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The map label for this gene is infB [H]

Identifier: 162447186

GI number: 162447186

Start: 328213

End: 330075

Strand: Direct

Name: infB [H]

Synonym: ACL_0320

Alternate gene names: 162447186

Gene position: 328213-330075 (Clockwise)

Preceding gene: 162447185

Following gene: 162447187

Centisome position: 21.92

GC content: 35.53

Gene sequence:

>1863_bases
ATGGCAAAACAAAGAAGTAATAATAAAAATAACAATAACACTAAGAGACCAAGTAAAAATAATTTTGGAAAGTCTACTTA
TTCAAACACTCAAAAACCAGCACCTAAAAAAGTGATAACAGAGTTAATTTTCAAACCTGAAATGACGGTTTCAGATGTGG
CAGAAGCACTTGACATCTCAAACGCAGTTTTAATTAAAAAACTAATGGGTTTAGGGATGATGACTTCTGTAAACCAAGTT
TTAGAACGTGATGTTGTAGAACTTATAGCTATGGATATGGGTATTGAAGTTAAAGATGAAGTAATCACAGATTTAACAAG
ATATGATGAAATGGAAATTATTGACGATCCAAAAGATCTTGTTAAAAGAAGTCCAATTATTACGATTATGGGTCACGTAG
ACCATGGTAAAACAACACTTTTAGATTCTATTAGAAAATCTAGAGTGGTCACAGGAGAAGCTGGTGGAATCACACAACAC
ATCGGTGCTTATCAAGTTGAACATGGTGGTGAAAAAATTACATTTATCGATACCCCAGGTCACGCGGCATTTACTGAAAT
GCGTGCACGTGGAGCTAAAGTAACTGATATTGTTGTTTTAGTTGTGGCTGCAGATGATGGTGTTAAACCACAAACAATTG
AAGCGTTACAACATGCCAAGGCATCAGGCGTTCCTATTATCGTAGCAATCAATAAAATTGATAAACCAACAGCAAATCCA
GATAACGTTATGAGTGCTTTATCAGCACATGATTTAACGCCTGAAGCATGGGGTGGTACAACACCATATGTTGAAGTATC
TGCATTAAAACGTTTAGGTATTGATAATTTATTAGATATTATTTTAGTATTAGCAGAAGTTGAAGACTTAAAAGCAAATC
CAAAACGTGATGCAATGGGTACTGTTATTGAAGCTTACCTAGACAAAGGTAGAGGTCCGGTTGCAACAGTTATTGTTGAA
AATGGTACTTTACGTATTGGTGATATCATCGTTATTGGTAATACATTCGGTCGTATACGTACAATGAATGATGATCTTAA
AAAACGTTATGATGAAGCACTTCCAGGTACACCGGTTGAAATTACAGGTTTAGACCAAGTACCTCAAGCTGGTGATATTT
TCATGGTATTTAAAGATGAAAGAGTAGCACGCCAAACAGCGGAAGCACGTTCTTCAAAACTAAAAGAAACATATATTAAA
CAACAAAAAGCGAGTTCACTTGAATCCATGTTTGGTGCACAAGATGATGGCGAAAAAGTATTAAACTTAGTACTTAAATG
TGACGTTCAAGGTAGTATTGAAGCATTAAAAGGTATGCTCGATAAAATTGATGTTGATGGCTTCAAAGCCAACATCGTGC
GTAGTGGTGTGGGTGGAATTTCTGAAACAGATGTTCAACTAGCATCTGCATCTGGTGCAGTCGTTATTGGTTTTAACGTT
CGTCCTACAGCTGCAGTAAAATCTCTAGCAGAATCTATGAATGTTGAAATCAGATTATATAATGTCGTATATCGTATTAC
TGAAGACATCGAAAAAGCTCTAAAAGGTATGTTAGAACCAGTCTTTGAAGATGTTGTAACTGGTACCGCAGAAGTACGTC
AATTATTTAAAATTTCTAAAATTGGAACAATCGCAGGTAGCTATGTTACAAACGGTGTCATCCATAGAGATTCTAAAGTT
ACAGTCATTAGAGAAGGTGTTGTTATCTATAACGGAGTACTATCTAGTTTAAAACGTGGTAAAGATGATGTTAAAGAAGT
ACGTCAAGGATTTGAATTCGGCTTTAGCGTTGAAAACTACAACGATTTAAAAGAAGGTGACGTGATTGAAGCGTCTATTG
AAAGAGAAGTGGAGGTAAATTAA

Upstream 100 bases:

>100_bases
ACTAGATATTTCTAGTGCCATAGGTAAGAAAAATATTAAAGTCATAGGAATAAAGGATAAAGGGTTTAGTATGATGCTTA
AATAGAAAGGTTGATGCAAT

Downstream 100 bases:

>100_bases
TTATGAGTATTAATGTCGATCGTTTAGCTTCAAGAATCTTTAGAGAATTAGTTCATATCGTAAATGATGTGATTAAAAAC
ACTCAAATTGGTTATATTAC

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 620; Mature: 619

Protein sequence:

>620_residues
MAKQRSNNKNNNNTKRPSKNNFGKSTYSNTQKPAPKKVITELIFKPEMTVSDVAEALDISNAVLIKKLMGLGMMTSVNQV
LERDVVELIAMDMGIEVKDEVITDLTRYDEMEIIDDPKDLVKRSPIITIMGHVDHGKTTLLDSIRKSRVVTGEAGGITQH
IGAYQVEHGGEKITFIDTPGHAAFTEMRARGAKVTDIVVLVVAADDGVKPQTIEALQHAKASGVPIIVAINKIDKPTANP
DNVMSALSAHDLTPEAWGGTTPYVEVSALKRLGIDNLLDIILVLAEVEDLKANPKRDAMGTVIEAYLDKGRGPVATVIVE
NGTLRIGDIIVIGNTFGRIRTMNDDLKKRYDEALPGTPVEITGLDQVPQAGDIFMVFKDERVARQTAEARSSKLKETYIK
QQKASSLESMFGAQDDGEKVLNLVLKCDVQGSIEALKGMLDKIDVDGFKANIVRSGVGGISETDVQLASASGAVVIGFNV
RPTAAVKSLAESMNVEIRLYNVVYRITEDIEKALKGMLEPVFEDVVTGTAEVRQLFKISKIGTIAGSYVTNGVIHRDSKV
TVIREGVVIYNGVLSSLKRGKDDVKEVRQGFEFGFSVENYNDLKEGDVIEASIEREVEVN

Sequences:

>Translated_620_residues
MAKQRSNNKNNNNTKRPSKNNFGKSTYSNTQKPAPKKVITELIFKPEMTVSDVAEALDISNAVLIKKLMGLGMMTSVNQV
LERDVVELIAMDMGIEVKDEVITDLTRYDEMEIIDDPKDLVKRSPIITIMGHVDHGKTTLLDSIRKSRVVTGEAGGITQH
IGAYQVEHGGEKITFIDTPGHAAFTEMRARGAKVTDIVVLVVAADDGVKPQTIEALQHAKASGVPIIVAINKIDKPTANP
DNVMSALSAHDLTPEAWGGTTPYVEVSALKRLGIDNLLDIILVLAEVEDLKANPKRDAMGTVIEAYLDKGRGPVATVIVE
NGTLRIGDIIVIGNTFGRIRTMNDDLKKRYDEALPGTPVEITGLDQVPQAGDIFMVFKDERVARQTAEARSSKLKETYIK
QQKASSLESMFGAQDDGEKVLNLVLKCDVQGSIEALKGMLDKIDVDGFKANIVRSGVGGISETDVQLASASGAVVIGFNV
RPTAAVKSLAESMNVEIRLYNVVYRITEDIEKALKGMLEPVFEDVVTGTAEVRQLFKISKIGTIAGSYVTNGVIHRDSKV
TVIREGVVIYNGVLSSLKRGKDDVKEVRQGFEFGFSVENYNDLKEGDVIEASIEREVEVN
>Mature_619_residues
AKQRSNNKNNNNTKRPSKNNFGKSTYSNTQKPAPKKVITELIFKPEMTVSDVAEALDISNAVLIKKLMGLGMMTSVNQVL
ERDVVELIAMDMGIEVKDEVITDLTRYDEMEIIDDPKDLVKRSPIITIMGHVDHGKTTLLDSIRKSRVVTGEAGGITQHI
GAYQVEHGGEKITFIDTPGHAAFTEMRARGAKVTDIVVLVVAADDGVKPQTIEALQHAKASGVPIIVAINKIDKPTANPD
NVMSALSAHDLTPEAWGGTTPYVEVSALKRLGIDNLLDIILVLAEVEDLKANPKRDAMGTVIEAYLDKGRGPVATVIVEN
GTLRIGDIIVIGNTFGRIRTMNDDLKKRYDEALPGTPVEITGLDQVPQAGDIFMVFKDERVARQTAEARSSKLKETYIKQ
QKASSLESMFGAQDDGEKVLNLVLKCDVQGSIEALKGMLDKIDVDGFKANIVRSGVGGISETDVQLASASGAVVIGFNVR
PTAAVKSLAESMNVEIRLYNVVYRITEDIEKALKGMLEPVFEDVVTGTAEVRQLFKISKIGTIAGSYVTNGVIHRDSKVT
VIREGVVIYNGVLSSLKRGKDDVKEVRQGFEFGFSVENYNDLKEGDVIEASIEREVEVN

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family [H]

Homologues:

Organism=Homo sapiens, GI53729339, Length=547, Percent_Identity=40.4021937842779, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI53729337, Length=547, Percent_Identity=40.4021937842779, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI84043963, Length=480, Percent_Identity=28.3333333333333, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI157426893, Length=231, Percent_Identity=25.974025974026, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI34147630, Length=260, Percent_Identity=31.9230769230769, Blast_Score=67, Evalue=7e-11,
Organism=Escherichia coli, GI1789559, Length=586, Percent_Identity=50.8532423208191, Blast_Score=553, Evalue=1e-158,
Organism=Escherichia coli, GI48994988, Length=134, Percent_Identity=39.5522388059701, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI2367247, Length=226, Percent_Identity=32.7433628318584, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788922, Length=235, Percent_Identity=26.8085106382979, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790412, Length=345, Percent_Identity=28.1159420289855, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1789737, Length=345, Percent_Identity=28.1159420289855, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71994658, Length=544, Percent_Identity=37.8676470588235, Blast_Score=335, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI212656558, Length=560, Percent_Identity=27.1428571428571, Blast_Score=140, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17557151, Length=240, Percent_Identity=32.0833333333333, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=269, Percent_Identity=31.2267657992565, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25141371, Length=246, Percent_Identity=29.2682926829268, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324550, Length=602, Percent_Identity=37.375415282392, Blast_Score=361, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6319282, Length=286, Percent_Identity=32.5174825174825, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324761, Length=268, Percent_Identity=29.8507462686567, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI28572034, Length=611, Percent_Identity=36.3338788870704, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24656849, Length=575, Percent_Identity=26.2608695652174, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI160714833, Length=115, Percent_Identity=50.4347826086956, Blast_Score=112, Evalue=9e-25,
Organism=Drosophila melanogaster, GI78706572, Length=225, Percent_Identity=30.2222222222222, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19922690, Length=229, Percent_Identity=29.6943231441048, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N [H]

EC number: NA

Molecular weight: Translated: 67534; Mature: 67403

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQRSNNKNNNNTKRPSKNNFGKSTYSNTQKPAPKKVITELIFKPEMTVSDVAEALDIS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCC
NAVLIKKLMGLGMMTSVNQVLERDVVELIAMDMGIEVKDEVITDLTRYDEMEIIDDPKDL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHH
VKRSPIITIMGHVDHGKTTLLDSIRKSRVVTGEAGGITQHIGAYQVEHGGEKITFIDTPG
HHCCCEEEEEECCCCCHHHHHHHHHHHCEEECCCCCCHHHCCCEEEECCCCEEEEEECCC
HAAFTEMRARGAKVTDIVVLVVAADDGVKPQTIEALQHAKASGVPIIVAINKIDKPTANP
CHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCH
DNVMSALSAHDLTPEAWGGTTPYVEVSALKRLGIDNLLDIILVLAEVEDLKANPKRDAMG
HHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
TVIEAYLDKGRGPVATVIVENGTLRIGDIIVIGNTFGRIRTMNDDLKKRYDEALPGTPVE
HHHHHHHHCCCCCEEEEEEECCEEEEEEEEEECCCCCCEEECCHHHHHHHHHCCCCCCEE
ITGLDQVPQAGDIFMVFKDERVARQTAEARSSKLKETYIKQQKASSLESMFGAQDDGEKV
ECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LNLVLKCDVQGSIEALKGMLDKIDVDGFKANIVRSGVGGISETDVQLASASGAVVIGFNV
HHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEECCCCCEEEEECC
RPTAAVKSLAESMNVEIRLYNVVYRITEDIEKALKGMLEPVFEDVVTGTAEVRQLFKISK
CCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
IGTIAGSYVTNGVIHRDSKVTVIREGVVIYNGVLSSLKRGKDDVKEVRQGFEFGFSVENY
HHHHHHHHHHCCEEECCCEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCC
NDLKEGDVIEASIEREVEVN
CCCCCCCEEEECCCCCCCCC
>Mature Secondary Structure 
AKQRSNNKNNNNTKRPSKNNFGKSTYSNTQKPAPKKVITELIFKPEMTVSDVAEALDIS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCC
NAVLIKKLMGLGMMTSVNQVLERDVVELIAMDMGIEVKDEVITDLTRYDEMEIIDDPKDL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHH
VKRSPIITIMGHVDHGKTTLLDSIRKSRVVTGEAGGITQHIGAYQVEHGGEKITFIDTPG
HHCCCEEEEEECCCCCHHHHHHHHHHHCEEECCCCCCHHHCCCEEEECCCCEEEEEECCC
HAAFTEMRARGAKVTDIVVLVVAADDGVKPQTIEALQHAKASGVPIIVAINKIDKPTANP
CHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCH
DNVMSALSAHDLTPEAWGGTTPYVEVSALKRLGIDNLLDIILVLAEVEDLKANPKRDAMG
HHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
TVIEAYLDKGRGPVATVIVENGTLRIGDIIVIGNTFGRIRTMNDDLKKRYDEALPGTPVE
HHHHHHHHCCCCCEEEEEEECCEEEEEEEEEECCCCCCEEECCHHHHHHHHHCCCCCCEE
ITGLDQVPQAGDIFMVFKDERVARQTAEARSSKLKETYIKQQKASSLESMFGAQDDGEKV
ECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LNLVLKCDVQGSIEALKGMLDKIDVDGFKANIVRSGVGGISETDVQLASASGAVVIGFNV
HHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEECCCCCEEEEECC
RPTAAVKSLAESMNVEIRLYNVVYRITEDIEKALKGMLEPVFEDVVTGTAEVRQLFKISK
CCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
IGTIAGSYVTNGVIHRDSKVTVIREGVVIYNGVLSSLKRGKDDVKEVRQGFEFGFSVENY
HHHHHHHHHHCCEEECCCEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCC
NDLKEGDVIEASIEREVEVN
CCCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA