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The map label for this gene is gidA

Identifier: 162446948

GI number: 162446948

Start: 70225

End: 72078

Strand: Direct

Name: gidA

Synonym: ACL_0063

Alternate gene names: 162446948

Gene position: 70225-72078 (Clockwise)

Preceding gene: 162446947

Following gene: 162446949

Centisome position: 4.69

GC content: 34.3

Gene sequence:

>1854_bases
ATGATTTATGATGGCATTATTGTAGGTGCTGGACACGCCGGTGTCGAAGCTGCACTTGCTATGGCAAAAATGAAACTTAA
TACTTTACTCATTACAGGCGATCTAACAAAGGTTGCTACTTTGCCATGTAATCCATCAATTGGTGGACCTGCTAAAGGTA
TTGTAGTTAGAGAAATTGACGCACTTGGCGGTCAAATGGCAAAGTCAGCAGACTTAAGTCAAATTCAAATGAAGATGTTA
AATCCTTCAAAAGGTCCAGCAGTTCGTGCACTACGCGCTCAAATTGATAAATTAAAATATCCTAAAGTGATGTTAGATGT
GTTACAAAACACACCGAATCTAACTTTACTTCAAGGATTTGTTGACACATTAATTGTTGAAGACAATACCGTTAAAGGTG
TAAACCTAGAAGATGGTAGTCAAGTATTTGCCCAAACTGTAATTATTACAACAGGTACATATTTAGGTTCTCAAATTTTA
ATTGGTCACGAAAAAACATCAAGTGGTCCAAATGGTGAACGTACAACACGTGGTATTTCTAATCAACTTAAAAACTTAGG
TTTTGAAATGCTTAGACTAAAAACGGGTACACCACCAAGAATTGCTAGAGATTCTATCGACTATTCTAAAACTGTACCAC
AACCAGGTGATGGTGTTTTCCAAACATTTTCACATGATTCACCAATCACTGACTTAGGTCACCAAGAATTTAGCTACTTA
ATCCACACATCACCAGATACTAAAGATATTATATTTAATAACTTAGATGCATCAGCAATGTATGGTGGTGTTGTAGAAGG
TGTTGGTCCAAGATACTGTCCATCTATTGAAGACAAATTTGTTAGATTTAAAGATAAAGACAGACATCAAATCTTTATTG
AACCAGAATCCATGGATTTAAATGAAATGTATATCCAAGGATTATCCACATCGATGCCAAAACATATTCAAGAGCAAATG
GTAAGATCTTTACCTGGTATGGAAAACGCACGTATTGTGCGTTATGCATACGCAATCGAATATGATGCAATTAATCCAAG
ACAACTCTATCAATCCTTAGAGACTAAAGTCATCCATAATCTATTTTGTGCTGGTCAAATTAACGGTACATCAGGTTATG
AAGAAGCTGCTGGTCAAGGTTTAATGGCAGGTATTAATGCAGGATTAAAAGTACAGGGTAAAAAACCACTCGTATTAAAA
AGAGATGAAGCATATATTGGTGTATTAATTGACGACCTTATTACTAAGGGAACTAGTGAACCATATAGACTTTTAACCTC
ACGTGCAGAACATAGACTGTTATTAAGAAACGATAATGCCGATATAAGATTAAGAGATTATGGCTATCAAATCGGTTTAG
TCGATGATGCAACGTATGAAAGATTCGAACATAAGAAAATAGCATTAAAAGAAATGATTGAAAGAGCAAAATCAACAAAG
ATTAACCCAACTGAGGAAAATCTAAACTATTTAGCTTCGGTAAATTCAAGCCCGATTTATGAAGGCGTTACAGTCTTTAA
ATTACTTGAAAGACCTGAACTTAAGATAGAAACCCTTAAACATTTTTTACCATCAGACTATAATCATGAAATCTATGAAC
AATTAGAAATATATATTAAGTATGATGGATATATTGAAAAAGCTAGAAGAGAAGCGGATAAATTATTACGCTATGAATCT
AGATTTATTCCAAACGATATTAATTATCATAGTATTCACAACATATCTGCAGAAGCTAAAGAAAAATTAAGTAAGATTAA
ACCAGAAACATTAGGTCAAGCAACTAGAATCCTAGGTGTTGGACCAACGGATGTATCCATGTTACTCGTTTATCTAGAAG
CCAAGAATGCTTAA

Upstream 100 bases:

>100_bases
TGCAAATGCTGATGTGGTATTAAAACATTTCGGCTTTAACAAAGAAAGCATTGCAGAAGCATTTATAAATATTAAATAAA
AGTTTTAAAGGAAGATATAT

Downstream 100 bases:

>100_bases
TACATACATTACAAATATATTAGGCATCAAGTTAACCGAACAACAAAACGGGCAATTTGATGCCTATTATAACTTATTAG
TAGCATATAATAAGCATACG

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 617; Mature: 617

Protein sequence:

>617_residues
MIYDGIIVGAGHAGVEAALAMAKMKLNTLLITGDLTKVATLPCNPSIGGPAKGIVVREIDALGGQMAKSADLSQIQMKML
NPSKGPAVRALRAQIDKLKYPKVMLDVLQNTPNLTLLQGFVDTLIVEDNTVKGVNLEDGSQVFAQTVIITTGTYLGSQIL
IGHEKTSSGPNGERTTRGISNQLKNLGFEMLRLKTGTPPRIARDSIDYSKTVPQPGDGVFQTFSHDSPITDLGHQEFSYL
IHTSPDTKDIIFNNLDASAMYGGVVEGVGPRYCPSIEDKFVRFKDKDRHQIFIEPESMDLNEMYIQGLSTSMPKHIQEQM
VRSLPGMENARIVRYAYAIEYDAINPRQLYQSLETKVIHNLFCAGQINGTSGYEEAAGQGLMAGINAGLKVQGKKPLVLK
RDEAYIGVLIDDLITKGTSEPYRLLTSRAEHRLLLRNDNADIRLRDYGYQIGLVDDATYERFEHKKIALKEMIERAKSTK
INPTEENLNYLASVNSSPIYEGVTVFKLLERPELKIETLKHFLPSDYNHEIYEQLEIYIKYDGYIEKARREADKLLRYES
RFIPNDINYHSIHNISAEAKEKLSKIKPETLGQATRILGVGPTDVSMLLVYLEAKNA

Sequences:

>Translated_617_residues
MIYDGIIVGAGHAGVEAALAMAKMKLNTLLITGDLTKVATLPCNPSIGGPAKGIVVREIDALGGQMAKSADLSQIQMKML
NPSKGPAVRALRAQIDKLKYPKVMLDVLQNTPNLTLLQGFVDTLIVEDNTVKGVNLEDGSQVFAQTVIITTGTYLGSQIL
IGHEKTSSGPNGERTTRGISNQLKNLGFEMLRLKTGTPPRIARDSIDYSKTVPQPGDGVFQTFSHDSPITDLGHQEFSYL
IHTSPDTKDIIFNNLDASAMYGGVVEGVGPRYCPSIEDKFVRFKDKDRHQIFIEPESMDLNEMYIQGLSTSMPKHIQEQM
VRSLPGMENARIVRYAYAIEYDAINPRQLYQSLETKVIHNLFCAGQINGTSGYEEAAGQGLMAGINAGLKVQGKKPLVLK
RDEAYIGVLIDDLITKGTSEPYRLLTSRAEHRLLLRNDNADIRLRDYGYQIGLVDDATYERFEHKKIALKEMIERAKSTK
INPTEENLNYLASVNSSPIYEGVTVFKLLERPELKIETLKHFLPSDYNHEIYEQLEIYIKYDGYIEKARREADKLLRYES
RFIPNDINYHSIHNISAEAKEKLSKIKPETLGQATRILGVGPTDVSMLLVYLEAKNA
>Mature_617_residues
MIYDGIIVGAGHAGVEAALAMAKMKLNTLLITGDLTKVATLPCNPSIGGPAKGIVVREIDALGGQMAKSADLSQIQMKML
NPSKGPAVRALRAQIDKLKYPKVMLDVLQNTPNLTLLQGFVDTLIVEDNTVKGVNLEDGSQVFAQTVIITTGTYLGSQIL
IGHEKTSSGPNGERTTRGISNQLKNLGFEMLRLKTGTPPRIARDSIDYSKTVPQPGDGVFQTFSHDSPITDLGHQEFSYL
IHTSPDTKDIIFNNLDASAMYGGVVEGVGPRYCPSIEDKFVRFKDKDRHQIFIEPESMDLNEMYIQGLSTSMPKHIQEQM
VRSLPGMENARIVRYAYAIEYDAINPRQLYQSLETKVIHNLFCAGQINGTSGYEEAAGQGLMAGINAGLKVQGKKPLVLK
RDEAYIGVLIDDLITKGTSEPYRLLTSRAEHRLLLRNDNADIRLRDYGYQIGLVDDATYERFEHKKIALKEMIERAKSTK
INPTEENLNYLASVNSSPIYEGVTVFKLLERPELKIETLKHFLPSDYNHEIYEQLEIYIKYDGYIEKARREADKLLRYES
RFIPNDINYHSIHNISAEAKEKLSKIKPETLGQATRILGVGPTDVSMLLVYLEAKNA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=632, Percent_Identity=41.2974683544304, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI19882217, Length=657, Percent_Identity=39.7260273972603, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI183227703, Length=671, Percent_Identity=38.8971684053651, Blast_Score=414, Evalue=1e-115,
Organism=Escherichia coli, GI2367273, Length=616, Percent_Identity=46.9155844155844, Blast_Score=554, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI17534255, Length=615, Percent_Identity=37.2357723577236, Blast_Score=405, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6321202, Length=629, Percent_Identity=43.0842607313196, Blast_Score=476, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24658174, Length=636, Percent_Identity=40.4088050314465, Blast_Score=425, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_ACHLI (A9NEC4)

Other databases:

- EMBL:   CP000896
- RefSeq:   YP_001620080.1
- ProteinModelPortal:   A9NEC4
- SMR:   A9NEC4
- GeneID:   5803628
- GenomeReviews:   CP000896_GR
- KEGG:   acl:ACL_0063
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   ALAI441768:ACL_0063-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 68729; Mature: 68729

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: BINDING 121-121 BINDING 176-176 BINDING 366-366

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYDGIIVGAGHAGVEAALAMAKMKLNTLLITGDLTKVATLPCNPSIGGPAKGIVVREID
CCCCCEEEECCCHHHHHHHHHHHHEEEEEEEECCCCEEEEECCCCCCCCCCCCEEEEEHH
ALGGQMAKSADLSQIQMKMLNPSKGPAVRALRAQIDKLKYPKVMLDVLQNTPNLTLLQGF
HHCCHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHH
VDTLIVEDNTVKGVNLEDGSQVFAQTVIITTGTYLGSQILIGHEKTSSGPNGERTTRGIS
HHHEEECCCEEECCCCCCCHHHHHEEEEEEECCCCCCEEEEECCCCCCCCCCCHHHHHHH
NQLKNLGFEMLRLKTGTPPRIARDSIDYSKTVPQPGDGVFQTFSHDSPITDLGHQEFSYL
HHHHHCCCEEEEEECCCCCCHHHCCCCHHHCCCCCCCCHHHHCCCCCCCCCCCCCCEEEE
IHTSPDTKDIIFNNLDASAMYGGVVEGVGPRYCPSIEDKFVRFKDKDRHQIFIEPESMDL
EECCCCCHHHEECCCCCHHHHCCHHHCCCCCCCCCHHHHHEECCCCCCCEEEECCCCCCH
NEMYIQGLSTSMPKHIQEQMVRSLPGMENARIVRYAYAIEYDAINPRQLYQSLETKVIHN
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHH
LFCAGQINGTSGYEEAAGQGLMAGINAGLKVQGKKPLVLKRDEAYIGVLIDDLITKGTSE
HHEECCCCCCCCHHHHHCCCEEEECCCCEEECCCCCEEEECCCCEEEHHHHHHHHCCCCC
PYRLLTSRAEHRLLLRNDNADIRLRDYGYQIGLVDDATYERFEHKKIALKEMIERAKSTK
HHHHHHHHCCCEEEEECCCCCEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCC
INPTEENLNYLASVNSSPIYEGVTVFKLLERPELKIETLKHFLPSDYNHEIYEQLEIYIK
CCCCHHHHHHHEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHEEEEEEEE
YDGYIEKARREADKLLRYESRFIPNDINYHSIHNISAEAKEKLSKIKPETLGQATRILGV
ECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHCCHHHEEEC
GPTDVSMLLVYLEAKNA
CCCCCEEEEEEEECCCC
>Mature Secondary Structure
MIYDGIIVGAGHAGVEAALAMAKMKLNTLLITGDLTKVATLPCNPSIGGPAKGIVVREID
CCCCCEEEECCCHHHHHHHHHHHHEEEEEEEECCCCEEEEECCCCCCCCCCCCEEEEEHH
ALGGQMAKSADLSQIQMKMLNPSKGPAVRALRAQIDKLKYPKVMLDVLQNTPNLTLLQGF
HHCCHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHH
VDTLIVEDNTVKGVNLEDGSQVFAQTVIITTGTYLGSQILIGHEKTSSGPNGERTTRGIS
HHHEEECCCEEECCCCCCCHHHHHEEEEEEECCCCCCEEEEECCCCCCCCCCCHHHHHHH
NQLKNLGFEMLRLKTGTPPRIARDSIDYSKTVPQPGDGVFQTFSHDSPITDLGHQEFSYL
HHHHHCCCEEEEEECCCCCCHHHCCCCHHHCCCCCCCCHHHHCCCCCCCCCCCCCCEEEE
IHTSPDTKDIIFNNLDASAMYGGVVEGVGPRYCPSIEDKFVRFKDKDRHQIFIEPESMDL
EECCCCCHHHEECCCCCHHHHCCHHHCCCCCCCCCHHHHHEECCCCCCCEEEECCCCCCH
NEMYIQGLSTSMPKHIQEQMVRSLPGMENARIVRYAYAIEYDAINPRQLYQSLETKVIHN
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHH
LFCAGQINGTSGYEEAAGQGLMAGINAGLKVQGKKPLVLKRDEAYIGVLIDDLITKGTSE
HHEECCCCCCCCHHHHHCCCEEEECCCCEEECCCCCEEEECCCCEEEHHHHHHHHCCCCC
PYRLLTSRAEHRLLLRNDNADIRLRDYGYQIGLVDDATYERFEHKKIALKEMIERAKSTK
HHHHHHHHCCCEEEEECCCCCEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCC
INPTEENLNYLASVNSSPIYEGVTVFKLLERPELKIETLKHFLPSDYNHEIYEQLEIYIK
CCCCHHHHHHHEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHEEEEEEEE
YDGYIEKARREADKLLRYESRFIPNDINYHSIHNISAEAKEKLSKIKPETLGQATRILGV
ECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHCCHHHEEEC
GPTDVSMLLVYLEAKNA
CCCCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA