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The map label for this gene is queA [H]

Identifier: 162447237

GI number: 162447237

Start: 390741

End: 391589

Strand: Direct

Name: queA [H]

Synonym: ACL_0371

Alternate gene names: 162447237

Gene position: 390741-391589 (Clockwise)

Preceding gene: 162447236

Following gene: 162447239

Centisome position: 26.1

GC content: 33.22

Gene sequence:

>849_bases
ATGAATAACACAAAAGTTATCCCTGCAAGATTAATTGGTGAAAAGATCGATACGCATGCAGTCATTGAAGTTTTACTACT
TAAAGAGTTAGAAAAAAACACTTGGGAAGCTTTAACGAAACCTGCCAAAAAAATTAAAATTGGTACTGTAGTAAGTTTTG
GTGATGGTGCACTTAAAATGGAGTGTATTGGCATTAAAGATGAAGGTATTCGCATCTTTAAAGCAATCTATGAAGGTATA
TTTTTAGAAGTACTAGATGCTTTAGGTACCATGCCACTACCACCTTACATAACGGAAAAATTAGAAGATAAATCAAGATA
TCAAACTGTTTATGCAAAATATGCAGGATCTGCTGCAGCACCTACTGCAGGACTCCATTTTACCAAAGAACTTTTAGAAA
AGATTAAAGCCAAAGGTGTTCAAGTATTAGAAATTACACTCCATGTTGGTTTAGGTACATTTAGACCAGTTCAAGTAGAT
GATGTATTAGAACATCAAATGCACTATGAAAGTTATGAGATAAACGAAGAGACTGCCCGATTATTAAACCAATATAAAAA
AGAAGGTAAAAGAATTATCGCAGTTGGTACAACCACTGTAAGAACACTAGAATCTAACTATAATGATGGATTCCATGCAG
GTAATTACTCAACCAACATCTTTATTTATCCGGGATATCAATTTAAGATCATTGGTGCGATTATCACTAATTTCCATTTA
CCAAAATCAACGCTCATCATGTTGATTTCAGCATTCTATAATAGAGAAAAAGTGCTTGAAGTCTATGAAGACGCAGTGAA
AAAAAACTACCGCTTTTTCTCATTTGGCGATAGTATGTTTATTAAATAA

Upstream 100 bases:

>100_bases
CCACTCAAAATTATTGGTTGTTGATAGAGAAAAACAAACAACAAAAGATTGTTTGTTTTATGACATCTTAGATGAGTTAA
GTAGCAATGATGTACTAGTT

Downstream 100 bases:

>100_bases
TATATAAAGTAATTGAAACTAGACCAACTGTTAAAGCATATAGAGAAAACCAAATTAAATTTTTAACAGTGACATATTTG
TGCATGATCTTTATAGTTAA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MNNTKVIPARLIGEKIDTHAVIEVLLLKELEKNTWEALTKPAKKIKIGTVVSFGDGALKMECIGIKDEGIRIFKAIYEGI
FLEVLDALGTMPLPPYITEKLEDKSRYQTVYAKYAGSAAAPTAGLHFTKELLEKIKAKGVQVLEITLHVGLGTFRPVQVD
DVLEHQMHYESYEINEETARLLNQYKKEGKRIIAVGTTTVRTLESNYNDGFHAGNYSTNIFIYPGYQFKIIGAIITNFHL
PKSTLIMLISAFYNREKVLEVYEDAVKKNYRFFSFGDSMFIK

Sequences:

>Translated_282_residues
MNNTKVIPARLIGEKIDTHAVIEVLLLKELEKNTWEALTKPAKKIKIGTVVSFGDGALKMECIGIKDEGIRIFKAIYEGI
FLEVLDALGTMPLPPYITEKLEDKSRYQTVYAKYAGSAAAPTAGLHFTKELLEKIKAKGVQVLEITLHVGLGTFRPVQVD
DVLEHQMHYESYEINEETARLLNQYKKEGKRIIAVGTTTVRTLESNYNDGFHAGNYSTNIFIYPGYQFKIIGAIITNFHL
PKSTLIMLISAFYNREKVLEVYEDAVKKNYRFFSFGDSMFIK
>Mature_282_residues
MNNTKVIPARLIGEKIDTHAVIEVLLLKELEKNTWEALTKPAKKIKIGTVVSFGDGALKMECIGIKDEGIRIFKAIYEGI
FLEVLDALGTMPLPPYITEKLEDKSRYQTVYAKYAGSAAAPTAGLHFTKELLEKIKAKGVQVLEITLHVGLGTFRPVQVD
DVLEHQMHYESYEINEETARLLNQYKKEGKRIIAVGTTTVRTLESNYNDGFHAGNYSTNIFIYPGYQFKIIGAIITNFHL
PKSTLIMLISAFYNREKVLEVYEDAVKKNYRFFSFGDSMFIK

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=286, Percent_Identity=45.4545454545455, Blast_Score=240, Evalue=7e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 31928; Mature: 31928

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNTKVIPARLIGEKIDTHAVIEVLLLKELEKNTWEALTKPAKKIKIGTVVSFGDGALKM
CCCCEEEHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHEEEEEEEEECCCCEEE
ECIGIKDEGIRIFKAIYEGIFLEVLDALGTMPLPPYITEKLEDKSRYQTVYAKYAGSAAA
EEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
PTAGLHFTKELLEKIKAKGVQVLEITLHVGLGTFRPVQVDDVLEHQMHYESYEINEETAR
CCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCHHHHH
LLNQYKKEGKRIIAVGTTTVRTLESNYNDGFHAGNYSTNIFIYPGYQFKIIGAIITNFHL
HHHHHHHCCCEEEEEECHHHEEHHCCCCCCCCCCCCCCEEEEECCCEEEEHHHHHHCCCC
PKSTLIMLISAFYNREKVLEVYEDAVKKNYRFFSFGDSMFIK
CHHHHHHHHHHHHCHHHHHHHHHHHHHCCCEEEEECCCEECC
>Mature Secondary Structure
MNNTKVIPARLIGEKIDTHAVIEVLLLKELEKNTWEALTKPAKKIKIGTVVSFGDGALKM
CCCCEEEHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHEEEEEEEEECCCCEEE
ECIGIKDEGIRIFKAIYEGIFLEVLDALGTMPLPPYITEKLEDKSRYQTVYAKYAGSAAA
EEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
PTAGLHFTKELLEKIKAKGVQVLEITLHVGLGTFRPVQVDDVLEHQMHYESYEINEETAR
CCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCHHHHH
LLNQYKKEGKRIIAVGTTTVRTLESNYNDGFHAGNYSTNIFIYPGYQFKIIGAIITNFHL
HHHHHHHCCCEEEEEECHHHEEHHCCCCCCCCCCCCCCEEEEECCCEEEEHHHHHHCCCC
PKSTLIMLISAFYNREKVLEVYEDAVKKNYRFFSFGDSMFIK
CHHHHHHHHHHHHCHHHHHHHHHHHHHCCCEEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA