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The map label for this gene is rnfD [H]

Identifier: 162446935

GI number: 162446935

Start: 59717

End: 60787

Strand: Direct

Name: rnfD [H]

Synonym: ACL_0049

Alternate gene names: 162446935

Gene position: 59717-60787 (Clockwise)

Preceding gene: 162446934

Following gene: 162446936

Centisome position: 3.99

GC content: 34.08

Gene sequence:

>1071_bases
ATGCAAAATATATCTATTTTAAGCCAAACATCACCGTATATACGTAAAGAAACTTCTACAAAACGTATGATGTTTGATGT
GTTAATTGCACTAACGCCTGTAGTATTCTTTTCAATCTACAGATTTGGATTAGATGCATTAGCAAGAATATTAGTGAGTT
TAATTATCTTTGTTGGTGTGGAAGCTCTATATTTTTTAAGTGTTACGCGTGTAGATGGTTATAACTTCAAAGATAAAATT
AAAAATAAGTTTAAAAAATACAGTATAAATAACTTTAGTGCTCCTGCAGTATCTGCAGTCATCTATGCGATGTTATTACC
AAACCAACTACATCTTTATGTGGTTGTTATGGGGGCTTTATTTGGTGCTGCAATCGGCAAAATGATTTTTGGTGGTTTAG
GGATGAATATCTTTAACCCTGCAGCAGCTGGCCGTGTATTTATTGCACTAGCATTTACAACATTCTTCACAGGTTCTTAT
GGGTTTATTGATGCTGCAGCAGGTGCTACAGCATTATCTACAAGTTTTCCTGAAGTATTAAATAGTTATTCACTAATCGA
TTTACTCGTTGGTAATATTCCGGGTTCTATGGGTGAAATCAATTCCCTTGCTATTTTATTTGGTCTTGCTTATCTATTAA
TTAGAAAAAGTGCAGATTATAGACCTGTTTTATCAGGACTATTAATCTTTACAGCACTTGCTACAGTTGCAGGATTCATA
CTACACCCAACATTAGTATTTGAATATGTGATATTCCAATTATTATCTGGTGGTTTATTATTTGGTCTAACCTTTATGAT
TACAGACCCTGCGACATCACCAATCACAAGACCAGGTCGTTGGTGGTTTGGTTTAATTGTTGGTTCATTAGTATTCTTTA
TTAGAAGTTTTGGTAGTCTTCCAGAAGGTGTGGCGTTTGCTTTATTATTTGGTAACTTAATGGTACCTTTACTAGATTAC
CCAATGTGGGCAAACAACAGATTTACAAAGAAATTCTATATTAGCTTTAGTTTAGTATTTGTGGCTTTAGTAGCACTTAC
CTTCGTGATTTTAGGAGGTTACGCGATATGA

Upstream 100 bases:

>100_bases
ATAAATGTATTGAATGTGGATTATGTTCATTTGTATGTCCATCTAAGATTCATTTAACAGATTACATGCGTGAAGGTAAA
CGCATCTTAAGAGGTTAATT

Downstream 100 bases:

>100_bases
CAAAAGCAGTTAAAAGCATCATTACATTAGCGGTTGTAGCTATTTTAGGTATTGGACTATCAATTGGTACGCACTTCATC
TTTAATCCAATTAAAGAAGA

Product: NADH oxidoreductase complex RnfABCDGE type, D subunit

Products: NA

Alternate protein names: Na(+)-NQR subunit B; Na(+)-translocating NQR subunit B; NQR complex subunit B; NQR-1 subunit B [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MQNISILSQTSPYIRKETSTKRMMFDVLIALTPVVFFSIYRFGLDALARILVSLIIFVGVEALYFLSVTRVDGYNFKDKI
KNKFKKYSINNFSAPAVSAVIYAMLLPNQLHLYVVVMGALFGAAIGKMIFGGLGMNIFNPAAAGRVFIALAFTTFFTGSY
GFIDAAAGATALSTSFPEVLNSYSLIDLLVGNIPGSMGEINSLAILFGLAYLLIRKSADYRPVLSGLLIFTALATVAGFI
LHPTLVFEYVIFQLLSGGLLFGLTFMITDPATSPITRPGRWWFGLIVGSLVFFIRSFGSLPEGVAFALLFGNLMVPLLDY
PMWANNRFTKKFYISFSLVFVALVALTFVILGGYAI

Sequences:

>Translated_356_residues
MQNISILSQTSPYIRKETSTKRMMFDVLIALTPVVFFSIYRFGLDALARILVSLIIFVGVEALYFLSVTRVDGYNFKDKI
KNKFKKYSINNFSAPAVSAVIYAMLLPNQLHLYVVVMGALFGAAIGKMIFGGLGMNIFNPAAAGRVFIALAFTTFFTGSY
GFIDAAAGATALSTSFPEVLNSYSLIDLLVGNIPGSMGEINSLAILFGLAYLLIRKSADYRPVLSGLLIFTALATVAGFI
LHPTLVFEYVIFQLLSGGLLFGLTFMITDPATSPITRPGRWWFGLIVGSLVFFIRSFGSLPEGVAFALLFGNLMVPLLDY
PMWANNRFTKKFYISFSLVFVALVALTFVILGGYAI
>Mature_356_residues
MQNISILSQTSPYIRKETSTKRMMFDVLIALTPVVFFSIYRFGLDALARILVSLIIFVGVEALYFLSVTRVDGYNFKDKI
KNKFKKYSINNFSAPAVSAVIYAMLLPNQLHLYVVVMGALFGAAIGKMIFGGLGMNIFNPAAAGRVFIALAFTTFFTGSY
GFIDAAAGATALSTSFPEVLNSYSLIDLLVGNIPGSMGEINSLAILFGLAYLLIRKSADYRPVLSGLLIFTALATVAGFI
LHPTLVFEYVIFQLLSGGLLFGLTFMITDPATSPITRPGRWWFGLIVGSLVFFIRSFGSLPEGVAFALLFGNLMVPLLDY
PMWANNRFTKKFYISFSLVFVALVALTFVILGGYAI

Specific function: NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm. NqrA to nqrE are probably involved in the second step, the conversion of ubisemiqui

COG id: COG4658

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfD

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrB/rnfD family [H]

Homologues:

Organism=Escherichia coli, GI1787917, Length=350, Percent_Identity=30.8571428571429, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010966
- InterPro:   IPR004338 [H]

Pfam domain/function: PF03116 NQR2_RnfD_RnfE [H]

EC number: NA

Molecular weight: Translated: 38972; Mature: 38972

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNISILSQTSPYIRKETSTKRMMFDVLIALTPVVFFSIYRFGLDALARILVSLIIFVGV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EALYFLSVTRVDGYNFKDKIKNKFKKYSINNFSAPAVSAVIYAMLLPNQLHLYVVVMGAL
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
FGAAIGKMIFGGLGMNIFNPAAAGRVFIALAFTTFFTGSYGFIDAAAGATALSTSFPEVL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHH
NSYSLIDLLVGNIPGSMGEINSLAILFGLAYLLIRKSADYRPVLSGLLIFTALATVAGFI
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LHPTLVFEYVIFQLLSGGLLFGLTFMITDPATSPITRPGRWWFGLIVGSLVFFIRSFGSL
HHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PEGVAFALLFGNLMVPLLDYPMWANNRFTKKFYISFSLVFVALVALTFVILGGYAI
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQNISILSQTSPYIRKETSTKRMMFDVLIALTPVVFFSIYRFGLDALARILVSLIIFVGV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EALYFLSVTRVDGYNFKDKIKNKFKKYSINNFSAPAVSAVIYAMLLPNQLHLYVVVMGAL
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
FGAAIGKMIFGGLGMNIFNPAAAGRVFIALAFTTFFTGSYGFIDAAAGATALSTSFPEVL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHH
NSYSLIDLLVGNIPGSMGEINSLAILFGLAYLLIRKSADYRPVLSGLLIFTALATVAGFI
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LHPTLVFEYVIFQLLSGGLLFGLTFMITDPATSPITRPGRWWFGLIVGSLVFFIRSFGSL
HHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PEGVAFALLFGNLMVPLLDYPMWANNRFTKKFYISFSLVFVALVALTFVILGGYAI
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA