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The map label for this gene is ruvB

Identifier: 162447236

GI number: 162447236

Start: 389560

End: 390573

Strand: Direct

Name: ruvB

Synonym: ACL_0370

Alternate gene names: 162447236

Gene position: 389560-390573 (Clockwise)

Preceding gene: 162447235

Following gene: 162447237

Centisome position: 26.02

GC content: 34.02

Gene sequence:

>1014_bases
ATGAGTGAAGAAAAATCAGTATTAAGAGATTTAAATTTAAAAAATGATGATGAATTGATGCTTCGTCCTCAAACCTTAAA
CCAATATATAGGGCAAGATGATATTAAAGAAATGTTATCTATTTATATTCAGGCAGCATTAAAACGTGAAGAAAGTTTAG
ATCACGTTTTACTTTATGGTGCACCGGGTTTAGGTAAGACAACACTTGCTCAAATTATCGCTAATGAGTTAGGTGTAGAT
ATCAAAATAACAAGTGGTCCAGCTATAGAAAAAACAGGTGACTTAGTAGCCCTTCTTTCTTCATTAAGTCCAGGAGATGT
GTTATTTATTGATGAAATTCACCGTATACCTCGTTTTGTTGAAGAGGTTCTTTATTCTGCAATGGAAGATTATACGCTTG
ATATTGTTCTAGATAAGGAAAGAGATTCTAGAAGTATTAGAATCGAATTACCACCGTTTACACTCATTGGTGCAACCACT
AGATTTGGTGATTTATCACACCCGTTAAGGGAACGATTTGGTGCTGTATTTAGATTAAGTTATTACAAGTTAGAAGAAAT
CAAACAAATTGTAAGAAGAACTTCTAAAGTATATCAAAATGAAATTGATGAAAAAGCAGTAGATGAATTATCTAAGCGTA
GTAGAGGTACACCTAGAATAGCAAACCGTCTATTTAGACGCGTAAGAGACTTTGCTGAAATTATGACAGATGCCGTGATA
ACACTAGATATTACACAGTTAGCATTGACCAAATTAGGCATTGATCATAAGGGTCTGGATGCATCAGACTATTTATATTT
AAGAGGCATTGTAGAGCGATTTAATGGTGGTCCTGTTGGACTTGAATCACTGGCTTCTACAATTGGTGAAGAACCAGGAA
CAATTGAAGATGTATATGAACCTTACTTGTTACAGGAAGGTTATATTAAACGTACACCAAGAGGTAGAGTTGCAACAGAA
CTTGCATACAACCTTTTAGGTGTGAAATATTATAAAGGATTATTAGACAATTGA

Upstream 100 bases:

>100_bases
AAAAGAGATTGCAAAATCATTAAAGCATGTTAATTTTGATCAAGACTTAAATAAAGCACTAAAAGAAGCACTTGCAATAT
TATTAAAGTAGGTATGACAC

Downstream 100 bases:

>100_bases
AATTAGAATTATTTGATTATGATTTACCCAAATCACATATAGCGCAACATCCAAAAGATAAACGAGACCACTCAAAATTA
TTGGTTGTTGATAGAGAAAA

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSEEKSVLRDLNLKNDDELMLRPQTLNQYIGQDDIKEMLSIYIQAALKREESLDHVLLYGAPGLGKTTLAQIIANELGVD
IKITSGPAIEKTGDLVALLSSLSPGDVLFIDEIHRIPRFVEEVLYSAMEDYTLDIVLDKERDSRSIRIELPPFTLIGATT
RFGDLSHPLRERFGAVFRLSYYKLEEIKQIVRRTSKVYQNEIDEKAVDELSKRSRGTPRIANRLFRRVRDFAEIMTDAVI
TLDITQLALTKLGIDHKGLDASDYLYLRGIVERFNGGPVGLESLASTIGEEPGTIEDVYEPYLLQEGYIKRTPRGRVATE
LAYNLLGVKYYKGLLDN

Sequences:

>Translated_337_residues
MSEEKSVLRDLNLKNDDELMLRPQTLNQYIGQDDIKEMLSIYIQAALKREESLDHVLLYGAPGLGKTTLAQIIANELGVD
IKITSGPAIEKTGDLVALLSSLSPGDVLFIDEIHRIPRFVEEVLYSAMEDYTLDIVLDKERDSRSIRIELPPFTLIGATT
RFGDLSHPLRERFGAVFRLSYYKLEEIKQIVRRTSKVYQNEIDEKAVDELSKRSRGTPRIANRLFRRVRDFAEIMTDAVI
TLDITQLALTKLGIDHKGLDASDYLYLRGIVERFNGGPVGLESLASTIGEEPGTIEDVYEPYLLQEGYIKRTPRGRVATE
LAYNLLGVKYYKGLLDN
>Mature_336_residues
SEEKSVLRDLNLKNDDELMLRPQTLNQYIGQDDIKEMLSIYIQAALKREESLDHVLLYGAPGLGKTTLAQIIANELGVDI
KITSGPAIEKTGDLVALLSSLSPGDVLFIDEIHRIPRFVEEVLYSAMEDYTLDIVLDKERDSRSIRIELPPFTLIGATTR
FGDLSHPLRERFGAVFRLSYYKLEEIKQIVRRTSKVYQNEIDEKAVDELSKRSRGTPRIANRLFRRVRDFAEIMTDAVIT
LDITQLALTKLGIDHKGLDASDYLYLRGIVERFNGGPVGLESLASTIGEEPGTIEDVYEPYLLQEGYIKRTPRGRVATEL
AYNLLGVKYYKGLLDN

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:
GO:0000166: Holliday junction ATP-dependent DNA helicase RuvB
GO:0003677: Holliday junction ATP-dependent DNA helicase RuvB
GO:0004386: Holliday junction ATP-dependent DNA helicase RuvB
GO:0005524: Holliday junction ATP-dependent DNA helicase RuvB
GO:0006281: Holliday junction ATP-dependent DNA helicase RuvB
GO:0006310: Holliday junction ATP-dependent DNA helicase RuvB
GO:0006974: Holliday junction ATP-dependent DNA helicase RuvB
GO:0009378: Holliday junction ATP-dependent DNA helicase RuvB
GO:0009432: Holliday junction ATP-dependent DNA helicase RuvB
GO:0016787: Holliday junction ATP-dependent DNA helicase RuvB
GO:0017111: Holliday junction ATP-dependent DNA helicase RuvB

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=308, Percent_Identity=54.2207792207792, Blast_Score=353, Evalue=1e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_ACHLI (A9NF62)

Other databases:

- EMBL:   CP000896
- RefSeq:   YP_001620368.1
- ProteinModelPortal:   A9NF62
- SMR:   A9NF62
- GeneID:   5804368
- GenomeReviews:   CP000896_GR
- KEGG:   acl:ACL_0370
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- ProtClustDB:   PRK00080
- BioCyc:   ALAI441768:ACL_0370-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 38143; Mature: 38012

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEKSVLRDLNLKNDDELMLRPQTLNQYIGQDDIKEMLSIYIQAALKREESLDHVLLYG
CCCHHHHHHHCCCCCCCCEEECHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
APGLGKTTLAQIIANELGVDIKITSGPAIEKTGDLVALLSSLSPGDVLFIDEIHRIPRFV
CCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHH
EEVLYSAMEDYTLDIVLDKERDSRSIRIELPPFTLIGATTRFGDLSHPLRERFGAVFRLS
HHHHHHHHHCCEEEEEEECCCCCCEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHH
YYKLEEIKQIVRRTSKVYQNEIDEKAVDELSKRSRGTPRIANRLFRRVRDFAEIMTDAVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
TLDITQLALTKLGIDHKGLDASDYLYLRGIVERFNGGPVGLESLASTIGEEPGTIEDVYE
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHH
PYLLQEGYIKRTPRGRVATELAYNLLGVKYYKGLLDN
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEEKSVLRDLNLKNDDELMLRPQTLNQYIGQDDIKEMLSIYIQAALKREESLDHVLLYG
CCHHHHHHHCCCCCCCCEEECHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
APGLGKTTLAQIIANELGVDIKITSGPAIEKTGDLVALLSSLSPGDVLFIDEIHRIPRFV
CCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHH
EEVLYSAMEDYTLDIVLDKERDSRSIRIELPPFTLIGATTRFGDLSHPLRERFGAVFRLS
HHHHHHHHHCCEEEEEEECCCCCCEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHH
YYKLEEIKQIVRRTSKVYQNEIDEKAVDELSKRSRGTPRIANRLFRRVRDFAEIMTDAVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
TLDITQLALTKLGIDHKGLDASDYLYLRGIVERFNGGPVGLESLASTIGEEPGTIEDVYE
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHH
PYLLQEGYIKRTPRGRVATELAYNLLGVKYYKGLLDN
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA