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The map label for this gene is prfB

Identifier: 162447197

GI number: 162447197

Start: 343070

End: 344167

Strand: Direct

Name: prfB

Synonym: ACL_0331

Alternate gene names: 162447197

Gene position: 343070-344167 (Clockwise)

Preceding gene: 162447196

Following gene: 162447198

Centisome position: 22.92

GC content: 33.06

Gene sequence:

>1098_bases
ATGGAAAAGTACGAAGTAAATAAGACACTAGAACTTTTTGAAACAAAAATTAAAGATTTAGAAAATGCTTTAGATTTAGA
TGCTATTAATAACAGATTAAAAGAAATCGAACCCATCATGGCAAATCCTAATTTTTGGAATGATTCAAATCAAGCAAAAA
AGATTAGCCAAGAATTAAATCAACTTACAGAGAAAAAACAAACTATTACATCGATTCAAAACCAATATGAGGATGCCTTA
ATGTGGCTTGAAGAAGCCAAAGAAGGTACTGAGAGTTGGGATATTTTAGAAGCTGAAATTGATTCACTTCAAAAAAAGAT
ATCTGAGTTTGAAATTGAAGTATTACTCAATGGAGAATATGATCATAACAATGCAATCTTAGAACTTCATCCAGGTGCTG
GTGGTACAGAGTCCATGGACTGGTGTGGTATTTTAATGCGTATGTATGAACGCTTTGCTGGCTATAAAGGTTATAAGGTA
GAAATACTAAACTACTTAGCGGGTGAAGAAGCAGGTGTTAAATCTGTTACATTACGTATTTCTGGTCCATATGCTTATGG
TAATTTAAAGTCTGAACGTGGTGTTCACAGATTAGTTCGTATCTCACCATTTGACTCCAATAAAAGAAGACATACTTCCT
TTGTTTCATGTGATGTAGCACCTGAAATTGATGAAACAAGTGAAGTTGAATTAAAAGATGATGATATTCGTATGGATACA
TTCCAATCCTCTGGTGCAGGTGGTCAGTCGGTTAACACAACTTACTCTGCTGTAAGATTAACACATATACCAACTGGTAT
TGTTGTTAATATTCAAAATGAACGTAGTCAGATTAAGAATAAAGAAGCAGCAATGCAAATTTTAAAATCTAAACTGATAC
AAAAAGAACTAGAAGAAAAACAAGCGAAGTTAAATGCCCTTAAGGGTGAAAAATCAGATATTGGTTGGGGTAGTCAAATT
AGATCTTATGTATTCCAACCTTATCAAATGGTCAAAGACCACAGAACTAATTATGAAGTTGGTAATATTCAGTCTGTCAT
GGATGGAGATATTGACGGTTTCATTAATGCTTATTTAAAGAGTAAAGCCTATGAATAA

Upstream 100 bases:

>100_bases
AAAATACAAGCACAAACCAAGGTGAAGATGCTTCTAAACAAAAACGTAAACAACCTAAGCGTGCTCACAGAAGTCAATTA
CCGTGGAATAGAAGGTAGTT

Downstream 100 bases:

>100_bases
AGAAATGATTAAATCATATGCACTCATTACTCTAGGCGTATTTTTACTACATTTAGCATTTTACTTTTTTATGCAACCAA
TGGGTTTAATCACAGGTGGT

Product: peptide chain release factor RF-2

Products: NA

Alternate protein names: RF-2

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MEKYEVNKTLELFETKIKDLENALDLDAINNRLKEIEPIMANPNFWNDSNQAKKISQELNQLTEKKQTITSIQNQYEDAL
MWLEEAKEGTESWDILEAEIDSLQKKISEFEIEVLLNGEYDHNNAILELHPGAGGTESMDWCGILMRMYERFAGYKGYKV
EILNYLAGEEAGVKSVTLRISGPYAYGNLKSERGVHRLVRISPFDSNKRRHTSFVSCDVAPEIDETSEVELKDDDIRMDT
FQSSGAGGQSVNTTYSAVRLTHIPTGIVVNIQNERSQIKNKEAAMQILKSKLIQKELEEKQAKLNALKGEKSDIGWGSQI
RSYVFQPYQMVKDHRTNYEVGNIQSVMDGDIDGFINAYLKSKAYE

Sequences:

>Translated_365_residues
MEKYEVNKTLELFETKIKDLENALDLDAINNRLKEIEPIMANPNFWNDSNQAKKISQELNQLTEKKQTITSIQNQYEDAL
MWLEEAKEGTESWDILEAEIDSLQKKISEFEIEVLLNGEYDHNNAILELHPGAGGTESMDWCGILMRMYERFAGYKGYKV
EILNYLAGEEAGVKSVTLRISGPYAYGNLKSERGVHRLVRISPFDSNKRRHTSFVSCDVAPEIDETSEVELKDDDIRMDT
FQSSGAGGQSVNTTYSAVRLTHIPTGIVVNIQNERSQIKNKEAAMQILKSKLIQKELEEKQAKLNALKGEKSDIGWGSQI
RSYVFQPYQMVKDHRTNYEVGNIQSVMDGDIDGFINAYLKSKAYE
>Mature_365_residues
MEKYEVNKTLELFETKIKDLENALDLDAINNRLKEIEPIMANPNFWNDSNQAKKISQELNQLTEKKQTITSIQNQYEDAL
MWLEEAKEGTESWDILEAEIDSLQKKISEFEIEVLLNGEYDHNNAILELHPGAGGTESMDWCGILMRMYERFAGYKGYKV
EILNYLAGEEAGVKSVTLRISGPYAYGNLKSERGVHRLVRISPFDSNKRRHTSFVSCDVAPEIDETSEVELKDDDIRMDT
FQSSGAGGQSVNTTYSAVRLTHIPTGIVVNIQNERSQIKNKEAAMQILKSKLIQKELEEKQAKLNALKGEKSDIGWGSQI
RSYVFQPYQMVKDHRTNYEVGNIQSVMDGDIDGFINAYLKSKAYE

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:
GO:0003747: Peptide chain release factor 2
GO:0005737: Peptide chain release factor 2
GO:0006412: Peptide chain release factor 2
GO:0006415: Peptide chain release factor 2
GO:0016149: Peptide chain release factor 2

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=278, Percent_Identity=37.7697841726619, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI34577120, Length=253, Percent_Identity=41.1067193675889, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI166795305, Length=175, Percent_Identity=37.7142857142857, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI2367172, Length=358, Percent_Identity=45.8100558659218, Blast_Score=317, Evalue=7e-88,
Organism=Escherichia coli, GI1787462, Length=344, Percent_Identity=34.593023255814, Blast_Score=194, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=309, Percent_Identity=30.42071197411, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321295, Length=251, Percent_Identity=39.0438247011952, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI19921226, Length=261, Percent_Identity=34.8659003831418, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF2_ACHLI (A9NF23)

Other databases:

- EMBL:   CP000896
- RefSeq:   YP_001620329.1
- ProteinModelPortal:   A9NF23
- SMR:   A9NF23
- GeneID:   5804308
- GenomeReviews:   CP000896_GR
- KEGG:   acl:ACL_0331
- HOGENOM:   HBG629764
- OMA:   TEMLLRM
- BioCyc:   ALAI441768:ACL_0331-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00094
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374
- PANTHER:   PTHR11075:SF6
- SMART:   SM00937
- TIGRFAMs:   TIGR00020

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41587; Mature: 41587

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKYEVNKTLELFETKIKDLENALDLDAINNRLKEIEPIMANPNFWNDSNQAKKISQELN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
QLTEKKQTITSIQNQYEDALMWLEEAKEGTESWDILEAEIDSLQKKISEFEIEVLLNGEY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEEEECCC
DHNNAILELHPGAGGTESMDWCGILMRMYERFAGYKGYKVEILNYLAGEEAGVKSVTLRI
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEEEE
SGPYAYGNLKSERGVHRLVRISPFDSNKRRHTSFVSCDVAPEIDETSEVELKDDDIRMDT
ECCEEECCCCHHCCHHHEEEECCCCCCCCCCCCEEEECCCCCCCCCCCEEECCCCEEEEC
FQSSGAGGQSVNTTYSAVRLTHIPTGIVVNIQNERSQIKNKEAAMQILKSKLIQKELEEK
CCCCCCCCCCCCCHHHEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAKLNALKGEKSDIGWGSQIRSYVFQPYQMVKDHRTNYEVGNIQSVMDGDIDGFINAYLK
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHH
SKAYE
HCCCC
>Mature Secondary Structure
MEKYEVNKTLELFETKIKDLENALDLDAINNRLKEIEPIMANPNFWNDSNQAKKISQELN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
QLTEKKQTITSIQNQYEDALMWLEEAKEGTESWDILEAEIDSLQKKISEFEIEVLLNGEY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEEEECCC
DHNNAILELHPGAGGTESMDWCGILMRMYERFAGYKGYKVEILNYLAGEEAGVKSVTLRI
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEEEE
SGPYAYGNLKSERGVHRLVRISPFDSNKRRHTSFVSCDVAPEIDETSEVELKDDDIRMDT
ECCEEECCCCHHCCHHHEEEECCCCCCCCCCCCEEEECCCCCCCCCCCEEECCCCEEEEC
FQSSGAGGQSVNTTYSAVRLTHIPTGIVVNIQNERSQIKNKEAAMQILKSKLIQKELEEK
CCCCCCCCCCCCCHHHEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAKLNALKGEKSDIGWGSQIRSYVFQPYQMVKDHRTNYEVGNIQSVMDGDIDGFINAYLK
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHH
SKAYE
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA