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The map label for this gene is glyA

Identifier: 162446933

GI number: 162446933

Start: 57170

End: 58399

Strand: Direct

Name: glyA

Synonym: ACL_0047

Alternate gene names: 162446933

Gene position: 57170-58399 (Clockwise)

Preceding gene: 162446932

Following gene: 162446934

Centisome position: 3.82

GC content: 33.9

Gene sequence:

>1230_bases
ATGACATTAAAAGACTATGATCTTGAATTATTTAACGCGATTCAAAGAGAAGATAACAGACAAAAAGAACACATCGAATT
AATTGCGTCCGAAAACTTTGTTTCTGATGCAGTGTTAGAGGCACAAGGTTCAATTTTAACCAACAAATATGCAGAAGGCT
ATCCAAATAAAAGATATTATGGAGGTTGCGAATTTGTTGATCAAGTAGAGATACTAGCTCAAGATAGGTTAAAACAAATC
TTTAATGCGAAGTTTGTAAATGTACAGCCTCATTCGGGTTCACAGGCTAATGCTGCTGTTTATCAGGCACTACTTAGTCC
CGGAGACAGGGTTTTAGGAATGGATTTAAATGCGGGTGGTCACTTAACTCATGGTTATAAATTATCATTTTCTGGACATT
ATTATGAAGCACATGCCTATGGTGTAAGTAGGTTTGATGAAAGAATTGATTATGAAGAAGTACTGAAAATTGCAATTGAA
GTTAAACCTAAAATGATTATTGCAGGTGCGAGTGCATATCCAAGAGTCATTGATTTTAAAAAGTTTAGAGAAATTGCAGA
TACAGTTGGTGCATACTTGTTTGTAGATATGGCACATATTGCAGGACTAGTTGCTTGTGGATTACATCCATCACCACTAC
CTTATGCAGATGTTGTCACGTCTACTACACATAAAACCTTAAGAGGTCCACGTGGTGGTATTATACTTACAAATGATGCA
AGTATTGCTAAAAAAATAGATCGTGCAGTATTTCCTGGCCAACAAGGTGGTCCATTAATGCACATTATTGCTGCAAAGGC
GGTTGCATTTAAAGAAGCACTAGACCCTAACTTTAAGGTATATCAAACACAAGTGATTAAAAATGCTAAAGCCTTAAGTG
ATACATTCAAATCACTTGGATATAAATTAATTTCAGATGGTACAGATAATCATTTAATCTTAGTAGATGTTAAATCAAAA
TTAGGTATTACAGGAAGAGATGCTGAGGATGCGCTATATAAAGCAAATATCACAATCAATAAAAACCAGTTACCGTTTGA
TCAAGAAAAACCGATGTTAACCTCAGGTATAAGATTAGGAACACCTGCGATGACAACTAAGGGTTTTAAAGAAAATGAGT
TTATTAAAGTAGCACAACTTATTGATGAAGTGTTATCGAATATAAACAATGAAGAAGTTATCAATAAAGTTAAAAAAGAA
GTACTAAAACTAATGAAAGATGTTAAATGA

Upstream 100 bases:

>100_bases
TTACGCATTTTTTGTTATGTCCAAATCACCATTGCAATGTATAAATATATTTATACATGGTAAAATATAATGAAAAGATA
TTTTGCGGTGAGGTATAACT

Downstream 100 bases:

>100_bases
AAAGGAGTGATTTTCTTTGATCACAAGAACAAGATATATACCTGATGGTAAAGATGTTTTAAAGAAACTACCATTAATCC
ATCACTTAGAAGCACCATAT

Product: glycine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MTLKDYDLELFNAIQREDNRQKEHIELIASENFVSDAVLEAQGSILTNKYAEGYPNKRYYGGCEFVDQVEILAQDRLKQI
FNAKFVNVQPHSGSQANAAVYQALLSPGDRVLGMDLNAGGHLTHGYKLSFSGHYYEAHAYGVSRFDERIDYEEVLKIAIE
VKPKMIIAGASAYPRVIDFKKFREIADTVGAYLFVDMAHIAGLVACGLHPSPLPYADVVTSTTHKTLRGPRGGIILTNDA
SIAKKIDRAVFPGQQGGPLMHIIAAKAVAFKEALDPNFKVYQTQVIKNAKALSDTFKSLGYKLISDGTDNHLILVDVKSK
LGITGRDAEDALYKANITINKNQLPFDQEKPMLTSGIRLGTPAMTTKGFKENEFIKVAQLIDEVLSNINNEEVINKVKKE
VLKLMKDVK

Sequences:

>Translated_409_residues
MTLKDYDLELFNAIQREDNRQKEHIELIASENFVSDAVLEAQGSILTNKYAEGYPNKRYYGGCEFVDQVEILAQDRLKQI
FNAKFVNVQPHSGSQANAAVYQALLSPGDRVLGMDLNAGGHLTHGYKLSFSGHYYEAHAYGVSRFDERIDYEEVLKIAIE
VKPKMIIAGASAYPRVIDFKKFREIADTVGAYLFVDMAHIAGLVACGLHPSPLPYADVVTSTTHKTLRGPRGGIILTNDA
SIAKKIDRAVFPGQQGGPLMHIIAAKAVAFKEALDPNFKVYQTQVIKNAKALSDTFKSLGYKLISDGTDNHLILVDVKSK
LGITGRDAEDALYKANITINKNQLPFDQEKPMLTSGIRLGTPAMTTKGFKENEFIKVAQLIDEVLSNINNEEVINKVKKE
VLKLMKDVK
>Mature_408_residues
TLKDYDLELFNAIQREDNRQKEHIELIASENFVSDAVLEAQGSILTNKYAEGYPNKRYYGGCEFVDQVEILAQDRLKQIF
NAKFVNVQPHSGSQANAAVYQALLSPGDRVLGMDLNAGGHLTHGYKLSFSGHYYEAHAYGVSRFDERIDYEEVLKIAIEV
KPKMIIAGASAYPRVIDFKKFREIADTVGAYLFVDMAHIAGLVACGLHPSPLPYADVVTSTTHKTLRGPRGGIILTNDAS
IAKKIDRAVFPGQQGGPLMHIIAAKAVAFKEALDPNFKVYQTQVIKNAKALSDTFKSLGYKLISDGTDNHLILVDVKSKL
GITGRDAEDALYKANITINKNQLPFDQEKPMLTSGIRLGTPAMTTKGFKENEFIKVAQLIDEVLSNINNEEVINKVKKEV
LKLMKDVK

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:
GO:0003824: Serine hydroxymethyltransferase
GO:0004372: Serine hydroxymethyltransferase
GO:0005737: Serine hydroxymethyltransferase
GO:0006544: Serine hydroxymethyltransferase
GO:0006563: Serine hydroxymethyltransferase
GO:0006730: Serine hydroxymethyltransferase
GO:0016740: Serine hydroxymethyltransferase
GO:0030170: Serine hydroxymethyltransferase

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=405, Percent_Identity=48.3950617283951, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI261862352, Length=405, Percent_Identity=48.3950617283951, Blast_Score=374, Evalue=1e-104,
Organism=Homo sapiens, GI261862350, Length=405, Percent_Identity=48.3950617283951, Blast_Score=374, Evalue=1e-104,
Organism=Homo sapiens, GI261862348, Length=405, Percent_Identity=48.3950617283951, Blast_Score=374, Evalue=1e-104,
Organism=Homo sapiens, GI261862346, Length=405, Percent_Identity=47.6543209876543, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI22547186, Length=402, Percent_Identity=49.7512437810945, Blast_Score=355, Evalue=5e-98,
Organism=Homo sapiens, GI22547189, Length=388, Percent_Identity=47.680412371134, Blast_Score=325, Evalue=5e-89,
Organism=Escherichia coli, GI1788902, Length=406, Percent_Identity=60.3448275862069, Blast_Score=476, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI25144732, Length=425, Percent_Identity=47.2941176470588, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI25144729, Length=425, Percent_Identity=47.2941176470588, Blast_Score=366, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6323087, Length=430, Percent_Identity=47.906976744186, Blast_Score=373, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6319739, Length=416, Percent_Identity=45.4326923076923, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24640005, Length=405, Percent_Identity=49.8765432098765, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI221329721, Length=405, Percent_Identity=49.8765432098765, Blast_Score=386, Evalue=1e-107,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_ACHLI (A9NEA9)

Other databases:

- EMBL:   CP000896
- RefSeq:   YP_001620065.1
- ProteinModelPortal:   A9NEA9
- SMR:   A9NEA9
- GeneID:   5803954
- GenomeReviews:   CP000896_GR
- KEGG:   acl:ACL_0047
- HOGENOM:   HBG301263
- OMA:   RAVFPGY
- BioCyc:   ALAI441768:ACL_0047-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45325; Mature: 45193

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKDYDLELFNAIQREDNRQKEHIELIASENFVSDAVLEAQGSILTNKYAEGYPNKRYY
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCC
GGCEFVDQVEILAQDRLKQIFNAKFVNVQPHSGSQANAAVYQALLSPGDRVLGMDLNAGG
CCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEECCCC
HLTHGYKLSFSGHYYEAHAYGVSRFDERIDYEEVLKIAIEVKPKMIIAGASAYPRVIDFK
CCCCCEEEEECCCEEEEHHHHHHHHHHHCCHHHHHHHHHEECCEEEEECCCCCCHHHHHH
KFREIADTVGAYLFVDMAHIAGLVACGLHPSPLPYADVVTSTTHKTLRGPRGGIILTNDA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCH
SIAKKIDRAVFPGQQGGPLMHIIAAKAVAFKEALDPNFKVYQTQVIKNAKALSDTFKSLG
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCC
YKLISDGTDNHLILVDVKSKLGITGRDAEDALYKANITINKNQLPFDQEKPMLTSGIRLG
EEEECCCCCCEEEEEEEHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHCCCCCC
TPAMTTKGFKENEFIKVAQLIDEVLSNINNEEVINKVKKEVLKLMKDVK
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLKDYDLELFNAIQREDNRQKEHIELIASENFVSDAVLEAQGSILTNKYAEGYPNKRYY
CCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCC
GGCEFVDQVEILAQDRLKQIFNAKFVNVQPHSGSQANAAVYQALLSPGDRVLGMDLNAGG
CCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEECCCC
HLTHGYKLSFSGHYYEAHAYGVSRFDERIDYEEVLKIAIEVKPKMIIAGASAYPRVIDFK
CCCCCEEEEECCCEEEEHHHHHHHHHHHCCHHHHHHHHHEECCEEEEECCCCCCHHHHHH
KFREIADTVGAYLFVDMAHIAGLVACGLHPSPLPYADVVTSTTHKTLRGPRGGIILTNDA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCH
SIAKKIDRAVFPGQQGGPLMHIIAAKAVAFKEALDPNFKVYQTQVIKNAKALSDTFKSLG
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCC
YKLISDGTDNHLILVDVKSKLGITGRDAEDALYKANITINKNQLPFDQEKPMLTSGIRLG
EEEECCCCCCEEEEEEEHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHCCCCCC
TPAMTTKGFKENEFIKVAQLIDEVLSNINNEEVINKVKKEVLKLMKDVK
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA