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The map label for this gene is rlmH

Identifier: 162447082

GI number: 162447082

Start: 205145

End: 205588

Strand: Direct

Name: rlmH

Synonym: ACL_0212

Alternate gene names: 162447082

Gene position: 205145-205588 (Clockwise)

Preceding gene: 162447081

Following gene: 162447083

Centisome position: 13.7

GC content: 30.41

Gene sequence:

>444_bases
ATGAAAGTAAAGATTATTAGTGTTGGTAAAGTTAAAGATAAGGGATTAAATGAATTAATCAATTACTACGCAAAACAAAT
AAAAACCATTGAAATGGTTGAAATAACAGATGAAGCAAGTATCACAGGTATGGAAAAAGAAGGACAAAAAATATTGTCTA
AAATCGCAAAAGATAGTTATGTCATATCACTAGCTATTGAAGGTAAGATGTTAGATAGTGTTGAGTTTGCAGACACCTTA
GATCATGTCACAACAAACTTTGGTCCGTCAATTACCTTCATCATTGGTGGCTCATTTGGTTTAAGTCATGCTGTTAAAGA
AAAGTCAAATCTGCTTTTATCTTTTTCAAAGATGACTTTCCCACACCAGTTAATGAAACTATTTTTAGTAGAACAAATTT
TTAGAGCACAAGCTATTTTAAACAATCACCCTTATCATAAATAA

Upstream 100 bases:

>100_bases
ATGCCTTTGACATTGAAAAAGCAATTGTAGATCATGTAGAAGACTTACTTAAGATTGATGTGATCTTTGCATCACAAGAA
TCCTTACCGGGTATTAAACT

Downstream 100 bases:

>100_bases
AGGTGATTGTTTTTATTAAGATAGATTCTAATGATTAATCAAGACTTTTAGTGTAATTGCTGGTAAAATTAATGTAGATA
TAAAATATAAACTATATAGG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MKVKIISVGKVKDKGLNELINYYAKQIKTIEMVEITDEASITGMEKEGQKILSKIAKDSYVISLAIEGKMLDSVEFADTL
DHVTTNFGPSITFIIGGSFGLSHAVKEKSNLLLSFSKMTFPHQLMKLFLVEQIFRAQAILNNHPYHK

Sequences:

>Translated_147_residues
MKVKIISVGKVKDKGLNELINYYAKQIKTIEMVEITDEASITGMEKEGQKILSKIAKDSYVISLAIEGKMLDSVEFADTL
DHVTTNFGPSITFIIGGSFGLSHAVKEKSNLLLSFSKMTFPHQLMKLFLVEQIFRAQAILNNHPYHK
>Mature_147_residues
MKVKIISVGKVKDKGLNELINYYAKQIKTIEMVEITDEASITGMEKEGQKILSKIAKDSYVISLAIEGKMLDSVEFADTL
DHVTTNFGPSITFIIGGSFGLSHAVKEKSNLLLSFSKMTFPHQLMKLFLVEQIFRAQAILNNHPYHK

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=159, Percent_Identity=31.4465408805031, Blast_Score=86, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_ACHLI (A9NEQ8)

Other databases:

- EMBL:   CP000896
- RefSeq:   YP_001620214.1
- ProteinModelPortal:   A9NEQ8
- SMR:   A9NEQ8
- GeneID:   5804219
- GenomeReviews:   CP000896_GR
- KEGG:   acl:ACL_0212
- HOGENOM:   HBG622807
- OMA:   NISHYKI
- BioCyc:   ALAI441768:ACL_0212-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR003742
- PIRSF:   PIRSF004505

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 16478; Mature: 16478

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKIISVGKVKDKGLNELINYYAKQIKTIEMVEITDEASITGMEKEGQKILSKIAKDSY
CEEEEEEECCCCCCCHHHHHHHHHHHHCHHEEEEECCCCCCCCCHHHHHHHHHHHCCCCE
VISLAIEGKMLDSVEFADTLDHVTTNFGPSITFIIGGSFGLSHAVKEKSNLLLSFSKMTF
EEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHEEEHHHCCC
PHQLMKLFLVEQIFRAQAILNNHPYHK
HHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKVKIISVGKVKDKGLNELINYYAKQIKTIEMVEITDEASITGMEKEGQKILSKIAKDSY
CEEEEEEECCCCCCCHHHHHHHHHHHHCHHEEEEECCCCCCCCCHHHHHHHHHHHCCCCE
VISLAIEGKMLDSVEFADTLDHVTTNFGPSITFIIGGSFGLSHAVKEKSNLLLSFSKMTF
EEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHEEEHHHCCC
PHQLMKLFLVEQIFRAQAILNNHPYHK
HHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA