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The map label for this gene is ywkE [H]

Identifier: 162447072

GI number: 162447072

Start: 193499

End: 194350

Strand: Direct

Name: ywkE [H]

Synonym: ACL_0202

Alternate gene names: 162447072

Gene position: 193499-194350 (Clockwise)

Preceding gene: 162447071

Following gene: 162447073

Centisome position: 12.93

GC content: 33.1

Gene sequence:

>852_bases
GTGACACTTCAAGACTTAGTAAAAACCTATGAAAAAAGGGTTATCAGTTTAGGTAAAGAACCACATGCAATGAGTTGGTT
AATTACCGAATTATCAGGTTATTCACCAACAGAGTTTTATTTAAAGATGAATTCTAAAGTAAGTGAAGAACTCATTAACA
AAATCGAAGATGGTTTTAAACGCTACGCCATAGAAGGTACCCCTGTACAACATATTGTAGGTTACTCTTACTTTTATGGT
TACAAATTTAAAGTCAGTGATCAAGTATTAATACCAAGAAGAGAAACAGAACAATTAGTGGAAGAAACACTACTTATCTA
TGACACATATTTTGAAGACCAAAAAGTCGATGTACTTGATTTAGGTACCGGTAGTGGAGCGATTGCTATTACATTATCTA
TTGAAGAACCTAACATGCAAGTAGAGGCATCAGATATCTCTGTGACAGCACTTATGGTTGCAAGAGAAAACCAATTAAAC
TTAAGCAGCAAAGTGAATTTTATTGCATCGGATTGGTTTAGTAACATCAACAAAAAATACGATATAATCGTCGCAAATCC
GCCTTACATTCCAAATGATGAAGCAGTAGAGGATGTTGTAACAAAAGAACCTAGTTTAGCCCTTTACGGCGGTGTAGATG
GACTTGAACCTTATGAGATTATCTTAAAGAATGCTAAAAACTATCTAAAAGAAAAGGCACTCATTGCCTTTGAACATAGT
ATGTATCAAAGTGAAAATTTAAAAACTTTAATCTTAAAATATTTTAAAGATGCTAAAGTAAGACAACTAAAAGACTTACA
AGGTAAAGATCGTATGACGTTTGTTGGTTTAGGTGGTGTATTTAATGACTAA

Upstream 100 bases:

>100_bases
CCATTAATAGACTAGATGCAATTATGGAAGGTCGTATTGATTTAATTATCGAACCACTCATTAACGAAATCCAAAAAGAA
GCATTAGAAGGACAATCAAA

Downstream 100 bases:

>100_bases
ATTACTTATTTTCCCAACTGATACTGTTTATGGATTAGGCACACCTTATTTAGATAAGGAAGGCTTAAAACAAATCTATG
AAATTAAAGGTAGAGACTTT

Product: HemK family SAM-dependent methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTLQDLVKTYEKRVISLGKEPHAMSWLITELSGYSPTEFYLKMNSKVSEELINKIEDGFKRYAIEGTPVQHIVGYSYFYG
YKFKVSDQVLIPRRETEQLVEETLLIYDTYFEDQKVDVLDLGTGSGAIAITLSIEEPNMQVEASDISVTALMVARENQLN
LSSKVNFIASDWFSNINKKYDIIVANPPYIPNDEAVEDVVTKEPSLALYGGVDGLEPYEIILKNAKNYLKEKALIAFEHS
MYQSENLKTLILKYFKDAKVRQLKDLQGKDRMTFVGLGGVFND

Sequences:

>Translated_283_residues
MTLQDLVKTYEKRVISLGKEPHAMSWLITELSGYSPTEFYLKMNSKVSEELINKIEDGFKRYAIEGTPVQHIVGYSYFYG
YKFKVSDQVLIPRRETEQLVEETLLIYDTYFEDQKVDVLDLGTGSGAIAITLSIEEPNMQVEASDISVTALMVARENQLN
LSSKVNFIASDWFSNINKKYDIIVANPPYIPNDEAVEDVVTKEPSLALYGGVDGLEPYEIILKNAKNYLKEKALIAFEHS
MYQSENLKTLILKYFKDAKVRQLKDLQGKDRMTFVGLGGVFND
>Mature_282_residues
TLQDLVKTYEKRVISLGKEPHAMSWLITELSGYSPTEFYLKMNSKVSEELINKIEDGFKRYAIEGTPVQHIVGYSYFYGY
KFKVSDQVLIPRRETEQLVEETLLIYDTYFEDQKVDVLDLGTGSGAIAITLSIEEPNMQVEASDISVTALMVARENQLNL
SSKVNFIASDWFSNINKKYDIIVANPPYIPNDEAVEDVVTKEPSLALYGGVDGLEPYEIILKNAKNYLKEKALIAFEHSM
YQSENLKTLILKYFKDAKVRQLKDLQGKDRMTFVGLGGVFND

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=235, Percent_Identity=28.936170212766, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787463, Length=253, Percent_Identity=29.6442687747036, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI87082085, Length=192, Percent_Identity=34.8958333333333, Blast_Score=102, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24582226, Length=200, Percent_Identity=31.5, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 32205; Mature: 32074

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQDLVKTYEKRVISLGKEPHAMSWLITELSGYSPTEFYLKMNSKVSEELINKIEDGFK
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHH
RYAIEGTPVQHIVGYSYFYGYKFKVSDQVLIPRRETEQLVEETLLIYDTYFEDQKVDVLD
HEECCCCCHHHHHHHHHHEEEEEEECCEEECCCHHHHHHHHHHHEEEEECCCCCCEEEEE
LGTGSGAIAITLSIEEPNMQVEASDISVTALMVARENQLNLSSKVNFIASDWFSNINKKY
ECCCCCEEEEEEEEECCCCEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEE
DIIVANPPYIPNDEAVEDVVTKEPSLALYGGVDGLEPYEIILKNAKNYLKEKALIAFEHS
EEEEECCCCCCCCHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEEHHH
MYQSENLKTLILKYFKDAKVRQLKDLQGKDRMTFVGLGGVFND
HHCCCCHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
TLQDLVKTYEKRVISLGKEPHAMSWLITELSGYSPTEFYLKMNSKVSEELINKIEDGFK
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHH
RYAIEGTPVQHIVGYSYFYGYKFKVSDQVLIPRRETEQLVEETLLIYDTYFEDQKVDVLD
HEECCCCCHHHHHHHHHHEEEEEEECCEEECCCHHHHHHHHHHHEEEEECCCCCCEEEEE
LGTGSGAIAITLSIEEPNMQVEASDISVTALMVARENQLNLSSKVNFIASDWFSNINKKY
ECCCCCEEEEEEEEECCCCEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEE
DIIVANPPYIPNDEAVEDVVTKEPSLALYGGVDGLEPYEIILKNAKNYLKEKALIAFEHS
EEEEECCCCCCCCHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEEHHH
MYQSENLKTLILKYFKDAKVRQLKDLQGKDRMTFVGLGGVFND
HHCCCCHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]