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The map label for this gene is prfA

Identifier: 162447071

GI number: 162447071

Start: 192438

End: 193502

Strand: Direct

Name: prfA

Synonym: ACL_0201

Alternate gene names: 162447071

Gene position: 192438-193502 (Clockwise)

Preceding gene: 162447070

Following gene: 162447072

Centisome position: 12.85

GC content: 34.55

Gene sequence:

>1065_bases
ATGTTTGAAAGATTAGAAGTCATGAGAAAAACGTATTATGCGTTACAAGAAAAATTGGCATCAGGTATTAGTGATGTCAA
AGAGATTACAAAACTCATGAAAGAACTTAAAAGTTTAGAAGATGCTGTCGTAGCATATGAAAAATATTTAAGTTTAAAAG
AACAATTAAAAGATTTAGAAGAACTACTTGAGTTAGAAACTGAAAGTAGTGTTTTAGAAATGGCGAAAGCTGAAGAAAAA
TCACTAGAAAGTGATATTGAAAACTTAGAAGAAAGTTTACGTATTTTACTTCTACCTAAAGATCCAGATGACGATAAAAA
CGTCATCATTGAAATTAAGGGTGCAGCAGGTGGAGATGAAGGTAACATCTTTGCAGGTGATTTATTTAAAATGTATTCTA
AATATGCTGAAAGTATGGGCTGGAAAGTTACACTTGTAAATACAACACCAGGTTCTAGTGGTGGTTTTGCAGGTATAGAG
TTCATCATTTCTGGTGAGAATGCATTTAGTTATTTAAAACACGAATCCGGTGTACACCGCGTACAACGCGTACCTGAAAC
AGAAAGCCAAGGGCGCATTCATACTTCAACTGCAGTCGTACTTGCATTACCTGAACAAGAGGATATTGAGTATGATGTCA
AGTGGGAAGATATCCGTTTTGATACATATAACTCATCAGGTGCAGGTGGACAATCTGTTAATACAACTTATTCAGCAGTT
CGTTTAACGCATATACCTACAAATGTCGTTGTAACATCACAAGAAGAACGTAGTCAACATGCAAATAAGGATAGAGCTTA
TAAATTATTAGTCACTCGTATTTATGATAAAATTCAACAAGAGCAACTAGAAAAAGAAGGCGAAAGTAGAAAAGCTTTAA
TCGGACGTGGTAACCGTAGTGAAAAGATTAGAACCTATAACTACCCACAAAACCGAGTAACAGATCACCGTATTGGTTTA
ACCATTAATAGACTAGATGCAATTATGGAAGGTCGTATTGATTTAATTATCGAACCACTCATTAACGAAATCCAAAAAGA
AGCATTAGAAGGACAATCAAAGTGA

Upstream 100 bases:

>100_bases
GTTGTGTCATTTGTATTTAAAAAGATAAATCCTATATGGCTGATCATACTATCGGGTATACTTGGATTTATTGGTTATAC
TATTTTCTAAGGAGAAAATT

Downstream 100 bases:

>100_bases
CACTTCAAGACTTAGTAAAAACCTATGAAAAAAGGGTTATCAGTTTAGGTAAAGAACCACATGCAATGAGTTGGTTAATT
ACCGAATTATCAGGTTATTC

Product: peptide chain release factor RF-1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MFERLEVMRKTYYALQEKLASGISDVKEITKLMKELKSLEDAVVAYEKYLSLKEQLKDLEELLELETESSVLEMAKAEEK
SLESDIENLEESLRILLLPKDPDDDKNVIIEIKGAAGGDEGNIFAGDLFKMYSKYAESMGWKVTLVNTTPGSSGGFAGIE
FIISGENAFSYLKHESGVHRVQRVPETESQGRIHTSTAVVLALPEQEDIEYDVKWEDIRFDTYNSSGAGGQSVNTTYSAV
RLTHIPTNVVVTSQEERSQHANKDRAYKLLVTRIYDKIQQEQLEKEGESRKALIGRGNRSEKIRTYNYPQNRVTDHRIGL
TINRLDAIMEGRIDLIIEPLINEIQKEALEGQSK

Sequences:

>Translated_354_residues
MFERLEVMRKTYYALQEKLASGISDVKEITKLMKELKSLEDAVVAYEKYLSLKEQLKDLEELLELETESSVLEMAKAEEK
SLESDIENLEESLRILLLPKDPDDDKNVIIEIKGAAGGDEGNIFAGDLFKMYSKYAESMGWKVTLVNTTPGSSGGFAGIE
FIISGENAFSYLKHESGVHRVQRVPETESQGRIHTSTAVVLALPEQEDIEYDVKWEDIRFDTYNSSGAGGQSVNTTYSAV
RLTHIPTNVVVTSQEERSQHANKDRAYKLLVTRIYDKIQQEQLEKEGESRKALIGRGNRSEKIRTYNYPQNRVTDHRIGL
TINRLDAIMEGRIDLIIEPLINEIQKEALEGQSK
>Mature_354_residues
MFERLEVMRKTYYALQEKLASGISDVKEITKLMKELKSLEDAVVAYEKYLSLKEQLKDLEELLELETESSVLEMAKAEEK
SLESDIENLEESLRILLLPKDPDDDKNVIIEIKGAAGGDEGNIFAGDLFKMYSKYAESMGWKVTLVNTTPGSSGGFAGIE
FIISGENAFSYLKHESGVHRVQRVPETESQGRIHTSTAVVLALPEQEDIEYDVKWEDIRFDTYNSSGAGGQSVNTTYSAV
RLTHIPTNVVVTSQEERSQHANKDRAYKLLVTRIYDKIQQEQLEKEGESRKALIGRGNRSEKIRTYNYPQNRVTDHRIGL
TINRLDAIMEGRIDLIIEPLINEIQKEALEGQSK

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:
GO:0003747: Peptide chain release factor 1
GO:0005737: Peptide chain release factor 1
GO:0006412: Peptide chain release factor 1
GO:0006415: Peptide chain release factor 1
GO:0016149: Peptide chain release factor 1

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=314, Percent_Identity=40.7643312101911, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI34577120, Length=303, Percent_Identity=36.963696369637, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI166795305, Length=214, Percent_Identity=39.2523364485981, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI1787462, Length=349, Percent_Identity=44.9856733524355, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI2367172, Length=313, Percent_Identity=36.4217252396166, Blast_Score=179, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17542784, Length=282, Percent_Identity=37.9432624113475, Blast_Score=170, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6321295, Length=337, Percent_Identity=41.2462908011869, Blast_Score=213, Evalue=3e-56,
Organism=Drosophila melanogaster, GI19921226, Length=357, Percent_Identity=37.2549019607843, Blast_Score=213, Evalue=2e-55,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_ACHLI (A9NEP7)

Other databases:

- EMBL:   CP000896
- RefSeq:   YP_001620203.1
- ProteinModelPortal:   A9NEP7
- SMR:   A9NEP7
- GeneID:   5804200
- GenomeReviews:   CP000896_GR
- KEGG:   acl:ACL_0201
- HOGENOM:   HBG629764
- OMA:   NTNEGEH
- BioCyc:   ALAI441768:ACL_0201-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40086; Mature: 40086

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFERLEVMRKTYYALQEKLASGISDVKEITKLMKELKSLEDAVVAYEKYLSLKEQLKDLE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELLELETESSVLEMAKAEEKSLESDIENLEESLRILLLPKDPDDDKNVIIEIKGAAGGDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEECCCCCCC
GNIFAGDLFKMYSKYAESMGWKVTLVNTTPGSSGGFAGIEFIISGENAFSYLKHESGVHR
CCEEHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHH
VQRVPETESQGRIHTSTAVVLALPEQEDIEYDVKWEDIRFDTYNSSGAGGQSVNTTYSAV
HHHCCCCCCCCEEEECEEEEEECCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCHHHEE
RLTHIPTNVVVTSQEERSQHANKDRAYKLLVTRIYDKIQQEQLEKEGESRKALIGRGNRS
EEEECCCCEEEECHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCC
EKIRTYNYPQNRVTDHRIGLTINRLDAIMEGRIDLIIEPLINEIQKEALEGQSK
CCCEEECCCCCCCCCHHHCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFERLEVMRKTYYALQEKLASGISDVKEITKLMKELKSLEDAVVAYEKYLSLKEQLKDLE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELLELETESSVLEMAKAEEKSLESDIENLEESLRILLLPKDPDDDKNVIIEIKGAAGGDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEECCCCCCC
GNIFAGDLFKMYSKYAESMGWKVTLVNTTPGSSGGFAGIEFIISGENAFSYLKHESGVHR
CCEEHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHH
VQRVPETESQGRIHTSTAVVLALPEQEDIEYDVKWEDIRFDTYNSSGAGGQSVNTTYSAV
HHHCCCCCCCCEEEECEEEEEECCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCHHHEE
RLTHIPTNVVVTSQEERSQHANKDRAYKLLVTRIYDKIQQEQLEKEGESRKALIGRGNRS
EEEECCCCEEEECHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCC
EKIRTYNYPQNRVTDHRIGLTINRLDAIMEGRIDLIIEPLINEIQKEALEGQSK
CCCEEECCCCCCCCCHHHCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA