| Definition | Acholeplasma laidlawii PG-8A chromosome, complete genome. |
|---|---|
| Accession | NC_010163 |
| Length | 1,496,992 |
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The map label for this gene is polA [H]
Identifier: 162447216
GI number: 162447216
Start: 365757
End: 368399
Strand: Direct
Name: polA [H]
Synonym: ACL_0350
Alternate gene names: 162447216
Gene position: 365757-368399 (Clockwise)
Preceding gene: 162447215
Following gene: 162447217
Centisome position: 24.43
GC content: 32.31
Gene sequence:
>2643_bases ATGAAAAAAATTACACTTGTAGACGGTAACTCGATACTTTTTAGAGCATATAATGCGACTGCATATCCAGGAGCTAAGGT CTTACAAACATCCACAGGTATTTATACAAATGCCGTTTTTGCATTTAGTGGTATGATCGATAAAATTATTGAGATATCTA ATTCACATATGCTTATAGCATTTGATACTGGAGAACCGACACACAGACATAAATCATATGATGATTATAAAGCAGGTAGA AAAGAAATGCCTGAGGAATTAGGTAGTCAAATACCTTTAATCCATGAACTTATTAAGTATCTTGGCATTAAAGAATACTC CATGGCTGGTTATGAAGCAGATGACATCATTGGTACCCTTGCTAAAATGGCTGAAAAAGCTGGTTATAAAGTCGATGTCT TTTCATCTGATTATGACCTTTTACAATTAGTTACAGAACATATTACTGTGCACATGTTAAAAAAAGGTATGCGTGTTGTA GAAACCTACACACCTGAAACTTTAAAAGAAACTTATGGTTTTTCTCATGAGTTATTTATTGATTTTAAAGCATTAGTTGG TGATAAATCTGATAATATTCCAGGTGTTCCAGGCGTTGGAGAAGTCACTGCTAAAAAGTTAATTGCTGAATATGGTACGT TAGAAAATATTTTAAATAATGCAGAACATATTAAAGGTAAGCTTGGAGAAAACATTCGTACAAACAAAGAACAGGCAGTT TTTTCAAAAGAACTCTCAACAATTCTAACGGATATGCCACTACCTTTTGATCTTGAAGATACAAAAGTTGGTGAAGTAGA TGAAGAAAGCTTAATTAATTTTTATAAACGTCTTGAATTAAAACAATTAGTGATATCTTATAATAAGAAAAATCCAGAAG TTGCTGCAACTACTGCCGAAACTTCTGAGTTTCAATATAAAGCAATTGATTCTGAAAATGCATTAAAATCTCTACTCAAC CAAGATCTTGCAATTTACTTTGAATTTTCAGATTTCAATTACCACAAAGCTGAACTTTGGGGTGTTGGTTTAAGTAATGG AAAAATCAATTACTTTGTAGATAGTGAAACGTTTTTAAATAGTGCCTCATTTAAAGCTTATTTAGAAAGTTCTAAATATA AAAAGTATACATATCACTATAAAGGTACAAAAGTATTTTTAAAGAAACTTGGTACCGAGTTTAATCATGTAACCTATGAT TTACTTTTAGCTGCTTACTTAATTCAGTCCAGTATTGGTAAACAAGACTTTACCTTTATTGCGCAACAATTTCAAATCAC GGATATTCAATATGATGAAGAAGTTTATGGTAAAGGTGCTAAAAAGGCCCTACCTTTAATTCCATCAGACTATGAAGGTC ATATAGCTAAAAAGGCGTATATCATCCATAAACTGATGCAACAAACACTAGATGTATTAACTGAACGTAACCAATTAAGT TTACTAAATGATGTGGAACTACCTCTATCAGATGTCCTTGCAGATATGGAATATCAAGGTTTAACAGTTGACCTAAAAGA ACTAGAAAATCAAACTAAAGATATGTCTTTACGTATTGATAAATTAAGAACTGAAATTCTTTTGCTAGCAGGTGTTGACT TTAATGTCGATAGTCCTAAACAATTAGGTGATGTCTTATTTGATACCTTAGGTCTACCAAATGGTAAGAAAACTAAAACA GGATACTCTACAGGTATTGAAGTTTTAAACAACCTAATCGATCACCATCCAATTATTAACTTAATTATTGAATATAGACA ACTAACTAAACTGTATTCAACCTATTTAATCGGTATTAAAGAATCCGTATTTGAAGATAACCGTGTACACACCATTTATA ATCAAGCACTAACCTTAACTGGTCGCTTATCAAGTTTAGAACCGAACTTACAAAATATTCCTATTCGTACAGAAGAAGGT AGACAAATAAGAAAATTATTTATTCCTGAACCTGATAGCTATTTTGTAGGTGCTGACTACTCTCAAATCGAATTACGTGT GTTAGCAGCTATGGCTGATGTTAAAAATTTAAAACAAGCATTTGAAGAAGATCAAGACATTCATACCGTAACAGCTCAAA AGGTATTCCACGTAGATACTGTCGATAGTGAACAAAGAAGACGTGCTAAAGCAGTTAACTTCGGTATTATTTATGGTATT GGACCATGGAGTTTATCTGAGGATATTGGTGTCACGGTGAAAGAAGCAGAAAACTTTATTAGCCGTTATTTGGAAGTATA TCCTGAAATTAAAGATTACATGACCAATATCGTTGAGTTTGCTAAAACACATGGCTATGTTGAAACACTTTTAAATCGTC GCCGTTATATACCAGAACTATCCTCTAAAGTATTTAACTTAAGAGAGTTTGGTAAACGTACCAGTTTAAATGCGCCGATT CAAGGTACAGCAGCAGACATTATTAAACTCGCGATGATTGATTTACACAACTATTTAAAAGATAATAAAAAGAAATCAAA ACTTATCCTACAAGTACATGACGAACTCATCGTTGAGGTTGTTAAAGAAGAGTTGGATGAGATGAAAGAAGTCATACCAA ACATCATGGAAAAAGCCTTTAATTTAGGCGTTAGACTGAAAACATCATGTGATGTTGGAAACAACTGGTATGAATTAAAA TAA
Upstream 100 bases:
>100_bases GGATTCTACCTTTCATAATATAGGGATAAGTTTCCCACATTTTTTGTGGAAAACTTATCTAGATAACTATGTTAAAATAC ATTTATAGAAGGGGTACAAT
Downstream 100 bases:
>100_bases AGGAGAAGTACTATGCCTGAACTACCTGAGGTAGAAACCATTCGTAGAAATTTAAATACAGTATTAGAAGGTGAAATCAT ACGTGAGGTTTTAGTATACT
Product: DNA polymerase I
Products: NA
Alternate protein names: POL I [H]
Number of amino acids: Translated: 880; Mature: 880
Protein sequence:
>880_residues MKKITLVDGNSILFRAYNATAYPGAKVLQTSTGIYTNAVFAFSGMIDKIIEISNSHMLIAFDTGEPTHRHKSYDDYKAGR KEMPEELGSQIPLIHELIKYLGIKEYSMAGYEADDIIGTLAKMAEKAGYKVDVFSSDYDLLQLVTEHITVHMLKKGMRVV ETYTPETLKETYGFSHELFIDFKALVGDKSDNIPGVPGVGEVTAKKLIAEYGTLENILNNAEHIKGKLGENIRTNKEQAV FSKELSTILTDMPLPFDLEDTKVGEVDEESLINFYKRLELKQLVISYNKKNPEVAATTAETSEFQYKAIDSENALKSLLN QDLAIYFEFSDFNYHKAELWGVGLSNGKINYFVDSETFLNSASFKAYLESSKYKKYTYHYKGTKVFLKKLGTEFNHVTYD LLLAAYLIQSSIGKQDFTFIAQQFQITDIQYDEEVYGKGAKKALPLIPSDYEGHIAKKAYIIHKLMQQTLDVLTERNQLS LLNDVELPLSDVLADMEYQGLTVDLKELENQTKDMSLRIDKLRTEILLLAGVDFNVDSPKQLGDVLFDTLGLPNGKKTKT GYSTGIEVLNNLIDHHPIINLIIEYRQLTKLYSTYLIGIKESVFEDNRVHTIYNQALTLTGRLSSLEPNLQNIPIRTEEG RQIRKLFIPEPDSYFVGADYSQIELRVLAAMADVKNLKQAFEEDQDIHTVTAQKVFHVDTVDSEQRRRAKAVNFGIIYGI GPWSLSEDIGVTVKEAENFISRYLEVYPEIKDYMTNIVEFAKTHGYVETLLNRRRYIPELSSKVFNLREFGKRTSLNAPI QGTAADIIKLAMIDLHNYLKDNKKKSKLILQVHDELIVEVVKEELDEMKEVIPNIMEKAFNLGVRLKTSCDVGNNWYELK
Sequences:
>Translated_880_residues MKKITLVDGNSILFRAYNATAYPGAKVLQTSTGIYTNAVFAFSGMIDKIIEISNSHMLIAFDTGEPTHRHKSYDDYKAGR KEMPEELGSQIPLIHELIKYLGIKEYSMAGYEADDIIGTLAKMAEKAGYKVDVFSSDYDLLQLVTEHITVHMLKKGMRVV ETYTPETLKETYGFSHELFIDFKALVGDKSDNIPGVPGVGEVTAKKLIAEYGTLENILNNAEHIKGKLGENIRTNKEQAV FSKELSTILTDMPLPFDLEDTKVGEVDEESLINFYKRLELKQLVISYNKKNPEVAATTAETSEFQYKAIDSENALKSLLN QDLAIYFEFSDFNYHKAELWGVGLSNGKINYFVDSETFLNSASFKAYLESSKYKKYTYHYKGTKVFLKKLGTEFNHVTYD LLLAAYLIQSSIGKQDFTFIAQQFQITDIQYDEEVYGKGAKKALPLIPSDYEGHIAKKAYIIHKLMQQTLDVLTERNQLS LLNDVELPLSDVLADMEYQGLTVDLKELENQTKDMSLRIDKLRTEILLLAGVDFNVDSPKQLGDVLFDTLGLPNGKKTKT GYSTGIEVLNNLIDHHPIINLIIEYRQLTKLYSTYLIGIKESVFEDNRVHTIYNQALTLTGRLSSLEPNLQNIPIRTEEG RQIRKLFIPEPDSYFVGADYSQIELRVLAAMADVKNLKQAFEEDQDIHTVTAQKVFHVDTVDSEQRRRAKAVNFGIIYGI GPWSLSEDIGVTVKEAENFISRYLEVYPEIKDYMTNIVEFAKTHGYVETLLNRRRYIPELSSKVFNLREFGKRTSLNAPI QGTAADIIKLAMIDLHNYLKDNKKKSKLILQVHDELIVEVVKEELDEMKEVIPNIMEKAFNLGVRLKTSCDVGNNWYELK >Mature_880_residues MKKITLVDGNSILFRAYNATAYPGAKVLQTSTGIYTNAVFAFSGMIDKIIEISNSHMLIAFDTGEPTHRHKSYDDYKAGR KEMPEELGSQIPLIHELIKYLGIKEYSMAGYEADDIIGTLAKMAEKAGYKVDVFSSDYDLLQLVTEHITVHMLKKGMRVV ETYTPETLKETYGFSHELFIDFKALVGDKSDNIPGVPGVGEVTAKKLIAEYGTLENILNNAEHIKGKLGENIRTNKEQAV FSKELSTILTDMPLPFDLEDTKVGEVDEESLINFYKRLELKQLVISYNKKNPEVAATTAETSEFQYKAIDSENALKSLLN QDLAIYFEFSDFNYHKAELWGVGLSNGKINYFVDSETFLNSASFKAYLESSKYKKYTYHYKGTKVFLKKLGTEFNHVTYD LLLAAYLIQSSIGKQDFTFIAQQFQITDIQYDEEVYGKGAKKALPLIPSDYEGHIAKKAYIIHKLMQQTLDVLTERNQLS LLNDVELPLSDVLADMEYQGLTVDLKELENQTKDMSLRIDKLRTEILLLAGVDFNVDSPKQLGDVLFDTLGLPNGKKTKT GYSTGIEVLNNLIDHHPIINLIIEYRQLTKLYSTYLIGIKESVFEDNRVHTIYNQALTLTGRLSSLEPNLQNIPIRTEEG RQIRKLFIPEPDSYFVGADYSQIELRVLAAMADVKNLKQAFEEDQDIHTVTAQKVFHVDTVDSEQRRRAKAVNFGIIYGI GPWSLSEDIGVTVKEAENFISRYLEVYPEIKDYMTNIVEFAKTHGYVETLLNRRRYIPELSSKVFNLREFGKRTSLNAPI QGTAADIIKLAMIDLHNYLKDNKKKSKLILQVHDELIVEVVKEELDEMKEVIPNIMEKAFNLGVRLKTSCDVGNNWYELK
Specific function: In addition to polymerase activity, this DNA polymerase exhibits 5' to 3' exonuclease activity [H]
COG id: COG0749
COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains
Gene ontology:
GO:0003676: DNA polymerase
GO:0003677: DNA polymerase
GO:0003824: DNA polymerase
GO:0003887: DNA polymerase
GO:0005622: DNA polymerase
GO:0006139: DNA polymerase
GO:0006260: DNA polymerase
GO:0006281: DNA polymerase
GO:0008408: DNA polymerase
GO:0008409: DNA polymerase
GO:0016740: DNA polymerase
GO:0016779: DNA polymerase
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 5'-3' exonuclease domain [H]
Homologues:
Organism=Homo sapiens, GI112420958, Length=519, Percent_Identity=28.7090558766859, Blast_Score=206, Evalue=8e-53, Organism=Homo sapiens, GI139394648, Length=539, Percent_Identity=27.8293135435993, Blast_Score=201, Evalue=3e-51, Organism=Escherichia coli, GI1790294, Length=932, Percent_Identity=36.1587982832618, Blast_Score=504, Evalue=1e-143, Organism=Escherichia coli, GI226510970, Length=223, Percent_Identity=29.1479820627803, Blast_Score=105, Evalue=9e-24, Organism=Caenorhabditis elegans, GI86563272, Length=405, Percent_Identity=29.3827160493827, Blast_Score=174, Evalue=2e-43, Organism=Drosophila melanogaster, GI17933644, Length=467, Percent_Identity=30.8351177730193, Blast_Score=196, Evalue=9e-50,
Paralogues:
None
Copy number: 400 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002562 - InterPro: IPR020046 - InterPro: IPR020045 - InterPro: IPR002421 - InterPro: IPR020047 - InterPro: IPR019760 - InterPro: IPR001098 - InterPro: IPR018320 - InterPro: IPR002298 - InterPro: IPR008918 - InterPro: IPR003583 - InterPro: IPR012337 [H]
Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]
EC number: =2.7.7.7 [H]
Molecular weight: Translated: 100087; Mature: 100087
Theoretical pI: Translated: 5.44; Mature: 5.44
Prosite motif: PS00447 DNA_POLYMERASE_A
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.1 %Cys (Translated Protein) 1.9 %Met (Translated Protein) 2.0 %Cys+Met (Translated Protein) 0.1 %Cys (Mature Protein) 1.9 %Met (Mature Protein) 2.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKKITLVDGNSILFRAYNATAYPGAKVLQTSTGIYTNAVFAFSGMIDKIIEISNSHMLIA CCEEEEEECCEEEEEEECCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEE FDTGEPTHRHKSYDDYKAGRKEMPEELGSQIPLIHELIKYLGIKEYSMAGYEADDIIGTL ECCCCCCCCCCCCCHHHHHHHHCHHHHCCCCCHHHHHHHHHCCCHHHCCCCCHHHHHHHH AKMAEKAGYKVDVFSSDYDLLQLVTEHITVHMLKKGMRVVETYTPETLKETYGFSHELFI HHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHCCCCEEEEE DFKALVGDKSDNIPGVPGVGEVTAKKLIAEYGTLENILNNAEHIKGKLGENIRTNKEQAV EHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHH FSKELSTILTDMPLPFDLEDTKVGEVDEESLINFYKRLELKQLVISYNKKNPEVAATTAE HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC TSEFQYKAIDSENALKSLLNQDLAIYFEFSDFNYHKAELWGVGLSNGKINYFVDSETFLN CCCEEEEECCCHHHHHHHHCCCEEEEEEECCCCCEEHEEEEEECCCCEEEEEECCHHHHC SASFKAYLESSKYKKYTYHYKGTKVFLKKLGTEFNHVTYDLLLAAYLIQSSIGKQDFTFI CHHHHHHHHCCCCEEEEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH AQQFQITDIQYDEEVYGKGAKKALPLIPSDYEGHIAKKAYIIHKLMQQTLDVLTERNQLS HHHHEEECCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHH LLNDVELPLSDVLADMEYQGLTVDLKELENQTKDMSLRIDKLRTEILLLAGVDFNVDSPK HHHCCCCCHHHHHHCCCCCCCEEEHHHHHCCHHHHHHHHHHHHEEEEEEECCCCCCCCHH QLGDVLFDTLGLPNGKKTKTGYSTGIEVLNNLIDHHPIINLIIEYRQLTKLYSTYLIGIK HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH ESVFEDNRVHTIYNQALTLTGRLSSLEPNLQNIPIRTEEGRQIRKLFIPEPDSYFVGADY HHHCCCCCEEEEHHHHHEEEECHHCCCCCCCCCCCCCCCCCHHHHEECCCCCCEEECCCC SQIELRVLAAMADVKNLKQAFEEDQDIHTVTAQKVFHVDTVDSEQRRRAKAVNFGIIYGI HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHEEEEECCCHHHHHHHHHCCEEEEEEC GPWSLSEDIGVTVKEAENFISRYLEVYPEIKDYMTNIVEFAKTHGYVETLLNRRRYIPEL CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH SSKVFNLREFGKRTSLNAPIQGTAADIIKLAMIDLHNYLKDNKKKSKLILQVHDELIVEV HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH VKEELDEMKEVIPNIMEKAFNLGVRLKTSCDVGNNWYELK HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECC >Mature Secondary Structure MKKITLVDGNSILFRAYNATAYPGAKVLQTSTGIYTNAVFAFSGMIDKIIEISNSHMLIA CCEEEEEECCEEEEEEECCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEE FDTGEPTHRHKSYDDYKAGRKEMPEELGSQIPLIHELIKYLGIKEYSMAGYEADDIIGTL ECCCCCCCCCCCCCHHHHHHHHCHHHHCCCCCHHHHHHHHHCCCHHHCCCCCHHHHHHHH AKMAEKAGYKVDVFSSDYDLLQLVTEHITVHMLKKGMRVVETYTPETLKETYGFSHELFI HHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHCCCCEEEEE DFKALVGDKSDNIPGVPGVGEVTAKKLIAEYGTLENILNNAEHIKGKLGENIRTNKEQAV EHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHH FSKELSTILTDMPLPFDLEDTKVGEVDEESLINFYKRLELKQLVISYNKKNPEVAATTAE HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC TSEFQYKAIDSENALKSLLNQDLAIYFEFSDFNYHKAELWGVGLSNGKINYFVDSETFLN CCCEEEEECCCHHHHHHHHCCCEEEEEEECCCCCEEHEEEEEECCCCEEEEEECCHHHHC SASFKAYLESSKYKKYTYHYKGTKVFLKKLGTEFNHVTYDLLLAAYLIQSSIGKQDFTFI CHHHHHHHHCCCCEEEEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH AQQFQITDIQYDEEVYGKGAKKALPLIPSDYEGHIAKKAYIIHKLMQQTLDVLTERNQLS HHHHEEECCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHH LLNDVELPLSDVLADMEYQGLTVDLKELENQTKDMSLRIDKLRTEILLLAGVDFNVDSPK HHHCCCCCHHHHHHCCCCCCCEEEHHHHHCCHHHHHHHHHHHHEEEEEEECCCCCCCCHH QLGDVLFDTLGLPNGKKTKTGYSTGIEVLNNLIDHHPIINLIIEYRQLTKLYSTYLIGIK HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH ESVFEDNRVHTIYNQALTLTGRLSSLEPNLQNIPIRTEEGRQIRKLFIPEPDSYFVGADY HHHCCCCCEEEEHHHHHEEEECHHCCCCCCCCCCCCCCCCCHHHHEECCCCCCEEECCCC SQIELRVLAAMADVKNLKQAFEEDQDIHTVTAQKVFHVDTVDSEQRRRAKAVNFGIIYGI HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHEEEEECCCHHHHHHHHHCCEEEEEEC GPWSLSEDIGVTVKEAENFISRYLEVYPEIKDYMTNIVEFAKTHGYVETLLNRRRYIPEL CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH SSKVFNLREFGKRTSLNAPIQGTAADIIKLAMIDLHNYLKDNKKKSKLILQVHDELIVEV HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH VKEELDEMKEVIPNIMEKAFNLGVRLKTSCDVGNNWYELK HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 7557480; 9016716; 9440698 [H]