Click here to switch to the map view.

The map label for this gene is mscL [H]

Identifier: 162447083

GI number: 162447083

Start: 205744

End: 206280

Strand: Direct

Name: mscL [H]

Synonym: ACL_0213

Alternate gene names: 162447083

Gene position: 205744-206280 (Clockwise)

Preceding gene: 162447082

Following gene: 162447084

Centisome position: 13.74

GC content: 30.73

Gene sequence:

>537_bases
ATGGGTAAACAAATCAAAATATCCAAGGAACAACGTAAAAAAGGTTTCTTAAATGGATTTAAGAATTTTATCATGCGTGG
AAACGTTATTGATATGGCAGTGGGTGTAATTGTTGGTGGAGCTTTCGGTAAGATTGTTACAAGTCTTGTCAATGACATTA
TATTACCTCCAATTGGTGTTTTATTAGGCGGTGTGGAGTTTAGAGACTTACAAGCACTGATTCATCAAAAACCAGTTTTA
GATGATAATGGTGCACAAGTCGTGATTGATGGTATTGCACAATTTAATAATGTATATATTCGTTATGGGAATTTAATTCA
AATCATTTTAGAATTCTTAATTATTGCATTTTCTATTTATCTCGTTTTATATATCTTTATAAAACGTAAGGAACAAGAAG
AGAAATTTATTGCTGAAGAAAAAGCAAGACTAGAAAAATTAGAAGCTGAAAAAAATCCTCCAAAACCAGCACCAAAACCA
GAAGACATTCAATTGCTAATCGAAATTAGGGATTTACTAAAGAAAAATGAAAAGTAA

Upstream 100 bases:

>100_bases
ATTGCTGGTAAAATTAATGTAGATATAAAATATAAACTATATAGGAAAGGATATATATTATAAAAGAGATATACTCTTTT
ATAATATTGAGAAGATAAGT

Downstream 100 bases:

>100_bases
ATTTAAGGTAATCGTCTGCTCTAACTCTGCAATAGATTATTTAGAGTATGATAAGCAAATTGATATTTTTAGAAGTATGA
TTCACTTTGGGGATGAATCT

Product: large conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 178; Mature: 177

Protein sequence:

>178_residues
MGKQIKISKEQRKKGFLNGFKNFIMRGNVIDMAVGVIVGGAFGKIVTSLVNDIILPPIGVLLGGVEFRDLQALIHQKPVL
DDNGAQVVIDGIAQFNNVYIRYGNLIQIILEFLIIAFSIYLVLYIFIKRKEQEEKFIAEEKARLEKLEAEKNPPKPAPKP
EDIQLLIEIRDLLKKNEK

Sequences:

>Translated_178_residues
MGKQIKISKEQRKKGFLNGFKNFIMRGNVIDMAVGVIVGGAFGKIVTSLVNDIILPPIGVLLGGVEFRDLQALIHQKPVL
DDNGAQVVIDGIAQFNNVYIRYGNLIQIILEFLIIAFSIYLVLYIFIKRKEQEEKFIAEEKARLEKLEAEKNPPKPAPKP
EDIQLLIEIRDLLKKNEK
>Mature_177_residues
GKQIKISKEQRKKGFLNGFKNFIMRGNVIDMAVGVIVGGAFGKIVTSLVNDIILPPIGVLLGGVEFRDLQALIHQKPVLD
DNGAQVVIDGIAQFNNVYIRYGNLIQIILEFLIIAFSIYLVLYIFIKRKEQEEKFIAEEKARLEKLEAEKNPPKPAPKPE
DIQLLIEIRDLLKKNEK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=157, Percent_Identity=38.8535031847134, Blast_Score=104, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 20141; Mature: 20010

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKQIKISKEQRKKGFLNGFKNFIMRGNVIDMAVGVIVGGAFGKIVTSLVNDIILPPIGV
CCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHH
LLGGVEFRDLQALIHQKPVLDDNGAQVVIDGIAQFNNVYIRYGNLIQIILEFLIIAFSIY
HHCCCHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHH
LVLYIFIKRKEQEEKFIAEEKARLEKLEAEKNPPKPAPKPEDIQLLIEIRDLLKKNEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GKQIKISKEQRKKGFLNGFKNFIMRGNVIDMAVGVIVGGAFGKIVTSLVNDIILPPIGV
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHH
LLGGVEFRDLQALIHQKPVLDDNGAQVVIDGIAQFNNVYIRYGNLIQIILEFLIIAFSIY
HHCCCHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHH
LVLYIFIKRKEQEEKFIAEEKARLEKLEAEKNPPKPAPKPEDIQLLIEIRDLLKKNEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA