAATAAATGTTGCTAAAAACTGGTTAATTGATGCATACTCAAAATCAAATGCATCTATAAAGTCAG
ATGAAATTTTAAGTGAAGCGATTGATAATAACACACCATATGATGTAGCAATGGTTTACTCAGGTGATGCGGTATACATC
TTACAAGAAAATGAAAAATATTCATACTTTGTACCAACTTGGACAAACGTATGGATTGATGGCTATGTTATTCCAAAAAA
TACTAAACATGAAGATTGGGCATATGAATTTATCAACTTCATGTCCACTTATGATGCATTACTTGATAATGCAACAGAAA
TGGGTTATTCACCAATTTCTGAAGCAGTTTACCAAAACTTATATCAAGACGAAGAATTTAACTGGGACAATGACCGTATT
GGTTATGCATTCTCAGTACCTTATACAAATTTTGAATTCTTTAGATATGATGTAGATTTAAAGAAATTAATGGATGATGC
ATGGGAACTTGTCATCTTAAGTAACTAA

Upstream 100 bases:

>100_bases
ATTAACGCATTATCCACCATCATTATCTTTATTATTGGCATCAAAGTCATTCTAGACTATGTCAATTTAAGAAATAAAAA
ACACGAGGAAGAGTAAAAAA

Downstream 100 bases:

>100_bases
ACCTAAATATATAAACCTAATAGTAATCGATAAGATAAGTACTATTAGGTTTTTTTATTAAACATAAATCATCGTGTATT
ATGTCACATAAGCGCATATT

Product: ABC transporter ligand-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKKIFSLTFIMAALFILNACTSNALVIYMPKEYISEDLIDAFEQETGIQVALRYFDSNEVLLTNAKVNSYDLIIPSDYAI
EELAAGDYLKPLDWDRIDFDKADFSVNLSNAIDQLNTGGFDILEYSLPYFWGTVGLIYNNTKAGLYEDLVEEEWNILNNP
DYSKMIYDSSRDAFMAALYANNFEMKNATSTEINVAKNWLIDAYSKSNASIKSDEILSEAIDNNTPYDVAMVYSGDAVYI
LQENEKYSYFVPTWTNVWIDGYVIPKNTKHEDWAYEFINFMSTYDALLDNATEMGYSPISEAVYQNLYQDEEFNWDNDRI
GYAFSVPYTNFEFFRYDVDLKKLMDDAWELVILSN

Sequences:

>Translated_355_residues
MKKIFSLTFIMAALFILNACTSNALVIYMPKEYISEDLIDAFEQETGIQVALRYFDSNEVLLTNAKVNSYDLIIPSDYAI
EELAAGDYLKPLDWDRIDFDKADFSVNLSNAIDQLNTGGFDILEYSLPYFWGTVGLIYNNTKAGLYEDLVEEEWNILNNP
DYSKMIYDSSRDAFMAALYANNFEMKNATSTEINVAKNWLIDAYSKSNASIKSDEILSEAIDNNTPYDVAMVYSGDAVYI
LQENEKYSYFVPTWTNVWIDGYVIPKNTKHEDWAYEFINFMSTYDALLDNATEMGYSPISEAVYQNLYQDEEFNWDNDRI
GYAFSVPYTNFEFFRYDVDLKKLMDDAWELVILSN
>Mature_355_residues
MKKIFSLTFIMAALFILNACTSNALVIYMPKEYISEDLIDAFEQETGIQVALRYFDSNEVLLTNAKVNSYDLIIPSDYAI
EELAAGDYLKPLDWDRIDFDKADFSVNLSNAIDQLNTGGFDILEYSLPYFWGTVGLIYNNTKAGLYEDLVEEEWNILNNP
DYSKMIYDSSRDAFMAALYANNFEMKNATSTEINVAKNWLIDAYSKSNASIKSDEILSEAIDNNTPYDVAMVYSGDAVYI
LQENEKYSYFVPTWTNVWIDGYVIPKNTKHEDWAYEFINFMSTYDALLDNATEMGYSPISEAVYQNLYQDEEFNWDNDRI
GYAFSVPYTNFEFFRYDVDLKKLMDDAWELVILSN

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=334, Percent_Identity=26.0479041916168, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787078, Length=300, Percent_Identity=25.6666666666667, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 41019; Mature: 41019

Theoretical pI: Translated: 3.80; Mature: 3.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIFSLTFIMAALFILNACTSNALVIYMPKEYISEDLIDAFEQETGIQVALRYFDSNEV
CCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHCCCEEEEEEEECCCCE
LLTNAKVNSYDLIIPSDYAIEELAAGDYLKPLDWDRIDFDKADFSVNLSNAIDQLNTGGF
EEEECCCCEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCE
DILEYSLPYFWGTVGLIYNNTKAGLYEDLVEEEWNILNNPDYSKMIYDSSRDAFMAALYA
EEEECCCCHHHCEEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHEEECCCCCCEEEEEEC
NNFEMKNATSTEINVAKNWLIDAYSKSNASIKSDEILSEAIDNNTPYDVAMVYSGDAVYI
CCCCCCCCCCCEEEEHHHHEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCEEEE
LQENEKYSYFVPTWTNVWIDGYVIPKNTKHEDWAYEFINFMSTYDALLDNATEMGYSPIS
EEECCCEEEECCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCHHH
EAVYQNLYQDEEFNWDNDRIGYAFSVPYTNFEFFRYDVDLKKLMDDAWELVILSN
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEEECCCHHHHHCCCEEEEEEEC
>Mature Secondary Structure
MKKIFSLTFIMAALFILNACTSNALVIYMPKEYISEDLIDAFEQETGIQVALRYFDSNEV
CCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHCCCEEEEEEEECCCCE
LLTNAKVNSYDLIIPSDYAIEELAAGDYLKPLDWDRIDFDKADFSVNLSNAIDQLNTGGF
EEEECCCCEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCE
DILEYSLPYFWGTVGLIYNNTKAGLYEDLVEEEWNILNNPDYSKMIYDSSRDAFMAALYA
EEEECCCCHHHCEEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHEEECCCCCCEEEEEEC
NNFEMKNATSTEINVAKNWLIDAYSKSNASIKSDEILSEAIDNNTPYDVAMVYSGDAVYI
CCCCCCCCCCCEEEEHHHHEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCEEEE
LQENEKYSYFVPTWTNVWIDGYVIPKNTKHEDWAYEFINFMSTYDALLDNATEMGYSPIS
EEECCCEEEECCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCHHH
EAVYQNLYQDEEFNWDNDRIGYAFSVPYTNFEFFRYDVDLKKLMDDAWELVILSN
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEEECCCHHHHHCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]