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The map label for this gene is tuf

Identifier: 162447058

GI number: 162447058

Start: 181291

End: 182478

Strand: Direct

Name: tuf

Synonym: ACL_0188

Alternate gene names: 162447058

Gene position: 181291-182478 (Clockwise)

Preceding gene: 162447057

Following gene: 162447059

Centisome position: 12.11

GC content: 38.97

Gene sequence:

>1188_bases
ATGGCAAAACAAAAATTCGAAAGAACAAAACCACACGTTAACGTTGGAACAATCGGACACGTTGACCATGGTAAAACTAC
ATTAACTGCTGCAATTACATCAGTACTTGCAGGAAAAGGTTTAGCTACAAAAAGAGATTATAACCAAATCGATGGTGCAC
CAGAAGAAAAAGCTCGTGGTATAACTATTAACGCATCTCACGTTGAGTATGAAACTGTAAACAGACACTACGCTCACGTA
GACTGCCCAGGTCACGCTGACTATGTTAAAAACATGATTACAGGTGCTGCTCAAATGGACGGTGCTATCTTAGTTGTTTC
TGCAGCGGATGGTCCAATGCCACAAACTCGTGAACATATTCTTTTATCACGCCAAGTTGGGGTACCTAAATTAGTAGTAT
TCTTAAACAAAGCTGACTTAGTTGATGACGAAGAGTTATTAGACTTAGTTGAAATGGAAGTTAGAGAATTATTATCAGAA
TATGATTTCCCAGGAGATGATATTCCAGTAATTAAAGGATCTGCTTTAGGCGCTTTAGAAGGTAAACCAGAATGGGTTGC
TAAAGTTGAAGAATTAATGGATGCAGTTGATGCATACATCGATACTCCATTACGTGCTACTGACAAACCATTTATGATGC
CAGTTGAAGACGTATTCACAATTACAGGTCGTGGTACAGTTGCTACAGGACGTGTTGATCGTGGTATCGTTAAAGTTGGT
GACCAAGTTGAAATCGTTGGTATTACTGATACTAAAACTACAACTGTTACAGGTGTAGAAATGTTTAGAAAATTATTAGA
CCAAGCTGAAGCTGGAGACAACATCGGTGCTTTATTAAGAGGTGTTGATCGTGAAGGTGTAGAACGTGGTCAAGTATTAT
CAAAACCAGGTACAGTTAAACCTCATGCTAAATTCACTGCTCAAATTTATGTATTAAGTAAAGAAGAAGGTGGACGCCAT
ACAGCGTTCTTCTCAAACTATCGTCCTCAATTCTACTTCAGAACTACAGACATCACAGGTATCATCACTTTAGGTGAAGG
TACTGAAATGGTTATGCCAGGTGATAACGCTGAAGTTACAGTAGAACTTATTCACCCAATCGCGCTTGAAGAAGGTACAA
AATTCTCAATCCGCGAAGGTGGTAGAACAGTTGCTTCTGGATCAGTAGTTAAAATTCTTGCTGACTAA

Upstream 100 bases:

>100_bases
GTGGTTCAAACTAATCCACTTCACAAAAAATTAGTAATATGATAAAATATCTATTGATAGGCTATTGTATTAATTAAAAA
TTATAGGAGGCTTTTGCGTT

Downstream 100 bases:

>100_bases
TTAAAGTTTAAAAACTTATGGAACCTATCCTGTTGGATAGGTTCTTTTTTATATACAATATATGTAATTCTTACGAGATT
CATACCACAAAACCCCATCT

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MAKQKFERTKPHVNVGTIGHVDHGKTTLTAAITSVLAGKGLATKRDYNQIDGAPEEKARGITINASHVEYETVNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPKLVVFLNKADLVDDEELLDLVEMEVRELLSE
YDFPGDDIPVIKGSALGALEGKPEWVAKVEELMDAVDAYIDTPLRATDKPFMMPVEDVFTITGRGTVATGRVDRGIVKVG
DQVEIVGITDTKTTTVTGVEMFRKLLDQAEAGDNIGALLRGVDREGVERGQVLSKPGTVKPHAKFTAQIYVLSKEEGGRH
TAFFSNYRPQFYFRTTDITGIITLGEGTEMVMPGDNAEVTVELIHPIALEEGTKFSIREGGRTVASGSVVKILAD

Sequences:

>Translated_395_residues
MAKQKFERTKPHVNVGTIGHVDHGKTTLTAAITSVLAGKGLATKRDYNQIDGAPEEKARGITINASHVEYETVNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPKLVVFLNKADLVDDEELLDLVEMEVRELLSE
YDFPGDDIPVIKGSALGALEGKPEWVAKVEELMDAVDAYIDTPLRATDKPFMMPVEDVFTITGRGTVATGRVDRGIVKVG
DQVEIVGITDTKTTTVTGVEMFRKLLDQAEAGDNIGALLRGVDREGVERGQVLSKPGTVKPHAKFTAQIYVLSKEEGGRH
TAFFSNYRPQFYFRTTDITGIITLGEGTEMVMPGDNAEVTVELIHPIALEEGTKFSIREGGRTVASGSVVKILAD
>Mature_394_residues
AKQKFERTKPHVNVGTIGHVDHGKTTLTAAITSVLAGKGLATKRDYNQIDGAPEEKARGITINASHVEYETVNRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPKLVVFLNKADLVDDEELLDLVEMEVRELLSEY
DFPGDDIPVIKGSALGALEGKPEWVAKVEELMDAVDAYIDTPLRATDKPFMMPVEDVFTITGRGTVATGRVDRGIVKVGD
QVEIVGITDTKTTTVTGVEMFRKLLDQAEAGDNIGALLRGVDREGVERGQVLSKPGTVKPHAKFTAQIYVLSKEEGGRHT
AFFSNYRPQFYFRTTDITGIITLGEGTEMVMPGDNAEVTVELIHPIALEEGTKFSIREGGRTVASGSVVKILAD

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=57.7215189873418, Blast_Score=475, Evalue=1e-134,
Organism=Homo sapiens, GI223555963, Length=433, Percent_Identity=29.7921478060046, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI5729864, Length=433, Percent_Identity=29.7921478060046, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI4503475, Length=353, Percent_Identity=32.2946175637394, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=30.1339285714286, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI54607086, Length=311, Percent_Identity=28.9389067524116, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI194018522, Length=446, Percent_Identity=26.457399103139, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI194097354, Length=446, Percent_Identity=26.457399103139, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI194018520, Length=446, Percent_Identity=26.457399103139, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI46094014, Length=456, Percent_Identity=26.5350877192982, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=35.8208955223881, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI157426893, Length=235, Percent_Identity=28.0851063829787, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=72.8426395939086, Blast_Score=584, Evalue=1e-168,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=72.8426395939086, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI2367247, Length=387, Percent_Identity=32.0413436692506, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI48994988, Length=272, Percent_Identity=32.3529411764706, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789108, Length=316, Percent_Identity=29.746835443038, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=53.6340852130326, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI25141371, Length=382, Percent_Identity=46.3350785340314, Blast_Score=339, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI17552884, Length=449, Percent_Identity=30.5122494432071, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17569207, Length=449, Percent_Identity=30.5122494432071, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI32566303, Length=431, Percent_Identity=29.4663573085847, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI115532067, Length=435, Percent_Identity=27.1264367816092, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=435, Percent_Identity=27.1264367816092, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=452, Percent_Identity=25.8849557522124, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17506081, Length=276, Percent_Identity=27.536231884058, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI32566301, Length=166, Percent_Identity=32.5301204819277, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=283, Percent_Identity=27.208480565371, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=239, Percent_Identity=25.1046025104602, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=395, Percent_Identity=63.2911392405063, Blast_Score=515, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6325337, Length=349, Percent_Identity=32.9512893982808, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6319594, Length=349, Percent_Identity=32.9512893982808, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6320377, Length=339, Percent_Identity=28.023598820059, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6322937, Length=352, Percent_Identity=24.4318181818182, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320863, Length=276, Percent_Identity=26.4492753623188, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=242, Percent_Identity=30.1652892561983, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=57.7608142493639, Blast_Score=473, Evalue=1e-134,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=57.7608142493639, Blast_Score=473, Evalue=1e-134,
Organism=Drosophila melanogaster, GI19921738, Length=388, Percent_Identity=51.0309278350515, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI45553807, Length=449, Percent_Identity=30.5122494432071, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI45553816, Length=449, Percent_Identity=30.5122494432071, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24651721, Length=449, Percent_Identity=30.5122494432071, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI17864154, Length=449, Percent_Identity=30.5122494432071, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=30.7349665924276, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=30.7349665924276, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=30.3630363036304, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI45550900, Length=351, Percent_Identity=30.1994301994302, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17137380, Length=339, Percent_Identity=30.0884955752212, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24647046, Length=286, Percent_Identity=25.8741258741259, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24647048, Length=286, Percent_Identity=25.8741258741259, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_ACHLI (A9NEN4)

Other databases:

- EMBL:   CP000896
- RefSeq:   YP_001620190.1
- ProteinModelPortal:   A9NEN4
- SMR:   A9NEN4
- GeneID:   5804514
- GenomeReviews:   CP000896_GR
- KEGG:   acl:ACL_0188
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- ProtClustDB:   PRK00049
- BioCyc:   ALAI441768:ACL_0188-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 42851; Mature: 42720

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQKFERTKPHVNVGTIGHVDHGKTTLTAAITSVLAGKGLATKRDYNQIDGAPEEKARG
CCCCHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCHHCCC
ITINASHVEYETVNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEECCCEEEEECCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPKLVVFLNKADLVDDEELLDLVEMEVRELLSEYDFPGDDIPVIKGSALGALE
EEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCC
GKPEWVAKVEELMDAVDAYIDTPLRATDKPFMMPVEDVFTITGRGTVATGRVDRGIVKVG
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEEECCCEEEECCCCCCEEECC
DQVEIVGITDTKTTTVTGVEMFRKLLDQAEAGDNIGALLRGVDREGVERGQVLSKPGTVK
CEEEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHCCCCEECCCCCCC
PHAKFTAQIYVLSKEEGGRHTAFFSNYRPQFYFRTTDITGIITLGEGTEMVMPGDNAEVT
CCCEEEEEEEEEECCCCCCEEEEECCCCCEEEEEEECCEEEEEECCCCEEEECCCCCEEE
VELIHPIALEEGTKFSIREGGRTVASGSVVKILAD
EEEECCEECCCCCEEEECCCCCEEECCCEEEEEEC
>Mature Secondary Structure 
AKQKFERTKPHVNVGTIGHVDHGKTTLTAAITSVLAGKGLATKRDYNQIDGAPEEKARG
CCCHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCHHCCC
ITINASHVEYETVNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEECCCEEEEECCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPKLVVFLNKADLVDDEELLDLVEMEVRELLSEYDFPGDDIPVIKGSALGALE
EEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCC
GKPEWVAKVEELMDAVDAYIDTPLRATDKPFMMPVEDVFTITGRGTVATGRVDRGIVKVG
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEEECCCEEEECCCCCCEEECC
DQVEIVGITDTKTTTVTGVEMFRKLLDQAEAGDNIGALLRGVDREGVERGQVLSKPGTVK
CEEEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHCCCCEECCCCCCC
PHAKFTAQIYVLSKEEGGRHTAFFSNYRPQFYFRTTDITGIITLGEGTEMVMPGDNAEVT
CCCEEEEEEEEEECCCCCCEEEEECCCCCEEEEEEECCEEEEEECCCCEEEECCCCCEEE
VELIHPIALEEGTKFSIREGGRTVASGSVVKILAD
EEEECCEECCCCCEEEECCCCCEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA