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The map label for this gene is pksG [H]

Identifier: 162447094

GI number: 162447094

Start: 215832

End: 216974

Strand: Direct

Name: pksG [H]

Synonym: ACL_0224

Alternate gene names: 162447094

Gene position: 215832-216974 (Clockwise)

Preceding gene: 162447092

Following gene: 162447096

Centisome position: 14.42

GC content: 32.46

Gene sequence:

>1143_bases
ATGAAAATTGGTATTCATAAAATGTCGTTTTATGTCCCAAAGTTTTACTTGGATATAAACACTTTAGCACATGCTAGAGG
TATTGACCCGTTAAAATATACAAATGGCTTACTTACACATAAAATGAGTGTAGCACCATTTAATCAAGACATCGTGACAC
TTGCAGCAAACGCAACTTTAAACTATCTAACTAAAGAAGATATCAAAGCAATCGATTTAATACTATTTACAACAGAAAGT
GGTATAGATTATTCCAAATCAGCAGCAACACATCTTATCAGTATCTTAGGTTTAAGAAATGATGTGAGAGCACTTGAGAT
GAAACAAGCGTGTTACGCTACAACTGCTGCCCTTTATTTTGCTAAAGGACATATTTTACAACATCCTGAATCTAAAGTAT
TAGTGATAGCTTCAGATATTGCAAAATATGGGTTAAATAGTTCTGGTGAACCAACTCAAGGCGCTGGTGCAGTGTCTATG
ATTGTGTCTAGAGAACCTAACATTATGGTGTTAGAAGATCAATATGGTATTCATTCAGAGGATATCTACGATTTTTGGAG
ACCAGATGGTTTAGATTATGCCCAAGTGGATGGTAAGTTCTCAAATGAAATGTATAAAAAGTTTTTCTTAAAAACATTTG
ATAACTACAAAATGCAAACCAAACATACGCTAGATGATTTTGCTGCATTAACATTCCATATACCTTATCCGAAAATTGGA
TTAAGAAGTTTAGAATTGGTTGCAGATAAAGCAAACAATCCTAGCCTATTTGAAAACTTCTATACAAGTATTTCATACAA
CAAATATATTGGTAATATCTATACAGGTTCCCTATTCTTATCTATTATTGCTTTACTTGAACAAGGTAACTTAAAAGCAG
GTGAGCGTATCGGGATGTTTGGATATGGTTCAGGTGCTGTTGCAGAGTTTTTCTCAGCAAAACTTGTTGAGGGTTATAAA
GCACATCTAAACAAAGAACTACATGAAAAACAACTTTCAAATAGAACTGAACTGGATATGGAAAAATACGCTGTATACTT
AACTCGAAAAGTTACAAATGACACTGTATTAGAAGATGATCTTGATGTTAAAGTTCAATTAAAATCAATCACAGATTATA
AGAGAAATTATCAAAATAATTAA

Upstream 100 bases:

>100_bases
TCACCTCTAGTCCATTTATTATAACATGCAAAATCTACAAAACCTTAACTCTTGTGTTATAATTTCTTATACATGTTAAA
AACATATTTAGGAGCAACGC

Downstream 100 bases:

>100_bases
AAAAAATACGCATATACTGGAGATGTATATGCGTATTATTATTTATATATTTAGGACATTAAATCATGTTATATTAAAAA
ACTATTTTTTTACCGGTTTT

Product: 3-hydroxy-3-methylglutaryl CoA synthetase

Products: (S)-3-hydroxy-3-methylglutaryl-CoA; CoA

Alternate protein names: HMG synthase [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MKIGIHKMSFYVPKFYLDINTLAHARGIDPLKYTNGLLTHKMSVAPFNQDIVTLAANATLNYLTKEDIKAIDLILFTTES
GIDYSKSAATHLISILGLRNDVRALEMKQACYATTAALYFAKGHILQHPESKVLVIASDIAKYGLNSSGEPTQGAGAVSM
IVSREPNIMVLEDQYGIHSEDIYDFWRPDGLDYAQVDGKFSNEMYKKFFLKTFDNYKMQTKHTLDDFAALTFHIPYPKIG
LRSLELVADKANNPSLFENFYTSISYNKYIGNIYTGSLFLSIIALLEQGNLKAGERIGMFGYGSGAVAEFFSAKLVEGYK
AHLNKELHEKQLSNRTELDMEKYAVYLTRKVTNDTVLEDDLDVKVQLKSITDYKRNYQNN

Sequences:

>Translated_380_residues
MKIGIHKMSFYVPKFYLDINTLAHARGIDPLKYTNGLLTHKMSVAPFNQDIVTLAANATLNYLTKEDIKAIDLILFTTES
GIDYSKSAATHLISILGLRNDVRALEMKQACYATTAALYFAKGHILQHPESKVLVIASDIAKYGLNSSGEPTQGAGAVSM
IVSREPNIMVLEDQYGIHSEDIYDFWRPDGLDYAQVDGKFSNEMYKKFFLKTFDNYKMQTKHTLDDFAALTFHIPYPKIG
LRSLELVADKANNPSLFENFYTSISYNKYIGNIYTGSLFLSIIALLEQGNLKAGERIGMFGYGSGAVAEFFSAKLVEGYK
AHLNKELHEKQLSNRTELDMEKYAVYLTRKVTNDTVLEDDLDVKVQLKSITDYKRNYQNN
>Mature_380_residues
MKIGIHKMSFYVPKFYLDINTLAHARGIDPLKYTNGLLTHKMSVAPFNQDIVTLAANATLNYLTKEDIKAIDLILFTTES
GIDYSKSAATHLISILGLRNDVRALEMKQACYATTAALYFAKGHILQHPESKVLVIASDIAKYGLNSSGEPTQGAGAVSM
IVSREPNIMVLEDQYGIHSEDIYDFWRPDGLDYAQVDGKFSNEMYKKFFLKTFDNYKMQTKHTLDDFAALTFHIPYPKIG
LRSLELVADKANNPSLFENFYTSISYNKYIGNIYTGSLFLSIIALLEQGNLKAGERIGMFGYGSGAVAEFFSAKLVEGYK
AHLNKELHEKQLSNRTELDMEKYAVYLTRKVTNDTVLEDDLDVKVQLKSITDYKRNYQNN

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It catalyzes the aldol condensation between the acetyl group attached to the acyl-carrier-protein AcpK (Ac- AcpK) and

COG id: COG3425

COG function: function code I; 3-hydroxy-3-methylglutaryl CoA synthase

Gene ontology:
GO:0003824: 3-hydroxy-3-methylglutaryl CoA synthetase
GO:0004421: 3-hydroxy-3-methylglutaryl CoA synthetase
GO:0008152: 3-hydroxy-3-methylglutaryl CoA synthetase
GO:0008299: 3-hydroxy-3-methylglutaryl CoA synthetase
GO:0016740: 3-hydroxy-3-methylglutaryl CoA synthetase
GO:0016746: 3-hydroxy-3-methylglutaryl CoA synthetase

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA synthase family [H]

Homologues:

Organism=Homo sapiens, GI148298764, Length=375, Percent_Identity=28.2666666666667, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI148298677, Length=375, Percent_Identity=28.2666666666667, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI5031751, Length=375, Percent_Identity=26.6666666666667, Blast_Score=125, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17560126, Length=399, Percent_Identity=24.812030075188, Blast_Score=111, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6323509, Length=384, Percent_Identity=28.125, Blast_Score=110, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24654139, Length=376, Percent_Identity=27.3936170212766, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24654137, Length=376, Percent_Identity=27.3936170212766, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24654133, Length=376, Percent_Identity=27.3936170212766, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24654135, Length=376, Percent_Identity=27.3936170212766, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17933694, Length=376, Percent_Identity=27.3936170212766, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013746
- InterPro:   IPR013528
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF08540 HMG_CoA_synt_C; PF01154 HMG_CoA_synt_N [H]

EC number: 2.3.3.10

Molecular weight: Translated: 42874; Mature: 42874

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGIHKMSFYVPKFYLDINTLAHARGIDPLKYTNGLLTHKMSVAPFNQDIVTLAANATL
CCCCCEEHHEECCEEEEEHHHHHHHCCCCCHHHCCCHHEEEEEECCCCCCEEEEEECCEE
NYLTKEDIKAIDLILFTTESGIDYSKSAATHLISILGLRNDVRALEMKQACYATTAALYF
EHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AKGHILQHPESKVLVIASDIAKYGLNSSGEPTQGAGAVSMIVSREPNIMVLEDQYGIHSE
HCCCEEECCCCCEEEEEEHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCC
DIYDFWRPDGLDYAQVDGKFSNEMYKKFFLKTFDNYKMQTKHTLDDFAALTFHIPYPKIG
HHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCEECHHHHHHHHEEEEEECCCCCCC
LRSLELVADKANNPSLFENFYTSISYNKYIGNIYTGSLFLSIIALLEQGNLKAGERIGMF
CHHEEEECCCCCCCHHHHHHHHHCEECHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEE
GYGSGAVAEFFSAKLVEGYKAHLNKELHEKQLSNRTELDMEKYAVYLTRKVTNDTVLEDD
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEEECCCCCEECCC
LDVKVQLKSITDYKRNYQNN
CCEEEEEEHHHHHHHHCCCC
>Mature Secondary Structure
MKIGIHKMSFYVPKFYLDINTLAHARGIDPLKYTNGLLTHKMSVAPFNQDIVTLAANATL
CCCCCEEHHEECCEEEEEHHHHHHHCCCCCHHHCCCHHEEEEEECCCCCCEEEEEECCEE
NYLTKEDIKAIDLILFTTESGIDYSKSAATHLISILGLRNDVRALEMKQACYATTAALYF
EHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AKGHILQHPESKVLVIASDIAKYGLNSSGEPTQGAGAVSMIVSREPNIMVLEDQYGIHSE
HCCCEEECCCCCEEEEEEHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCC
DIYDFWRPDGLDYAQVDGKFSNEMYKKFFLKTFDNYKMQTKHTLDDFAALTFHIPYPKIG
HHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCEECHHHHHHHHEEEEEECCCCCCC
LRSLELVADKANNPSLFENFYTSISYNKYIGNIYTGSLFLSIIALLEQGNLKAGERIGMF
CHHEEEECCCCCCCHHHHHHHHHCEECHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEE
GYGSGAVAEFFSAKLVEGYKAHLNKELHEKQLSNRTELDMEKYAVYLTRKVTNDTVLEDD
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEEECCCCCEECCC
LDVKVQLKSITDYKRNYQNN
CCEEEEEEHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA; H2O; acetoacetyl-CoA

Specific reaction: acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]